UniProtKB - P9WFX5 (TRPD_MYCTU)
Protein
Anthranilate phosphoribosyltransferase
Gene
trpD
Organism
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Status
Functioni
Catalyzes the transfer of the phosphoribosyl group of 5-phosphorylribose-1-pyrophosphate (PRPP) to anthranilate to yield N-(5'-phosphoribosyl)-anthranilate (PRA).
Catalytic activityi
- diphosphate + N-(5-phospho-β-D-ribosyl)anthranilate = 5-phospho-α-D-ribose 1-diphosphate + anthranilateUniRule annotationEC:2.4.2.18UniRule annotation
Cofactori
Mg2+UniRule annotation4 PublicationsNote: Binds 2 magnesium ions per monomer.UniRule annotation4 Publications
: L-tryptophan biosynthesis Pathwayi
This protein is involved in step 2 of the subpathway that synthesizes L-tryptophan from chorismate.UniRule annotationProteins known to be involved in the 5 steps of the subpathway in this organism are:
- Anthranilate synthase component 1 (trpE), Anthranilate synthase component 2 (trpG)
- Anthranilate phosphoribosyltransferase (trpD)
- Phosphoribosyl isomerase A (priA)
- Indole-3-glycerol phosphate synthase (trpC)
- Tryptophan synthase beta chain (trpB), Tryptophan synthase alpha chain (trpA)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-tryptophan from chorismate, the pathway L-tryptophan biosynthesis and in Amino-acid biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 107 | Anthranilate 1; via carbonyl oxygenUniRule annotation | 1 | |
Binding sitei | 107 | Phosphoribosylpyrophosphate; via amide nitrogenUniRule annotation4 Publications | 1 | |
Binding sitei | 115 | PhosphoribosylpyrophosphateUniRule annotation4 Publications | 1 | |
Metal bindingi | 119 | Magnesium 1 | 1 | |
Binding sitei | 138 | Anthranilate 1 | 1 | |
Binding sitei | 147 | Phosphoribosylpyrophosphate; via amide nitrogenUniRule annotation4 Publications | 1 | |
Binding sitei | 193 | Anthranilate 2 | 1 | |
Metal bindingi | 251 | Magnesium 2 | 1 | |
Metal bindingi | 252 | Magnesium 1 | 1 | |
Metal bindingi | 252 | Magnesium 2 | 1 |
GO - Molecular functioni
- anthranilate phosphoribosyltransferase activity Source: UniProtKB-UniRule
- magnesium ion binding Source: UniProtKB-UniRule
GO - Biological processi
- tryptophan biosynthetic process Source: GO_Central
Keywordsi
Molecular function | Glycosyltransferase, Transferase |
Biological process | Amino-acid biosynthesis, Aromatic amino acid biosynthesis, Tryptophan biosynthesis |
Ligand | Magnesium, Metal-binding |
Enzyme and pathway databases
UniPathwayi | UPA00035;UER00041 |
Names & Taxonomyi
Protein namesi | Recommended name: Anthranilate phosphoribosyltransferaseUniRule annotation (EC:2.4.2.18UniRule annotation) |
Gene namesi | Name:trpDUniRule annotation Ordered Locus Names:Rv2192c ORF Names:MTCY190.03c |
Organismi | Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) |
Taxonomic identifieri | 83332 [NCBI] |
Taxonomic lineagei | Bacteria › Actinobacteria › Corynebacteriales › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex › |
Proteomesi |
|
Organism-specific databases
TubercuListi | Rv2192c |
Subcellular locationi
GO - Cellular componenti
- cytosol Source: GO_Central
- extracellular region Source: MTBBASE
- plasma membrane Source: MTBBASE
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedCombined sources | |||
ChainiPRO_0000154461 | 2 – 370 | Anthranilate phosphoribosyltransferaseAdd BLAST | 369 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylalanineCombined sources | 1 |
Keywords - PTMi
AcetylationProteomic databases
PaxDbi | P9WFX5 |
PTM databases
iPTMneti | P9WFX5 |
Interactioni
Subunit structurei
Homodimer.
UniRule annotation4 PublicationsProtein-protein interaction databases
STRINGi | 83332.Rv2192c |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P9WFX5 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 110 – 111 | Phosphoribosylpyrophosphate binding | 2 | |
Regioni | 117 – 120 | Phosphoribosylpyrophosphate binding | 4 | |
Regioni | 135 – 143 | Phosphoribosylpyrophosphate binding | 9 |
Sequence similaritiesi
Belongs to the anthranilate phosphoribosyltransferase family.UniRule annotation
Phylogenomic databases
eggNOGi | COG0547, Bacteria |
OMAi | LYHQSFK |
PhylomeDBi | P9WFX5 |
Family and domain databases
Gene3Di | 1.20.970.10, 1 hit 3.40.1030.10, 1 hit |
HAMAPi | MF_00211, TrpD, 1 hit |
InterProi | View protein in InterPro IPR005940, Anthranilate_Pribosyl_Tfrase IPR000312, Glycosyl_Trfase_fam3 IPR017459, Glycosyl_Trfase_fam3_N_dom IPR036320, Glycosyl_Trfase_fam3_N_dom)sf IPR035902, Nuc_phospho_transferase |
Pfami | View protein in Pfam PF02885, Glycos_trans_3N, 1 hit PF00591, Glycos_transf_3, 1 hit |
SUPFAMi | SSF47648, SSF47648, 1 hit SSF52418, SSF52418, 1 hit |
TIGRFAMsi | TIGR01245, trpD, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P9WFX5-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MALSAEGSSG GSRGGSPKAE AASVPSWPQI LGRLTDNRDL ARGQAAWAMD
60 70 80 90 100
QIMTGNARPA QIAAFAVAMT MKAPTADEVG ELAGVMLSHA HPLPADTVPD
110 120 130 140 150
DAVDVVGTGG DGVNTVNLST MAAIVVAAAG VPVVKHGNRA ASSLSGGADT
160 170 180 190 200
LEALGVRIDL GPDLVARSLA EVGIGFCFAP RFHPSYRHAA AVRREIGVPT
210 220 230 240 250
VFNLLGPLTN PARPRAGLIG CAFADLAEVM AGVFAARRSS VLVVHGDDGL
260 270 280 290 300
DELTTTTTST IWRVAAGSVD KLTFDPAGFG FARAQLDQLA GGDAQANAAA
310 320 330 340 350
VRAVLGGARG PVRDAVVLNA AGAIVAHAGL SSRAEWLPAW EEGLRRASAA
360 370
IDTGAAEQLL ARWVRFGRQI
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL123456 Genomic DNA Translation: CCP44969.1 |
PIRi | A70784 |
RefSeqi | NP_216708.1, NC_000962.3 WP_003411387.1, NZ_KK339370.1 |
Genome annotation databases
EnsemblBacteriai | CCP44969; CCP44969; Rv2192c |
GeneIDi | 887681 |
KEGGi | mtu:Rv2192c |
PATRICi | fig|83332.111.peg.2439 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL123456 Genomic DNA Translation: CCP44969.1 |
PIRi | A70784 |
RefSeqi | NP_216708.1, NC_000962.3 WP_003411387.1, NZ_KK339370.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1ZVW | X-ray | 2.30 | A/B | 1-370 | [»] | |
2BPQ | X-ray | 1.90 | A/B | 2-370 | [»] | |
3QQS | X-ray | 1.97 | A/B/C/D | 2-370 | [»] | |
3QR9 | X-ray | 1.87 | A/B | 2-370 | [»] | |
3QS8 | X-ray | 2.00 | A/B/C/D | 2-370 | [»] | |
3QSA | X-ray | 2.18 | A/B | 2-370 | [»] | |
3R6C | X-ray | 1.83 | A/B | 2-370 | [»] | |
3R88 | X-ray | 1.73 | A/B | 2-370 | [»] | |
3TWP | X-ray | 1.83 | A/B/C/D | 2-370 | [»] | |
3UU1 | X-ray | 1.82 | A/B/C/D | 2-370 | [»] | |
4GIU | X-ray | 1.67 | A/B | 1-370 | [»] | |
4GKM | X-ray | 1.67 | A/B | 2-370 | [»] | |
4IJ1 | X-ray | 1.79 | A/B | 2-370 | [»] | |
4M0R | X-ray | 1.96 | A/B | 2-370 | [»] | |
4N5V | X-ray | 1.90 | A/B | 1-370 | [»] | |
4N8Q | X-ray | 2.08 | A/B | 1-370 | [»] | |
4N93 | X-ray | 2.03 | A/B | 1-370 | [»] | |
4OWM | X-ray | 1.99 | A/B | 1-370 | [»] | |
4OWN | X-ray | 2.11 | A/B | 1-370 | [»] | |
4OWO | X-ray | 1.99 | A/B | 1-370 | [»] | |
4OWQ | X-ray | 1.89 | A/B | 1-370 | [»] | |
4OWS | X-ray | 2.43 | A/B | 1-370 | [»] | |
4OWU | X-ray | 1.89 | A/B | 1-370 | [»] | |
4OWV | X-ray | 1.90 | A/B | 1-370 | [»] | |
4X58 | X-ray | 1.75 | A/B | 1-370 | [»] | |
4X59 | X-ray | 1.80 | A/B | 1-370 | [»] | |
4X5A | X-ray | 1.93 | A/B | 1-370 | [»] | |
4X5B | X-ray | 2.47 | A/B | 1-370 | [»] | |
4X5C | X-ray | 2.33 | A/B | 1-370 | [»] | |
4X5D | X-ray | 2.30 | A/B | 1-370 | [»] | |
4X5E | X-ray | 1.77 | A/B | 1-370 | [»] | |
5BNE | X-ray | 2.15 | A/B/C/D | 2-370 | [»] | |
5BYT | X-ray | 2.00 | A/B | 2-370 | [»] | |
5C1R | X-ray | 1.80 | A/B | 2-370 | [»] | |
5C2L | X-ray | 2.01 | A/B | 2-370 | [»] | |
5C7S | X-ray | 2.10 | A/B | 2-370 | [»] | |
SMRi | P9WFX5 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
STRINGi | 83332.Rv2192c |
PTM databases
iPTMneti | P9WFX5 |
Proteomic databases
PaxDbi | P9WFX5 |
Genome annotation databases
EnsemblBacteriai | CCP44969; CCP44969; Rv2192c |
GeneIDi | 887681 |
KEGGi | mtu:Rv2192c |
PATRICi | fig|83332.111.peg.2439 |
Organism-specific databases
TubercuListi | Rv2192c |
Phylogenomic databases
eggNOGi | COG0547, Bacteria |
OMAi | LYHQSFK |
PhylomeDBi | P9WFX5 |
Enzyme and pathway databases
UniPathwayi | UPA00035;UER00041 |
Family and domain databases
Gene3Di | 1.20.970.10, 1 hit 3.40.1030.10, 1 hit |
HAMAPi | MF_00211, TrpD, 1 hit |
InterProi | View protein in InterPro IPR005940, Anthranilate_Pribosyl_Tfrase IPR000312, Glycosyl_Trfase_fam3 IPR017459, Glycosyl_Trfase_fam3_N_dom IPR036320, Glycosyl_Trfase_fam3_N_dom)sf IPR035902, Nuc_phospho_transferase |
Pfami | View protein in Pfam PF02885, Glycos_trans_3N, 1 hit PF00591, Glycos_transf_3, 1 hit |
SUPFAMi | SSF47648, SSF47648, 1 hit SSF52418, SSF52418, 1 hit |
TIGRFAMsi | TIGR01245, trpD, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | TRPD_MYCTU | |
Accessioni | P9WFX5Primary (citable) accession number: P9WFX5 Secondary accession number(s): L0T8W2, P66992, Q10382 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 16, 2014 |
Last sequence update: | April 16, 2014 | |
Last modified: | February 10, 2021 | |
This is version 45 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families