UniProtKB - P98198 (AT8B2_HUMAN)
Phospholipid-transporting ATPase ID
ATP8B2
Functioni
Catalytic activityi
- ATP + H(2)O + phospholipid(Side 1) = ADP + phosphate + phospholipid(Side 2). EC:7.6.2.1
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 411 | 4-aspartylphosphate intermediateBy similarity | 1 | |
Metal bindingi | 833 | MagnesiumBy similarity | 1 | |
Metal bindingi | 837 | MagnesiumBy similarity | 1 |
GO - Molecular functioni
- ATPase-coupled intramembrane lipid transporter activity Source: GO_Central
- ATP binding Source: UniProtKB-KW
- magnesium ion binding Source: InterPro
GO - Biological processi
- Golgi organization Source: GO_Central
- ion transmembrane transport Source: Reactome
- phospholipid translocation Source: GO_Central
Keywordsi
Molecular function | Translocase |
Biological process | Lipid transport, Transport |
Ligand | ATP-binding, Magnesium, Metal-binding, Nucleotide-binding |
Enzyme and pathway databases
PathwayCommonsi | P98198 |
Reactomei | R-HSA-936837, Ion transport by P-type ATPases |
Names & Taxonomyi
Protein namesi | Recommended name: Phospholipid-transporting ATPase ID (EC:7.6.2.1)Alternative name(s): ATPase class I type 8B member 2 P4-ATPase flippase complex alpha subunit ATP8B2 |
Gene namesi | Name:ATP8B2 Synonyms:ATPID, KIAA1137 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:13534, ATP8B2 |
MIMi | 605867, gene |
neXtProti | NX_P98198 |
VEuPathDBi | HostDB:ENSG00000143515.16 |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum membrane 1 Publication; Multi-pass membrane protein Sequence analysis
Plasma membrane
- Cell membrane 2 Publications; Multi-pass membrane protein Sequence analysis
Note: TMEM30A, but not TMEM30B, is required for its export from endoplasmic reticulum to the plasma membrane.1 Publication
Endoplasmic reticulum
- endoplasmic reticulum membrane Source: UniProtKB-SubCell
Golgi apparatus
- Golgi apparatus Source: UniProtKB
- trans-Golgi network Source: GO_Central
Plasma Membrane
- plasma membrane Source: UniProtKB
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 64 | CytoplasmicSequence analysisAdd BLAST | 64 | |
Transmembranei | 65 – 86 | HelicalSequence analysisAdd BLAST | 22 | |
Topological domaini | 87 – 92 | Exoplasmic loopSequence analysis | 6 | |
Transmembranei | 93 – 112 | HelicalSequence analysisAdd BLAST | 20 | |
Topological domaini | 113 – 295 | CytoplasmicSequence analysisAdd BLAST | 183 | |
Transmembranei | 296 – 317 | HelicalSequence analysisAdd BLAST | 22 | |
Topological domaini | 318 – 346 | Exoplasmic loopSequence analysisAdd BLAST | 29 | |
Transmembranei | 347 – 368 | HelicalSequence analysisAdd BLAST | 22 | |
Topological domaini | 369 – 889 | CytoplasmicSequence analysisAdd BLAST | 521 | |
Transmembranei | 890 – 910 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 911 – 922 | Exoplasmic loopSequence analysisAdd BLAST | 12 | |
Transmembranei | 923 – 942 | HelicalSequence analysisAdd BLAST | 20 | |
Topological domaini | 943 – 972 | CytoplasmicSequence analysisAdd BLAST | 30 | |
Transmembranei | 973 – 994 | HelicalSequence analysisAdd BLAST | 22 | |
Topological domaini | 995 – 1008 | Exoplasmic loopSequence analysisAdd BLAST | 14 | |
Transmembranei | 1009 – 1031 | HelicalSequence analysisAdd BLAST | 23 | |
Topological domaini | 1032 – 1037 | CytoplasmicSequence analysis | 6 | |
Transmembranei | 1038 – 1058 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 1059 – 1078 | Exoplasmic loopSequence analysisAdd BLAST | 20 | |
Transmembranei | 1079 – 1103 | HelicalSequence analysisAdd BLAST | 25 | |
Topological domaini | 1104 – 1209 | CytoplasmicSequence analysisAdd BLAST | 106 |
Keywords - Cellular componenti
Cell membrane, Endoplasmic reticulum, MembranePathology & Biotechi
Organism-specific databases
DisGeNETi | 57198 |
OpenTargetsi | ENSG00000143515 |
PharmGKBi | PA25167 |
Miscellaneous databases
Pharosi | P98198, Tdark |
Genetic variation databases
BioMutai | ATP8B2 |
DMDMi | 30316371 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000046365 | 1 – 1209 | Phospholipid-transporting ATPase IDAdd BLAST | 1209 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1175 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | P98198 |
jPOSTi | P98198 |
MassIVEi | P98198 |
MaxQBi | P98198 |
PaxDbi | P98198 |
PeptideAtlasi | P98198 |
PRIDEi | P98198 |
ProteomicsDBi | 57827 [P98198-1] 57828 [P98198-2] 57829 [P98198-3] 57830 [P98198-4] |
PTM databases
iPTMneti | P98198 |
PhosphoSitePlusi | P98198 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSG00000143515, Expressed in thoracic aorta and 219 other tissues |
Genevisiblei | P98198, HS |
Organism-specific databases
HPAi | ENSG00000143515, Low tissue specificity |
Interactioni
Subunit structurei
Component of a P4-ATPase flippase complex which consists of a catalytic alpha subunit and an accessory beta subunit (Probable). The probable flippase ATP8B2:TMEM30A complex can form an intermediate phosphoenzyme in vitro. The interaction with TMEM30B is reported conflictingly.
CuratedProtein-protein interaction databases
BioGRIDi | 121445, 22 interactors |
IntActi | P98198, 15 interactors |
STRINGi | 9606.ENSP00000357475 |
Miscellaneous databases
RNActi | P98198, protein |
Family & Domainsi
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG0206, Eukaryota |
GeneTreei | ENSGT00940000160804 |
HOGENOMi | CLU_000846_8_4_1 |
InParanoidi | P98198 |
OMAi | RMENDQF |
PhylomeDBi | P98198 |
TreeFami | TF300654 |
Family and domain databases
Gene3Di | 3.40.1110.10, 2 hits 3.40.50.1000, 2 hits |
InterProi | View protein in InterPro IPR030347, ATP8B2 IPR023299, ATPase_P-typ_cyto_dom_N IPR018303, ATPase_P-typ_P_site IPR023298, ATPase_P-typ_TM_dom_sf IPR008250, ATPase_P-typ_transduc_dom_A_sf IPR036412, HAD-like_sf IPR023214, HAD_sf IPR006539, P-type_ATPase_IV IPR032631, P-type_ATPase_N IPR001757, P_typ_ATPase IPR032630, P_typ_ATPase_c |
PANTHERi | PTHR24092, PTHR24092, 1 hit PTHR24092:SF46, PTHR24092:SF46, 1 hit |
Pfami | View protein in Pfam PF16212, PhoLip_ATPase_C, 1 hit PF16209, PhoLip_ATPase_N, 1 hit |
SUPFAMi | SSF56784, SSF56784, 1 hit SSF81653, SSF81653, 1 hit SSF81660, SSF81660, 1 hit SSF81665, SSF81665, 1 hit |
TIGRFAMsi | TIGR01652, ATPase-Plipid, 1 hit TIGR01494, ATPase_P-type, 1 hit |
PROSITEi | View protein in PROSITE PS00154, ATPASE_E1_E2, 1 hit |
s (4+)i Sequence
Sequence statusi: Complete.
This entry describes 4 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 4 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MTVPKEMPEK WARAQAPPSW SRKKPSWGTE EERRARANDR EYNEKFQYAS
60 70 80 90 100
NCIKTSKYNI LTFLPVNLFE QFQEVANTYF LFLLILQLIP QISSLSWFTT
110 120 130 140 150
IVPLVLVLTI TAVKDATDDY FRHKSDNQVN NRQSQVLING ILQQEQWMNV
160 170 180 190 200
CVGDIIKLEN NQFVAADLLL LSSSEPHGLC YIETAELDGE TNMKVRQAIP
210 220 230 240 250
VTSELGDISK LAKFDGEVIC EPPNNKLDKF SGTLYWKENK FPLSNQNMLL
260 270 280 290 300
RGCVLRNTEW CFGLVIFAGP DTKLMQNSGR TKFKRTSIDR LMNTLVLWIF
310 320 330 340 350
GFLVCMGVIL AIGNAIWEHE VGMRFQVYLP WDEAVDSAFF SGFLSFWSYI
360 370 380 390 400
IILNTVVPIS LYVSVEVIRL GHSYFINWDK KMFCMKKRTP AEARTTTLNE
410 420 430 440 450
ELGQVEYIFS DKTGTLTQNI MVFNKCSING HSYGDVFDVL GHKAELGERP
460 470 480 490 500
EPVDFSFNPL ADKKFLFWDP SLLEAVKIGD PHTHEFFRLL SLCHTVMSEE
510 520 530 540 550
KNEGELYYKA QSPDEGALVT AARNFGFVFR SRTPKTITVH EMGTAITYQL
560 570 580 590 600
LAILDFNNIR KRMSVIVRNP EGKIRLYCKG ADTILLDRLH HSTQELLNTT
610 620 630 640 650
MDHLNEYAGE GLRTLVLAYK DLDEEYYEEW AERRLQASLA QDSREDRLAS
660 670 680 690 700
IYEEVENNMM LLGATAIEDK LQQGVPETIA LLTLANIKIW VLTGDKQETA
710 720 730 740 750
VNIGYSCKML TDDMTEVFIV TGHTVLEVRE ELRKAREKMM DSSRSVGNGF
760 770 780 790 800
TYQDKLSSSK LTSVLEAVAG EYALVINGHS LAHALEADME LEFLETACAC
810 820 830 840 850
KAVICCRVTP LQKAQVVELV KKYKKAVTLA IGDGANDVSM IKTAHIGVGI
860 870 880 890 900
SGQEGIQAVL ASDYSFSQFK FLQRLLLVHG RWSYLRMCKF LCYFFYKNFA
910 920 930 940 950
FTMVHFWFGF FCGFSAQTVY DQYFITLYNI VYTSLPVLAM GVFDQDVPEQ
960 970 980 990 1000
RSMEYPKLYE PGQLNLLFNK REFFICIAQG IYTSVLMFFI PYGVFADATR
1010 1020 1030 1040 1050
DDGTQLADYQ SFAVTVATSL VIVVSVQIGL DTGYWTAINH FFIWGSLAVY
1060 1070 1080 1090 1100
FAILFAMHSN GLFDMFPNQF RFVGNAQNTL AQPTVWLTIV LTTVVCIMPV
1110 1120 1130 1140 1150
VAFRFLRLNL KPDLSDTVRY TQLVRKKQKA QHRCMRRVGR TGSRRSGYAF
1160 1170 1180 1190 1200
SHQEGFGELI MSGKNMRLSS LALSSFTTRS SSSWIESLRR KKSDSASSPS
GGADKPLKG
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A5K1VW70 | A0A5K1VW70_HUMAN | Phospholipid-transporting ATPase | ATP8B2 | 1,190 | Annotation score: |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 834 | G → E in BAA86451 (PubMed:15489334).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_037147 | 1 – 29 | MTVPK…PSWGT → MDTLRAVPLFSISGLFSFPY RVSHGIAGILLGEMAVCAKK RPP in isoform 3. 1 PublicationAdd BLAST | 29 | |
Alternative sequenceiVSP_037148 | 1 – 29 | MTVPK…PSWGT → MAVCAKKRPP in isoform 4. 1 PublicationAdd BLAST | 29 | |
Alternative sequenceiVSP_037149 | 364 – 406 | SVEVI…LGQVE → RYVPSLTWGLSRESGGPIEL FFSMKMKSLRSNEKSSSSCT VNI in isoform 4. 1 PublicationAdd BLAST | 43 | |
Alternative sequenceiVSP_037150 | 407 – 1209 | Missing in isoform 4. 1 PublicationAdd BLAST | 803 | |
Alternative sequenceiVSP_007302 | 434 – 461 | GDVFD…FNPLA → VGSEGPRRKGPSWPGTVAHA YSHSTLGG in isoform 2. 1 PublicationAdd BLAST | 28 | |
Alternative sequenceiVSP_007303 | 462 – 1209 | Missing in isoform 2. 1 PublicationAdd BLAST | 748 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY302537 mRNA Translation: AAQ19027.1 AK054886 mRNA Translation: BAB70822.1 Frameshift. AK304806 mRNA Translation: BAG65556.1 Different initiation. AL162591 Genomic DNA No translation available. CH471121 Genomic DNA Translation: EAW53205.1 BC007837 mRNA Translation: AAH07837.2 BC030288 mRNA Translation: AAH30288.1 Different initiation. BC069264 mRNA Translation: AAH69264.1 AB032963 mRNA Translation: BAA86451.1 AL137537 mRNA Translation: CAB70799.1 |
CCDSi | CCDS41405.1 [P98198-4] |
PIRi | T46381 |
RefSeqi | NP_001005855.1, NM_001005855.1 [P98198-4] NP_065185.1, NM_020452.3 XP_005245412.1, XM_005245355.2 |
Genome annotation databases
Ensembli | ENST00000368487; ENSP00000357472; ENSG00000143515 [P98198-4] ENST00000672630; ENSP00000500034; ENSG00000143515 [P98198-3] |
GeneIDi | 57198 |
KEGGi | hsa:57198 |
UCSCi | uc001few.4, human [P98198-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY302537 mRNA Translation: AAQ19027.1 AK054886 mRNA Translation: BAB70822.1 Frameshift. AK304806 mRNA Translation: BAG65556.1 Different initiation. AL162591 Genomic DNA No translation available. CH471121 Genomic DNA Translation: EAW53205.1 BC007837 mRNA Translation: AAH07837.2 BC030288 mRNA Translation: AAH30288.1 Different initiation. BC069264 mRNA Translation: AAH69264.1 AB032963 mRNA Translation: BAA86451.1 AL137537 mRNA Translation: CAB70799.1 |
CCDSi | CCDS41405.1 [P98198-4] |
PIRi | T46381 |
RefSeqi | NP_001005855.1, NM_001005855.1 [P98198-4] NP_065185.1, NM_020452.3 XP_005245412.1, XM_005245355.2 |
3D structure databases
SMRi | P98198 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 121445, 22 interactors |
IntActi | P98198, 15 interactors |
STRINGi | 9606.ENSP00000357475 |
PTM databases
iPTMneti | P98198 |
PhosphoSitePlusi | P98198 |
Genetic variation databases
BioMutai | ATP8B2 |
DMDMi | 30316371 |
Proteomic databases
EPDi | P98198 |
jPOSTi | P98198 |
MassIVEi | P98198 |
MaxQBi | P98198 |
PaxDbi | P98198 |
PeptideAtlasi | P98198 |
PRIDEi | P98198 |
ProteomicsDBi | 57827 [P98198-1] 57828 [P98198-2] 57829 [P98198-3] 57830 [P98198-4] |
Protocols and materials databases
Antibodypediai | 34152, 91 antibodies |
DNASUi | 57198 |
Genome annotation databases
Ensembli | ENST00000368487; ENSP00000357472; ENSG00000143515 [P98198-4] ENST00000672630; ENSP00000500034; ENSG00000143515 [P98198-3] |
GeneIDi | 57198 |
KEGGi | hsa:57198 |
UCSCi | uc001few.4, human [P98198-1] |
Organism-specific databases
CTDi | 57198 |
DisGeNETi | 57198 |
GeneCardsi | ATP8B2 |
HGNCi | HGNC:13534, ATP8B2 |
HPAi | ENSG00000143515, Low tissue specificity |
MIMi | 605867, gene |
neXtProti | NX_P98198 |
OpenTargetsi | ENSG00000143515 |
PharmGKBi | PA25167 |
VEuPathDBi | HostDB:ENSG00000143515.16 |
HUGEi | Search... |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG0206, Eukaryota |
GeneTreei | ENSGT00940000160804 |
HOGENOMi | CLU_000846_8_4_1 |
InParanoidi | P98198 |
OMAi | RMENDQF |
PhylomeDBi | P98198 |
TreeFami | TF300654 |
Enzyme and pathway databases
PathwayCommonsi | P98198 |
Reactomei | R-HSA-936837, Ion transport by P-type ATPases |
Miscellaneous databases
BioGRID-ORCSi | 57198, 7 hits in 873 CRISPR screens |
ChiTaRSi | ATP8B2, human |
GenomeRNAii | 57198 |
Pharosi | P98198, Tdark |
PROi | PR:P98198 |
RNActi | P98198, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000143515, Expressed in thoracic aorta and 219 other tissues |
Genevisiblei | P98198, HS |
Family and domain databases
Gene3Di | 3.40.1110.10, 2 hits 3.40.50.1000, 2 hits |
InterProi | View protein in InterPro IPR030347, ATP8B2 IPR023299, ATPase_P-typ_cyto_dom_N IPR018303, ATPase_P-typ_P_site IPR023298, ATPase_P-typ_TM_dom_sf IPR008250, ATPase_P-typ_transduc_dom_A_sf IPR036412, HAD-like_sf IPR023214, HAD_sf IPR006539, P-type_ATPase_IV IPR032631, P-type_ATPase_N IPR001757, P_typ_ATPase IPR032630, P_typ_ATPase_c |
PANTHERi | PTHR24092, PTHR24092, 1 hit PTHR24092:SF46, PTHR24092:SF46, 1 hit |
Pfami | View protein in Pfam PF16212, PhoLip_ATPase_C, 1 hit PF16209, PhoLip_ATPase_N, 1 hit |
SUPFAMi | SSF56784, SSF56784, 1 hit SSF81653, SSF81653, 1 hit SSF81660, SSF81660, 1 hit SSF81665, SSF81665, 1 hit |
TIGRFAMsi | TIGR01652, ATPase-Plipid, 1 hit TIGR01494, ATPase_P-type, 1 hit |
PROSITEi | View protein in PROSITE PS00154, ATPASE_E1_E2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | AT8B2_HUMAN | |
Accessioni | P98198Primary (citable) accession number: P98198 Secondary accession number(s): B4E3P4 Q96NQ7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 30, 2000 |
Last sequence update: | April 30, 2003 | |
Last modified: | February 10, 2021 | |
This is version 182 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 1
Human chromosome 1: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families