UniProtKB - P9WGL1 (MPRB_MYCTU)
Protein
Signal transduction histidine-protein kinase/phosphatase MprB
Gene
mprB
Organism
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Status
Functioni
Member of the two-component regulatory system MprB/MprA which contributes to maintaining a balance among several systems involved in stress resistance and is required for establishment and maintenance of persistent infection in the host. In response to environmental signals MprB acts as both a membrane-associated protein kinase that undergoes autophosphorylation and subsequently transfers the phosphate to MprA, and a protein phosphatase that dephosphorylates phospho-MprA. MprB/MprA is involved in regulation of numerous stress-responsive genes, including up-regulation of two sigma factors, sigE and sigB as well as pepD and mprA, and repression of multiple genes from regulons associated with hypoxia, starvation and iron metabolism. The majority of genes regulated by MprB/MprA under a particular stress condition are different from those induced during normal growth, but several genes are commonly regulated under more than one condition.5 Publications
Miscellaneous
Deletion experiments show that amino acids 95-133 are required for activation of MprA.
Was identified as a high-confidence drug target.
Catalytic activityi
- ATP + protein L-histidine = ADP + protein N-phospho-L-histidine. EC:2.7.13.3
Cofactori
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- kinase activity Source: MTBBASE
- phosphoprotein phosphatase activity Source: MTBBASE
- phosphorelay sensor kinase activity Source: GO_Central
GO - Biological processi
- pathogenesis Source: UniProtKB-KW
- peptidyl-histidine phosphorylation Source: MTBBASE
- phosphorelay signal transduction system Source: MTBBASE
- regulation of response to osmotic stress Source: GO_Central
Keywordsi
Molecular function | Hydrolase, Kinase, Protein phosphatase, Transferase |
Biological process | Stress response, Two-component regulatory system, Virulence |
Ligand | ATP-binding, Magnesium, Manganese, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | MTBH37RV:G185E-5139-MONOMER |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:mprB Ordered Locus Names:Rv0982 |
Organismi | Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) |
Taxonomic identifieri | 83332 [NCBI] |
Taxonomic lineagei | Bacteria › Actinobacteria › Corynebacteriales › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex › |
Proteomesi |
|
Organism-specific databases
TubercuListi | Rv0982 |
Subcellular locationi
- Cell membrane Curated; Multi-pass membrane protein Curated
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 26 | CytoplasmicSequence analysisAdd BLAST | 26 | |
Transmembranei | 27 – 47 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 48 – 163 | ExtracellularSequence analysisAdd BLAST | 116 | |
Transmembranei | 164 – 184 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 185 – 504 | CytoplasmicSequence analysisAdd BLAST | 320 |
GO - Cellular componenti
- extracellular region Source: MTBBASE
- integral component of plasma membrane Source: GO_Central
- plasma membrane Source: MTBBASE
Keywords - Cellular componenti
Cell membrane, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 249 | H → Q: Abolishes autophosphorylation as well as dephosphorylation of phospho-MprA. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000308440 | 1 – 504 | Signal transduction histidine-protein kinase/phosphatase MprBAdd BLAST | 504 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 249 | Phosphohistidine; by autocatalysisCurated | 1 |
Post-translational modificationi
Autophosphorylated.
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | P9WGL1 |
PRIDEi | P9WGL1 |
Expressioni
Inductioni
Induced by MprA. Differentially up-regulated under different stress conditions, such as low concentrations of detergents and alkaline pH. Induced by low concentrations of sodium dodecyl sulfate (SDS) in a SigE-dependent manner. In strain ATCC 25618 / H37Rv, repressed during growth in macrophages. Highly up-regulated during the early stages of invasion of the human blood-brain barrier.5 Publications
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 186 – 238 | HAMPPROSITE-ProRule annotationAdd BLAST | 53 | |
Domaini | 246 – 466 | Histidine kinasePROSITE-ProRule annotationAdd BLAST | 221 |
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | ENOG41062D9 Bacteria COG0642 LUCA |
KOi | K07653 |
OMAi | HELYEPL |
PhylomeDBi | P9WGL1 |
Family and domain databases
CDDi | cd06225 HAMP, 1 hit cd00082 HisKA, 1 hit |
Gene3Di | 3.30.565.10, 1 hit |
InterProi | View protein in InterPro IPR003660 HAMP_dom IPR003594 HATPase_C IPR036890 HATPase_C_sf IPR005467 His_kinase_dom IPR003661 HisK_dim/P IPR036097 HisK_dim/P_sf IPR004358 Sig_transdc_His_kin-like_C |
Pfami | View protein in Pfam PF00672 HAMP, 1 hit PF02518 HATPase_c, 1 hit PF00512 HisKA, 1 hit |
PRINTSi | PR00344 BCTRLSENSOR |
SMARTi | View protein in SMART SM00304 HAMP, 1 hit SM00387 HATPase_c, 1 hit SM00388 HisKA, 1 hit |
SUPFAMi | SSF47384 SSF47384, 1 hit SSF55874 SSF55874, 1 hit |
PROSITEi | View protein in PROSITE PS50885 HAMP, 1 hit PS50109 HIS_KIN, 1 hit |
i Sequence
Sequence statusi: Complete.
P9WGL1-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MWWFRRRDRA PLRATSSLSL RWRVMLLAMS MVAMVVVLMS FAVYAVISAA
60 70 80 90 100
LYSDIDNQLQ SRAQLLIASG SLAADPGKAI EGTAYSDVNA MLVNPGQSIY
110 120 130 140 150
TAQQPGQTLP VGAAEKAVIR GELFMSRRTT ADQRVLAIRL TNGSSLLISK
160 170 180 190 200
SLKPTEAVMN KLRWVLLIVG GIGVAVAAVA GGMVTRAGLR PVGRLTEAAE
210 220 230 240 250
RVARTDDLRP IPVFGSDELA RLTEAFNLML RALAESRERQ ARLVTDAGHE
260 270 280 290 300
LRTPLTSLRT NVELLMASMA PGAPRLPKQE MVDLRADVLA QIEELSTLVG
310 320 330 340 350
DLVDLSRGDA GEVVHEPVDM ADVVDRSLER VRRRRNDILF DVEVIGWQVY
360 370 380 390 400
GDTAGLSRMA LNLMDNAAKW SPPGGHVGVR LSQLDASHAE LVVSDRGPGI
410 420 430 440 450
PVQERRLVFE RFYRSASARA LPGSGLGLAI VKQVVLNHGG LLRIEDTDPG
460 470 480 490 500
GQPPGTSIYV LLPGRRMPIP QLPGATAGAR STDIENSRGS ANVISVESQS
TRAT
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL123456 Genomic DNA Translation: CCP43732.1 |
PIRi | B70821 |
RefSeqi | NP_215497.1, NC_000962.3 WP_003911317.1, NZ_NVQJ01000018.1 |
Genome annotation databases
EnsemblBacteriai | CCP43732; CCP43732; Rv0982 |
GeneIDi | 885062 |
KEGGi | mtu:Rv0982 mtv:RVBD_0982 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL123456 Genomic DNA Translation: CCP43732.1 |
PIRi | B70821 |
RefSeqi | NP_215497.1, NC_000962.3 WP_003911317.1, NZ_NVQJ01000018.1 |
3D structure databases
SMRi | P9WGL1 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 83332.Rv0982 |
Proteomic databases
PaxDbi | P9WGL1 |
PRIDEi | P9WGL1 |
Genome annotation databases
EnsemblBacteriai | CCP43732; CCP43732; Rv0982 |
GeneIDi | 885062 |
KEGGi | mtu:Rv0982 mtv:RVBD_0982 |
Organism-specific databases
TubercuListi | Rv0982 |
Phylogenomic databases
eggNOGi | ENOG41062D9 Bacteria COG0642 LUCA |
KOi | K07653 |
OMAi | HELYEPL |
PhylomeDBi | P9WGL1 |
Enzyme and pathway databases
BioCyci | MTBH37RV:G185E-5139-MONOMER |
Family and domain databases
CDDi | cd06225 HAMP, 1 hit cd00082 HisKA, 1 hit |
Gene3Di | 3.30.565.10, 1 hit |
InterProi | View protein in InterPro IPR003660 HAMP_dom IPR003594 HATPase_C IPR036890 HATPase_C_sf IPR005467 His_kinase_dom IPR003661 HisK_dim/P IPR036097 HisK_dim/P_sf IPR004358 Sig_transdc_His_kin-like_C |
Pfami | View protein in Pfam PF00672 HAMP, 1 hit PF02518 HATPase_c, 1 hit PF00512 HisKA, 1 hit |
PRINTSi | PR00344 BCTRLSENSOR |
SMARTi | View protein in SMART SM00304 HAMP, 1 hit SM00387 HATPase_c, 1 hit SM00388 HisKA, 1 hit |
SUPFAMi | SSF47384 SSF47384, 1 hit SSF55874 SSF55874, 1 hit |
PROSITEi | View protein in PROSITE PS50885 HAMP, 1 hit PS50109 HIS_KIN, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MPRB_MYCTU | |
Accessioni | P9WGL1Primary (citable) accession number: P9WGL1 Secondary accession number(s): L0T8A3, O53895, Q7D913 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 16, 2014 |
Last sequence update: | April 16, 2014 | |
Last modified: | September 18, 2019 | |
This is version 34 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Complete proteome, Reference proteomeDocuments
- Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh
Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh: entries and gene names