UniProtKB - P9WGK6 (PRRB_MYCTO)
Protein
Sensor-type histidine kinase PrrB
Gene
prrB
Organism
Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh)
Status
Functioni
Member of the two-component regulatory system PrrB/PrrA that is involved specifically in early intracellular multiplication of Mycobacterium and is essential for its viability. Functions as a sensor protein kinase which is autophosphorylated at a histidine residue and transfers its phosphate group to the conserved aspartic acid residue in the regulatory domain of PrrA. In turn, PrrA binds to the upstream promoter regions of target genes including itself to positively regulate their expression.By similarity
Catalytic activityi
- ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.By similarity EC:2.7.13.3
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- phosphorelay sensor kinase activity Source: InterPro
Keywordsi
Molecular function | Kinase, Transferase |
Biological process | Two-component regulatory system |
Ligand | ATP-binding, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:prrB Ordered Locus Names:MT0925 |
Organismi | Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh) |
Taxonomic identifieri | 83331 [NCBI] |
Taxonomic lineagei | Bacteria › Actinobacteria › Corynebacteriales › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex › |
Proteomesi |
|
Subcellular locationi
- Cell membrane Curated; Multi-pass membrane protein Curated
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 19 – 39 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 151 – 171 | HelicalSequence analysisAdd BLAST | 21 |
GO - Cellular componenti
- integral component of membrane Source: UniProtKB-KW
- plasma membrane Source: UniProtKB-SubCell
Keywords - Cellular componenti
Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000428345 | 1 – 446 | Sensor-type histidine kinase PrrBAdd BLAST | 446 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 240 | Phosphohistidine; by autocatalysisPROSITE-ProRule annotation | 1 |
Post-translational modificationi
Autophosphorylated.By similarity
Keywords - PTMi
PhosphoproteinFamily & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 172 – 222 | HAMPPROSITE-ProRule annotationAdd BLAST | 51 | |
Domaini | 237 – 446 | Histidine kinasePROSITE-ProRule annotationAdd BLAST | 210 |
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
HOGENOMi | CLU_026160_0_0_11 |
Family and domain databases
CDDi | cd06225, HAMP, 1 hit cd00082, HisKA, 1 hit |
Gene3Di | 3.30.565.10, 1 hit |
InterProi | View protein in InterPro IPR003660, HAMP_dom IPR003594, HATPase_C IPR036890, HATPase_C_sf IPR005467, His_kinase_dom IPR003661, HisK_dim/P IPR036097, HisK_dim/P_sf IPR004358, Sig_transdc_His_kin-like_C |
Pfami | View protein in Pfam PF00672, HAMP, 1 hit PF02518, HATPase_c, 1 hit PF00512, HisKA, 1 hit |
PRINTSi | PR00344, BCTRLSENSOR |
SMARTi | View protein in SMART SM00304, HAMP, 1 hit SM00387, HATPase_c, 1 hit SM00388, HisKA, 1 hit |
SUPFAMi | SSF47384, SSF47384, 1 hit SSF55874, SSF55874, 1 hit |
PROSITEi | View protein in PROSITE PS50885, HAMP, 1 hit PS50109, HIS_KIN, 1 hit |
i Sequence
Sequence statusi: Complete.
P9WGK6-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MNILSRIFAR TPSLRTRVVV ATAIGAAIPV LIVGTVVWVG ITNDRKERLD
60 70 80 90 100
RRLDEAAGFA IPFVPRGLDE IPRSPNDQDA LITVRRGNVI KSNSDITLPK
110 120 130 140 150
LQDDYADTYV RGVRYRVRTV EIPGPEPTSV AVGATYDATV AETNNLHRRV
160 170 180 190 200
LLICTFAIGA AAVFAWLLAA FAVRPFKQLA EQTRSIDAGD EAPRVEVHGA
210 220 230 240 250
SEAIEIAEAM RGMLQRIWNE QNRTKEALAS ARDFAAVSSH ELRTPLTAMR
260 270 280 290 300
TNLEVLSTLD LPDDQRKEVL NDVIRTQSRI EATLSALERL AQGELSTSDD
310 320 330 340 350
HVPVDITDLL DRAAHDAARI YPDLDVSLVP SPTCIIVGLP AGLRLAVDNA
360 370 380 390 400
IANAVKHGGA TLVQLSAVSS RAGVEIAIDD NGSGVPEGER QVVFERFSRG
410 420 430 440
STASHSGSGL GLALVAQQAQ LHGGTASLEN SPLGGARLVL RLPGPS
Sequence cautioni
The sequence AAK45172 differs from that shown. Reason: Erroneous initiation.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE000516 Genomic DNA Translation: AAK45172.1 Different initiation. |
PIRi | C70783 |
RefSeqi | WP_003404689.1, NZ_KK341227.1 |
Genome annotation databases
EnsemblBacteriai | AAK45172; AAK45172; MT0925 |
GeneIDi | 23492974 |
KEGGi | mtc:MT0925 |
PATRICi | fig|83331.31.peg.994 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE000516 Genomic DNA Translation: AAK45172.1 Different initiation. |
PIRi | C70783 |
RefSeqi | WP_003404689.1, NZ_KK341227.1 |
3D structure databases
SMRi | P9WGK6 |
ModBasei | Search... |
Genome annotation databases
EnsemblBacteriai | AAK45172; AAK45172; MT0925 |
GeneIDi | 23492974 |
KEGGi | mtc:MT0925 |
PATRICi | fig|83331.31.peg.994 |
Phylogenomic databases
HOGENOMi | CLU_026160_0_0_11 |
Family and domain databases
CDDi | cd06225, HAMP, 1 hit cd00082, HisKA, 1 hit |
Gene3Di | 3.30.565.10, 1 hit |
InterProi | View protein in InterPro IPR003660, HAMP_dom IPR003594, HATPase_C IPR036890, HATPase_C_sf IPR005467, His_kinase_dom IPR003661, HisK_dim/P IPR036097, HisK_dim/P_sf IPR004358, Sig_transdc_His_kin-like_C |
Pfami | View protein in Pfam PF00672, HAMP, 1 hit PF02518, HATPase_c, 1 hit PF00512, HisKA, 1 hit |
PRINTSi | PR00344, BCTRLSENSOR |
SMARTi | View protein in SMART SM00304, HAMP, 1 hit SM00387, HATPase_c, 1 hit SM00388, HisKA, 1 hit |
SUPFAMi | SSF47384, SSF47384, 1 hit SSF55874, SSF55874, 1 hit |
PROSITEi | View protein in PROSITE PS50885, HAMP, 1 hit PS50109, HIS_KIN, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PRRB_MYCTO | |
Accessioni | P9WGK6Primary (citable) accession number: P9WGK6 Secondary accession number(s): L0T543, P0A5Z8, Q10560 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 16, 2014 |
Last sequence update: | April 16, 2014 | |
Last modified: | December 2, 2020 | |
This is version 32 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |