UniProtKB - P9WFF6 (DPDS_MYCTO)
Protein
Decaprenyl diphosphate synthase
Gene
uppS
Organism
Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh)
Status
Functioni
Catalyzes the sequential condensation of isopentenyl diphosphate (IPP) in the cis configuration with (2Z,6E)-farnesyl diphosphate (Z-FPP or EZ-FPP) generating the 50 carbon product trans,polycis-decaprenyl diphosphate.
Catalytic activityi
- (2Z,6E)-farnesyl diphosphate + 7 isopentenyl diphosphate = (2Z,6Z,10Z,14Z,18Z,22Z,26Z,30Z,34E)-decaprenyl diphosphate + 7 diphosphateEC:2.5.1.86
- (2E,6E)-farnesyl diphosphate + n isopentenyl diphosphate = di-trans,poly-cis-polyprenyl diphosphate + n diphosphateEC:2.5.1.87
Cofactori
Mg2+By similarityNote: Binds 2 magnesium ions per subunit.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 76 | By similarity | 1 | |
Metal bindingi | 76 | MagnesiumBy similarity | 1 | |
Binding sitei | 81 | SubstrateBy similarity | 1 | |
Binding sitei | 89 | SubstrateBy similarity | 1 | |
Binding sitei | 93 | SubstrateBy similarity | 1 | |
Active sitei | 124 | Proton acceptorBy similarity | 1 | |
Binding sitei | 125 | SubstrateBy similarity | 1 | |
Binding sitei | 127 | SubstrateBy similarity | 1 | |
Binding sitei | 168 | SubstrateBy similarity | 1 | |
Binding sitei | 244 | SubstrateBy similarity | 1 | |
Metal bindingi | 263 | MagnesiumBy similarity | 1 |
GO - Molecular functioni
- magnesium ion binding Source: UniProtKB-UniRule
- prenyltransferase activity Source: UniProtKB-UniRule
Keywordsi
Molecular function | Transferase |
Ligand | Magnesium, Metal-binding |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:uppS Ordered Locus Names:MT2430 |
Organismi | Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh) |
Taxonomic identifieri | 83331 [NCBI] |
Taxonomic lineagei | Bacteria › Actinobacteria › Corynebacteriales › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex › |
Proteomesi |
|
Subcellular locationi
- Cell membrane By similarity
GO - Cellular componenti
- plasma membrane Source: UniProtKB-SubCell
Keywords - Cellular componenti
Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000428547 | 1 – 296 | Decaprenyl diphosphate synthaseAdd BLAST | 296 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 76 – 80 | Substrate bindingBy similarity | 5 | |
Regioni | 121 – 124 | Substrate bindingBy similarity | 4 | |
Regioni | 250 – 252 | Substrate bindingBy similarity | 3 | |
Regioni | 292 – 294 | Substrate bindingBy similarity | 3 |
Sequence similaritiesi
Belongs to the UPP synthase family.Curated
Phylogenomic databases
HOGENOMi | CLU_038505_1_2_11 |
Family and domain databases
CDDi | cd00475, Cis_IPPS, 1 hit |
Gene3Di | 3.40.1180.10, 1 hit |
HAMAPi | MF_01139, ISPT, 1 hit |
InterProi | View protein in InterPro IPR001441, UPP_synth-like IPR018520, UPP_synth-like_CS IPR036424, UPP_synth-like_sf |
PANTHERi | PTHR10291, PTHR10291, 1 hit |
Pfami | View protein in Pfam PF01255, Prenyltransf, 1 hit |
SUPFAMi | SSF64005, SSF64005, 1 hit |
TIGRFAMsi | TIGR00055, uppS, 1 hit |
PROSITEi | View protein in PROSITE PS01066, UPP_SYNTHASE, 1 hit |
i Sequence
Sequence statusi: Complete.
P9WFF6-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MARDARKRTS SNFPQLPPAP DDYPTFPDTS TWPVVFPELP AAPYGGPCRP
60 70 80 90 100
PQHTSKAAAP RIPADRLPNH VAIVMDGNGR WATQRGLART EGHKMGEAVV
110 120 130 140 150
IDIACGAIEL GIKWLSLYAF STENWKRSPE EVRFLMGFNR DVVRRRRDTL
160 170 180 190 200
KKLGVRIRWV GSRPRLWRSV INELAVAEEM TKSNDVITIN YCVNYGGRTE
210 220 230 240 250
ITEATREIAR EVAAGRLNPE RITESTIARH LQRPDIPDVD LFLRTSGEQR
260 270 280 290
SSNFMLWQAA YAEYIFQDKL WPDYDRRDLW AACEEYASRT RRFGSA
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE000516 Genomic DNA Translation: AAK46724.1 |
PIRi | H70585 |
RefSeqi | WP_003412212.1, NZ_KK341227.1 |
Genome annotation databases
EnsemblBacteriai | AAK46724; AAK46724; MT2430 |
KEGGi | mtc:MT2430 |
PATRICi | fig|83331.31.peg.2618 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE000516 Genomic DNA Translation: AAK46724.1 |
PIRi | H70585 |
RefSeqi | WP_003412212.1, NZ_KK341227.1 |
3D structure databases
SMRi | P9WFF6 |
ModBasei | Search... |
Chemistry databases
BindingDBi | P9WFF6 |
Genome annotation databases
EnsemblBacteriai | AAK46724; AAK46724; MT2430 |
KEGGi | mtc:MT2430 |
PATRICi | fig|83331.31.peg.2618 |
Phylogenomic databases
HOGENOMi | CLU_038505_1_2_11 |
Family and domain databases
CDDi | cd00475, Cis_IPPS, 1 hit |
Gene3Di | 3.40.1180.10, 1 hit |
HAMAPi | MF_01139, ISPT, 1 hit |
InterProi | View protein in InterPro IPR001441, UPP_synth-like IPR018520, UPP_synth-like_CS IPR036424, UPP_synth-like_sf |
PANTHERi | PTHR10291, PTHR10291, 1 hit |
Pfami | View protein in Pfam PF01255, Prenyltransf, 1 hit |
SUPFAMi | SSF64005, SSF64005, 1 hit |
TIGRFAMsi | TIGR00055, uppS, 1 hit |
PROSITEi | View protein in PROSITE PS01066, UPP_SYNTHASE, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | DPDS_MYCTO | |
Accessioni | P9WFF6Primary (citable) accession number: P9WFF6 Secondary accession number(s): L0T9E4, O05837, P60479 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 16, 2014 |
Last sequence update: | April 16, 2014 | |
Last modified: | February 10, 2021 | |
This is version 33 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |