Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Serine/threonine-protein kinase PknB

Gene

pknB

Organism
Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Protein kinase that regulates many aspects of mycobacterial physiology. Is a key component of a signal transduction pathway that regulates cell growth, cell shape and cell division via phosphorylation of target proteins.By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei40ATPPROSITE-ProRule annotation1
Active sitei138Proton acceptorPROSITE-ProRule annotation1
Metal bindingi143MagnesiumBy similarity1
Metal bindingi156MagnesiumBy similarity1
Binding sitei156ATPPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi17 – 25ATPPROSITE-ProRule annotation9
Nucleotide bindingi93 – 95ATPPROSITE-ProRule annotation3
Nucleotide bindingi140 – 143ATPPROSITE-ProRule annotation4

GO - Molecular functioni

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase PknB (EC:2.7.11.1)
Gene namesi
Name:pknB
Ordered Locus Names:MT0017
OrganismiMycobacterium tuberculosis (strain CDC 1551 / Oshkosh)
Taxonomic identifieri83331 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
Proteomesi
  • UP000001020 Componenti: Chromosome

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 332CytoplasmicSequence analysisAdd BLAST332
Transmembranei333 – 353HelicalSequence analysisAdd BLAST21
Topological domaini354 – 626ExtracellularSequence analysisAdd BLAST273

GO - Cellular componenti

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004280531 – 626Serine/threonine-protein kinase PknBAdd BLAST626

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei166Phosphoserine; by autocatalysisBy similarity1
Modified residuei169Phosphoserine; by autocatalysisBy similarity1
Modified residuei171Phosphothreonine; by autocatalysisBy similarity1
Modified residuei173Phosphothreonine; by autocatalysisBy similarity1
Modified residuei294Phosphothreonine; by autocatalysisBy similarity1
Modified residuei295Phosphoserine; by autocatalysisBy similarity1
Modified residuei309Phosphothreonine; by autocatalysisBy similarity1

Post-translational modificationi

Autophosphorylated. Dephosphorylated by PstP (By similarity).By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiP9WI80

Interactioni

Subunit structurei

Homodimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliP9WI80
SMRiP9WI80
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini11 – 274Protein kinasePROSITE-ProRule annotationAdd BLAST264
Domaini356 – 422PASTA 1PROSITE-ProRule annotationAdd BLAST67
Domaini423 – 490PASTA 2PROSITE-ProRule annotationAdd BLAST68
Domaini491 – 557PASTA 3PROSITE-ProRule annotationAdd BLAST67
Domaini558 – 626PASTA 4PROSITE-ProRule annotationAdd BLAST69

Domaini

The PASTA domains interact with peptidoglycans and are required for PknB localization.By similarity

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

KOiK12132
OrthoDBiPOG091H0264

Family and domain databases

InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR005543 PASTA_dom
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR008271 Ser/Thr_kinase_AS
PfamiView protein in Pfam
PF03793 PASTA, 4 hits
PF00069 Pkinase, 1 hit
SMARTiView protein in SMART
SM00740 PASTA, 4 hits
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS51178 PASTA, 4 hits
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit

Sequencei

Sequence statusi: Complete.

P9WI80-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MTTPSHLSDR YELGEILGFG GMSEVHLARD LRLHRDVAVK VLRADLARDP
60 70 80 90 100
SFYLRFRREA QNAAALNHPA IVAVYDTGEA ETPAGPLPYI VMEYVDGVTL
110 120 130 140 150
RDIVHTEGPM TPKRAIEVIA DACQALNFSH QNGIIHRDVK PANIMISATN
160 170 180 190 200
AVKVMDFGIA RAIADSGNSV TQTAAVIGTA QYLSPEQARG DSVDARSDVY
210 220 230 240 250
SLGCVLYEVL TGEPPFTGDS PVSVAYQHVR EDPIPPSARH EGLSADLDAV
260 270 280 290 300
VLKALAKNPE NRYQTAAEMR ADLVRVHNGE PPEAPKVLTD AERTSLLSSA
310 320 330 340 350
AGNLSGPRTD PLPRQDLDDT DRDRSIGSVG RWVAVVAVLA VLTVVVTIAI
360 370 380 390 400
NTFGGITRDV QVPDVRGQSS ADAIATLQNR GFKIRTLQKP DSTIPPDHVI
410 420 430 440 450
GTDPAANTSV SAGDEITVNV STGPEQREIP DVSTLTYAEA VKKLTAAGFG
460 470 480 490 500
RFKQANSPST PELVGKVIGT NPPANQTSAI TNVVIIIVGS GPATKDIPDV
510 520 530 540 550
AGQTVDVAQK NLNVYGFTKF SQASVDSPRP AGEVTGTNPP AGTTVPVDSV
560 570 580 590 600
IELQVSKGNQ FVMPDLSGMF WVDAEPRLRA LGWTGMLDKG ADVDAGGSQH
610 620
NRVVYQNPPA GTGVNRDGII TLRFGQ
Length:626
Mass (Da):66,510
Last modified:April 16, 2014 - v1
Checksum:i6C27EEBE9D5A453B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000516 Genomic DNA Translation: AAK44239.1
PIRiD70699
RefSeqiWP_003400356.1, NZ_KK341227.1

Genome annotation databases

EnsemblBacteriaiAAK44239; AAK44239; MT0017
KEGGimtc:MT0017
PATRICifig|83331.31.peg.18

Similar proteinsi

Entry informationi

Entry nameiPKNB_MYCTO
AccessioniPrimary (citable) accession number: P9WI80
Secondary accession number(s): L0T5F6, P0A5S4, P71584
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 16, 2014
Last sequence update: April 16, 2014
Last modified: May 23, 2018
This is version 26 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again