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Entry version 28 (08 May 2019)
Sequence version 1 (16 Apr 2014)
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Protein

Transcription termination factor Rho

Gene

rho

Organism
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Status
Reviewed-Annotation score:

Annotation score:4 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the ‘protein existence’ evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Facilitates transcription termination by a mechanism that involves Rho binding to the nascent RNA, activation of Rho's RNA-dependent ATPase activity, and release of the mRNA from the DNA template. Shows poor RNA-dependent ATP hydrolysis and inefficient DNA-RNA unwinding activities, but exhibits robust and fast transcription termination, which suggests that the transcription termination function of M.tuberculosis Rho is not correlated with its helicase/translocase activities and that these functions may not be important for its RNA release process.UniRule annotation1 Publication

Miscellaneous

Was identified as a high-confidence drug target.

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes regulatory mechanisms for enzymes, transporters or microbial transcription factors, and reports the components which regulate (by activation or inhibition) the reaction.<p><a href='/help/activity_regulation' target='_top'>More...</a></p>Activity regulationi

The antibiotic bicyclomycin inhibits ATPase activity but does not affect termination function.1 Publication

<p>This subsection of the ‘Function’ section describes biophysical and chemical properties, such as maximal absorption, kinetic parameters, pH dependence, redox potentials and temperature dependence.<p><a href='/help/biophysicochemical_properties' target='_top'>More...</a></p>Kineticsi

  1. KM=70 µM for ATP1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes the interaction between a single amino acid and another chemical entity. Priority is given to the annotation of physiological ligands.<p><a href='/help/binding' target='_top'>More...</a></p>Binding sitei387ATPUniRule annotation1

    Regions

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes a region in the protein which binds nucleotide phosphates. It always involves more than one amino acid and includes all residues involved in nucleotide-binding.<p><a href='/help/np_bind' target='_top'>More...</a></p>Nucleotide bindingi344 – 349ATPUniRule annotation6
    Nucleotide bindingi356 – 361ATPUniRule annotation6

    <p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

    GO - Biological processi

    <p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

    Molecular functionHelicase, Hydrolase, RNA-binding
    Biological processTranscription, Transcription regulation, Transcription termination
    LigandATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyc Collection of Pathway/Genome Databases

    More...
    BioCyci
    MTBH37RV:G185E-5471-MONOMER

    <p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

    <p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
    Recommended name:
    Transcription termination factor RhoUniRule annotation (EC:3.6.4.-UniRule annotation)
    Alternative name(s):
    ATP-dependent helicase RhoUniRule annotation
    <p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: ‘Name’, ‘Synonyms’, ‘Ordered locus names’ and ‘ORF names’.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
    Name:rhoUniRule annotation
    Ordered Locus Names:Rv1297
    ORF Names:MTCY373.17
    <p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiMycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
    <p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the ‘taxonomic identifier’ or ‘taxid’.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri83332 [NCBI]
    <p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
    <p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
    • UP000001584 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes_manual">proteome</a> can consist of several components. <br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome

    Organism-specific databases

    Mycobacterium tuberculosis strain H37Rv genome database

    More...
    TubercuListi
    Rv1297

    <p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

    GO - Cellular componenti

    <p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the ‘PTM / Processing’ section describes the extent of a polypeptide chain in the mature protein following processing.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00001889701 – 602Transcription termination factor RhoAdd BLAST602

    Proteomic databases

    PaxDb, a database of protein abundance averages across all three domains of life

    More...
    PaxDbi
    P9WHF3

    PRoteomics IDEntifications database

    More...
    PRIDEi
    P9WHF3

    <p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

    <p>This subsection of the <a href="http://www.uniprot.org/help/interaction_section">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

    Homohexamer. The homohexamer assembles into an open ring structure. Does not bind NusG.UniRule annotation1 Publication

    Protein-protein interaction databases

    STRING: functional protein association networks

    More...
    STRINGi
    83332.Rv1297

    <p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

    3D structure databases

    SWISS-MODEL Repository - a database of annotated 3D protein structure models

    More...
    SMRi
    P9WHF3

    Database of comparative protein structure models

    More...
    ModBasei
    Search...

    MobiDB: a database of protein disorder and mobility annotations

    More...
    MobiDBi
    Search...

    <p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the <a href="http://www.uniprot.org/help/family_and_domains_section">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini223 – 301Rho RNA-BDPROSITE-ProRule annotationAdd BLAST79

    <p>This subsection of the ‘Family and domains’ section provides general information on the biological role of a domain. The term ‘domain’ is intended here in its wide acceptation, it may be a structural domain, a transmembrane region or a functional domain. Several domains are described in this subsection.<p><a href='/help/domain_cc' target='_top'>More...</a></p>Domaini

    Contains an extra N-terminal domain, a S1-like RNA-binding domain and a conserved P-loop NTPase domain.1 Publication

    <p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

    Belongs to the Rho family.UniRule annotation

    Phylogenomic databases

    evolutionary genealogy of genes: Non-supervised Orthologous Groups

    More...
    eggNOGi
    ENOG4105C4P Bacteria
    COG1158 LUCA

    KEGG Orthology (KO)

    More...
    KOi
    K03628

    Identification of Orthologs from Complete Genome Data

    More...
    OMAi
    PADDHTT

    Database for complete collections of gene phylogenies

    More...
    PhylomeDBi
    P9WHF3

    Family and domain databases

    Conserved Domains Database

    More...
    CDDi
    cd01128 rho_factor, 1 hit

    HAMAP database of protein families

    More...
    HAMAPi
    MF_01884 Rho, 1 hit

    Integrated resource of protein families, domains and functional sites

    More...
    InterProi
    View protein in InterPro
    IPR003593 AAA+_ATPase
    IPR000194 ATPase_F1/V1/A1_a/bsu_nucl-bd
    IPR011129 CSD
    IPR012340 NA-bd_OB-fold
    IPR027417 P-loop_NTPase
    IPR041703 Rho_factor_ATP-bd
    IPR011112 Rho_N
    IPR036269 Rho_N_sf
    IPR011113 Rho_RNA-bd
    IPR004665 Term_rho

    The PANTHER Classification System

    More...
    PANTHERi
    PTHR46425 PTHR46425, 1 hit

    Pfam protein domain database

    More...
    Pfami
    View protein in Pfam
    PF00006 ATP-synt_ab, 1 hit
    PF07498 Rho_N, 1 hit
    PF07497 Rho_RNA_bind, 1 hit

    Simple Modular Architecture Research Tool; a protein domain database

    More...
    SMARTi
    View protein in SMART
    SM00382 AAA, 1 hit
    SM00357 CSP, 1 hit
    SM00959 Rho_N, 1 hit

    Superfamily database of structural and functional annotation

    More...
    SUPFAMi
    SSF50249 SSF50249, 1 hit
    SSF52540 SSF52540, 1 hit
    SSF68912 SSF68912, 1 hit

    TIGRFAMs; a protein family database

    More...
    TIGRFAMsi
    TIGR00767 rho, 1 hit

    PROSITE; a protein domain and family database

    More...
    PROSITEi
    View protein in PROSITE
    PS51856 RHO_RNA_BD, 1 hit

    <p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

    <p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

    P9WHF3-1 [UniParc]FASTAAdd to basket
    « Hide
            10         20         30         40         50
    MTDTDLITAG ESTDGKPSDA AATDPPDLNA DEPAGSLATM VLPELRALAN
    60 70 80 90 100
    RAGVKGTSGM RKNELIAAIE EIRRQANGAP AVDRSAQEHD KGDRPPSSEA
    110 120 130 140 150
    PATQGEQTPT EQIDSQSQQV RPERRSATRE AGPSGSGERA GTAADDTDNR
    160 170 180 190 200
    QGGQQDAKTE ERGTDAGGDQ GGDQQASGGQ QARGDEDGEA RQGRRGRRFR
    210 220 230 240 250
    DRRRRGERSG DGAEAELRED DVVQPVAGIL DVLDNYAFVR TSGYLPGPHD
    260 270 280 290 300
    VYVSMNMVRK NGMRRGDAVT GAVRVPKEGE QPNQRQKFNP LVRLDSINGG
    310 320 330 340 350
    SVEDAKKRPE FGKLTPLYPN QRLRLETSTE RLTTRVIDLI MPIGKGQRAL
    360 370 380 390 400
    IVSPPKAGKT TILQDIANAI TRNNPECHLM VVLVDERPEE VTDMQRSVKG
    410 420 430 440 450
    EVIASTFDRP PSDHTSVAEL AIERAKRLVE QGKDVVVLLD SITRLGRAYN
    460 470 480 490 500
    NASPASGRIL SGGVDSTALY PPKRFLGAAR NIEEGGSLTI IATAMVETGS
    510 520 530 540 550
    TGDTVIFEEF KGTGNAELKL DRKIAERRVF PAVDVNPSGT RKDELLLSPD
    560 570 580 590 600
    EFAIVHKLRR VLSGLDSHQA IDLLMSQLRK TKNNYEFLVQ VSKTTPGSMD

    SD
    Length:602
    Mass (Da):65,133
    Last modified:April 16, 2014 - v1
    <p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i4E77B200810AE57F
    GO

    Sequence databases

    Select the link destinations:

    EMBL nucleotide sequence database

    More...
    EMBLi

    GenBank nucleotide sequence database

    More...
    GenBanki

    DNA Data Bank of Japan; a nucleotide sequence database

    More...
    DDBJi
    Links Updated
    AL123456 Genomic DNA Translation: CCP44054.1

    Protein sequence database of the Protein Information Resource

    More...
    PIRi
    E70773

    NCBI Reference Sequences

    More...
    RefSeqi
    NP_215813.1, NC_000962.3
    WP_003898814.1, NZ_NVQJ01000030.1

    Genome annotation databases

    Ensembl bacterial and archaeal genome annotation project

    More...
    EnsemblBacteriai
    CCP44054; CCP44054; Rv1297

    Database of genes from NCBI RefSeq genomes

    More...
    GeneIDi
    886952

    KEGG: Kyoto Encyclopedia of Genes and Genomes

    More...
    KEGGi
    mtu:Rv1297
    mtv:RVBD_1297

    <p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

    <p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AL123456 Genomic DNA Translation: CCP44054.1
    PIRiE70773
    RefSeqiNP_215813.1, NC_000962.3
    WP_003898814.1, NZ_NVQJ01000030.1

    3D structure databases

    SMRiP9WHF3
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi83332.Rv1297

    Proteomic databases

    PaxDbiP9WHF3
    PRIDEiP9WHF3

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiCCP44054; CCP44054; Rv1297
    GeneIDi886952
    KEGGimtu:Rv1297
    mtv:RVBD_1297

    Organism-specific databases

    TubercuListiRv1297

    Phylogenomic databases

    eggNOGiENOG4105C4P Bacteria
    COG1158 LUCA
    KOiK03628
    OMAiPADDHTT
    PhylomeDBiP9WHF3

    Enzyme and pathway databases

    BioCyciMTBH37RV:G185E-5471-MONOMER

    Family and domain databases

    CDDicd01128 rho_factor, 1 hit
    HAMAPiMF_01884 Rho, 1 hit
    InterProiView protein in InterPro
    IPR003593 AAA+_ATPase
    IPR000194 ATPase_F1/V1/A1_a/bsu_nucl-bd
    IPR011129 CSD
    IPR012340 NA-bd_OB-fold
    IPR027417 P-loop_NTPase
    IPR041703 Rho_factor_ATP-bd
    IPR011112 Rho_N
    IPR036269 Rho_N_sf
    IPR011113 Rho_RNA-bd
    IPR004665 Term_rho
    PANTHERiPTHR46425 PTHR46425, 1 hit
    PfamiView protein in Pfam
    PF00006 ATP-synt_ab, 1 hit
    PF07498 Rho_N, 1 hit
    PF07497 Rho_RNA_bind, 1 hit
    SMARTiView protein in SMART
    SM00382 AAA, 1 hit
    SM00357 CSP, 1 hit
    SM00959 Rho_N, 1 hit
    SUPFAMiSSF50249 SSF50249, 1 hit
    SSF52540 SSF52540, 1 hit
    SSF68912 SSF68912, 1 hit
    TIGRFAMsiTIGR00767 rho, 1 hit
    PROSITEiView protein in PROSITE
    PS51856 RHO_RNA_BD, 1 hit

    ProtoNet; Automatic hierarchical classification of proteins

    More...
    ProtoNeti
    Search...

    <p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

    <p>This subsection of the ‘Entry information’ section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiRHO_MYCTU
    <p>This subsection of the ‘Entry information’ section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called ‘Primary (citable) accession number’.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P9WHF3
    Secondary accession number(s): L0T8Z1, P66028, Q10607
    <p>This subsection of the ‘Entry information’ section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification (‘Last modified’). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 16, 2014
    Last sequence update: April 16, 2014
    Last modified: May 8, 2019
    This is version 28 of the entry and version 1 of the sequence. See complete history.
    <p>This subsection of the ‘Entry information’ section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    <p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh
      Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh: entries and gene names
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