UniProtKB - P9WIU5 (ARFA_MYCTU)
Protein
Peptidoglycan-binding protein ArfA
Gene
arfA
Organism
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Status
Functioni
Probably plays a role in ammonia secretion that neutralizes the medium at pH 5.5, although it does not play a direct role in ammonia transport. The OmpA-like domain (196-326) binds M.tuberculosis peptidoglycan. Overexpression in M.bovis or M.smegmatis gives channels with average conductance value of 1,600 +/- 100 pS, but this may not be physiologically relevant.3 Publications
Caution
Was originally thought to be a porin.1 Publication
Cofactori
Zn2+Note: May bind Zn2+ via residues in the BON domain.
GO - Molecular functioni
- metal ion binding Source: UniProtKB-KW
- peptidoglycan binding Source: MTBBASE
- porin activity Source: MTBBASE
GO - Biological processi
- ammonium transmembrane transport Source: MTBBASE
- growth of symbiont in host vacuole Source: MTBBASE
- response to acidic pH Source: MTBBASE
Keywordsi
Biological process | Ion transport, Transport |
Ligand | Metal-binding, Zinc |
Names & Taxonomyi
Protein namesi | Recommended name: Peptidoglycan-binding protein ArfAAlternative name(s): Outer membrane porin A Outer membrane protein A Short name: OmpATb Outer membrane protein ArfA |
Gene namesi | Name:arfA Synonyms:ompA Ordered Locus Names:Rv0899 ORF Names:MTCY31.27 |
Organismi | Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) |
Taxonomic identifieri | 83332 [NCBI] |
Taxonomic lineagei | Bacteria › Actinobacteria › Corynebacteriales › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex › |
Proteomesi |
|
Organism-specific databases
TubercuListi | Rv0899 |
Subcellular locationi
- cell wall
- Cell outer membrane Note: Does not have a cleavable signal sequence.
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 30 – 50 | HelicalSequence analysisAdd BLAST | 21 |
GO - Cellular componenti
- cell outer membrane Source: UniProtKB
- cell wall Source: MTBBASE
- cytosol Source: MTBBASE
- extracellular region Source: UniProtKB-KW
- integral component of plasma membrane Source: MTBBASE
Keywords - Cellular componenti
Cell outer membrane, Cell wall, Membrane, SecretedPathology & Biotechi
Disruption phenotypei
Significantly impaired growth at pH 5.5, reduced uptake of serine at both pH 7.2 and 5.5. Reduces growth in macrophages and in intravenously infected mice (PubMed:12366842). But the same mutant has very little effect when studied by another group (PubMed:21410778). Upon operon disruption no reduction of serine uptake at pH 6.9, no visible effect on outer membrane permeability, however severe delays in ammonia secretion, medium pH neutralization and growth also occur at pH 5.5 (PubMed:21410778).2 Publications
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 232 | L → G: Decreases structure stability of OmpA-like domain. 1 Publication | 1 | |
Mutagenesisi | 236 | D → A: Increases conformational stability of OmpA-like domain. Does not alter peptidoglycan-binding. 1 Publication | 1 | |
Mutagenesisi | 277 | R → E: Loss of peptidoglycan-binding; in association with A236. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000196259 | 1 – 326 | Peptidoglycan-binding protein ArfAAdd BLAST | 326 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 208 ↔ 250 | 3 Publications |
Keywords - PTMi
Disulfide bondProteomic databases
PaxDbi | P9WIU5 |
Expressioni
Inductioni
Induced at low pH (at protein level), upon infecting a human monocytic cell line and in murine bone marrow macrophages. Part of the arfA-arfB-arfC operon. Maximal expression of ArfA requires the full operon.2 Publications
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
BMRBi | P9WIU5 |
SMRi | P9WIU5 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 127 – 196 | BONAdd BLAST | 70 | |
Domaini | 212 – 326 | OmpA-likePROSITE-ProRule annotationAdd BLAST | 115 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 73 | Required for protein translocation to the outer membraneAdd BLAST | 73 |
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG2885, Bacteria |
OMAi | MKGEVHN |
Family and domain databases
CDDi | cd07185, OmpA_C-like, 1 hit |
Gene3Di | 3.30.1330.60, 1 hit |
InterProi | View protein in InterPro IPR007055, BON_dom IPR006664, OMP_bac IPR006665, OmpA-like IPR006690, OMPA-like_CS IPR036737, OmpA-like_sf |
Pfami | View protein in Pfam PF04972, BON, 1 hit PF00691, OmpA, 1 hit |
PRINTSi | PR01021, OMPADOMAIN |
SUPFAMi | SSF103088, SSF103088, 1 hit |
PROSITEi | View protein in PROSITE PS01068, OMPA_1, 1 hit PS51123, OMPA_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P9WIU5-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MASKAGLGQT PATTDARRTQ KFYRGSPGRP WLIGAVVIPL LIAAIGYGAF
60 70 80 90 100
ERPQSVTGPT GVLPTLTPTS TRGASALSLS LLSISRSGNT VTLIGDFPDE
110 120 130 140 150
AAKAALMTAL NGLLAPGVNV IDQIHVDPVV RSLDFSSAEP VFTASVPIPD
160 170 180 190 200
FGLKVERDTV TLTGTAPSSE HKDAVKRAAT STWPDMKIVN NIEVTGQAPP
210 220 230 240 250
GPPASGPCAD LQSAINAVTG GPIAFGNDGA SLIPADYEIL NRVADKLKAC
260 270 280 290 300
PDARVTINGY TDNTGSEGIN IPLSAQRAKI VADYLVARGV AGDHIATVGL
310 320
GSVNPIASNA TPEGRAKNRR VEIVVN
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL123456 Genomic DNA Translation: CCP43647.1 |
PIRi | H70782 |
RefSeqi | NP_215414.1, NC_000962.3 WP_003404684.1, NZ_NVQJ01000001.1 |
Genome annotation databases
EnsemblBacteriai | CCP43647; CCP43647; Rv0899 |
GeneIDi | 23492977 885286 |
KEGGi | mtu:Rv0899 mtv:RVBD_0899 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL123456 Genomic DNA Translation: CCP43647.1 |
PIRi | H70782 |
RefSeqi | NP_215414.1, NC_000962.3 WP_003404684.1, NZ_NVQJ01000001.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2KGS | NMR | - | A | 73-204 | [»] | |
2KGW | NMR | - | A | 198-326 | [»] | |
2KSM | NMR | - | A | 73-203 | [»] | |
2L26 | NMR | - | A | 52-326 | [»] | |
2LBT | NMR | - | A | 196-326 | [»] | |
2LCA | NMR | - | A | 196-326 | [»] | |
BMRBi | P9WIU5 | |||||
SMRi | P9WIU5 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
STRINGi | 83332.Rv0899 |
Proteomic databases
PaxDbi | P9WIU5 |
Genome annotation databases
EnsemblBacteriai | CCP43647; CCP43647; Rv0899 |
GeneIDi | 23492977 885286 |
KEGGi | mtu:Rv0899 mtv:RVBD_0899 |
Organism-specific databases
TubercuListi | Rv0899 |
Phylogenomic databases
eggNOGi | COG2885, Bacteria |
OMAi | MKGEVHN |
Family and domain databases
CDDi | cd07185, OmpA_C-like, 1 hit |
Gene3Di | 3.30.1330.60, 1 hit |
InterProi | View protein in InterPro IPR007055, BON_dom IPR006664, OMP_bac IPR006665, OmpA-like IPR006690, OMPA-like_CS IPR036737, OmpA-like_sf |
Pfami | View protein in Pfam PF04972, BON, 1 hit PF00691, OmpA, 1 hit |
PRINTSi | PR01021, OMPADOMAIN |
SUPFAMi | SSF103088, SSF103088, 1 hit |
PROSITEi | View protein in PROSITE PS01068, OMPA_1, 1 hit PS51123, OMPA_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ARFA_MYCTU | |
Accessioni | P9WIU5Primary (citable) accession number: P9WIU5 Secondary accession number(s): L0T818, P65593, Q10557 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 16, 2014 |
Last sequence update: | April 16, 2014 | |
Last modified: | December 2, 2020 | |
This is version 33 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families