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UniProtKB - P98198 (AT8B2_HUMAN)

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Protein

Phospholipid-transporting ATPase ID

Gene

ATP8B2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic component of a P4-ATPase flippase complex which catalyzes the hydrolysis of ATP coupled to the transport of aminophospholipids from the outer to the inner leaflet of various membranes and ensures the maintenance of asymmetric distribution of phospholipids. Phospholipid translocation seems also to be implicated in vesicle formation and in uptake of lipid signaling molecules (Probable).Curated

Catalytic activityi

ATP + H2O + phospholipid(Side 1) = ADP + phosphate + phospholipid(Side 2).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei4114-aspartylphosphate intermediateBy similarity1
Metal bindingi833MagnesiumBy similarity1
Metal bindingi837MagnesiumBy similarity1

GO - Molecular functioni

View the complete GO annotation on QuickGO ...

GO - Biological processi

View the complete GO annotation on QuickGO ...

Keywordsi

Molecular functionHydrolase
Biological processLipid transport, Transport
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-HSA-936837 Ion transport by P-type ATPases

Names & Taxonomyi

Protein namesi
Recommended name:
Phospholipid-transporting ATPase ID (EC:3.6.3.1)
Alternative name(s):
ATPase class I type 8B member 2
P4-ATPase flippase complex alpha subunit ATP8B2
Gene namesi
Name:ATP8B2
Synonyms:ATPID, KIAA1137
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

EuPathDBiHostDB:ENSG00000143515.16
HGNCiHGNC:13534 ATP8B2
MIMi605867 gene
neXtProtiNX_P98198

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 64CytoplasmicSequence analysisAdd BLAST64
Transmembranei65 – 86HelicalSequence analysisAdd BLAST22
Topological domaini87 – 92Exoplasmic loopSequence analysis6
Transmembranei93 – 112HelicalSequence analysisAdd BLAST20
Topological domaini113 – 295CytoplasmicSequence analysisAdd BLAST183
Transmembranei296 – 317HelicalSequence analysisAdd BLAST22
Topological domaini318 – 346Exoplasmic loopSequence analysisAdd BLAST29
Transmembranei347 – 368HelicalSequence analysisAdd BLAST22
Topological domaini369 – 889CytoplasmicSequence analysisAdd BLAST521
Transmembranei890 – 910HelicalSequence analysisAdd BLAST21
Topological domaini911 – 922Exoplasmic loopSequence analysisAdd BLAST12
Transmembranei923 – 942HelicalSequence analysisAdd BLAST20
Topological domaini943 – 972CytoplasmicSequence analysisAdd BLAST30
Transmembranei973 – 994HelicalSequence analysisAdd BLAST22
Topological domaini995 – 1008Exoplasmic loopSequence analysisAdd BLAST14
Transmembranei1009 – 1031HelicalSequence analysisAdd BLAST23
Topological domaini1032 – 1037CytoplasmicSequence analysis6
Transmembranei1038 – 1058HelicalSequence analysisAdd BLAST21
Topological domaini1059 – 1078Exoplasmic loopSequence analysisAdd BLAST20
Transmembranei1079 – 1103HelicalSequence analysisAdd BLAST25
Topological domaini1104 – 1209CytoplasmicSequence analysisAdd BLAST106

Keywords - Cellular componenti

Cell membrane, Endoplasmic reticulum, Membrane

Pathology & Biotechi

Organism-specific databases

OpenTargetsiENSG00000143515
PharmGKBiPA25167

Polymorphism and mutation databases

BioMutaiATP8B2
DMDMi30316371

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000463651 – 1209Phospholipid-transporting ATPase IDAdd BLAST1209

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1175PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiP98198
MaxQBiP98198
PaxDbiP98198
PeptideAtlasiP98198
PRIDEiP98198
ProteomicsDBi57827
57828 [P98198-2]
57829 [P98198-3]
57830 [P98198-4]

PTM databases

iPTMnetiP98198
PhosphoSitePlusiP98198

Expressioni

Tissue specificityi

Isoform 3 is ubiquitous, with highest expression in aorta, cerebellum and uterus.1 Publication

Gene expression databases

BgeeiENSG00000143515
CleanExiHS_ATP8B2
GenevisibleiP98198 HS

Organism-specific databases

HPAiHPA046680

Interactioni

Subunit structurei

Component of a P4-ATPase flippase complex which consists of a catalytic alpha subunit and an accessory beta subunit (Probable). The probable flippase ATP8B2:TMEM30A complex can form an intermediate phosphoenzyme in vitro. The interaction with TMEM30B is reported conflictingly.Curated

Protein-protein interaction databases

BioGridi121445, 18 interactors
IntActiP98198, 2 interactors
STRINGi9606.ENSP00000357475

Structurei

3D structure databases

ProteinModelPortaliP98198
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IV subfamily. [View classification]Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0206 Eukaryota
COG0474 LUCA
GeneTreeiENSGT00910000144008
HOGENOMiHOG000202528
HOVERGENiHBG050601
InParanoidiP98198
KOiK01530
OMAiGFGELIM
OrthoDBiEOG091G0139
PhylomeDBiP98198
TreeFamiTF300654

Family and domain databases

Gene3Di3.40.1110.10, 2 hits
3.40.50.1000, 2 hits
InterProiView protein in InterPro
IPR030347 ATP8B2
IPR023299 ATPase_P-typ_cyto_dom_N
IPR018303 ATPase_P-typ_P_site
IPR023298 ATPase_P-typ_TM_dom_sf
IPR008250 ATPase_P-typ_transduc_dom_A_sf
IPR036412 HAD-like_sf
IPR023214 HAD_sf
IPR006539 P-type_ATPase_IV
IPR032631 P-type_ATPase_N
IPR001757 P_typ_ATPase
IPR032630 P_typ_ATPase_c
PANTHERiPTHR24092 PTHR24092, 1 hit
PTHR24092:SF46 PTHR24092:SF46, 1 hit
PfamiView protein in Pfam
PF16212 PhoLip_ATPase_C, 1 hit
PF16209 PhoLip_ATPase_N, 1 hit
SUPFAMiSSF56784 SSF56784, 3 hits
SSF81653 SSF81653, 2 hits
SSF81660 SSF81660, 1 hit
SSF81665 SSF81665, 3 hits
TIGRFAMsiTIGR01652 ATPase-Plipid, 1 hit
TIGR01494 ATPase_P-type, 1 hit
PROSITEiView protein in PROSITE
PS00154 ATPASE_E1_E2, 1 hit

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P98198-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTVPKEMPEK WARAQAPPSW SRKKPSWGTE EERRARANDR EYNEKFQYAS 
        60         70         80         90        100
NCIKTSKYNI LTFLPVNLFE QFQEVANTYF LFLLILQLIP QISSLSWFTT 
       110        120        130        140        150
IVPLVLVLTI TAVKDATDDY FRHKSDNQVN NRQSQVLING ILQQEQWMNV 
       160        170        180        190        200
CVGDIIKLEN NQFVAADLLL LSSSEPHGLC YIETAELDGE TNMKVRQAIP 
       210        220        230        240        250
VTSELGDISK LAKFDGEVIC EPPNNKLDKF SGTLYWKENK FPLSNQNMLL 
       260        270        280        290        300
RGCVLRNTEW CFGLVIFAGP DTKLMQNSGR TKFKRTSIDR LMNTLVLWIF 
       310        320        330        340        350
GFLVCMGVIL AIGNAIWEHE VGMRFQVYLP WDEAVDSAFF SGFLSFWSYI 
       360        370        380        390        400
IILNTVVPIS LYVSVEVIRL GHSYFINWDK KMFCMKKRTP AEARTTTLNE 
       410        420        430        440        450
ELGQVEYIFS DKTGTLTQNI MVFNKCSING HSYGDVFDVL GHKAELGERP 
       460        470        480        490        500
EPVDFSFNPL ADKKFLFWDP SLLEAVKIGD PHTHEFFRLL SLCHTVMSEE 
       510        520        530        540        550
KNEGELYYKA QSPDEGALVT AARNFGFVFR SRTPKTITVH EMGTAITYQL 
       560        570        580        590        600
LAILDFNNIR KRMSVIVRNP EGKIRLYCKG ADTILLDRLH HSTQELLNTT 
       610        620        630        640        650
MDHLNEYAGE GLRTLVLAYK DLDEEYYEEW AERRLQASLA QDSREDRLAS 
       660        670        680        690        700
IYEEVENNMM LLGATAIEDK LQQGVPETIA LLTLANIKIW VLTGDKQETA 
       710        720        730        740        750
VNIGYSCKML TDDMTEVFIV TGHTVLEVRE ELRKAREKMM DSSRSVGNGF 
       760        770        780        790        800
TYQDKLSSSK LTSVLEAVAG EYALVINGHS LAHALEADME LEFLETACAC 
       810        820        830        840        850
KAVICCRVTP LQKAQVVELV KKYKKAVTLA IGDGANDVSM IKTAHIGVGI 
       860        870        880        890        900
SGQEGIQAVL ASDYSFSQFK FLQRLLLVHG RWSYLRMCKF LCYFFYKNFA 
       910        920        930        940        950
FTMVHFWFGF FCGFSAQTVY DQYFITLYNI VYTSLPVLAM GVFDQDVPEQ 
       960        970        980        990       1000
RSMEYPKLYE PGQLNLLFNK REFFICIAQG IYTSVLMFFI PYGVFADATR 
      1010       1020       1030       1040       1050
DDGTQLADYQ SFAVTVATSL VIVVSVQIGL DTGYWTAINH FFIWGSLAVY 
      1060       1070       1080       1090       1100
FAILFAMHSN GLFDMFPNQF RFVGNAQNTL AQPTVWLTIV LTTVVCIMPV 
      1110       1120       1130       1140       1150
VAFRFLRLNL KPDLSDTVRY TQLVRKKQKA QHRCMRRVGR TGSRRSGYAF 
      1160       1170       1180       1190       1200
SHQEGFGELI MSGKNMRLSS LALSSFTTRS SSSWIESLRR KKSDSASSPS 

GGADKPLKG
Note: No experimental confirmation available.
Length:1,209
Mass (Da):137,440
Last modified:April 30, 2003 - v2
Checksum:iD905A97E9D9724BD
GO
Isoform 2 (identifier: P98198-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     434-461: GDVFDVLGHKAELGERPEPVDFSFNPLA → VGSEGPRRKGPSWPGTVAHAYSHSTLGG
     462-1209: Missing.

Show »
Length:461
Mass (Da):52,624
Checksum:i15CAABEAAE6862DC
GO
Isoform 3 (identifier: P98198-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-29: MTVPKEMPEKWARAQAPPSWSRKKPSWGT → MDTLRAVPLFSISGLFSFPYRVSHGIAGILLGEMAVCAKKRPP

Show »
Length:1,223
Mass (Da):138,719
Checksum:i61AF805A0511A143
GO
Isoform 4 (identifier: P98198-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-29: MTVPKEMPEKWARAQAPPSWSRKKPSWGT → MAVCAKKRPP
     364-406: SVEVIRLGHS...TLNEELGQVE → RYVPSLTWGL...KSSSSCTVNI
     407-1209: Missing.

Show »
Length:387
Mass (Da):44,213
Checksum:iB4C17CA615F4F952
GO

Sequence cautioni

The sequence AAH30288 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAB70822 differs from that shown. Reason: Frameshift at position 364.Curated
The sequence BAG65556 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti834G → E in BAA86451 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0371471 – 29MTVPK…PSWGT → MDTLRAVPLFSISGLFSFPY RVSHGIAGILLGEMAVCAKK RPP in isoform 3. 1 PublicationAdd BLAST29
Alternative sequenceiVSP_0371481 – 29MTVPK…PSWGT → MAVCAKKRPP in isoform 4. 1 PublicationAdd BLAST29
Alternative sequenceiVSP_037149364 – 406SVEVI…LGQVE → RYVPSLTWGLSRESGGPIEL FFSMKMKSLRSNEKSSSSCT VNI in isoform 4. 1 PublicationAdd BLAST43
Alternative sequenceiVSP_037150407 – 1209Missing in isoform 4. 1 PublicationAdd BLAST803
Alternative sequenceiVSP_007302434 – 461GDVFD…FNPLA → VGSEGPRRKGPSWPGTVAHA YSHSTLGG in isoform 2. 1 PublicationAdd BLAST28
Alternative sequenceiVSP_007303462 – 1209Missing in isoform 2. 1 PublicationAdd BLAST748

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY302537 mRNA Translation: AAQ19027.1
AK054886 mRNA Translation: BAB70822.1 Frameshift.
AK304806 mRNA Translation: BAG65556.1 Different initiation.
AL162591 Genomic DNA No translation available.
CH471121 Genomic DNA Translation: EAW53205.1
BC007837 mRNA Translation: AAH07837.2
BC030288 mRNA Translation: AAH30288.1 Different initiation.
BC069264 mRNA Translation: AAH69264.1
AB032963 mRNA Translation: BAA86451.1
AL137537 mRNA Translation: CAB70799.1
CCDSiCCDS1066.1 [P98198-3]
CCDS41405.1 [P98198-4]
PIRiT46381
RefSeqiNP_001005855.1, NM_001005855.1 [P98198-4]
NP_065185.1, NM_020452.3 [P98198-3]
XP_005245412.1, XM_005245355.2 [P98198-1]
UniGeneiHs.435700

Genome annotation databases

EnsembliENST00000368487; ENSP00000357472; ENSG00000143515 [P98198-4]
ENST00000368489; ENSP00000357475; ENSG00000143515 [P98198-3]
GeneIDi57198
KEGGihsa:57198
UCSCiuc001few.4 human [P98198-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiAT8B2_HUMAN
AccessioniPrimary (citable) accession number: P98198
Secondary accession number(s): B4E3P4
, Q6NT69, Q7Z486, Q96I43, Q96NQ7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: April 30, 2003
Last modified: June 20, 2018
This is version 163 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

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