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Protein

NADH-quinone oxidoreductase subunit G

Gene

nuoG

Organism
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be menaquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient (By similarity).By similarity
Plays a critical role in M.tuberculosis ability to inhibit apoptosis of infected macrophages; thus helps the bacterium in its struggle to resist the host immune response (PubMed:17658950). In fact, via a NuoG-dependent mechanism, M.tuberculosis can neutralize NOX2-derived reactive oxygen species (ROS) in order to inhibit TNF-alpha-mediated host cell apoptosis (PubMed:20421951). Also mediates inhibition of neutrophil apoptosis, leading to delayed activation of naive CD4 T cells (PubMed:22264515).3 Publications

Catalytic activityi

NADH + a quinone = NAD+ + a quinol.

Cofactori

Protein has several cofactor binding sites:
  • [2Fe-2S] clusterBy similarityNote: Binds 1 [2Fe-2S] cluster per subunit.By similarity
  • [4Fe-4S] clusterBy similarityNote: Binds 3 [4Fe-4S] clusters per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi49Iron-sulfur 1 (2Fe-2S)By similarity1
Metal bindingi60Iron-sulfur 1 (2Fe-2S)By similarity1
Metal bindingi63Iron-sulfur 1 (2Fe-2S)By similarity1
Metal bindingi77Iron-sulfur 1 (2Fe-2S)By similarity1
Metal bindingi111Iron-sulfur 2 (4Fe-4S); via tele nitrogenPROSITE-ProRule annotation1
Metal bindingi115Iron-sulfur 2 (4Fe-4S)PROSITE-ProRule annotation1
Metal bindingi118Iron-sulfur 2 (4Fe-4S)PROSITE-ProRule annotation1
Metal bindingi124Iron-sulfur 2 (4Fe-4S)PROSITE-ProRule annotation1
Metal bindingi164Iron-sulfur 3 (4Fe-4S)By similarity1
Metal bindingi167Iron-sulfur 3 (4Fe-4S)By similarity1
Metal bindingi170Iron-sulfur 3 (4Fe-4S)By similarity1
Metal bindingi214Iron-sulfur 3 (4Fe-4S)By similarity1
Metal bindingi240Iron-sulfur 4 (4Fe-4S)Sequence analysis1
Metal bindingi243Iron-sulfur 4 (4Fe-4S)Sequence analysis1
Metal bindingi247Iron-sulfur 4 (4Fe-4S)Sequence analysis1
Metal bindingi275Iron-sulfur 4 (4Fe-4S)Sequence analysis1

GO - Molecular functioni

GO - Biological processi

  • ATP synthesis coupled electron transport Source: InterPro
  • negative regulation by symbiont of host apoptotic process Source: MTBBASE
  • pathogenesis Source: MTBBASE

Keywordsi

Molecular functionOxidoreductase
Biological processVirulence
Ligand2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, NAD

Names & Taxonomyi

Protein namesi
Recommended name:
NADH-quinone oxidoreductase subunit G (EC:1.6.5.11)
Alternative name(s):
NADH dehydrogenase I subunit G
NDH-1 subunit G
Gene namesi
Name:nuoG
Ordered Locus Names:Rv3151
ORF Names:MTCY03A2.07c
OrganismiMycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Taxonomic identifieri83332 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
Proteomesi
  • UP000001584 Componenti: Chromosome

Organism-specific databases

TubercuListiRv3151

Subcellular locationi

GO - Cellular componenti

  • cell wall Source: MTBBASE
  • plasma membrane Source: MTBBASE

Pathology & Biotechi

Disruption phenotypei

Deletion of nuoG in M.tuberculosis reduces its ability to inhibit apoptosis of infected human or mouse macrophages and significantly decreases its virulence in mice (PubMed:17658950). The apoptogenic phenotype of the mutant strain is significantly reduced in human macrophages treated with caspase-3 and -8 inhibitors, TNF-alpha-neutralizing antibodies, and also after infection of murine TNF(-/-) macrophages. Moreover, incubation of macrophages with inhibitors of reactive oxygen species (ROS) reduces not only the apoptosis induced by the nuoG deletion mutant, but also its capacity to increase macrophage TNF-alpha secretion. The phagosomes infected with the mutant show increased ROS levels compared to M.tuberculosis phagosomes in primary murine and human alveolar macrophages. The increase in nuoG deletion mutant induced ROS and apoptosis is abolished in NOX-2 deficient (gp91(-/-)) macrophages (PubMed:20421951). Compared to wild-type, the nuoG deletion mutant spreads to a larger number of lung phagocytic cells. Consistent with the shorter lifespan of infected neutrophils, infection with the nuoG mutant results in fewer bacteria per infected neutrophil, accelerated bacterial acquisition by dendritic cells, earlier trafficking of these dendritic cells to lymph nodes, and faster CD4 T cell priming. Neutrophil depletion abrogates accelerated CD4 T cell priming by the nuoG mutant, suggesting that inhibiting neutrophil apoptosis delays adaptive immunity in tuberculosis (PubMed:22264515).3 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00001185592 – 806NADH-quinone oxidoreductase subunit GAdd BLAST805

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylthreonineCombined sources1

Keywords - PTMi

Acetylation, Quinone

Proteomic databases

PaxDbiP9WIV9

PTM databases

iPTMnetiP9WIV9

Interactioni

Subunit structurei

The type I NADH dehydrogenase consists of 14 different subunits.Curated

Protein-protein interaction databases

STRINGi83332.Rv3151

Structurei

3D structure databases

ProteinModelPortaliP9WIV9
SMRiP9WIV9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini15 – 932Fe-2S ferredoxin-typePROSITE-ProRule annotationAdd BLAST79
Domaini95 – 1344Fe-4S His(Cys)3-ligated-typePROSITE-ProRule annotationAdd BLAST40
Domaini233 – 2894Fe-4S Mo/W bis-MGD-typePROSITE-ProRule annotationAdd BLAST57

Sequence similaritiesi

Belongs to the complex I 75 kDa subunit family.Curated

Phylogenomic databases

eggNOGiENOG4108IEH Bacteria
COG1034 LUCA
KOiK00336
OMAiGYGYVNR
PhylomeDBiP9WIV9

Family and domain databases

CDDicd00207 fer2, 1 hit
InterProiView protein in InterPro
IPR036010 2Fe-2S_ferredoxin-like_sf
IPR001041 2Fe-2S_ferredoxin-type
IPR009010 Asp_de-COase-like_dom_sf
IPR006657 MoPterin_dinucl-bd_dom
IPR006656 Mopterin_OxRdtase
IPR006963 Mopterin_OxRdtase_4Fe-4S_dom
IPR000283 NADH_UbQ_OxRdtase_75kDa_su_CS
IPR010228 NADH_UbQ_OxRdtase_Gsu
IPR019574 NADH_UbQ_OxRdtase_Gsu_4Fe4S-bd
PfamiView protein in Pfam
PF00384 Molybdopterin, 1 hit
PF01568 Molydop_binding, 1 hit
PF10588 NADH-G_4Fe-4S_3, 1 hit
SMARTiView protein in SMART
SM00926 Molybdop_Fe4S4, 1 hit
SM00929 NADH-G_4Fe-4S_3, 1 hit
SUPFAMiSSF50692 SSF50692, 1 hit
SSF54292 SSF54292, 1 hit
TIGRFAMsiTIGR01973 NuoG, 1 hit
PROSITEiView protein in PROSITE
PS51085 2FE2S_FER_2, 1 hit
PS51839 4FE4S_HC3, 1 hit
PS51669 4FE4S_MOW_BIS_MGD, 1 hit
PS00641 COMPLEX1_75K_1, 1 hit
PS00642 COMPLEX1_75K_2, 1 hit
PS00643 COMPLEX1_75K_3, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P9WIV9-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MTQAADTDIR VGQPEMVTLT IDGVEISVPK GTLVIRAAEL MGIQIPRFCD
60 70 80 90 100
HPLLEPVGAC RQCLVEVEGQ RKPLASCTTV ATDDMVVRTQ LTSEIADKAQ
110 120 130 140 150
HGVMELLLIN HPLDCPMCDK GGECPLQNQA MSNGRTDSRF TEAKRTFAKP
160 170 180 190 200
INISAQVLLD RERCILCARC TRFSDQIAGD PFIDMQERGA LQQVGIYADE
210 220 230 240 250
PFESYFSGNT VQICPVGALT GTAYRFRARP FDLVSSPSVC EHCASGCAQR
260 270 280 290 300
TDHRRGKVLR RLAGDDPEVN EEWNCDKGRW AFTYATQPDV ITTPLIRDGG
310 320 330 340 350
DPKGALVPTS WSHAMAVAAQ GLAAARGRTG VLVGGRVTWE DAYAYAKFAR
360 370 380 390 400
ITLGTNDIDF RARPHSAEEA DFLAARIAGR HMAVSYADLE SAPVVLLVGF
410 420 430 440 450
EPEDESPIVF LRLRKAARRH RVPVYTIAPF ATGGLHKMSG RLIKTVPGGE
460 470 480 490 500
PAALDDLATG AVGDLLATPG AVIIVGERLA TVPGGLSAAA RLADTTGARL
510 520 530 540 550
AWVPRRAGER GALEAGALPT LLPGGRPLAD EVARAQVCAA WHIAELPAAA
560 570 580 590 600
GRDADGILAA AADETLAALL VGGIEPADFA DPDAVLAALD ATGFVVSLEL
610 620 630 640 650
RHSTVTERAD VVFPVAPTTQ KAGAFVNWEG RYRTFEPALR GSTLQAGQSD
660 670 680 690 700
HRVLDALADD MGVHLGVPTV EAAREELAAL GIWDGKHAAG PHIAATGPTQ
710 720 730 740 750
PEAGEAILTG WRMLLDEGRL QDGEPYLAGT ARTPVVRLSP DTAAEIGAAD
760 770 780 790 800
GEAVTVSTSR GSITLPCSVT DMPDRVVWLP LNSAGSTVHR QLRVTIGSIV

KIGAGS
Length:806
Mass (Da):85,424
Last modified:April 16, 2014 - v1
Checksum:i519A1EA833181064
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL123456 Genomic DNA Translation: CCP45962.1
PIRiH70647
RefSeqiNP_217667.1, NC_000962.3
WP_003916519.1, NZ_NVQJ01000019.1

Genome annotation databases

EnsemblBacteriaiCCP45962; CCP45962; Rv3151
GeneIDi887540
KEGGimtu:Rv3151

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL123456 Genomic DNA Translation: CCP45962.1
PIRiH70647
RefSeqiNP_217667.1, NC_000962.3
WP_003916519.1, NZ_NVQJ01000019.1

3D structure databases

ProteinModelPortaliP9WIV9
SMRiP9WIV9
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi83332.Rv3151

PTM databases

iPTMnetiP9WIV9

Proteomic databases

PaxDbiP9WIV9

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCCP45962; CCP45962; Rv3151
GeneIDi887540
KEGGimtu:Rv3151

Organism-specific databases

TubercuListiRv3151

Phylogenomic databases

eggNOGiENOG4108IEH Bacteria
COG1034 LUCA
KOiK00336
OMAiGYGYVNR
PhylomeDBiP9WIV9

Family and domain databases

CDDicd00207 fer2, 1 hit
InterProiView protein in InterPro
IPR036010 2Fe-2S_ferredoxin-like_sf
IPR001041 2Fe-2S_ferredoxin-type
IPR009010 Asp_de-COase-like_dom_sf
IPR006657 MoPterin_dinucl-bd_dom
IPR006656 Mopterin_OxRdtase
IPR006963 Mopterin_OxRdtase_4Fe-4S_dom
IPR000283 NADH_UbQ_OxRdtase_75kDa_su_CS
IPR010228 NADH_UbQ_OxRdtase_Gsu
IPR019574 NADH_UbQ_OxRdtase_Gsu_4Fe4S-bd
PfamiView protein in Pfam
PF00384 Molybdopterin, 1 hit
PF01568 Molydop_binding, 1 hit
PF10588 NADH-G_4Fe-4S_3, 1 hit
SMARTiView protein in SMART
SM00926 Molybdop_Fe4S4, 1 hit
SM00929 NADH-G_4Fe-4S_3, 1 hit
SUPFAMiSSF50692 SSF50692, 1 hit
SSF54292 SSF54292, 1 hit
TIGRFAMsiTIGR01973 NuoG, 1 hit
PROSITEiView protein in PROSITE
PS51085 2FE2S_FER_2, 1 hit
PS51839 4FE4S_HC3, 1 hit
PS51669 4FE4S_MOW_BIS_MGD, 1 hit
PS00641 COMPLEX1_75K_1, 1 hit
PS00642 COMPLEX1_75K_2, 1 hit
PS00643 COMPLEX1_75K_3, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiNUOG_MYCTU
AccessioniPrimary (citable) accession number: P9WIV9
Secondary accession number(s): L0TBP2, P95175
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 16, 2014
Last sequence update: April 16, 2014
Last modified: November 7, 2018
This is version 31 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh
    Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh: entries and gene names
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Main funding by: National Institutes of Health

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