Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
We are aiming to switch to the new UniProt website on Monday, May 9. Please explore and share your feedback. Take me to the new website.

UniProtKB - P9WEW2 (STS1_STEHR)

Entry version 5 (23 Feb 2022)
Sequence version 1 (07 Oct 2020)
Previous versions | rss
Add a publicationFeedback
Protein

Delta(6)-protoilludene synthase STEHIDRAFT_73029

Gene

STEHIDRAFT_73029

Organism
Stereum hirsutum (strain FP-91666) (White-rot fungus)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Terpene cyclase that catalyzes the cyclization of farnesyl diphosphate (FPP) to delta6-protoilludene (PubMed:24166732, PubMed:26239156).

In presence of Ca2+, a significant switch from 1,11 to a dual 1,11/1,10 cyclization occurs, producing beta-elemene as the major product, with lower levels of delta6-protoilludene and (E)-beta-caryophyllene, and traces of beta-selinene and alpha-selinene (PubMed:26239156).

2 Publications

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the 'Function' section provides information relevant to cofactors. A cofactor is any non-protein substance required for a protein to be catalytically active. Some cofactors are inorganic, such as the metal atoms zinc, iron, and copper in various oxidation states. Others, such as most vitamins, are organic.<p><a href='/help/cofactor' target='_top'>More...</a></p>Cofactori

Mg2+1 Publication, Mn2+1 Publication, Ca2+1 Publication, Ni2+1 Publication, Co2+1 PublicationNote: Mg2+ and Mn2+ are more tightly associated with the active site motifs (D(D/E)XX(D/E) and NSE) and thus lead to more efficient removal of PPi (PubMed:26239156). In the presence of Co2+ and Ni2+ the activity is retained and delta(6)-protoilludene is the major volatile sesquiterpene produced (PubMed:26239156). In the presence of Ca2+, the major sesquiterpene produced switches to beta-elemene (PubMed:26239156).1 Publication

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes regulatory mechanisms for enzymes, transporters or microbial transcription factors, and reports the components which regulate (by activation or inhibition) the reaction.<p><a href='/help/activity_regulation' target='_top'>More...</a></p>Activity regulationi

Ca2+ switches the cyclization mechanism of delta6-protoilludene synthase from 1,11 to 1,10 cyclization which leads to the production of beta-elemene.1 Publication

<p>This subsection of the 'Function' section describes biophysical and chemical properties, such as maximal absorption, kinetic parameters, pH dependence, redox potentials and temperature dependence.<p><a href='/help/biophysicochemical_properties' target='_top'>More...</a></p>Kineticsi

  1. KM=1.52 µM for farnesyl diphosphate (FPP)1 Publication
  2. KM=33.5 µM for Mg2+1 Publication
  3. KM=8.5 µM for Mn2+1 Publication
  4. KM=12.9 µM for Ca2+1 Publication
  5. KM=16.0 µM for Ni2+1 Publication
  6. KM=30.3 µM for Co2+1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection describes interesting single amino acid sites on the sequence that are not defined in any other subsection. This subsection can be displayed in different sections ('Function', 'PTM / Processing', 'Pathology and Biotech') according to its content.<p><a href='/help/site' target='_top'>More...</a></p>Sitei104Plays a critical role in the stabilization of intermediate cationBy similarity1
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section indicates at which position the protein binds a given metal ion. The nature of the metal is indicated in the 'Description' field.<p><a href='/help/metal' target='_top'>More...</a></p>Metal bindingi107Magnesium 1By similarity1
Metal bindingi107Magnesium 2By similarity1
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the interaction between a single amino acid and another chemical entity. Priority is given to the annotation of physiological ligands.<p><a href='/help/binding' target='_top'>More...</a></p>Binding sitei199SubstrateBy similarity1
Metal bindingi243Magnesium 3By similarity1
Metal bindingi247Magnesium 3By similarity1
Metal bindingi251Magnesium 3By similarity1

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

Complete GO annotation on QuickGO ...

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionLyase
LigandCalcium, Cobalt, Magnesium, Manganese, Metal-binding, Nickel

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Delta(6)-protoilludene synthase STEHIDRAFT_730291 Publication (EC:4.2.3.-1 Publication, EC:4.2.3.1352 Publications, EC:4.2.3.1981 Publication)
Alternative name(s):
Terpene cyclase STEHIDRAFT_730291 Publication
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
ORF Names:STEHIDRAFT_73029
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiStereum hirsutum (strain FP-91666) (White-rot fungus)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri721885 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryota › Fungi › Dikarya › Basidiomycota › Agaricomycotina › Agaricomycetes › Russulales › Stereaceae › Stereum ›
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000053927 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Unassembled WGS sequence

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/manual/pathology%5Fand%5Fbiotech%5Fsection">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi53E → A: Does not affect cyclization in the presence of Mg(2+) or Ca(2+), but impairs the activity in the presence of Ni(2+) or Co(2+). 1 Publication1
Mutagenesisi111D → A: Does not drastically affect cyclization in the presence of Mg(2+), but impairs the activity in the presence of Ca(2+). 1 Publication1
Mutagenesisi116E → A: Does not drastically affect cyclization in the presence of Mg(2+) or Co(2+), but severely affects the activity in the presence of Ca(2+) and Ni(2+). 1 Publication1
Mutagenesisi117E → A: Does not drastically affect cyclization in the presence of Mg(2+) or Co(2+), but severely affects the activity in the presence of Ca(2+) and Ni(2+). 1 Publication1
Mutagenesisi182E → A: Does not drastically affect cyclization in the presence of Mg(2+), but severely affects the activity in the presence of Ca(2+), Ni(2+) or Co(2+). 1 Publication1

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00004512541 – 369Delta(6)-protoilludene synthase STEHIDRAFT_73029Add BLAST369

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...SMRi		P9WEW2

Database of comparative protein structure models

More...ModBasei		Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni349 – 350Substrate-bindingBy similarity2

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes a short (usually not more than 20 amino acids) conserved sequence motif of biological significance.<p><a href='/help/motif' target='_top'>More...</a></p>Motifi107 – 111D(D/E)XX(D/E) motif1 Publication5
Motifi243 – 251NSE motif1 Publication9

<p>This subsection of the 'Family and domains' section provides general information on the biological role of a domain. The term 'domain' is intended here in its wide acceptation, it may be a structural domain, a transmembrane region or a functional domain. Several domains are described in this subsection.<p><a href='/help/domain_cc' target='_top'>More...</a></p>Domaini

The 2 conserved active-site motifs D(D/E)XX(D/E) and NSE are required for coordinating the divalent metal ions that stabilize the PPi moiety of the substrate.1 Publication

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the terpene synthase family.Curated

Phylogenomic databases

Identification of Orthologs from Complete Genome Data

More...OMAi		ANIIMEA

Family and domain databases

Gene3D Structural and Functional Annotation of Protein Families

More...Gene3Di		1.10.600.10, 1 hit

Integrated resource of protein families, domains and functional sites

More...InterProi		View protein in InterPro
IPR008949, Isoprenoid_synthase_dom_sf
IPR034686, Terpene_cyclase-like_2

Structure-Function Linkage Database

More...SFLDi		SFLDG01020, Terpene_Cyclase_Like_2, 1 hit

Superfamily database of structural and functional annotation

More...SUPFAMi		SSF48576, SSF48576, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

P9WEW2-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAVATSVATP VPTPAYSAGR APAKEKKIYL PDTLAEWPWP RAINPHYAEA 
        60         70         80         90        100
KEESQAWAAS FNAFSPKAQH AFNRCDFNLL ASLAYPLATK HGCRSGCDLM 
       110        120        130        140        150
NLFFVIDEYS DIAPVEEVRQ QKDIVMDALR NPHKPRPEGE WVGGEVARQF 
       160        170        180        190        200
WALTITNASA QSQKHFIETF DEYLDSVVQQ AEDRSESRIR DIQSYIDVRR 
       210        220        230        240        250
NTIGAKPSFA LLELDMDLPD EVLAHPTIQS LSLATIDMLC LGNDIVSYNL 
       260        270        280        290        300
EQARGDASHN IITIVMNELN LDVNGAMRWV GDFHKQLEKQ FFEAFNNLPK 
       310        320        330        340        350
WGNAELDAQI AVYCDGLGNW VRANDQWSFE SERYFGARGL EIMETKTLAM 
       360 
MPIQRTEALG PQLVDDSIL
Length:369
Mass (Da):41,515
Last modified:October 7, 2020 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i0D88AC9A87349980
GO

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...EMBLi

GenBank nucleotide sequence database

More...GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...DDBJi
Links Updated
JH687380 Genomic DNA Translation: EIM91236.1

NCBI Reference Sequences

More...RefSeqi		XP_007299456.1, XM_007299394.1

Genome annotation databases

Database of genes from NCBI RefSeq genomes

More...GeneIDi		18806761

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...KEGGi		shs:STEHIDRAFT_73029

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
JH687380 Genomic DNA Translation: EIM91236.1
RefSeqiXP_007299456.1, XM_007299394.1

3D structure databases

SMRiP9WEW2
ModBaseiSearch...

Genome annotation databases

GeneIDi18806761
KEGGishs:STEHIDRAFT_73029

Phylogenomic databases

OMAiANIIMEA

Family and domain databases

Gene3Di1.10.600.10, 1 hit
InterProiView protein in InterPro
IPR008949, Isoprenoid_synthase_dom_sf
IPR034686, Terpene_cyclase-like_2
SFLDiSFLDG01020, Terpene_Cyclase_Like_2, 1 hit
SUPFAMiSSF48576, SSF48576, 1 hit

MobiDB: a database of protein disorder and mobility annotations

More...MobiDBi		Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiSTS1_STEHR
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P9WEW2
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 7, 2020
Last sequence update: October 7, 2020
Last modified: February 23, 2022
This is version 5 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again