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UniProtKB - P98195 (ATP9B_MOUSE)
Protein
Probable phospholipid-transporting ATPase IIB
Gene
Atp9b
Organism
Mus musculus (Mouse)
Status
Functioni
Catalytic activityi
- ATP + H(2)O + phospholipid(Side 1) = ADP + phosphate + phospholipid(Side 2). EC:7.6.2.1
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 467 | 4-aspartylphosphate intermediateBy similarity | 1 | |
Metal bindingi | 873 | MagnesiumBy similarity | 1 | |
Metal bindingi | 877 | MagnesiumBy similarity | 1 |
GO - Molecular functioni
- ATPase-coupled intramembrane lipid transporter activity Source: GO_Central
- ATP binding Source: UniProtKB-KW
- magnesium ion binding Source: InterPro
GO - Biological processi
- endocytosis Source: GO_Central
- phospholipid translocation Source: GO_Central
- retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum Source: GO_Central
Keywordsi
Molecular function | Translocase |
Ligand | ATP-binding, Magnesium, Metal-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-MMU-936837, Ion transport by P-type ATPases |
Names & Taxonomyi
Protein namesi | Recommended name: Probable phospholipid-transporting ATPase IIB (EC:7.6.2.1)Alternative name(s): ATPase class II type 9B |
Gene namesi | Name:Atp9b |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1354757, Atp9b |
Subcellular locationi
Golgi apparatus
- trans-Golgi network membrane By similarity; Multi-pass membrane protein By similarity
Endosome
- endosome Source: GO_Central
Golgi apparatus
- trans-Golgi network Source: MGI
Plasma Membrane
- plasma membrane Source: GO_Central
Other locations
- integral component of membrane Source: UniProtKB-KW
- perinuclear region of cytoplasm Source: MGI
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 143 | CytoplasmicSequence analysisAdd BLAST | 143 | |
Transmembranei | 144 – 164 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 165 – 172 | ExtracellularSequence analysis | 8 | |
Transmembranei | 173 – 193 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 194 – 381 | CytoplasmicSequence analysisAdd BLAST | 188 | |
Transmembranei | 382 – 402 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 403 – 407 | ExtracellularSequence analysis | 5 | |
Transmembranei | 408 – 427 | HelicalSequence analysisAdd BLAST | 20 | |
Topological domaini | 428 – 938 | CytoplasmicSequence analysisAdd BLAST | 511 | |
Transmembranei | 939 – 959 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 960 – 961 | ExtracellularSequence analysis | 2 | |
Transmembranei | 962 – 982 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 983 – 1011 | CytoplasmicSequence analysisAdd BLAST | 29 | |
Transmembranei | 1012 – 1032 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 1033 – 1040 | ExtracellularSequence analysis | 8 | |
Transmembranei | 1041 – 1061 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 1062 – 1065 | CytoplasmicSequence analysis | 4 | |
Transmembranei | 1066 – 1086 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 1087 – 1105 | ExtracellularSequence analysisAdd BLAST | 19 | |
Transmembranei | 1106 – 1128 | HelicalSequence analysisAdd BLAST | 23 | |
Topological domaini | 1129 – 1146 | CytoplasmicSequence analysisAdd BLAST | 18 |
Keywords - Cellular componenti
Golgi apparatus, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000046378 | 1 – 1146 | Probable phospholipid-transporting ATPase IIBAdd BLAST | 1146 |
Proteomic databases
MaxQBi | P98195 |
PaxDbi | P98195 |
PRIDEi | P98195 |
ProteomicsDBi | 277133 [P98195-1] 277134 [P98195-2] |
PTM databases
iPTMneti | P98195 |
PhosphoSitePlusi | P98195 |
SwissPalmi | P98195 |
Expressioni
Tissue specificityi
Found in most tissues except spleen and muscle. Most abundant in testis. Also detected in fetal tissues.
Interactioni
Protein-protein interaction databases
BioGRIDi | 206103, 1 interactor |
STRINGi | 10090.ENSMUSP00000089394 |
Miscellaneous databases
RNActi | P98195, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 508 – 535 | DisorderedSequence analysisAdd BLAST | 28 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 508 – 534 | Polar residuesSequence analysisAdd BLAST | 27 |
Sequence similaritiesi
Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IV subfamily. [View classification]Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG0210, Eukaryota |
InParanoidi | P98195 |
OrthoDBi | 587717at2759 |
PhylomeDBi | P98195 |
Family and domain databases
Gene3Di | 3.40.1110.10, 1 hit 3.40.50.1000, 1 hit |
InterProi | View protein in InterPro IPR030355, ATP9B IPR023299, ATPase_P-typ_cyto_dom_N IPR018303, ATPase_P-typ_P_site IPR023298, ATPase_P-typ_TM_dom_sf IPR008250, ATPase_P-typ_transduc_dom_A_sf IPR036412, HAD-like_sf IPR023214, HAD_sf IPR006539, P-type_ATPase_IV IPR032631, P-type_ATPase_N IPR001757, P_typ_ATPase IPR032630, P_typ_ATPase_c IPR044492, P_typ_ATPase_HD_dom |
PANTHERi | PTHR24092:SF50, PTHR24092:SF50, 1 hit |
Pfami | View protein in Pfam PF16212, PhoLip_ATPase_C, 1 hit PF16209, PhoLip_ATPase_N, 1 hit |
SFLDi | SFLDF00027, p-type_atpase, 1 hit |
SUPFAMi | SSF56784, SSF56784, 1 hit SSF81653, SSF81653, 1 hit SSF81660, SSF81660, 1 hit SSF81665, SSF81665, 1 hit |
TIGRFAMsi | TIGR01652, ATPase-Plipid, 1 hit TIGR01494, ATPase_P-type, 3 hits |
PROSITEi | View protein in PROSITE PS00154, ATPASE_E1_E2, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 6 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: P98195-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MADQIPLYPV RSAGAAASHR RAAYYSSAGP GPGADRRGRY QLEDESAHLD
60 70 80 90 100
EMPLMMSEEG FENDESDYHT LPRARITRRK RGLEWFVCGG WKFLCTSCCD
110 120 130 140 150
WLINVCQRKK ELKARTVWLG CPEKCEEKHP RNSIKNQKYN VFTFIPGVLY
160 170 180 190 200
EQFKFFLNLY FLVVSCSQFV PALKIGYLYT YWAPLGFVLA VTIAREAIDE
210 220 230 240 250
FRRFQRDKEM NSQLYSKLTV RGKVQVKSSD IQVGDLIIVE KNQRIPSDMV
260 270 280 290 300
FLRTSEKAGS CFIRTDQLDG ETDWKLKVAV SCTQRLPALG DLFSISAYVY
310 320 330 340 350
AQKPQLDIHS FEGTFTREDS DPPIHESLSI ENTLWASTIV ASGTVIGVVI
360 370 380 390 400
YTGKETRSVM NTSNPNNKVG LLDLELNQLT KALFLALVVL SVVMVTLQGF
410 420 430 440 450
AGPWYRNLFR FLLLFSYIIP ISLRVNLDMG KAAYGWMIMK DENIPGTVVR
460 470 480 490 500
TSTIPEELGR LVYLLTDKTG TLTQNEMVFK RLHLGTVSYG TDTMDEIQSH
510 520 530 540 550
VLNSYLQVHS QPSGHNPSSA PLRRSQSSTP KVKKSVSSRI HEAVKAIALC
560 570 580 590 600
HNVTPVYEAR AGITGETEFA EADQDFSDEN RTYQASSPDE VALVRWTESV
610 620 630 640 650
GLTLVSRDLA SMQLKTPSGQ VLTYCILQMF PFTSESKRMG IIVRDESTAE
660 670 680 690 700
ITFYMKGADV AMSTIVQYND WLEEECGNMA REGLRTLVVA KRTLTEEQYQ
710 720 730 740 750
DFESRYSQAK LSIHDRALKV AAVVESLERE MELLCLTGVE DQLQADVRPT
760 770 780 790 800
LEMLRNAGIK IWMLTGDKLE TATCIAKSSH LVSRTQDIHV FRPVTSRGEA
810 820 830 840 850
HLELNAFRRK HDCALVISGD SLEVCLRYYE HELVELACQC PAVVCCRCSP
860 870 880 890 900
TQKAHIVTLL RQHTRKRTCA IGDGGNDVSM IQAADCGIGI EGKEGKQASL
910 920 930 940 950
AADFSITQFR HIGRLLMVHG RNSYKRSAAL GQFVMHRGLI ISTMQAVFSS
960 970 980 990 1000
VFYFASVPLY QGFLMVGYAT IYTMFPVFSL VLDQDVKPEM AILYPELYKD
1010 1020 1030 1040 1050
LTKGRSLSFK TFLIWVLISI YQGGILMYGA LLLFEDEFVH VVAISFTALI
1060 1070 1080 1090 1100
LTELLMVALT IRTWHWLMVV AEFLSLGCYV ASLAFLNEYF GIGRVSFGAF
1110 1120 1130 1140
LDVAFITTVT FLWKVSAITV VSCLPLYVLK YLKRKLSPPS YSKLSS
Computationally mapped potential isoform sequencesi
There are 6 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketD3YV00 | D3YV00_MOUSE | Phospholipid-transporting ATPase | Atp9b | 1,146 | Annotation score: | ||
A0A286YCV0 | A0A286YCV0_MOUSE | Phospholipid-transporting ATPase | Atp9b | 1,135 | Annotation score: | ||
A0A286YDR0 | A0A286YDR0_MOUSE | Probable phospholipid-transporting ... | Atp9b | 235 | Annotation score: | ||
A0A286YCT2 | A0A286YCT2_MOUSE | Probable phospholipid-transporting ... | Atp9b | 380 | Annotation score: | ||
A0A286YDF8 | A0A286YDF8_MOUSE | Probable phospholipid-transporting ... | Atp9b | 78 | Annotation score: | ||
A0A286YDZ3 | A0A286YDZ3_MOUSE | Probable phospholipid-transporting ... | Atp9b | 163 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 366 | N → K in AAH79626 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 605 | V → I in AAH79626 (PubMed:15489334).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_035908 | 1091 – 1101 | Missing in isoform 2. 1 PublicationAdd BLAST | 11 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC003246 mRNA Translation: AAH03246.1 BC079626 mRNA Translation: AAH79626.1 AF155913 mRNA Translation: AAF08476.1 |
CCDSi | CCDS29370.1 [P98195-2] CCDS89288.1 [P98195-1] |
RefSeqi | NP_001188498.1, NM_001201569.1 NP_056620.2, NM_015805.3 |
Genome annotation databases
GeneIDi | 50771 |
KEGGi | mmu:50771 |
UCSCi | uc008fti.2, mouse [P98195-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC003246 mRNA Translation: AAH03246.1 BC079626 mRNA Translation: AAH79626.1 AF155913 mRNA Translation: AAF08476.1 |
CCDSi | CCDS29370.1 [P98195-2] CCDS89288.1 [P98195-1] |
RefSeqi | NP_001188498.1, NM_001201569.1 NP_056620.2, NM_015805.3 |
3D structure databases
SMRi | P98195 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 206103, 1 interactor |
STRINGi | 10090.ENSMUSP00000089394 |
PTM databases
iPTMneti | P98195 |
PhosphoSitePlusi | P98195 |
SwissPalmi | P98195 |
Proteomic databases
MaxQBi | P98195 |
PaxDbi | P98195 |
PRIDEi | P98195 |
ProteomicsDBi | 277133 [P98195-1] 277134 [P98195-2] |
Protocols and materials databases
DNASUi | 50771 |
Genome annotation databases
GeneIDi | 50771 |
KEGGi | mmu:50771 |
UCSCi | uc008fti.2, mouse [P98195-1] |
Organism-specific databases
CTDi | 374868 |
MGIi | MGI:1354757, Atp9b |
Phylogenomic databases
eggNOGi | KOG0210, Eukaryota |
InParanoidi | P98195 |
OrthoDBi | 587717at2759 |
PhylomeDBi | P98195 |
Enzyme and pathway databases
Reactomei | R-MMU-936837, Ion transport by P-type ATPases |
Miscellaneous databases
BioGRID-ORCSi | 50771, 3 hits in 64 CRISPR screens |
ChiTaRSi | Atp9b, mouse |
PROi | PR:P98195 |
RNActi | P98195, protein |
SOURCEi | Search... |
Family and domain databases
Gene3Di | 3.40.1110.10, 1 hit 3.40.50.1000, 1 hit |
InterProi | View protein in InterPro IPR030355, ATP9B IPR023299, ATPase_P-typ_cyto_dom_N IPR018303, ATPase_P-typ_P_site IPR023298, ATPase_P-typ_TM_dom_sf IPR008250, ATPase_P-typ_transduc_dom_A_sf IPR036412, HAD-like_sf IPR023214, HAD_sf IPR006539, P-type_ATPase_IV IPR032631, P-type_ATPase_N IPR001757, P_typ_ATPase IPR032630, P_typ_ATPase_c IPR044492, P_typ_ATPase_HD_dom |
PANTHERi | PTHR24092:SF50, PTHR24092:SF50, 1 hit |
Pfami | View protein in Pfam PF16212, PhoLip_ATPase_C, 1 hit PF16209, PhoLip_ATPase_N, 1 hit |
SFLDi | SFLDF00027, p-type_atpase, 1 hit |
SUPFAMi | SSF56784, SSF56784, 1 hit SSF81653, SSF81653, 1 hit SSF81660, SSF81660, 1 hit SSF81665, SSF81665, 1 hit |
TIGRFAMsi | TIGR01652, ATPase-Plipid, 1 hit TIGR01494, ATPase_P-type, 3 hits |
PROSITEi | View protein in PROSITE PS00154, ATPASE_E1_E2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ATP9B_MOUSE | |
Accessioni | P98195Primary (citable) accession number: P98195 Secondary accession number(s): Q68FM3, Q99LI3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 30, 2000 |
Last sequence update: | December 16, 2008 | |
Last modified: | February 23, 2022 | |
This is version 163 of the entry and version 4 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families