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Protein

Protein RecA

Gene

recA

Organism
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Can catalyze the hydrolysis of ATP in the presence of single-stranded DNA, the ATP-dependent uptake of single-stranded DNA by duplex DNA, and the ATP-dependent hybridization of homologous single-stranded DNAs. It interacts with LexA causing its activation and leading to its autocatalytic cleavage.
PI-MtuI is an endonuclease.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi67 – 74ATPBy similarity8

GO - Molecular functioni

  • ATPase activity Source: MTBBASE
  • ATP binding Source: MTBBASE
  • DNA-dependent ATPase activity Source: MTBBASE
  • endodeoxyribonuclease activity Source: MTBBASE
  • magnesium ion binding Source: MTBBASE
  • manganese ion binding Source: MTBBASE
  • recombinase activity Source: MTBBASE
  • single-stranded DNA binding Source: MTBBASE

GO - Biological processi

  • cellular response to DNA damage stimulus Source: MTBBASE
  • intein-mediated protein splicing Source: InterPro
  • intron homing Source: UniProtKB-KW
  • recombinational repair Source: MTBBASE
  • response to antibiotic Source: MTBBASE
  • SOS response Source: UniProtKB-KW
  • strand invasion Source: MTBBASE
  • UV protection Source: MTBBASE

Keywordsi

Molecular functionDNA-binding, Endonuclease, Hydrolase, Nuclease
Biological processDNA damage, DNA recombination, DNA repair, Intron homing, SOS response
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Protein RecA
Alternative name(s):
Recombinase A
Cleaved into the following chain:
Alternative name(s):
Mtu RecA intein
Gene namesi
Name:recA
Ordered Locus Names:Rv2737c
ORF Names:MTV002.02c
OrganismiMycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Taxonomic identifieri83332 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
Proteomesi
  • UP000001584 Componenti: Chromosome

Organism-specific databases

TubercuListiRv2737c

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: MTBBASE

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL1741171

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000302691 – 251Protein RecA, 1st partAdd BLAST251
ChainiPRO_0000030270252 – 691Endonuclease PI-MtuIAdd BLAST440
ChainiPRO_0000030271692 – 790Protein RecA, 2nd partAdd BLAST99

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki762Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)1 Publication

Post-translational modificationi

This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation.

Keywords - PTMi

Autocatalytic cleavage, Isopeptide bond, Protein splicing, Ubl conjugation

Proteomic databases

PaxDbiP9WHJ3
PRIDEiP9WHJ3

Interactioni

Subunit structurei

Interacts with UvrD1 and UvrA.1 Publication

Protein-protein interaction databases

STRINGi83332.Rv2737c

Chemistry databases

BindingDBiP9WHJ3

Structurei

Secondary structure

1790
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi5 – 22Combined sources18
Helixi46 – 51Combined sources6
Beta strandi53 – 58Combined sources6
Beta strandi61 – 68Combined sources8
Helixi73 – 86Combined sources14
Beta strandi91 – 97Combined sources7
Helixi102 – 108Combined sources7
Helixi112 – 114Combined sources3
Beta strandi116 – 118Combined sources3
Helixi123 – 135Combined sources13
Beta strandi140 – 145Combined sources6
Helixi147 – 149Combined sources3
Helixi153 – 156Combined sources4
Helixi167 – 186Combined sources20
Beta strandi189 – 195Combined sources7
Helixi213 – 219Combined sources7
Beta strandi221 – 234Combined sources14
Beta strandi237 – 252Combined sources16
Beta strandi258 – 260Combined sources3
Turni262 – 264Combined sources3
Beta strandi267 – 269Combined sources3
Helixi270 – 275Combined sources6
Beta strandi281 – 285Combined sources5
Beta strandi287 – 289Combined sources3
Beta strandi291 – 312Combined sources22
Beta strandi317 – 320Combined sources4
Beta strandi325 – 328Combined sources4
Beta strandi331 – 334Combined sources4
Helixi335 – 337Combined sources3
Beta strandi343 – 345Combined sources3
Turni349 – 351Combined sources3

3D structure databases

ProteinModelPortaliP9WHJ3
SMRiP9WHJ3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini366 – 508DOD-type homing endonucleaseAdd BLAST143

Sequence similaritiesi

Belongs to the RecA family.Curated

Phylogenomic databases

eggNOGiCOG0468 LUCA
COG1372 LUCA
KOiK03553
OMAiFVDFTPL
PhylomeDBiP9WHJ3

Family and domain databases

CDDicd00983 recA, 1 hit
Gene3Di3.10.28.10, 1 hit
3.30.250.10, 1 hit
HAMAPiMF_00268 RecA, 1 hit
InterProiView protein in InterPro
IPR003593 AAA+_ATPase
IPR013765 DNA_recomb/repair_RecA
IPR020584 DNA_recomb/repair_RecA_CS
IPR003586 Hint_dom_C
IPR003587 Hint_dom_N
IPR036844 Hint_dom_sf
IPR027434 Homing_endonucl
IPR006142 INTEIN
IPR030934 Intein_C
IPR004042 Intein_endonuc
IPR006141 Intein_N
IPR004860 LAGLIDADG_2
IPR027417 P-loop_NTPase
IPR020588 RecA_ATP-bd
IPR023400 RecA_C
IPR020587 RecA_monomer-monomer_interface
PANTHERiPTHR22942:SF1 PTHR22942:SF1, 1 hit
PfamiView protein in Pfam
PF14528 LAGLIDADG_3, 1 hit
PF00154 RecA, 1 hit
PRINTSiPR00379 INTEIN
PR00142 RECA
SMARTiView protein in SMART
SM00382 AAA, 1 hit
SM00305 HintC, 1 hit
SM00306 HintN, 1 hit
SUPFAMiSSF51294 SSF51294, 2 hits
SSF52540 SSF52540, 1 hit
SSF54752 SSF54752, 1 hit
SSF55608 SSF55608, 1 hit
TIGRFAMsiTIGR01443 intein_Cterm, 1 hit
TIGR01445 intein_Nterm, 1 hit
TIGR02012 tigrfam_recA, 1 hit
PROSITEiView protein in PROSITE
PS50818 INTEIN_C_TER, 1 hit
PS50819 INTEIN_ENDONUCLEASE, 1 hit
PS50817 INTEIN_N_TER, 1 hit
PS00321 RECA_1, 1 hit
PS50162 RECA_2, 1 hit
PS50163 RECA_3, 2 hits

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P9WHJ3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTQTPDREKA LELAVAQIEK SYGKGSVMRL GDEARQPISV IPTGSIALDV
60 70 80 90 100
ALGIGGLPRG RVIEIYGPES SGKTTVALHA VANAQAAGGV AAFIDAEHAL
110 120 130 140 150
DPDYAKKLGV DTDSLLVSQP DTGEQALEIA DMLIRSGALD IVVIDSVAAL
160 170 180 190 200
VPRAELEGEM GDSHVGLQAR LMSQALRKMT GALNNSGTTA IFINQLRDKI
210 220 230 240 250
GVMFGSPETT TGGKALKFYA SVRMDVRRVE TLKDGTNAVG NRTRVKVVKN
260 270 280 290 300
KCLAEGTRIF DPVTGTTHRI EDVVDGRKPI HVVAAAKDGT LHARPVVSWF
310 320 330 340 350
DQGTRDVIGL RIAGGAIVWA TPDHKVLTEY GWRAAGELRK GDRVAQPRRF
360 370 380 390 400
DGFGDSAPIP ADHARLLGYL IGDGRDGWVG GKTPINFINV QRALIDDVTR
410 420 430 440 450
IAATLGCAAH PQGRISLAIA HRPGERNGVA DLCQQAGIYG KLAWEKTIPN
460 470 480 490 500
WFFEPDIAAD IVGNLLFGLF ESDGWVSREQ TGALRVGYTT TSEQLAHQIH
510 520 530 540 550
WLLLRFGVGS TVRDYDPTQK RPSIVNGRRI QSKRQVFEVR ISGMDNVTAF
560 570 580 590 600
AESVPMWGPR GAALIQAIPE ATQGRRRGSQ ATYLAAEMTD AVLNYLDERG
610 620 630 640 650
VTAQEAAAMI GVASGDPRGG MKQVLGASRL RRDRVQALAD ALDDKFLHDM
660 670 680 690 700
LAEELRYSVI REVLPTRRAR TFDLEVEELH TLVAEGVVVH NCSPPFKQAE
710 720 730 740 750
FDILYGKGIS REGSLIDMGV DQGLIRKSGA WFTYEGEQLG QGKENARNFL
760 770 780 790
VENADVADEI EKKIKEKLGI GAVVTDDPSN DGVLPAPVDF
Length:790
Mass (Da):85,389
Last modified:April 16, 2014 - v1
Checksum:iAD16340D2ED5E572
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti305R → Q in strain: Canetti and SO93. 1
Natural varianti430A → L in strain: Canetti and SO93. 1
Natural varianti434 – 435QQ → RR in strain: Canetti and SO93. 2
Natural varianti438 – 439IY → VH in strain: Canetti and SO93. 2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X58485 Genomic DNA Translation: CAA41395.1
AJ000012 Genomic DNA Translation: CAA03857.1
AJ000011 Genomic DNA Translation: CAA03856.1
AL123456 Genomic DNA Translation: CCP45535.1
PIRiS18206
RefSeqiNP_217253.1, NC_000962.3
WP_003414006.1, NZ_KK339370.1

Genome annotation databases

EnsemblBacteriaiCCP45535; CCP45535; Rv2737c
GeneIDi888371
KEGGimtu:Rv2737c

Similar proteinsi

Entry informationi

Entry nameiRECA_MYCTU
AccessioniPrimary (citable) accession number: P9WHJ3
Secondary accession number(s): L0TAH5
, O34519, P0A5U4, P26345
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 16, 2014
Last sequence update: April 16, 2014
Last modified: July 18, 2018
This is version 37 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Intein-containing proteins
    List of intein-containing protein entries
  2. Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh
    Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh: entries and gene names
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

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