UniProtKB - P9WJG3 (CARD_MYCTU)
Protein
RNA polymerase-binding transcription factor CarD
Gene
carD
Organism
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Status
Functioni
Controls rRNA transcription by binding to the RNA polymerase (RNAP). Required for replication and persistence during infection of mice.2 Publications
GO - Biological processi
- pathogenesis Source: MTBBASE
- regulation of growth rate Source: UniProtKB
- stringent response Source: MTBBASE
Keywordsi
Biological process | Virulence |
Enzyme and pathway databases
BioCyci | MTBH37RV:G185E-7861-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: RNA polymerase-binding transcription factor CarD |
Gene namesi | Name:carD Ordered Locus Names:Rv3583c |
Organismi | Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) |
Taxonomic identifieri | 83332 [NCBI] |
Taxonomic lineagei | Bacteria › Actinobacteria › Corynebacteriales › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex › |
Proteomesi |
|
Organism-specific databases
TubercuListi | Rv3583c |
Pathology & Biotechi
Disruption phenotypei
Essential for growth, it cannot be disrupted.1 Publication
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 25 | R → E: Weakens interaction with RpoB. Abolishes interaction with RpoB; when associated with E-47. 1 Publication | 1 | |
Mutagenesisi | 47 | R → E: Weakens interaction with RpoB, decreases growth rate and virulence, and increases rifampicin sensitivity. Abolishes interaction with RpoB; when associated with E-25. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000420262 | 1 – 162 | RNA polymerase-binding transcription factor CarDAdd BLAST | 162 |
Proteomic databases
PaxDbi | P9WJG3 |
PRIDEi | P9WJG3 |
Interactioni
Subunit structurei
Interacts directly with the N-terminus of the RNA polymerase subunit beta (RpoB).
1 PublicationBinary interactionsi
With | Entry | #Exp. | IntAct | Notes |
---|---|---|---|---|
P9WGY9 | 5 | EBI-6421028,EBI-6419676 |
Protein-protein interaction databases
IntActi | P9WJG3, 1 interactor |
STRINGi | 83332.Rv3583c |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P9WJG3 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Sequence similaritiesi
Belongs to the CarD family.Curated
Phylogenomic databases
eggNOGi | ENOG4108VJ1 Bacteria COG1329 LUCA |
KOi | K07736 |
OMAi | DMKVMIP |
PhylomeDBi | P9WJG3 |
Family and domain databases
Gene3Di | 1.20.58.1290, 1 hit 2.30.30.840, 1 hit |
InterProi | View protein in InterPro IPR036101 CarD-like/TRCF_dom_sf IPR003711 CarD-like/TRCF_domain IPR042215 CarD-like_C |
Pfami | View protein in Pfam PF02559 CarD_CdnL_TRCF, 1 hit |
SMARTi | View protein in SMART SM01058 CarD_TRCF, 1 hit |
SUPFAMi | SSF141259 SSF141259, 1 hit |
i Sequence
Sequence statusi: Complete.
P9WJG3-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MIFKVGDTVV YPHHGAALVE AIETRTIKGE QKEYLVLKVA QGDLTVRVPA
60 70 80 90 100
ENAEYVGVRD VVGQEGLDKV FQVLRAPHTE EPTNWSRRYK ANLEKLASGD
110 120 130 140 150
VNKVAEVVRD LWRRDQERGL SAGEKRMLAK ARQILVGELA LAESTDDAKA
160
ETILDEVLAA AS
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL123456 Genomic DNA Translation: CCP46406.1 |
PIRi | H70803 |
RefSeqi | NP_218100.1, NC_000962.3 WP_003419482.1, NZ_NVQJ01000014.1 |
Genome annotation databases
EnsemblBacteriai | CCP46406; CCP46406; Rv3583c |
GeneIDi | 41579974 887854 |
KEGGi | mtu:Rv3583c mtv:RVBD_3583c |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL123456 Genomic DNA Translation: CCP46406.1 |
PIRi | H70803 |
RefSeqi | NP_218100.1, NC_000962.3 WP_003419482.1, NZ_NVQJ01000014.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4ILU | X-ray | 2.30 | A | 1-162 | [»] | |
4KBM | X-ray | 2.11 | B | 1-162 | [»] | |
4KMC | X-ray | 2.15 | A | 83-162 | [»] | |
4MFR | X-ray | 2.50 | A | 1-162 | [»] | |
6EDT | electron microscopy | - | M | 1-162 | [»] | |
6EE8 | electron microscopy | 3.92 | M | 1-162 | [»] | |
6EEC | electron microscopy | 3.55 | M | 1-162 | [»] | |
SMRi | P9WJG3 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
IntActi | P9WJG3, 1 interactor |
STRINGi | 83332.Rv3583c |
Proteomic databases
PaxDbi | P9WJG3 |
PRIDEi | P9WJG3 |
Genome annotation databases
EnsemblBacteriai | CCP46406; CCP46406; Rv3583c |
GeneIDi | 41579974 887854 |
KEGGi | mtu:Rv3583c mtv:RVBD_3583c |
Organism-specific databases
TubercuListi | Rv3583c |
Phylogenomic databases
eggNOGi | ENOG4108VJ1 Bacteria COG1329 LUCA |
KOi | K07736 |
OMAi | DMKVMIP |
PhylomeDBi | P9WJG3 |
Enzyme and pathway databases
BioCyci | MTBH37RV:G185E-7861-MONOMER |
Miscellaneous databases
PROi | PR:P9WJG3 |
Family and domain databases
Gene3Di | 1.20.58.1290, 1 hit 2.30.30.840, 1 hit |
InterProi | View protein in InterPro IPR036101 CarD-like/TRCF_dom_sf IPR003711 CarD-like/TRCF_domain IPR042215 CarD-like_C |
Pfami | View protein in Pfam PF02559 CarD_CdnL_TRCF, 1 hit |
SMARTi | View protein in SMART SM01058 CarD_TRCF, 1 hit |
SUPFAMi | SSF141259 SSF141259, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CARD_MYCTU | |
Accessioni | P9WJG3Primary (citable) accession number: P9WJG3 Secondary accession number(s): F2GJS9 Q7D585 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 16, 2014 |
Last sequence update: | April 16, 2014 | |
Last modified: | November 13, 2019 | |
This is version 31 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Complete proteome, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families - Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh
Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references