UniProtKB - P9WJB7 (ESPR_MYCTU)
Protein
Nucleoid-associated protein EspR
Gene
espR
Organism
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Status
Functioni
Virulence regulator that has both architectural and regulatory roles. Impacts cell wall functions and pathogenesis through regulation of multiple genes, including the espACD operon, which is a key ESX-1 component. Influences target gene expression positively or negatively, depending on its binding position relative to the genes it controls. Acts by binding directly to the DNA. May play a central role in regulating virulence gene expression.5 Publications
Caution
Was originally thought to be secreted via the ESX-1 secretion system, but it was probably released in the medium via cell lysis.2 Publications
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
DNA bindingi | 38 – 50 | H-T-H motifSequence analysisAdd BLAST | 13 |
GO - Molecular functioni
- DNA binding Source: MTBBASE
- identical protein binding Source: IntAct
GO - Biological processi
- pathogenesis Source: UniProtKB-KW
- regulation of protein secretion Source: MTBBASE
- regulation of transcription, DNA-templated Source: MTBBASE
- response to host immune response Source: MTBBASE
Keywordsi
Molecular function | Activator, DNA-binding, Repressor |
Biological process | Transcription, Transcription regulation, Virulence |
Names & Taxonomyi
Protein namesi | Recommended name: Nucleoid-associated protein EspRCuratedAlternative name(s): ESX-1 transcriptional regulatory protein EspRCurated |
Gene namesi | Name:espR Ordered Locus Names:Rv3849 |
Organismi | Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) |
Taxonomic identifieri | 83332 [NCBI] |
Taxonomic lineagei | Bacteria › Actinobacteria › Corynebacteriales › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex › |
Proteomesi |
|
Organism-specific databases
TubercuListi | Rv3849 |
Subcellular locationi
- nucleoid 1 Publication
GO - Cellular componenti
- cell wall Source: MTBBASE
- cytosol Source: MTBBASE
- extracellular region Source: MTBBASE
- nucleoid Source: UniProtKB-SubCell
- plasma membrane Source: MTBBASE
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Disruption phenotypei
Mutants exhibit growth defect during infection of mice. They establish a stable infection by three weeks, but the bacterial burden is an order of magnitude less than the wild-type.1 Publication
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 69 | R → A: Affects protein stability. Decreases affinity for DNA and dimerization capacity. 1 Publication | 1 | |
Mutagenesisi | 71 | K → A: Decreases affinity for DNA. Does not affect dimerization. 1 Publication | 1 | |
Mutagenesisi | 100 | R → A: Affects protein stability. Decreases affinity for DNA. Does not affect dimerization. 1 Publication | 1 | |
Mutagenesisi | 105 | R → A: Does not affect DNA binding. 1 Publication | 1 | |
Mutagenesisi | 122 | D → A: Moderate impact on protein stability. Does not affect DNA binding. 1 Publication | 1 | |
Mutagenesisi | 125 | R → A: Moderate impact on protein stability. Does not affect DNA binding. 1 Publication | 1 | |
Mutagenesisi | 131 | D → A: Moderate impact on protein stability. Does not affect DNA binding. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000393905 | 1 – 132 | Nucleoid-associated protein EspRAdd BLAST | 132 |
Proteomic databases
PaxDbi | P9WJB7 |
Expressioni
Inductioni
Negatively autoregulated (PubMed:22479184). Transcriptionally regulated by the MprB/MprA and PhoP/PhoR two-component systems (PubMed:25536998). Expression is growth phase-dependent, peaking in the stationary phase (PubMed:22479184). Probably induced upon phagocytosis (PubMed:18685700).3 Publications
Gene expression databases
CollecTFi | EXPREG_00000c30 |
Interactioni
Subunit structurei
Homodimer. Binds DNA as a dimer of dimers.
2 PublicationsBinary interactionsi
P9WJB7
With | #Exp. | IntAct |
---|---|---|
itself | 3 | EBI-15937970,EBI-15937970 |
GO - Molecular functioni
- identical protein binding Source: IntAct
Protein-protein interaction databases
STRINGi | 83332.Rv3849 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P9WJB7 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domaini
The N-terminal domain binds DNA and the C-terminal domain is involved in dimerization. Both domains are required for transcriptional activity and for ESX-1 function.3 Publications
Phylogenomic databases
eggNOGi | COG1476, Bacteria |
OMAi | AKFFAVP |
Family and domain databases
Gene3Di | 1.10.260.40, 1 hit |
InterProi | View protein in InterPro IPR010982, Lambda_DNA-bd_dom_sf |
i Sequence
Sequence statusi: Complete.
P9WJB7-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSTTFAARLN RLFDTVYPPG RGPHTSAEVI AALKAEGITM SAPYLSQLRS
60 70 80 90 100
GNRTNPSGAT MAALANFFRI KAAYFTDDEY YEKLDKELQW LCTMRDDGVR
110 120 130
RIAQRAHGLP SAAQQKVLDR IDELRRAEGI DA
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL123456 Genomic DNA Translation: CCP46678.1 |
PIRi | F70654 |
RefSeqi | NP_218366.1, NC_000962.3 WP_003399759.1, NZ_NVQJ01000057.1 |
Genome annotation databases
EnsemblBacteriai | CCP46678; CCP46678; Rv3849 |
GeneIDi | 45427853 886184 |
KEGGi | mtu:Rv3849 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL123456 Genomic DNA Translation: CCP46678.1 |
PIRi | F70654 |
RefSeqi | NP_218366.1, NC_000962.3 WP_003399759.1, NZ_NVQJ01000057.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3QF3 | X-ray | 2.41 | A/B/C/D/E/F | 2-132 | [»] | |
3QWG | X-ray | 1.99 | A/B | 2-122 | [»] | |
3QYX | X-ray | 3.75 | A/B/C/D | 2-132 | [»] | |
3R1F | X-ray | 2.50 | A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R | 1-132 | [»] | |
4NDW | X-ray | 3.30 | A/B | 1-132 | [»] | |
SMRi | P9WJB7 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
STRINGi | 83332.Rv3849 |
Proteomic databases
PaxDbi | P9WJB7 |
Genome annotation databases
EnsemblBacteriai | CCP46678; CCP46678; Rv3849 |
GeneIDi | 45427853 886184 |
KEGGi | mtu:Rv3849 |
Organism-specific databases
TubercuListi | Rv3849 |
Phylogenomic databases
eggNOGi | COG1476, Bacteria |
OMAi | AKFFAVP |
Gene expression databases
CollecTFi | EXPREG_00000c30 |
Family and domain databases
Gene3Di | 1.10.260.40, 1 hit |
InterProi | View protein in InterPro IPR010982, Lambda_DNA-bd_dom_sf |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ESPR_MYCTU | |
Accessioni | P9WJB7Primary (citable) accession number: P9WJB7 Secondary accession number(s): L0TDR1, P96228, Q8VIS2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 16, 2014 |
Last sequence update: | April 16, 2014 | |
Last modified: | February 10, 2021 | |
This is version 38 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references