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Entry version 29 (16 Oct 2019)
Sequence version 1 (16 Apr 2014)
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Protein

Arylamine N-acetyltransferase

Gene

nat

Organism
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the ‘protein existence’ evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Catalyzes the transfer of the acetyl group from acetyl coenzyme A to the free amino group of arylamines and hydrazines (PubMed:18795795). Is able to utilize not only acetyl-CoA, but also n-propionyl-CoA and acetoacetyl-CoA as acyl donors, although at a lower rate (PubMed:19014350). As acetyl-CoA and propionyl-CoA are products of cholesterol catabolism and the nat gene is likely present in the same operon than genes involved in cholesterol degradation, this enzyme could have a role in the utilization and regulation of these CoA species (PubMed:19014350).2 Publications
It has been reported that overexpression of this enzyme may be responsible for increased resistance to the front-line antitubercular drug isoniazid (INH), by acetylating and hence inactivating the prodrug (PubMed:9973365). However, isoniazid is an extremely poor substrate for the enzyme; therefore, the expression of TBNAT is unlikely to be a significant cause of isoniazid resistance in M.tuberculosis (PubMed:18795795).2 Publications

Miscellaneous

Was identified as a high-confidence drug target.1 Publication
Reactions proceed via a bi-bi ping-pong kinetic mechanism.1 Publication
Resistance to the antitubercular drug isoniazid (INH) has been associated to mutations in this gene in some INH-resistant clinical isolates of M.tuberculosis.1 Publication

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the ‘Function’ section describes biophysical and chemical properties, such as maximal absorption, kinetic parameters, pH dependence, redox potentials and temperature dependence.<p><a href='/help/biophysicochemical_properties' target='_top'>More...</a></p>Kineticsi

kcat is 94 sec(-1) with 3-amino-4-hydroxybenzoic acid as substrate. kcat is 66 sec(-1) with 4-amino-3-hydroxybenzoic acid as substrate. kcat is 44 sec(-1) with hydralazine as substrate. kcat is 70 sec(-1) with 2-amino-4-methylphenol as substrate. kcat is 21 sec(-1) with 2-amino-4-chlorophenol as substrate. kcat is 51 sec(-1) with 2-aminophenol as substrate. kcat is 10.9 sec(-1) with 4-hydroxybenzhydrazide as substrate. kcat is 2.9 sec(-1) with anisidine as substrate. kcat is 0.7 sec(-1) with p-aminobenzoic acid as substrate. kcat is 1.9 sec(-1) with benzoic acid hydrazide as substrate. kcat is 0.8 sec(-1) with 4-methylaniline as substrate. kcat is 4.9 sec(-1) with isoniazid as substrate. kcat is 0.6 sec(-1) with nicotinic acid hydrazide as substrate. kcat is 0.2 sec(-1) with 4-chloroaniline as substrate. kcat is 0.28 sec(-1) with aniline as substrate. kcat is 0.9 sec(-1) with 4-fluoroaniline as substrate.1 Publication
  1. KM=0.14 mM for acetyl-CoA1 Publication
  2. KM=0.56 mM for acetyl-CoA1 Publication
  3. KM=0.29 mM for n-propionyl-CoA1 Publication
  4. KM=0.32 mM for 3-amino-4-hydroxybenzoic acid1 Publication
  5. KM=0.90 mM for 4-amino-3-hydroxybenzoic acid1 Publication
  6. KM=0.61 mM for hydralazine1 Publication
  7. KM=3.06 mM for 2-amino-4-methylphenol1 Publication
  8. KM=1.9 mM for 2-amino-4-chlorophenol1 Publication
  9. KM=5.79 mM for 2-aminophenol1 Publication
  10. KM=20 mM for 4-hydroxybenzhydrazide1 Publication
  11. KM=14 mM for anisidine1 Publication
  12. KM=5.0 mM for p-aminobenzoic acid1 Publication
  13. KM=14 mM for benzoic acid hydrazide1 Publication
  14. KM=11 mM for 4-methylaniline1 Publication
  15. KM=102 mM for isoniazid1 Publication
  16. KM=14 mM for nicotinic acid hydrazide1 Publication
  17. KM=6 mM for 4-chloroaniline1 Publication
  18. KM=23 mM for aniline1 Publication
  19. KM=51 mM for 4-fluoroaniline1 Publication

    Temperature dependencei

    Is thermostable. Retains >95% of its activity after incubation at 60 degrees Celsius for 30 minutes.1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section is used for enzymes and indicates the residues directly involved in catalysis.<p><a href='/help/act_site' target='_top'>More...</a></p>Active sitei70Acyl-thioester intermediate1 Publication1
    Active sitei110Proton acceptor1 Publication1
    Active sitei1271 Publication1

    <p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

    • arylamine N-acetyltransferase activity Source: MTBBASE

    GO - Biological processi

    <p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

    Molecular functionAcyltransferase, Transferase
    Biological processAntibiotic resistance

    Enzyme and pathway databases

    BioCyc Collection of Pathway/Genome Databases

    More...
    BioCyci
    MTBH37RV:G185E-7843-MONOMER

    <p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

    <p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
    Recommended name:
    Arylamine N-acetyltransferase1 Publication (EC:2.3.1.52 Publications)
    Short name:
    NAT1 Publication
    Alternative name(s):
    TBNAT2 Publications
    <p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: ‘Name’, ‘Synonyms’, ‘Ordered locus names’ and ‘ORF names’.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
    Name:nat1 Publication
    Synonyms:nhoA, tbnat
    Ordered Locus Names:Rv3566c
    ORF Names:MTCY06G11.13c
    <p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiMycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
    <p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the ‘taxonomic identifier’ or ‘taxid’.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri83332 [NCBI]
    <p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
    <p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
    • UP000001584 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes_manual">proteome</a> can consist of several components. <br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome

    Organism-specific databases

    Mycobacterium tuberculosis strain H37Rv genome database

    More...
    TubercuListi
    Rv3566c

    <p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

    GO - Cellular componenti

    <p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

    Chemistry databases

    Drug and drug target database

    More...
    DrugBanki
    DB00515 Cisplatin
    DB00951 Isoniazid
    DB00244 Mesalazine
    DB01582 Sulfamethazine

    <p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the ‘PTM / Processing’ section describes the extent of a polypeptide chain in the mature protein following processing.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00001079191 – 283Arylamine N-acetyltransferaseAdd BLAST283

    Proteomic databases

    PaxDb, a database of protein abundance averages across all three domains of life

    More...
    PaxDbi
    P9WJI5

    PRoteomics IDEntifications database

    More...
    PRIDEi
    P9WJI5

    <p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

    <p>This subsection of the <a href="http://www.uniprot.org/help/interaction_section">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

    Homodimer and homotetramer.

    By similarity

    Protein-protein interaction databases

    STRING: functional protein association networks

    More...
    STRINGi
    83332.Rv3566c

    <p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

    3D structure databases

    SWISS-MODEL Repository - a database of annotated 3D protein structure models

    More...
    SMRi
    P9WJI5

    Database of comparative protein structure models

    More...
    ModBasei
    Search...

    <p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

    <p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

    Phylogenomic databases

    evolutionary genealogy of genes: Non-supervised Orthologous Groups

    More...
    eggNOGi
    ENOG4108MUX Bacteria
    COG2162 LUCA

    KEGG Orthology (KO)

    More...
    KOi
    K00622

    Identification of Orthologs from Complete Genome Data

    More...
    OMAi
    HPVPFNG

    Database for complete collections of gene phylogenies

    More...
    PhylomeDBi
    P9WJI5

    Family and domain databases

    Integrated resource of protein families, domains and functional sites

    More...
    InterProi
    View protein in InterPro
    IPR001447 Arylamine_N-AcTrfase
    IPR038765 Papain-like_cys_pep_sf

    The PANTHER Classification System

    More...
    PANTHERi
    PTHR11786 PTHR11786, 1 hit

    Pfam protein domain database

    More...
    Pfami
    View protein in Pfam
    PF00797 Acetyltransf_2, 1 hit

    Superfamily database of structural and functional annotation

    More...
    SUPFAMi
    SSF54001 SSF54001, 1 hit

    <p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

    <p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

    P9WJI5-1 [UniParc]FASTAAdd to basket
    « Hide
            10         20         30         40         50
    MALDLTAYFD RINYRGATDP TLDVLQDLVT VHSRTIPFEN LDPLLGVPVD
    60 70 80 90 100
    DLSPQALADK LVLRRRGGYC FEHNGLMGYV LAELGYRVRR FAARVVWKLA
    110 120 130 140 150
    PDAPLPPQTH TLLGVTFPGS GGCYLVDVGF GGQTPTSPLR LETGAVQPTT
    160 170 180 190 200
    HEPYRLEDRV DGFVLQAMVR DTWQTLYEFT TQTRPQIDLK VASWYASTHP
    210 220 230 240 250
    ASKFVTGLTA AVITDDARWN LSGRDLAVHR AGGTEKIRLA DAAAVVDTLS
    260 270 280
    ERFGINVADI GERGALETRI DELLARQPGA DAP
    Length:283
    Mass (Da):31,029
    Last modified:April 16, 2014 - v1
    <p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i9C8D98E3256D088A
    GO

    Sequence databases

    Select the link destinations:

    EMBL nucleotide sequence database

    More...
    EMBLi

    GenBank nucleotide sequence database

    More...
    GenBanki

    DNA Data Bank of Japan; a nucleotide sequence database

    More...
    DDBJi
    Links Updated
    AL123456 Genomic DNA Translation: CCP46388.1

    NCBI Reference Sequences

    More...
    RefSeqi
    WP_003419364.1, NZ_NVQJ01000014.1
    YP_177989.1, NC_000962.3

    Genome annotation databases

    Ensembl bacterial and archaeal genome annotation project

    More...
    EnsemblBacteriai
    CCP46388; CCP46388; Rv3566c

    Database of genes from NCBI RefSeq genomes

    More...
    GeneIDi
    888005

    KEGG: Kyoto Encyclopedia of Genes and Genomes

    More...
    KEGGi
    mtu:Rv3566c
    mtv:RVBD_3566c

    <p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

    <p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AL123456 Genomic DNA Translation: CCP46388.1
    RefSeqiWP_003419364.1, NZ_NVQJ01000014.1
    YP_177989.1, NC_000962.3

    3D structure databases

    SMRiP9WJI5
    ModBaseiSearch...

    Protein-protein interaction databases

    STRINGi83332.Rv3566c

    Chemistry databases

    DrugBankiDB00515 Cisplatin
    DB00951 Isoniazid
    DB00244 Mesalazine
    DB01582 Sulfamethazine

    Proteomic databases

    PaxDbiP9WJI5
    PRIDEiP9WJI5

    Genome annotation databases

    EnsemblBacteriaiCCP46388; CCP46388; Rv3566c
    GeneIDi888005
    KEGGimtu:Rv3566c
    mtv:RVBD_3566c

    Organism-specific databases

    TubercuListiRv3566c

    Phylogenomic databases

    eggNOGiENOG4108MUX Bacteria
    COG2162 LUCA
    KOiK00622
    OMAiHPVPFNG
    PhylomeDBiP9WJI5

    Enzyme and pathway databases

    BioCyciMTBH37RV:G185E-7843-MONOMER

    Family and domain databases

    InterProiView protein in InterPro
    IPR001447 Arylamine_N-AcTrfase
    IPR038765 Papain-like_cys_pep_sf
    PANTHERiPTHR11786 PTHR11786, 1 hit
    PfamiView protein in Pfam
    PF00797 Acetyltransf_2, 1 hit
    SUPFAMiSSF54001 SSF54001, 1 hit

    ProtoNet; Automatic hierarchical classification of proteins

    More...
    ProtoNeti
    Search...

    MobiDB: a database of protein disorder and mobility annotations

    More...
    MobiDBi
    Search...

    <p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

    <p>This subsection of the ‘Entry information’ section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiNAT_MYCTU
    <p>This subsection of the ‘Entry information’ section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called ‘Primary (citable) accession number’.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P9WJI5
    Secondary accession number(s): L0TCY1, P0A5L8, P96848
    <p>This subsection of the ‘Entry information’ section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification (‘Last modified’). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 16, 2014
    Last sequence update: April 16, 2014
    Last modified: October 16, 2019
    This is version 29 of the entry and version 1 of the sequence. See complete history.
    <p>This subsection of the ‘Entry information’ section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    <p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh
      Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh: entries and gene names
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