UniProtKB - P9WHH3 (MTR_MYCTU)
Protein
Mycothione reductase
Gene
mtr
Organism
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Status
Functioni
Catalyzes the NAD(P)H-dependent reduction of mycothione (the oxidized disulfide form of mycothiol) to mycothiol.1 Publication
Catalytic activityi
Cofactori
FAD1 PublicationNote: Binds 1 FAD per subunit.1 Publication
Kineticsi
- KM=7.7 µM for NADPH2 Publications
- KM=43 µM for NADH2 Publications
- KM=70 µM for mycothione2 Publications
pH dependencei
Optimum pH is 7-9.2 Publications
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 444 | Proton acceptor | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 31 – 39 | FADBy similarity | 9 |
GO - Molecular functioni
- dihydrolipoyl dehydrogenase activity Source: GO_Central
- flavin adenine dinucleotide binding Source: MTBBASE
- mycothione reductase activity Source: MTBBASE
- NADPH binding Source: MTBBASE
GO - Biological processi
- cell redox homeostasis Source: InterPro
- mycothiol metabolic process Source: Reactome
- oxidation-reduction process Source: MTBBASE
Keywordsi
Molecular function | Oxidoreductase |
Ligand | FAD, Flavoprotein, NAD, NADP |
Enzyme and pathway databases
BioCyci | MetaCyc:G185E-7106-MONOMER |
Reactomei | R-MTU-870331, Mycothiol metabolism |
SABIO-RKi | P9WHH3 |
Names & Taxonomyi
Protein namesi | Recommended name: Mycothione reductase (EC:1.8.1.15)Alternative name(s): Mycothiol-disulfide reductase NADPH-dependent mycothione reductase |
Gene namesi | Name:mtr Synonyms:gorA Ordered Locus Names:Rv2855 |
Organismi | Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) |
Taxonomic identifieri | 83332 [NCBI] |
Taxonomic lineagei | Bacteria › Actinobacteria › Corynebacteriales › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex › |
Proteomesi |
|
Organism-specific databases
TubercuListi | Rv2855 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000399830 | 1 – 459 | Mycothione reductaseAdd BLAST | 459 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 39 ↔ 44 | Redox-active |
Keywords - PTMi
Disulfide bondProteomic databases
PaxDbi | P9WHH3 |
Interactioni
Subunit structurei
Homodimer.
1 PublicationProtein-protein interaction databases
STRINGi | 83332.Rv2855 |
Family & Domainsi
Sequence similaritiesi
Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.Curated
Keywords - Domaini
Redox-active centerPhylogenomic databases
eggNOGi | COG1249, Bacteria |
OMAi | KCAIIEA |
PhylomeDBi | P9WHH3 |
Family and domain databases
Gene3Di | 3.30.390.30, 1 hit 3.50.50.60, 2 hits |
InterProi | View protein in InterPro IPR036188, FAD/NAD-bd_sf IPR023753, FAD/NAD-binding_dom IPR016156, FAD/NAD-linked_Rdtase_dimer_sf IPR017817, Mycothione_reductase IPR001100, Pyr_nuc-diS_OxRdtase IPR004099, Pyr_nucl-diS_OxRdtase_dimer IPR012999, Pyr_OxRdtase_I_AS |
Pfami | View protein in Pfam PF07992, Pyr_redox_2, 1 hit PF02852, Pyr_redox_dim, 1 hit |
PIRSFi | PIRSF000350, Mercury_reductase_MerA, 1 hit |
SUPFAMi | SSF51905, SSF51905, 1 hit SSF55424, SSF55424, 1 hit |
TIGRFAMsi | TIGR03452, mycothione_red, 1 hit |
PROSITEi | View protein in PROSITE PS00076, PYRIDINE_REDOX_1, 1 hit |
i Sequence
Sequence statusi: Complete.
P9WHH3-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
METYDIAIIG TGSGNSILDE RYASKRAAIC EQGTFGGTCL NVGCIPTKMF
60 70 80 90 100
VYAAEVAKTI RGASRYGIDA HIDRVRWDDV VSRVFGRIDP IALSGEDYRR
110 120 130 140 150
CAPNIDVYRT HTRFGPVQAD GRYLLRTDAG EEFTAEQVVI AAGSRPVIPP
160 170 180 190 200
AILASGVDYH TSDTVMRIAE LPEHIVIVGS GFIAAEFAHV FSALGVRVTL
210 220 230 240 250
VIRGSCLLRH CDDTICERFT RIASTKWELR THRNVVDGQQ RGSGVALRLD
260 270 280 290 300
DGCTINADLL LVATGRVSNA DLLDAEQAGV DVEDGRVIVD EYQRTSARGV
310 320 330 340 350
FALGDVSSPY LLKHVANHEA RVVQHNLLCD WEDTQSMIVT DHRYVPAAVF
360 370 380 390 400
TDPQIAAVGL TENQAVAKGL DISVKIQDYG DVAYGWAMED TSGIVKLITE
410 420 430 440 450
RGSGRLLGAH IMGYQASSLI QPLIQAMSFG LTAAEMARGQ YWIHPALPEV
VENALLGLR
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF002193 Genomic DNA Translation: AAB63369.1 AL123456 Genomic DNA Translation: CCP45656.1 |
PIRi | B70590 |
RefSeqi | WP_003414557.1, NZ_NVQJ01000006.1 YP_177910.1, NC_000962.3 |
Genome annotation databases
EnsemblBacteriai | CCP45656; CCP45656; Rv2855 |
GeneIDi | 887773 |
KEGGi | mtu:Rv2855 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF002193 Genomic DNA Translation: AAB63369.1 AL123456 Genomic DNA Translation: CCP45656.1 |
PIRi | B70590 |
RefSeqi | WP_003414557.1, NZ_NVQJ01000006.1 YP_177910.1, NC_000962.3 |
3D structure databases
SMRi | P9WHH3 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 83332.Rv2855 |
Chemistry databases
ChEMBLi | CHEMBL1075170 |
Proteomic databases
PaxDbi | P9WHH3 |
Genome annotation databases
EnsemblBacteriai | CCP45656; CCP45656; Rv2855 |
GeneIDi | 887773 |
KEGGi | mtu:Rv2855 |
Organism-specific databases
TubercuListi | Rv2855 |
Phylogenomic databases
eggNOGi | COG1249, Bacteria |
OMAi | KCAIIEA |
PhylomeDBi | P9WHH3 |
Enzyme and pathway databases
BioCyci | MetaCyc:G185E-7106-MONOMER |
Reactomei | R-MTU-870331, Mycothiol metabolism |
SABIO-RKi | P9WHH3 |
Miscellaneous databases
PROi | PR:P9WHH3 |
Family and domain databases
Gene3Di | 3.30.390.30, 1 hit 3.50.50.60, 2 hits |
InterProi | View protein in InterPro IPR036188, FAD/NAD-bd_sf IPR023753, FAD/NAD-binding_dom IPR016156, FAD/NAD-linked_Rdtase_dimer_sf IPR017817, Mycothione_reductase IPR001100, Pyr_nuc-diS_OxRdtase IPR004099, Pyr_nucl-diS_OxRdtase_dimer IPR012999, Pyr_OxRdtase_I_AS |
Pfami | View protein in Pfam PF07992, Pyr_redox_2, 1 hit PF02852, Pyr_redox_dim, 1 hit |
PIRSFi | PIRSF000350, Mercury_reductase_MerA, 1 hit |
SUPFAMi | SSF51905, SSF51905, 1 hit SSF55424, SSF55424, 1 hit |
TIGRFAMsi | TIGR03452, mycothione_red, 1 hit |
PROSITEi | View protein in PROSITE PS00076, PYRIDINE_REDOX_1, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MTR_MYCTU | |
Accessioni | P9WHH3Primary (citable) accession number: P9WHH3 Secondary accession number(s): L0TB27 Q7D6G4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 16, 2014 |
Last sequence update: | April 16, 2014 | |
Last modified: | February 10, 2021 | |
This is version 49 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families