UniProtKB - P9WGE5 (SPEE_MYCTU)
Protein
Polyamine aminopropyltransferase
Gene
speE
Organism
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Status
Functioni
Catalyzes the irreversible transfer of a propylamine group from the amino donor S-adenosylmethioninamine (decarboxy-AdoMet) to putrescine (1,4-diaminobutane) to yield spermidine.UniRule annotation
Miscellaneous
Was identified as a high-confidence drug target.
Catalytic activityi
- putrescine + S-adenosyl 3-(methylsulfanyl)propylamine = H+ + S-methyl-5'-thioadenosine + spermidineUniRule annotationEC:2.5.1.16UniRule annotation
: spermidine biosynthesis Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes spermidine from putrescine.UniRule annotationProteins known to be involved in this subpathway in this organism are:
- Polyamine aminopropyltransferase (speE)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes spermidine from putrescine, the pathway spermidine biosynthesis and in Amine and polyamine biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 261 | S-adenosylmethioninamineUniRule annotation | 1 | |
Binding sitei | 313 | PolyamineUniRule annotation | 1 | |
Binding sitei | 333 | S-adenosylmethioninamineUniRule annotation | 1 | |
Active sitei | 386 | Proton acceptorUniRule annotation | 1 |
GO - Molecular functioni
- spermidine synthase activity Source: UniProtKB-UniRule
GO - Biological processi
- polyamine biosynthetic process Source: GO_Central
- spermidine biosynthetic process Source: UniProtKB-UniRule
Keywordsi
Molecular function | Transferase |
Biological process | Polyamine biosynthesis, Spermidine biosynthesis |
Enzyme and pathway databases
BioCyci | MTBH37RV:G185E-6843-MONOMER |
UniPathwayi | UPA00248;UER00314 |
Names & Taxonomyi
Protein namesi | Recommended name: Polyamine aminopropyltransferaseUniRule annotationAlternative name(s): Putrescine aminopropyltransferaseUniRule annotation Short name: PAPTUniRule annotation Spermidine synthaseUniRule annotation (EC:2.5.1.16UniRule annotation) Short name: SPDSUniRule annotation Short name: SPDSYUniRule annotation |
Gene namesi | Name:speEUniRule annotation Ordered Locus Names:Rv2601 ORF Names:MTCI270.04c, MTV001.02 |
Organismi | Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) |
Taxonomic identifieri | 83332 [NCBI] |
Taxonomic lineagei | Bacteria › Actinobacteria › Corynebacteriales › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex › |
Proteomesi |
|
Organism-specific databases
TubercuListi | Rv2601 |
Subcellular locationi
- Cell membrane UniRule annotation; Multi-pass membrane protein UniRule annotation
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 20 – 40 | HelicalUniRule annotationAdd BLAST | 21 | |
Transmembranei | 51 – 71 | HelicalUniRule annotationAdd BLAST | 21 | |
Transmembranei | 88 – 108 | HelicalUniRule annotationAdd BLAST | 21 | |
Transmembranei | 116 – 136 | HelicalUniRule annotationAdd BLAST | 21 | |
Transmembranei | 164 – 184 | HelicalUniRule annotationAdd BLAST | 21 | |
Transmembranei | 186 – 206 | HelicalUniRule annotationAdd BLAST | 21 | |
Transmembranei | 215 – 235 | HelicalUniRule annotationAdd BLAST | 21 |
GO - Cellular componenti
- cell wall Source: MTBBASE
- integral component of membrane Source: UniProtKB-KW
- plasma membrane Source: UniProtKB-SubCell
Keywords - Cellular componenti
Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000156488 | 1 – 523 | Polyamine aminopropyltransferaseAdd BLAST | 523 |
Proteomic databases
PaxDbi | P9WGE5 |
PRIDEi | P9WGE5 |
Interactioni
Subunit structurei
Homodimer or homotetramer.
UniRule annotationProtein-protein interaction databases
STRINGi | 83332.Rv2601 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 231 – 465 | PABSUniRule annotationAdd BLAST | 235 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 203 – 478 | Spermidine synthaseAdd BLAST | 276 | |
Regioni | 280 – 281 | Polyamine bindingUniRule annotation | 2 | |
Regioni | 365 – 366 | S-adenosylmethioninamine bindingUniRule annotation | 2 |
Sequence similaritiesi
Belongs to the spermidine/spermine synthase family.UniRule annotation
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | ENOG4108JPV Bacteria COG4262 LUCA |
KOi | K00797 |
OMAi | SFGEWGY |
PhylomeDBi | P9WGE5 |
Family and domain databases
HAMAPi | MF_00198 Spermidine_synth, 1 hit |
InterProi | View protein in InterPro IPR030374 PABS IPR030373 PABS_CS IPR029063 SAM-dependent_MTases IPR001045 Spermi_synthase |
SUPFAMi | SSF53335 SSF53335, 1 hit |
PROSITEi | View protein in PROSITE PS01330 PABS_1, 1 hit PS51006 PABS_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P9WGE5-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTSTRQAGEA TEASVRWRAV LLAAVAACAA CGLVYELALL TLAASLNGGG
60 70 80 90 100
IVATSLIVAG YIAALGAGAL LIKPLLAHAA IAFIAVEAVL GIIGGLSAAA
110 120 130 140 150
LYAAFAFLDE LDGSTLVLAV GTALIGGLVG AEVPLLMTLL QRGRVAGAAD
160 170 180 190 200
AGRTLANLNA ADYLGALVGG LAWPFLLLPQ LGMIRGAAVT GIVNLAAAGV
210 220 230 240 250
VSIFLLRHVV SGRQLVTALC ALAAALGLIA TLLVHSHDIE TTGRQQLYAD
260 270 280 290 300
PIIAYRHSAY QEIVVTRRGD DLRLYLDGGL QFCTRDEYRY TESLVYPAVS
310 320 330 340 350
DGARSVLVLG GGDGLAAREL LRQPGIEQIV QVELDPAVIE LARTTLRDVN
360 370 380 390 400
AGSLDNPRVH VVIDDAMSWL RGAAVPPAGF DAVIVDLRDP DTPVLGRLYS
410 420 430 440 450
TEFYALAARA LAPGGLMVVQ AGSPYSTPTA FWRIISTIRS AGYAVTPYHV
460 470 480 490 500
HVPTFGDWGF ALARLTDIAP TPAVPSTAPA LRFLDQQVLE AATVFSGDIR
510 520
PRTLDPSTLD NPHIVEDMRH GWD
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL123456 Genomic DNA Translation: CCP45397.1 |
PIRi | H70886 |
RefSeqi | WP_003899392.1, NZ_NVQJ01000023.1 YP_177892.1, NC_000962.3 |
Genome annotation databases
EnsemblBacteriai | CCP45397; CCP45397; Rv2601 |
GeneIDi | 887676 |
KEGGi | mtu:Rv2601 mtv:RVBD_2601 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL123456 Genomic DNA Translation: CCP45397.1 |
PIRi | H70886 |
RefSeqi | WP_003899392.1, NZ_NVQJ01000023.1 YP_177892.1, NC_000962.3 |
3D structure databases
SMRi | P9WGE5 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 83332.Rv2601 |
Proteomic databases
PaxDbi | P9WGE5 |
PRIDEi | P9WGE5 |
Genome annotation databases
EnsemblBacteriai | CCP45397; CCP45397; Rv2601 |
GeneIDi | 887676 |
KEGGi | mtu:Rv2601 mtv:RVBD_2601 |
Organism-specific databases
TubercuListi | Rv2601 |
Phylogenomic databases
eggNOGi | ENOG4108JPV Bacteria COG4262 LUCA |
KOi | K00797 |
OMAi | SFGEWGY |
PhylomeDBi | P9WGE5 |
Enzyme and pathway databases
UniPathwayi | UPA00248;UER00314 |
BioCyci | MTBH37RV:G185E-6843-MONOMER |
Family and domain databases
HAMAPi | MF_00198 Spermidine_synth, 1 hit |
InterProi | View protein in InterPro IPR030374 PABS IPR030373 PABS_CS IPR029063 SAM-dependent_MTases IPR001045 Spermi_synthase |
SUPFAMi | SSF53335 SSF53335, 1 hit |
PROSITEi | View protein in PROSITE PS01330 PABS_1, 1 hit PS51006 PABS_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SPEE_MYCTU | |
Accessioni | P9WGE5Primary (citable) accession number: P9WGE5 Secondary accession number(s): L0TD16, O33279 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 16, 2014 |
Last sequence update: | April 16, 2014 | |
Last modified: | May 8, 2019 | |
This is version 28 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Complete proteome, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families - Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh
Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh: entries and gene names - PATHWAY comments
Index of metabolic and biosynthesis pathways