UniProtKB - P99029 (PRDX5_MOUSE)
Protein
Peroxiredoxin-5, mitochondrial
Gene
Prdx5
Organism
Mus musculus (Mouse)
Status
Functioni
Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides and as sensor of hydrogen peroxide-mediated signaling events.1 Publication
Miscellaneous
The active site is a conserved redox-active cysteine residue, the peroxidatic cysteine (C(P)), which makes the nucleophilic attack on the peroxide substrate. The peroxide oxidizes the C(P)-SH to cysteine sulfenic acid (C(P)-SOH), which then reacts with another cysteine residue, the resolving cysteine (C(R)), to form a disulfide bridge. The disulfide is subsequently reduced by an appropriate electron donor to complete the catalytic cycle. In this atypical 2-Cys Prx, C(R) is present in the same subunit to form an intramolecular disulfide. The disulfide is subsequently reduced by thioredoxin.By similarity
Catalytic activityi
- EC:1.11.1.241 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 96 | Cysteine sulfenic acid (-SOH) intermediateBy similarity | 1 |
GO - Molecular functioni
- antioxidant activity Source: UniProtKB
- cysteine-type endopeptidase inhibitor activity involved in apoptotic process Source: MGI
- peroxidase activity Source: MGI
- peroxiredoxin activity Source: MGI
- peroxynitrite reductase activity Source: MGI
- RNA polymerase III transcription regulatory region sequence-specific DNA binding Source: MGI
- signaling receptor binding Source: UniProtKB
- thioredoxin peroxidase activity Source: MGI
GO - Biological processi
- cell redox homeostasis Source: GO_Central
- cellular response to oxidative stress Source: GO_Central
- cellular response to reactive oxygen species Source: MGI
- hydrogen peroxide catabolic process Source: UniProtKB
- NADPH oxidation Source: MGI
- negative regulation of apoptotic process Source: MGI
- negative regulation of oxidoreductase activity Source: MGI
- negative regulation of transcription by RNA polymerase III Source: MGI
- positive regulation of collagen biosynthetic process Source: MGI
- reactive nitrogen species metabolic process Source: MGI
- regulation of apoptosis involved in tissue homeostasis Source: MGI
- response to oxidative stress Source: UniProtKB
Keywordsi
Molecular function | Antioxidant, Oxidoreductase, Peroxidase |
Enzyme and pathway databases
Reactomei | R-MMU-3299685, Detoxification of Reactive Oxygen Species R-MMU-5628897, TP53 Regulates Metabolic Genes |
Protein family/group databases
PeroxiBasei | 4453, MmPrxV |
Names & Taxonomyi
Protein namesi | Recommended name: Peroxiredoxin-5, mitochondrial (EC:1.11.1.241 Publication)Alternative name(s): Antioxidant enzyme B166 Short name: AOEB166 Liver tissue 2D-page spot 2D-0014IV PLP Peroxiredoxin V Short name: Prx-V Peroxisomal antioxidant enzyme Thioredoxin peroxidase PMP20 Thioredoxin-dependent peroxiredoxin 5Curated |
Gene namesi | |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1859821, Prdx5 |
Subcellular locationi
Mitochondrion
- Mitochondrion By similarity
Peroxisome
- Peroxisome matrix By similarity
Other locations
- Cytoplasm By similarity
Cytosol
- cytosol Source: MGI
Mitochondrion
- mitochondrion Source: MGI
Nucleus
- nucleus Source: MGI
Peroxisome
- peroxisomal matrix Source: UniProtKB
- peroxisome Source: MGI
Other locations
- cytoplasm Source: UniProtKB
- cytoplasmic vesicle Source: MGI
- intracellular membrane-bounded organelle Source: MGI
- perinuclear region of cytoplasm Source: MGI
Keywords - Cellular componenti
Cytoplasm, Mitochondrion, PeroxisomePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – 48 | MitochondrionSequence analysisAdd BLAST | 48 | |
ChainiPRO_0000023795 | 49 – 210 | Peroxiredoxin-5, mitochondrialAdd BLAST | 162 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 70 | N6-acetyllysine; alternateCombined sources | 1 | |
Modified residuei | 70 | N6-succinyllysine; alternateCombined sources | 1 | |
Modified residuei | 71 | N6-acetyllysineCombined sources | 1 | |
Modified residuei | 79 | N6-acetyllysine; alternateCombined sources | 1 | |
Modified residuei | 79 | N6-succinyllysine; alternateCombined sources | 1 | |
Disulfide bondi | 96 ↔ 200 | Redox-activePROSITE-ProRule annotation | ||
Lipidationi | 96 | S-palmitoyl cysteineBy similarity | 1 | |
Modified residuei | 112 | N6-succinyllysineCombined sources | 1 | |
Modified residuei | 167 | PhosphoserineBy similarity | 1 | |
Modified residuei | 178 | PhosphoserineCombined sources | 1 |
Post-translational modificationi
S-palmitoylated. Palmitoylation occurs on the active site, inhibiting its reactivity; therefore PRDX5 palmitoylation status determines its antioxidant capacity.1 Publication
S-palmitoylated. Depalmitoylated by ABHD10.By similarity
Keywords - PTMi
Acetylation, Disulfide bond, Lipoprotein, Palmitate, PhosphoproteinProteomic databases
jPOSTi | P99029 |
MaxQBi | P99029 |
PaxDbi | P99029 |
PeptideAtlasi | P99029 |
PRIDEi | P99029 |
2D gel databases
REPRODUCTION-2DPAGEi | P99029 |
SWISS-2DPAGEi | P99029 |
PTM databases
iPTMneti | P99029 |
PhosphoSitePlusi | P99029 |
SwissPalmi | P99029 |
Expressioni
Tissue specificityi
Widely expressed.
Gene expression databases
Bgeei | ENSMUSG00000024953, Expressed in granulocyte and 322 other tissues |
ExpressionAtlasi | P99029, baseline and differential |
Genevisiblei | P99029, MM |
Interactioni
Subunit structurei
Monomer.
By similarityBinary interactionsi
P99029
With | #Exp. | IntAct |
---|---|---|
Sod1 [P08228] | 2 | EBI-2735704,EBI-1635090 |
GO - Molecular functioni
- signaling receptor binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 207718, 12 interactors |
IntActi | P99029, 7 interactors |
MINTi | P99029 |
STRINGi | 10090.ENSMUSP00000025904 |
Miscellaneous databases
RNActi | P99029, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 52 – 210 | ThioredoxinPROSITE-ProRule annotationAdd BLAST | 159 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 208 – 210 | Microbody targeting signalBy similarity | 3 |
Sequence similaritiesi
Keywords - Domaini
Redox-active center, Transit peptidePhylogenomic databases
eggNOGi | KOG0541, Eukaryota |
GeneTreei | ENSGT00390000018173 |
InParanoidi | P99029 |
OMAi | GYINHPK |
OrthoDBi | 1281610at2759 |
PhylomeDBi | P99029 |
TreeFami | TF105182 |
Family and domain databases
CDDi | cd03013, PRX5_like, 1 hit |
Gene3Di | 3.40.30.10, 1 hit |
InterProi | View protein in InterPro IPR037944, PRX5-like IPR013740, Redoxin IPR036249, Thioredoxin-like_sf IPR013766, Thioredoxin_domain |
PANTHERi | PTHR10430, PTHR10430, 1 hit |
Pfami | View protein in Pfam PF08534, Redoxin, 1 hit |
SUPFAMi | SSF52833, SSF52833, 1 hit |
PROSITEi | View protein in PROSITE PS51352, THIOREDOXIN_2, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative initiation. AlignAdd to basketThis entry has 2 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All
Isoform Mitochondrial (identifier: P99029-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MLQLGLRVLG CKASSVLRAS TCLAGRAGRK EAGWECGGAR SFSSSAVTMA
60 70 80 90 100
PIKVGDAIPS VEVFEGEPGK KVNLAELFKG KKGVLFGVPG AFTPGCSKTH
110 120 130 140 150
LPGFVEQAGA LKAKGAQVVA CLSVNDVFVI EEWGRAHQAE GKVRLLADPT
160 170 180 190 200
GAFGKATDLL LDDSLVSLFG NRRLKRFSMV IDNGIVKALN VEPDGTGLTC
210
SLAPNILSQL
Isoform Cytoplasmic+peroxisomal (identifier: P99029-2) [UniParc]FASTAAdd to basket
The sequence of this isoform differs from the canonical sequence as follows:
1-48: Missing.
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketH3BJQ7 | H3BJQ7_MOUSE | Peroxiredoxin-5 | Prdx5 | 213 | Annotation score: | ||
A0A494BAZ4 | A0A494BAZ4_MOUSE | Peroxiredoxin-5 | Prdx5 | 142 | Annotation score: | ||
G3UZJ4 | G3UZJ4_MOUSE | Thioredoxin-dependent peroxiredoxin | Prdx5 | 166 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 55 | G → D AA sequence (Ref. 7) Curated | 1 | |
Sequence conflicti | 83 – 102 | GVLFG…KTHLP → VFCLESLGHLHLAVLRPTA in AAF21016 (PubMed:10753630).CuratedAdd BLAST | 20 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_018830 | 1 – 48 | Missing in isoform Cytoplasmic+peroxisomal. CuratedAdd BLAST | 48 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF197951 mRNA Translation: AAF04855.1 AF124994 mRNA Translation: AAF27532.1 AF110733 mRNA Translation: AAG13450.1 AF208730, AF208729 Genomic DNA Translation: AAF21016.1 AK002383 mRNA Translation: BAB22058.1 AK003332 mRNA Translation: BAB22720.1 BC008174 mRNA Translation: AAH08174.1 |
CCDSi | CCDS29507.1 [P99029-1] |
PIRi | JC7239 |
RefSeqi | NP_036151.1, NM_012021.2 [P99029-1] |
Genome annotation databases
Ensembli | ENSMUST00000025904; ENSMUSP00000025904; ENSMUSG00000024953 [P99029-1] |
GeneIDi | 54683 |
KEGGi | mmu:54683 |
UCSCi | uc008gjc.1, mouse [P99029-1] |
Keywords - Coding sequence diversityi
Alternative initiationSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF197951 mRNA Translation: AAF04855.1 AF124994 mRNA Translation: AAF27532.1 AF110733 mRNA Translation: AAG13450.1 AF208730, AF208729 Genomic DNA Translation: AAF21016.1 AK002383 mRNA Translation: BAB22058.1 AK003332 mRNA Translation: BAB22720.1 BC008174 mRNA Translation: AAH08174.1 |
CCDSi | CCDS29507.1 [P99029-1] |
PIRi | JC7239 |
RefSeqi | NP_036151.1, NM_012021.2 [P99029-1] |
3D structure databases
SMRi | P99029 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 207718, 12 interactors |
IntActi | P99029, 7 interactors |
MINTi | P99029 |
STRINGi | 10090.ENSMUSP00000025904 |
Protein family/group databases
PeroxiBasei | 4453, MmPrxV |
PTM databases
iPTMneti | P99029 |
PhosphoSitePlusi | P99029 |
SwissPalmi | P99029 |
2D gel databases
REPRODUCTION-2DPAGEi | P99029 |
SWISS-2DPAGEi | P99029 |
Proteomic databases
jPOSTi | P99029 |
MaxQBi | P99029 |
PaxDbi | P99029 |
PeptideAtlasi | P99029 |
PRIDEi | P99029 |
Protocols and materials databases
Antibodypediai | 3276, 423 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000025904; ENSMUSP00000025904; ENSMUSG00000024953 [P99029-1] |
GeneIDi | 54683 |
KEGGi | mmu:54683 |
UCSCi | uc008gjc.1, mouse [P99029-1] |
Organism-specific databases
CTDi | 25824 |
MGIi | MGI:1859821, Prdx5 |
Phylogenomic databases
eggNOGi | KOG0541, Eukaryota |
GeneTreei | ENSGT00390000018173 |
InParanoidi | P99029 |
OMAi | GYINHPK |
OrthoDBi | 1281610at2759 |
PhylomeDBi | P99029 |
TreeFami | TF105182 |
Enzyme and pathway databases
Reactomei | R-MMU-3299685, Detoxification of Reactive Oxygen Species R-MMU-5628897, TP53 Regulates Metabolic Genes |
Miscellaneous databases
BioGRID-ORCSi | 54683, 1 hit in 17 CRISPR screens |
ChiTaRSi | Prdx5, mouse |
PROi | PR:P99029 |
RNActi | P99029, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000024953, Expressed in granulocyte and 322 other tissues |
ExpressionAtlasi | P99029, baseline and differential |
Genevisiblei | P99029, MM |
Family and domain databases
CDDi | cd03013, PRX5_like, 1 hit |
Gene3Di | 3.40.30.10, 1 hit |
InterProi | View protein in InterPro IPR037944, PRX5-like IPR013740, Redoxin IPR036249, Thioredoxin-like_sf IPR013766, Thioredoxin_domain |
PANTHERi | PTHR10430, PTHR10430, 1 hit |
Pfami | View protein in Pfam PF08534, Redoxin, 1 hit |
SUPFAMi | SSF52833, SSF52833, 1 hit |
PROSITEi | View protein in PROSITE PS51352, THIOREDOXIN_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PRDX5_MOUSE | |
Accessioni | P99029Primary (citable) accession number: P99029 Secondary accession number(s): Q9QX45, Q9QZ75 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 15, 1998 |
Last sequence update: | February 21, 2002 | |
Last modified: | December 2, 2020 | |
This is version 176 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families