UniProtKB - P9WF10 (NNR_MYCTO)
Protein
Bifunctional NAD(P)H-hydrate repair enzyme Nnr
Gene
nnr
Organism
Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh)
Status
Functioni
Bifunctional enzyme that catalyzes the epimerization of the S- and R-forms of NAD(P)HX and the dehydration of the S-form of NAD(P)HX at the expense of ADP, which is converted to AMP. This allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration (By similarity).By similarity
Catalytic activityi
Cofactori
K+By similarityNote: Binds 1 potassium ion per subunit.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 63 | PotassiumBy similarity | 1 | |
Metal bindingi | 119 | PotassiumBy similarity | 1 | |
Binding sitei | 152 | NAD(P)HX (for epimerase activity)By similarity | 1 | |
Metal bindingi | 155 | PotassiumBy similarity | 1 | |
Binding sitei | 298 | NAD(P)HX (for dehydratase activity); via amide nitrogenBy similarity | 1 | |
Binding sitei | 412 | NAD(P)HX (for dehydratase activity)By similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 382 – 386 | ADPBy similarity | 5 | |
Nucleotide bindingi | 402 – 411 | ADPBy similarity | 10 |
GO - Molecular functioni
- ADP-dependent NAD(P)H-hydrate dehydratase activity Source: UniProtKB-UniRule
- ATP binding Source: UniProtKB-KW
- metal ion binding Source: UniProtKB-KW
- NADHX epimerase activity Source: UniProtKB-UniRule
- NADPHX epimerase activity Source: UniProtKB-EC
GO - Biological processi
- nicotinamide nucleotide metabolic process Source: UniProtKB-UniRule
Keywordsi
Molecular function | Isomerase, Lyase, Multifunctional enzyme |
Ligand | ATP-binding, Metal-binding, NAD, NADP, Nucleotide-binding, Potassium |
Names & Taxonomyi
Protein namesi | Recommended name: Bifunctional NAD(P)H-hydrate repair enzyme NnrAlternative name(s): Nicotinamide nucleotide repair protein Including the following 2 domains: |
Gene namesi | Name:nnr Ordered Locus Names:MT3539 |
Organismi | Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh) |
Taxonomic identifieri | 83331 [NCBI] |
Taxonomic lineagei | Bacteria › Actinobacteria › Corynebacteriales › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000428620 | 1 – 473 | Bifunctional NAD(P)H-hydrate repair enzyme NnrAdd BLAST | 473 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 10 – 205 | YjeF N-terminalAdd BLAST | 196 | |
Domaini | 210 – 473 | YjeF C-terminalAdd BLAST | 264 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 211 | NAD(P)H-hydrate epimeraseBy similarityAdd BLAST | 211 | |
Regioni | 62 – 66 | NAD(P)HX (for epimerase activity)By similarity | 5 | |
Regioni | 123 – 129 | NAD(P)HX (for epimerase activity)By similarity | 7 | |
Regioni | 211 – 473 | ADP-dependent (S)-NAD(P)H-hydrate dehydrataseBy similarityAdd BLAST | 263 | |
Regioni | 348 – 354 | NAD(P)HX (for dehydratase activity)By similarity | 7 |
Sequence similaritiesi
In the N-terminal section; belongs to the NnrE/AIBP family.Curated
In the C-terminal section; belongs to the NnrD/CARKD family.Curated
Phylogenomic databases
HOGENOMi | CLU_024853_4_0_11 |
Family and domain databases
CDDi | cd01171, YXKO-related, 1 hit |
Gene3Di | 3.40.1190.20, 1 hit 3.40.50.10260, 1 hit |
HAMAPi | MF_01965, NADHX_dehydratase, 1 hit MF_01966, NADHX_epimerase, 1 hit |
InterProi | View protein in InterPro IPR017953, Carbohydrate_kinase_pred_CS IPR000631, CARKD IPR030677, Nnr IPR029056, Ribokinase-like IPR004443, YjeF_N_dom IPR036652, YjeF_N_dom_sf |
Pfami | View protein in Pfam PF01256, Carb_kinase, 1 hit PF03853, YjeF_N, 1 hit |
PIRSFi | PIRSF017184, Nnr, 1 hit |
SUPFAMi | SSF53613, SSF53613, 1 hit SSF64153, SSF64153, 1 hit |
TIGRFAMsi | TIGR00196, yjeF_cterm, 1 hit TIGR00197, yjeF_nterm, 1 hit |
PROSITEi | View protein in PROSITE PS01049, YJEF_C_1, 1 hit PS01050, YJEF_C_2, 1 hit PS51383, YJEF_C_3, 1 hit PS51385, YJEF_N, 1 hit |
i Sequence
Sequence statusi: Complete.
P9WF10-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MRHYYSVDTI RAAEAPLLAS LPDGALMRRA AFGLATEIGR ELTARTGGVV
60 70 80 90 100
GRRVCAVVGS GDNGGDALWA ATFLRRRGAA ADAVLLNPDR THRKALAAFT
110 120 130 140 150
KSGGRLVESV SAATDLVIDG VVGISGSGPL RPAAAQVFAA VQAAAIPVVA
160 170 180 190 200
VDIPSGIDVA TGAITGPAVH AALTVTFGGL KPVHALADCG RVVLVDIGLD
210 220 230 240 250
LAHTDVLGFE ATDVAARWPV PGPRDDKYTQ GVTGVLAGSS TYPGAAVLCT
260 270 280 290 300
GAAVAATSGM VRYAGTAHAE VLAHWPEVIA SPTPAAAGRV QAWVVGPGLG
310 320 330 340 350
TDEAGAAALW FALDTDLPVL VDADGLTMLA DHPDLVAGRN APTVLTPHAG
360 370 380 390 400
EFARLAGAPP GDDRVGACRQ LADALGATVL LKGNVTVIAD PGGPVYLNPA
410 420 430 440 450
GQSWAATAGS GDVLSGMIGA LLASGLPSGE AAAAAAFVHA RASAAAAADP
460 470
GPGDAPTSAS RISGHIRAAL AAL
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE000516 Genomic DNA Translation: AAK47879.1 |
PIRi | G70975 |
RefSeqi | WP_003912231.1, NZ_KK341227.1 |
Genome annotation databases
EnsemblBacteriai | AAK47879; AAK47879; MT3539 |
KEGGi | mtc:MT3539 |
PATRICi | fig|83331.31.peg.3796 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE000516 Genomic DNA Translation: AAK47879.1 |
PIRi | G70975 |
RefSeqi | WP_003912231.1, NZ_KK341227.1 |
3D structure databases
SMRi | P9WF10 |
ModBasei | Search... |
Genome annotation databases
EnsemblBacteriai | AAK47879; AAK47879; MT3539 |
KEGGi | mtc:MT3539 |
PATRICi | fig|83331.31.peg.3796 |
Phylogenomic databases
HOGENOMi | CLU_024853_4_0_11 |
Family and domain databases
CDDi | cd01171, YXKO-related, 1 hit |
Gene3Di | 3.40.1190.20, 1 hit 3.40.50.10260, 1 hit |
HAMAPi | MF_01965, NADHX_dehydratase, 1 hit MF_01966, NADHX_epimerase, 1 hit |
InterProi | View protein in InterPro IPR017953, Carbohydrate_kinase_pred_CS IPR000631, CARKD IPR030677, Nnr IPR029056, Ribokinase-like IPR004443, YjeF_N_dom IPR036652, YjeF_N_dom_sf |
Pfami | View protein in Pfam PF01256, Carb_kinase, 1 hit PF03853, YjeF_N, 1 hit |
PIRSFi | PIRSF017184, Nnr, 1 hit |
SUPFAMi | SSF53613, SSF53613, 1 hit SSF64153, SSF64153, 1 hit |
TIGRFAMsi | TIGR00196, yjeF_cterm, 1 hit TIGR00197, yjeF_nterm, 1 hit |
PROSITEi | View protein in PROSITE PS01049, YJEF_C_1, 1 hit PS01050, YJEF_C_2, 1 hit PS51383, YJEF_C_3, 1 hit PS51385, YJEF_N, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | NNR_MYCTO | |
Accessioni | P9WF10Primary (citable) accession number: P9WF10 Secondary accession number(s): L0TFL5, O06250 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 16, 2014 |
Last sequence update: | April 16, 2014 | |
Last modified: | February 10, 2021 | |
This is version 37 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |