UniProtKB - P9WH11 (RMLC_MYCTU)
Protein
dTDP-4-dehydrorhamnose 3,5-epimerase
Gene
rmlC
Organism
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Status
Functioni
Catalyzes the epimerization of the C3' and C5'positions of dTDP-6-deoxy-D-xylo-4-hexulose, forming dTDP-6-deoxy-L-lyxo-4-hexulose. Involved in the biosynthesis of the dTDP-L-rhamnose which is a component of the critical linker, D-N-acetylglucosamine-L-rhamnose disaccharide, which connects the galactan region of arabinogalactan to peptidoglycan via a phosphodiester linkage.1 Publication
Miscellaneous
Was identified as a high-confidence drug target.1 Publication
Catalytic activityi
- EC:5.1.3.131 Publication
: dTDP-L-rhamnose biosynthesis Pathwayi
This protein is involved in the pathway dTDP-L-rhamnose biosynthesis, which is part of Carbohydrate biosynthesis.By similarityView all proteins of this organism that are known to be involved in the pathway dTDP-L-rhamnose biosynthesis and in Carbohydrate biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 23 | SubstrateCombined sources1 Publication | 1 | |
Binding sitei | 28 | SubstrateCombined sources1 Publication | 1 | |
Binding sitei | 59 | SubstrateCombined sources1 Publication | 1 | |
Active sitei | 62 | Proton acceptorCombined sources1 Publication | 1 | |
Binding sitei | 72 | SubstrateCombined sources1 Publication1 Publication | 1 | |
Binding sitei | 119 | SubstrateCombined sources1 Publication1 Publication | 1 | |
Active sitei | 132 | Proton donorBy similarity | 1 | |
Sitei | 138 | Participates in a stacking interaction with the thymidine ring of dTDP-4-oxo-6-deoxyglucoseBy similarity | 1 | |
Binding sitei | 143 | SubstrateBy similarity | 1 | |
Binding sitei | 170 | SubstrateBy similarity | 1 |
GO - Molecular functioni
- dTDP-4-dehydrorhamnose 3,5-epimerase activity Source: MTBBASE
GO - Biological processi
- dTDP-rhamnose biosynthetic process Source: MTBBASE
- extracellular polysaccharide biosynthetic process Source: UniProtKB
Keywordsi
Molecular function | Isomerase |
Biological process | Carbohydrate metabolism |
Enzyme and pathway databases
BioCyci | MTBH37RV:G185E-7742-MONOMER |
UniPathwayi | UPA00124 |
Names & Taxonomyi
Protein namesi | Recommended name: dTDP-4-dehydrorhamnose 3,5-epimeraseCurated (EC:5.1.3.131 Publication)Alternative name(s): Thymidine diphospho-4-keto-rhamnose 3,5-epimeraseCurated dTDP-4-keto-6-deoxyglucose 3,5-epimerase1 Publication dTDP-6-deoxy-D-xylo-4-hexulose 3,5-epimerase1 Publication dTDP-L-rhamnose synthaseCurated |
Gene namesi | Name:rmlC Synonyms:strM Ordered Locus Names:Rv3465 |
Organismi | Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) |
Taxonomic identifieri | 83332 [NCBI] |
Taxonomic lineagei | Bacteria › Actinobacteria › Corynebacteriales › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex › |
Proteomesi |
|
Organism-specific databases
TubercuListi | Rv3465 |
Pathology & Biotechi
Chemistry databases
ChEMBLi | CHEMBL3883301 |
DrugBanki | DB04530 S,S-(2-Hydroxyethyl)Thiocysteine |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000395349 | 1 – 202 | dTDP-4-dehydrorhamnose 3,5-epimeraseAdd BLAST | 202 |
Proteomic databases
PaxDbi | P9WH11 |
PRIDEi | P9WH11 |
Interactioni
Subunit structurei
Homodimer.
3 PublicationsProtein-protein interaction databases
STRINGi | 83332.Rv3465 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P9WH11 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 47 – 49 | Substrate bindingCombined sources1 Publication | 3 |
Sequence similaritiesi
Belongs to the dTDP-4-dehydrorhamnose 3,5-epimerase family.Curated
Phylogenomic databases
eggNOGi | ENOG4108URP Bacteria COG1898 LUCA |
KOi | K01790 |
OMAi | RSILWND |
PhylomeDBi | P9WH11 |
Family and domain databases
Gene3Di | 2.60.120.10, 1 hit |
InterProi | View protein in InterPro IPR000888 dTDP_sugar_isom IPR014710 RmlC-like_jellyroll IPR011051 RmlC_Cupin_sf |
PANTHERi | PTHR21047 PTHR21047, 1 hit |
Pfami | View protein in Pfam PF00908 dTDP_sugar_isom, 1 hit |
SUPFAMi | SSF51182 SSF51182, 1 hit |
TIGRFAMsi | TIGR01221 rmlC, 1 hit |
i Sequence
Sequence statusi: Complete.
P9WH11-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKARELDVPG AWEITPTIHV DSRGLFFEWL TDHGFRAFAG HSLDVRQVNC
60 70 80 90 100
SVSSAGVLRG LHFAQLPPSQ AKYVTCVSGS VFDVVVDIRE GSPTFGRWDS
110 120 130 140 150
VLLDDQDRRT IYVSEGLAHG FLALQDNSTV MYLCSAEYNP QREHTICATD
160 170 180 190 200
PTLAVDWPLV DGAAPSLSDR DAAAPSFEDV RASGLLPRWE QTQRFIGEMR
GT
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL123456 Genomic DNA Translation: CCP46287.1 |
PIRi | F70566 |
RefSeqi | NP_217982.1, NC_000962.3 WP_003418610.1, NZ_NVQJ01000091.1 |
Genome annotation databases
EnsemblBacteriai | CCP46287; CCP46287; Rv3465 |
GeneIDi | 887352 |
KEGGi | mtu:Rv3465 mtv:RVBD_3465 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL123456 Genomic DNA Translation: CCP46287.1 |
PIRi | F70566 |
RefSeqi | NP_217982.1, NC_000962.3 WP_003418610.1, NZ_NVQJ01000091.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1PM7 | X-ray | 2.20 | A/B | 1-202 | [»] | |
1UPI | X-ray | 1.70 | A | 1-202 | [»] | |
2IXC | X-ray | 1.79 | A/B/C/D | 1-202 | [»] | |
SMRi | P9WH11 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
STRINGi | 83332.Rv3465 |
Chemistry databases
ChEMBLi | CHEMBL3883301 |
DrugBanki | DB04530 S,S-(2-Hydroxyethyl)Thiocysteine |
Proteomic databases
PaxDbi | P9WH11 |
PRIDEi | P9WH11 |
Genome annotation databases
EnsemblBacteriai | CCP46287; CCP46287; Rv3465 |
GeneIDi | 887352 |
KEGGi | mtu:Rv3465 mtv:RVBD_3465 |
Organism-specific databases
TubercuListi | Rv3465 |
Phylogenomic databases
eggNOGi | ENOG4108URP Bacteria COG1898 LUCA |
KOi | K01790 |
OMAi | RSILWND |
PhylomeDBi | P9WH11 |
Enzyme and pathway databases
UniPathwayi | UPA00124 |
BioCyci | MTBH37RV:G185E-7742-MONOMER |
Miscellaneous databases
PROi | PR:P9WH11 |
Family and domain databases
Gene3Di | 2.60.120.10, 1 hit |
InterProi | View protein in InterPro IPR000888 dTDP_sugar_isom IPR014710 RmlC-like_jellyroll IPR011051 RmlC_Cupin_sf |
PANTHERi | PTHR21047 PTHR21047, 1 hit |
Pfami | View protein in Pfam PF00908 dTDP_sugar_isom, 1 hit |
SUPFAMi | SSF51182 SSF51182, 1 hit |
TIGRFAMsi | TIGR01221 rmlC, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RMLC_MYCTU | |
Accessioni | P9WH11Primary (citable) accession number: P9WH11 Secondary accession number(s): L0TE75, O06330, Q7D5I3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 16, 2014 |
Last sequence update: | April 16, 2014 | |
Last modified: | September 18, 2019 | |
This is version 35 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Complete proteome, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families - Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh
Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh: entries and gene names - PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references