UniProtKB - P9WGZ9 (RNJ_MYCTU)
Protein
Ribonuclease J
Gene
rnj
Organism
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Status
Functioni
An RNase that has 5'-3' exonuclease and possible endonuclease activity. Involved in maturation of rRNA and in some organisms also mRNA maturation and/or decay (By similarity). Has both beta-lactamase and RNase activity, but the physiological relevance of the beta-lactamase activity, i.e. whether it confers antibiotic resistance, has not been shown (PubMed:21568871).By similarity1 Publication
Catalytic activityi
- EC:3.5.2.6
Cofactori
Zn2+UniRule annotationNote: Binds up to 2 Zn2+ ions per subunit. It is not clear if Zn2+ or Mg2+ is physiologically important.UniRule annotation
Activity regulationi
Both beta-lactamase and RNase activites are inhibited by chaperone DnaJ2.1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 81 | Zinc 1; catalyticUniRule annotation | 1 | |
Metal bindingi | 83 | Zinc 1; catalyticUniRule annotation | 1 | |
Metal bindingi | 85 | Zinc 2; catalyticUniRule annotation | 1 | |
Metal bindingi | 86 | Zinc 2; catalyticUniRule annotation | 1 | |
Metal bindingi | 148 | Zinc 1; catalyticUniRule annotation | 1 | |
Metal bindingi | 170 | Zinc 1; catalyticUniRule annotation | 1 | |
Metal bindingi | 170 | Zinc 2; catalyticUniRule annotation | 1 | |
Metal bindingi | 397 | Zinc 2; catalyticUniRule annotation | 1 |
GO - Molecular functioni
- 5'-3' exoribonuclease activity Source: UniProtKB-UniRule
- beta-lactamase activity Source: UniProtKB-EC
- endoribonuclease activity Source: UniProtKB-UniRule
- RNA binding Source: UniProtKB-UniRule
- zinc ion binding Source: InterPro
GO - Biological processi
- rRNA processing Source: UniProtKB-UniRule
Keywordsi
Molecular function | Endonuclease, Exonuclease, Hydrolase, Nuclease, RNA-binding |
Biological process | rRNA processing |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
BioCyci | MTBH37RV:G185E-7001-MONOMER |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:rnjUniRule annotation Ordered Locus Names:Rv2752c |
Organismi | Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) |
Taxonomic identifieri | 83332 [NCBI] |
Taxonomic lineagei | Bacteria › Actinobacteria › Corynebacteriales › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex › |
Proteomesi |
|
Organism-specific databases
TubercuListi | Rv2752c |
Subcellular locationi
- Cytoplasm UniRule annotation
GO - Cellular componenti
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 184 | D → A: Significantly decreased beta-lactamase and RNase activity. 1 Publication | 1 | |
Mutagenesisi | 397 | H → V: Significantly decreased beta-lactamase and RNase activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000395887 | 1 – 558 | Ribonuclease JAdd BLAST | 558 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Cross-linki | 502 | Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)1 Publication |
Keywords - PTMi
Isopeptide bond, Ubl conjugationProteomic databases
PaxDbi | P9WGZ9 |
Interactioni
Subunit structurei
Homodimer, may be a subunit of the RNA degradosome (By similarity).
Interacts with chaperone DnaJ2.
UniRule annotation1 PublicationProtein-protein interaction databases
STRINGi | 83332.Rv2752c |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 269 | Not absolutely required for RNase activityAdd BLAST | 269 | |
Regioni | 371 – 375 | Substrate bindingUniRule annotation | 5 | |
Regioni | 451 – 558 | Required for beta-lactamase activityAdd BLAST | 108 |
Sequence similaritiesi
Belongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family. Bacterial RNase J subfamily.UniRule annotation
Phylogenomic databases
eggNOGi | ENOG4105CN5 Bacteria COG0595 LUCA |
KOi | K12574 |
OMAi | KNTCVFE |
PhylomeDBi | P9WGZ9 |
Family and domain databases
Gene3Di | 3.40.50.10710, 1 hit 3.60.15.10, 1 hit |
HAMAPi | MF_01491 RNase_J_bact, 1 hit |
InterProi | View protein in InterPro IPR001279 Metallo-B-lactamas IPR036866 RibonucZ/Hydroxyglut_hydro IPR011108 RMMBL IPR004613 RNase_J IPR042173 RNase_J_2 IPR030854 RNase_J_bac IPR041636 RNase_J_C |
Pfami | View protein in Pfam PF12706 Lactamase_B_2, 1 hit PF07521 RMMBL, 1 hit PF17770 RNase_J_C, 1 hit |
PIRSFi | PIRSF004803 RnjA, 1 hit |
SMARTi | View protein in SMART SM00849 Lactamase_B, 1 hit |
SUPFAMi | SSF56281 SSF56281, 1 hit |
TIGRFAMsi | TIGR00649 MG423, 1 hit |
i Sequence
Sequence statusi: Complete.
P9WGZ9-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MDVDLPPPGP LTSGGLRVTA LGGINEIGRN MTVFEHLGRL LIIDCGVLFP
60 70 80 90 100
GHDEPGVDLI LPDMRHVEDR LDDIEALVLT HGHEDHIGAI PFLLKLRPDI
110 120 130 140 150
PVVGSKFTLA LVAEKCREYR ITPVFVEVRE GQSTRHGVFE CEYFAVNHST
160 170 180 190 200
PDALAIAVYT GAGTILHTGD IKFDQLPPDG RPTDLPGMSR LGDTGVDLLL
210 220 230 240 250
CDSTNAEIPG VGPSESEVGP TLHRLIRGAD GRVIVACFAS NVDRVQQIID
260 270 280 290 300
AAVALGRRVS FVGRSMVRNM RVARQLGFLR VADSDLIDIA AAETMAPDQV
310 320 330 340 350
VLITTGTQGE PMSALSRMSR GEHRSITLTA GDLIVLSSSL IPGNEEAVFG
360 370 380 390 400
VIDALSKIGA RVVTNAQARV HVSGHAYAGE LLFLYNGVRP RNVMPVHGTW
410 420 430 440 450
RMLRANAKLA ASTGVPQESI LLAENGVSVD LVAGKASISG AVPVGKMFVD
460 470 480 490 500
GLIAGDVGDI TLGERLILSS GFVAVTVVVR RGTGQPLAAP HLHSRGFSED
510 520 530 540 550
PKALEPAVRK VEAELESLVA ANVTDPIRIA QGVRRTVGKW VGETYRRQPM
IVPTVIEV
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL123456 Genomic DNA Translation: CCP45551.1 |
PIRi | G70879 |
RefSeqi | NP_217268.1, NC_000962.3 WP_003414049.1, NZ_NVQJ01000020.1 |
Genome annotation databases
EnsemblBacteriai | CCP45551; CCP45551; Rv2752c |
GeneIDi | 887802 |
KEGGi | mtu:Rv2752c mtv:RVBD_2752c |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL123456 Genomic DNA Translation: CCP45551.1 |
PIRi | G70879 |
RefSeqi | NP_217268.1, NC_000962.3 WP_003414049.1, NZ_NVQJ01000020.1 |
3D structure databases
SMRi | P9WGZ9 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 83332.Rv2752c |
Proteomic databases
PaxDbi | P9WGZ9 |
Genome annotation databases
EnsemblBacteriai | CCP45551; CCP45551; Rv2752c |
GeneIDi | 887802 |
KEGGi | mtu:Rv2752c mtv:RVBD_2752c |
Organism-specific databases
TubercuListi | Rv2752c |
Phylogenomic databases
eggNOGi | ENOG4105CN5 Bacteria COG0595 LUCA |
KOi | K12574 |
OMAi | KNTCVFE |
PhylomeDBi | P9WGZ9 |
Enzyme and pathway databases
BioCyci | MTBH37RV:G185E-7001-MONOMER |
Family and domain databases
Gene3Di | 3.40.50.10710, 1 hit 3.60.15.10, 1 hit |
HAMAPi | MF_01491 RNase_J_bact, 1 hit |
InterProi | View protein in InterPro IPR001279 Metallo-B-lactamas IPR036866 RibonucZ/Hydroxyglut_hydro IPR011108 RMMBL IPR004613 RNase_J IPR042173 RNase_J_2 IPR030854 RNase_J_bac IPR041636 RNase_J_C |
Pfami | View protein in Pfam PF12706 Lactamase_B_2, 1 hit PF07521 RMMBL, 1 hit PF17770 RNase_J_C, 1 hit |
PIRSFi | PIRSF004803 RnjA, 1 hit |
SMARTi | View protein in SMART SM00849 Lactamase_B, 1 hit |
SUPFAMi | SSF56281 SSF56281, 1 hit |
TIGRFAMsi | TIGR00649 MG423, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RNJ_MYCTU | |
Accessioni | P9WGZ9Primary (citable) accession number: P9WGZ9 Secondary accession number(s): L0TAS3, O33294, Q7D6N2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 16, 2014 |
Last sequence update: | April 16, 2014 | |
Last modified: | September 18, 2019 | |
This is version 34 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Complete proteome, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families - Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh
Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh: entries and gene names