UniProtKB - P9WIB5 (PHLA_MYCTU)
Protein
Phospholipase C A
Gene
plcA
Organism
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Status
Functioni
Involved in virulence (PubMed:20736081). Induces cytotoxic effects on mouse macrophage cell lines, via direct or indirect enzymatic hydrolysis of cell membrane phospholipids (PubMed:20736081). Hydrolyzes phosphatidylcholine and sphingomyelin (PubMed:8757862, PubMed:20736081). Does not have hemolytic activity (PubMed:20736081).2 Publications
Miscellaneous
Polymorphism was discovered in the phospholipase plcA/B/C region. Some strains seem to lack both plcA and plcB genes, while others lack only plcB.1 Publication
An IS6110 insertion element has been found in strain LCDC-194, interrupting the plcA gene.1 Publication
Catalytic activityi
- a 1,2-diacyl-sn-glycero-3-phosphocholine + H2O = a 1,2-diacyl-sn-glycerol + H+ + phosphocholine2 PublicationsEC:3.1.4.32 PublicationsThis reaction proceeds in the forward2 Publications direction.
- This reaction proceeds in the forward1 Publication direction.
- 1,2-dihexadecanoyl-sn-glycero-3-phosphocholine + H2O = 1,2-dihexadecanoyl-sn-glycerol + H+ + phosphocholine1 PublicationThis reaction proceeds in the forward1 Publication direction.
pH dependencei
Optimum pH is 7.5.1 Publication
Temperature dependencei
Optimum temperature is 37 degrees Celsius.1 Publication
GO - Molecular functioni
- phosphatidylcholine phospholipase C activity Source: MTBBASE
- phospholipase C activity Source: MTBBASE
GO - Biological processi
- disruption by symbiont of host cellular component Source: MTBBASE
- pathogenesis Source: MTBBASE
Keywordsi
Molecular function | Hydrolase |
Biological process | Virulence |
Chemistry databases
SwissLipidsi | SLP:000001395 |
Names & Taxonomyi
Protein namesi | Recommended name: Phospholipase C ACurated (EC:3.1.4.32 Publications)Short name: PLC-A1 Publication Alternative name(s): MTP40 antigen Mycobacterial phospholipase C A1 Publication |
Gene namesi | Name:plcA1 Publication Synonyms:mpcA1 Publication, mtp401 Publication Ordered Locus Names:Rv2351c ORF Names:MTCY98.20c |
Organismi | Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) |
Taxonomic identifieri | 83332 [NCBI] |
Taxonomic lineagei | Bacteria › Actinobacteria › Corynebacteriales › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex › |
Proteomesi |
|
Organism-specific databases
TubercuListi | Rv2351c |
Subcellular locationi
- cell wall 1 Publication Note: Remains associated with the cell.3 Publications
GO - Cellular componenti
- cell wall Source: UniProtKB-SubCell
- extracellular region Source: UniProtKB-KW
Keywords - Cellular componenti
Cell wall, SecretedPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 38 | Tat-type signalPROSITE-ProRule annotationAdd BLAST | 38 | |
ChainiPRO_0000023942 | 39 – 520 | Phospholipase C AAdd BLAST | 482 |
Post-translational modificationi
Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.PROSITE-ProRule annotation
Proteomic databases
PaxDbi | P9WIB5 |
Expressioni
Inductioni
Family & Domainsi
Sequence similaritiesi
Belongs to the bacterial phospholipase C family.Curated
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | COG3511, Bacteria |
OMAi | PVFNAMA |
Family and domain databases
Gene3Di | 3.40.720.10, 2 hits |
InterProi | View protein in InterPro IPR017850, Alkaline_phosphatase_core_sf IPR007312, Phosphoesterase IPR006311, TAT_signal |
PANTHERi | PTHR31956, PTHR31956, 1 hit |
Pfami | View protein in Pfam PF04185, Phosphoesterase, 1 hit |
PROSITEi | View protein in PROSITE PS51318, TAT, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P9WIB5-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSASPLLGMS RREFLTKLTG AGAAAFLMDW AAPVIEKAYG AGPCPGHLTD
60 70 80 90 100
IEHIVLLMQE NRSFDHYFGT LSSTNGFNAA SPAFQQMGWN PMTQALDPAG
110 120 130 140 150
VTIPFRLDTT RGPFLDGECV NDPEHQWVGM HLAWNGGAND NWLPAQATTR
160 170 180 190 200
AGPYVPLTMG YYTRQDIPIH YLLADTFTIC DGYHCSLLTG TLPNRLYWLS
210 220 230 240 250
ANIDPAGTDG GPQLVEPGFL PLQQFSWRIM PENLEDAGVS WKVYQNKGLG
260 270 280 290 300
RFINTPISNN GLVQAFRQAA DPRSNLARYG IAPTYPGDFA ADVRANRLPK
310 320 330 340 350
VSWLVPNILQ SEHPALPVAL GAVSMVTALR ILLSNPAVWE KTALIVSYDE
360 370 380 390 400
NGGFFDHVTP PTAPPGTPGE FVTVPNIDAV PGSGGIRGPL GLGFRVPCIV
410 420 430 440 450
ISPYSRGPLM VSDTFDHTSQ LKLIRARFGV PVPNMTAWRD GVVGDMTSAF
460 470 480 490 500
NFATPPNSTR PNLSHPLLGA LPKLPQCIPN VVLGTTDGAL PSIPYRVPYP
510 520
QVMPTQETTP VRGTPSGLCS
Sequence cautioni
The sequence AAA63288 differs from that shown. Reason: Frameshift.Curated
The sequence AAB59164 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAC60465 differs from that shown. Reason: Frameshift.Curated
The sequence CCP45139 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 327 | T → N (PubMed:1909999).Curated | 1 | |
Sequence conflicti | 369 – 370 | GE → AQ (PubMed:1909999).Curated | 2 | |
Sequence conflicti | 406 – 407 | RG → PR (PubMed:1909999).Curated | 2 | |
Sequence conflicti | 516 – 520 | SGLCS → AGCAAEISR (PubMed:1909999).Curated | 5 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L11868 Genomic DNA Translation: AAB59164.1 Different initiation. L11868 Genomic DNA Translation: AAB59165.1 Sequence problems. U49511 Genomic DNA Translation: AAC18943.1 AL123456 Genomic DNA Translation: CCP45139.1 Different initiation. M57952 Genomic DNA Translation: AAA63288.1 Frameshift. S69737 Genomic DNA Translation: AAC60465.2 Frameshift. |
PIRi | H70662 |
RefSeqi | NP_216867.1, NC_000962.3 |
Genome annotation databases
EnsemblBacteriai | CCP45139; CCP45139; Rv2351c |
GeneIDi | 885995 |
KEGGi | mtu:Rv2351c |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L11868 Genomic DNA Translation: AAB59164.1 Different initiation. L11868 Genomic DNA Translation: AAB59165.1 Sequence problems. U49511 Genomic DNA Translation: AAC18943.1 AL123456 Genomic DNA Translation: CCP45139.1 Different initiation. M57952 Genomic DNA Translation: AAA63288.1 Frameshift. S69737 Genomic DNA Translation: AAC60465.2 Frameshift. |
PIRi | H70662 |
RefSeqi | NP_216867.1, NC_000962.3 |
3D structure databases
SMRi | P9WIB5 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 83332.Rv2351c |
Chemistry databases
SwissLipidsi | SLP:000001395 |
Proteomic databases
PaxDbi | P9WIB5 |
Genome annotation databases
EnsemblBacteriai | CCP45139; CCP45139; Rv2351c |
GeneIDi | 885995 |
KEGGi | mtu:Rv2351c |
Organism-specific databases
TubercuListi | Rv2351c |
Phylogenomic databases
eggNOGi | COG3511, Bacteria |
OMAi | PVFNAMA |
Miscellaneous databases
PHI-basei | PHI:5288 |
Family and domain databases
Gene3Di | 3.40.720.10, 2 hits |
InterProi | View protein in InterPro IPR017850, Alkaline_phosphatase_core_sf IPR007312, Phosphoesterase IPR006311, TAT_signal |
PANTHERi | PTHR31956, PTHR31956, 1 hit |
Pfami | View protein in Pfam PF04185, Phosphoesterase, 1 hit |
PROSITEi | View protein in PROSITE PS51318, TAT, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PHLA_MYCTU | |
Accessioni | P9WIB5Primary (citable) accession number: P9WIB5 Secondary accession number(s): L0T9D3 Q53408 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 16, 2014 |
Last sequence update: | April 16, 2014 | |
Last modified: | December 2, 2020 | |
This is version 39 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families