UniProtKB - P9WGM5 (NARL_MYCTU)
Protein
Probable transcriptional regulatory protein NarL
Gene
narL
Organism
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Status
Functioni
Member of the two-component regulatory system NarS/NarL that regulates genes involved in aerobic nitrate metabolism (PubMed:25659431). Upon phosphorylation by NarS, functions as a transcription regulator by direct binding to promoter regions of target genes together with DevR to regulate their expression during aerobic nitrate metabolism (PubMed:25659431).1 Publication
Miscellaneous
Was identified as a high-confidence drug target.
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
DNA bindingi | 172 – 191 | H-T-H motifPROSITE-ProRule annotationAdd BLAST | 20 |
GO - Molecular functioni
- DNA binding Source: UniProtKB-KW
GO - Biological processi
- phosphorelay signal transduction system Source: UniProtKB-KW
- regulation of transcription, DNA-templated Source: InterPro
Keywordsi
Molecular function | DNA-binding |
Biological process | Transcription, Transcription regulation, Two-component regulatory system |
Names & Taxonomyi
Protein namesi | Recommended name: Probable transcriptional regulatory protein NarL |
Gene namesi | Name:narL Ordered Locus Names:Rv0844c |
Organismi | Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) |
Taxonomic identifieri | 83332 [NCBI] |
Taxonomic lineagei | Bacteria › Actinobacteria › Corynebacteriales › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex › |
Proteomesi |
|
Organism-specific databases
TubercuListi | Rv0844c |
Subcellular locationi
- Cytoplasm Curated
GO - Cellular componenti
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Disruption phenotypei
Mutants show no change in virulence in mouse model of infection.1 Publication
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 61 | D → N: Complete loss of phosphorylation. 1 Publication | 1 |
Miscellaneous databases
PHI-basei | PHI:3620 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000401134 | 1 – 216 | Probable transcriptional regulatory protein NarLAdd BLAST | 216 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 61 | 4-aspartylphosphatePROSITE-ProRule annotation1 Publication | 1 |
Post-translational modificationi
Phosphorylated by NarS.1 Publication
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | P9WGM5 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P9WGM5 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 10 – 126 | Response regulatoryPROSITE-ProRule annotationAdd BLAST | 117 | |
Domaini | 148 – 213 | HTH luxR-typePROSITE-ProRule annotationAdd BLAST | 66 |
Phylogenomic databases
eggNOGi | COG2197, Bacteria |
OMAi | IAFDMGI |
PhylomeDBi | P9WGM5 |
Family and domain databases
CDDi | cd06170, LuxR_C_like, 1 hit cd00156, REC, 1 hit |
InterProi | View protein in InterPro IPR011006, CheY-like_superfamily IPR016032, Sig_transdc_resp-reg_C-effctor IPR001789, Sig_transdc_resp-reg_receiver IPR000792, Tscrpt_reg_LuxR_C |
Pfami | View protein in Pfam PF00196, GerE, 1 hit PF00072, Response_reg, 1 hit |
PRINTSi | PR00038, HTHLUXR |
SMARTi | View protein in SMART SM00421, HTH_LUXR, 1 hit SM00448, REC, 1 hit |
SUPFAMi | SSF46894, SSF46894, 1 hit SSF52172, SSF52172, 1 hit |
PROSITEi | View protein in PROSITE PS50043, HTH_LUXR_2, 1 hit PS50110, RESPONSE_REGULATORY, 1 hit |
i Sequence
Sequence statusi: Complete.
P9WGM5-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSNPQPEKVR VVVGDDHPLF REGVVRALSL SGSVNVVGEA DDGAAALELI
60 70 80 90 100
KAHLPDVALL DYRMPGMDGA QVAAAVRSYE LPTRVLLISA HDEPAIVYQA
110 120 130 140 150
LQQGAAGFLL KDSTRTEIVK AVLDCAKGRD VVAPSLVGGL AGEIRQRAAP
160 170 180 190 200
VAPVLSARER EVLNRIACGQ SIPAIAAELY VAPSTVKTHV QRLYEKLGVS
210
DRAAAVAEAM RQRLLD
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL123456 Genomic DNA Translation: CCP43592.1 |
PIRi | D70813 |
RefSeqi | NP_215359.1, NC_000962.3 WP_003404388.1, NZ_NVQJ01000040.1 |
Genome annotation databases
EnsemblBacteriai | CCP43592; CCP43592; Rv0844c |
GeneIDi | 45424810 885603 |
KEGGi | mtu:Rv0844c |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL123456 Genomic DNA Translation: CCP43592.1 |
PIRi | D70813 |
RefSeqi | NP_215359.1, NC_000962.3 WP_003404388.1, NZ_NVQJ01000040.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3EUL | X-ray | 1.90 | A/B/C/D | 1-145 | [»] | |
SMRi | P9WGM5 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
STRINGi | 83332.Rv0844c |
Proteomic databases
PaxDbi | P9WGM5 |
Genome annotation databases
EnsemblBacteriai | CCP43592; CCP43592; Rv0844c |
GeneIDi | 45424810 885603 |
KEGGi | mtu:Rv0844c |
Organism-specific databases
TubercuListi | Rv0844c |
Phylogenomic databases
eggNOGi | COG2197, Bacteria |
OMAi | IAFDMGI |
PhylomeDBi | P9WGM5 |
Miscellaneous databases
PHI-basei | PHI:3620 |
Family and domain databases
CDDi | cd06170, LuxR_C_like, 1 hit cd00156, REC, 1 hit |
InterProi | View protein in InterPro IPR011006, CheY-like_superfamily IPR016032, Sig_transdc_resp-reg_C-effctor IPR001789, Sig_transdc_resp-reg_receiver IPR000792, Tscrpt_reg_LuxR_C |
Pfami | View protein in Pfam PF00196, GerE, 1 hit PF00072, Response_reg, 1 hit |
PRINTSi | PR00038, HTHLUXR |
SMARTi | View protein in SMART SM00421, HTH_LUXR, 1 hit SM00448, REC, 1 hit |
SUPFAMi | SSF46894, SSF46894, 1 hit SSF52172, SSF52172, 1 hit |
PROSITEi | View protein in PROSITE PS50043, HTH_LUXR_2, 1 hit PS50110, RESPONSE_REGULATORY, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | NARL_MYCTU | |
Accessioni | P9WGM5Primary (citable) accession number: P9WGM5 Secondary accession number(s): L0T7W4, O53856, Q7D965 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 16, 2014 |
Last sequence update: | April 16, 2014 | |
Last modified: | February 10, 2021 | |
This is version 36 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references