UniProtKB - P9WIQ1 (GLF_MYCTU)
Protein
UDP-galactopyranose mutase
Gene
glf
Organism
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Status
Functioni
Catalyzes the interconversion through a 2-keto intermediate of uridine diphosphogalactopyranose (UDP-GalP) into uridine diphosphogalactofuranose (UDP-GalF) which is a key building block for cell wall construction in Mycobacterium tuberculosis.1 Publication
Catalytic activityi
- EC:5.4.99.9
Cofactori
FAD1 Publication
: cell wall polysaccharide biosynthesis Pathwayi
This protein is involved in the pathway cell wall polysaccharide biosynthesis, which is part of Cell wall biogenesis.View all proteins of this organism that are known to be involved in the pathway cell wall polysaccharide biosynthesis and in Cell wall biogenesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 18 | FAD; via amide nitrogen1 Publication | 1 | |
Binding sitei | 38 | FAD1 Publication | 1 | |
Binding sitei | 46 | FAD; via amide nitrogen1 Publication | 1 | |
Binding sitei | 66 | FAD; via amide nitrogen and carbonyl oxygen1 Publication | 1 | |
Binding sitei | 157 | UDP-GalPBy similarity | 1 | |
Binding sitei | 162 | UDP-GalPBy similarity | 1 | |
Binding sitei | 166 | UDP-GalPBy similarity | 1 | |
Binding sitei | 191 | UDP-GalPBy similarity | 1 | |
Binding sitei | 282 | UDP-GalPBy similarity | 1 | |
Binding sitei | 292 | UDP-GalPBy similarity | 1 | |
Binding sitei | 328 | UDP-GalPBy similarity | 1 | |
Binding sitei | 360 | FAD1 Publication | 1 | |
Binding sitei | 366 | UDP-GalPBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 224 – 225 | FAD1 Publication | 2 | |
Nucleotide bindingi | 367 – 369 | FAD1 Publication | 3 |
GO - Molecular functioni
- flavin adenine dinucleotide binding Source: GO_Central
- UDP-galactopyranose mutase activity Source: MTBBASE
GO - Biological processi
- Actinobacterium-type cell wall biogenesis Source: MTBBASE
- capsule polysaccharide biosynthetic process Source: UniProtKB-UniPathway
- cell wall organization Source: UniProtKB-KW
Keywordsi
Molecular function | Isomerase |
Biological process | Cell wall biogenesis/degradation |
Ligand | FAD, Flavoprotein |
Enzyme and pathway databases
BioCyci | MetaCyc:G185E-8105-MONOMER MTBH37RV:G185E-8105-MONOMER |
UniPathwayi | UPA00963 |
Names & Taxonomyi
Protein namesi | Recommended name: UDP-galactopyranose mutase (EC:5.4.99.9)Short name: UGM Alternative name(s): UDP-GALP mutase Uridine 5-diphosphate galactopyranose mutase |
Gene namesi | Name:glf Ordered Locus Names:Rv3809c |
Organismi | Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) |
Taxonomic identifieri | 83332 [NCBI] |
Taxonomic lineagei | Bacteria › Actinobacteria › Corynebacteriales › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex › |
Proteomesi |
|
Organism-specific databases
TubercuListi | Rv3809c |
Subcellular locationi
GO - Cellular componenti
- cell wall Source: MTBBASE
- cytosol Source: GO_Central
- plasma membrane Source: MTBBASE
Pathology & Biotechi
Chemistry databases
ChEMBLi | CHEMBL4450 |
DrugBanki | DB03709 Bicine DB03147 Flavin adenine dinucleotide |
DrugCentrali | P9WIQ1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000420735 | 1 – 399 | UDP-galactopyranose mutaseAdd BLAST | 399 |
Proteomic databases
PaxDbi | P9WIQ1 |
PRIDEi | P9WIQ1 |
Interactioni
Subunit structurei
Homotetramer.
1 PublicationProtein-protein interaction databases
STRINGi | 83332.Rv3809c |
Chemistry databases
BindingDBi | P9WIQ1 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P9WIQ1 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Sequence similaritiesi
Belongs to the UDP-galactopyranose/dTDP-fucopyranose mutase family.Curated
Phylogenomic databases
eggNOGi | ENOG4105D5D Bacteria COG0562 LUCA |
KOi | K01854 |
OMAi | INVHKYG |
PhylomeDBi | P9WIQ1 |
Family and domain databases
InterProi | View protein in InterPro IPR004379 UDP-GALP_mutase IPR015899 UDP-GalPyranose_mutase_C |
Pfami | View protein in Pfam PF03275 GLF, 1 hit |
TIGRFAMsi | TIGR00031 UDP-GALP_mutase, 1 hit |
i Sequence
Sequence statusi: Complete.
P9WIQ1-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MQPMTARFDL FVVGSGFFGL TIAERVATQL DKRVLVLERR PHIGGNAYSE
60 70 80 90 100
AEPQTGIEVH KYGAHLFHTS NKRVWDYVRQ FTDFTDYRHR VFAMHNGQAY
110 120 130 140 150
QFPMGLGLVS QFFGKYFTPE QARQLIAEQA AEIDTADAQN LEEKAISLIG
160 170 180 190 200
RPLYEAFVKG YTAKQWQTDP KELPAANITR LPVRYTFDNR YFSDTYEGLP
210 220 230 240 250
TDGYTAWLQN MAADHRIEVR LNTDWFDVRG QLRPGSPAAP VVYTGPLDRY
260 270 280 290 300
FDYAEGRLGW RTLDFEVEVL PIGDFQGTAV MNYNDLDVPY TRIHEFRHFH
310 320 330 340 350
PERDYPTDKT VIMREYSRFA EDDDEPYYPI NTEADRALLA TYRARAKSET
360 370 380 390
ASSKVLFGGR LGTYQYLDMH MAIASALNMY DNVLAPHLRD GVPLLQDGA
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U96128 Genomic DNA Translation: AAC46190.1 AF026540 Genomic DNA Translation: AAC69358.1 AL123456 Genomic DNA Translation: CCP46638.1 |
PIRi | E70888 |
RefSeqi | NP_218326.1, NC_000962.3 WP_003420798.1, NZ_NVQJ01000022.1 |
Genome annotation databases
EnsemblBacteriai | CCP46638; CCP46638; Rv3809c |
GeneIDi | 886142 |
KEGGi | mtu:Rv3809c mtv:RVBD_3809c |
PATRICi | fig|83332.111.peg.4234 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U96128 Genomic DNA Translation: AAC46190.1 AF026540 Genomic DNA Translation: AAC69358.1 AL123456 Genomic DNA Translation: CCP46638.1 |
PIRi | E70888 |
RefSeqi | NP_218326.1, NC_000962.3 WP_003420798.1, NZ_NVQJ01000022.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1V0J | X-ray | 2.25 | A/B/C/D | 1-399 | [»] | |
4RPG | X-ray | 2.40 | A/B/C | 1-399 | [»] | |
4RPH | X-ray | 2.60 | A/B/C | 1-399 | [»] | |
4RPJ | X-ray | 2.50 | A/B/C | 1-399 | [»] | |
4RPK | X-ray | 2.55 | A/B/C | 1-399 | [»] | |
4RPL | X-ray | 2.25 | A/B/C | 1-399 | [»] | |
SMRi | P9WIQ1 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
STRINGi | 83332.Rv3809c |
Chemistry databases
BindingDBi | P9WIQ1 |
ChEMBLi | CHEMBL4450 |
DrugBanki | DB03709 Bicine DB03147 Flavin adenine dinucleotide |
DrugCentrali | P9WIQ1 |
Proteomic databases
PaxDbi | P9WIQ1 |
PRIDEi | P9WIQ1 |
Genome annotation databases
EnsemblBacteriai | CCP46638; CCP46638; Rv3809c |
GeneIDi | 886142 |
KEGGi | mtu:Rv3809c mtv:RVBD_3809c |
PATRICi | fig|83332.111.peg.4234 |
Organism-specific databases
TubercuListi | Rv3809c |
Phylogenomic databases
eggNOGi | ENOG4105D5D Bacteria COG0562 LUCA |
KOi | K01854 |
OMAi | INVHKYG |
PhylomeDBi | P9WIQ1 |
Enzyme and pathway databases
UniPathwayi | UPA00963 |
BioCyci | MetaCyc:G185E-8105-MONOMER MTBH37RV:G185E-8105-MONOMER |
Miscellaneous databases
PROi | PR:P9WIQ1 |
Family and domain databases
InterProi | View protein in InterPro IPR004379 UDP-GALP_mutase IPR015899 UDP-GalPyranose_mutase_C |
Pfami | View protein in Pfam PF03275 GLF, 1 hit |
TIGRFAMsi | TIGR00031 UDP-GALP_mutase, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | GLF_MYCTU | |
Accessioni | P9WIQ1Primary (citable) accession number: P9WIQ1 Secondary accession number(s): F2GDG8 Q7D4U3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 16, 2014 |
Last sequence update: | April 16, 2014 | |
Last modified: | September 18, 2019 | |
This is version 32 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Complete proteome, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families - Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh
Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh: entries and gene names - PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references