UniProtKB - P98192 (GNPAT_MOUSE)
Protein
Dihydroxyacetone phosphate acyltransferase
Gene
Gnpat
Organism
Mus musculus (Mouse)
Status
Functioni
Dihydroxyacetonephosphate acyltransferase involved in plasmalogen biosynthesis.By similarity
Catalytic activityi
- EC:2.3.1.42
- dihydroxyacetone phosphate + hexadecanoyl-CoA = 1-hexadecanoylglycerone 3-phosphate + CoABy similarityThis reaction proceeds in the forwardBy similarity direction.
: glycerophospholipid metabolism Pathwayi
This protein is involved in the pathway glycerophospholipid metabolism, which is part of Membrane lipid metabolism.View all proteins of this organism that are known to be involved in the pathway glycerophospholipid metabolism and in Membrane lipid metabolism.
GO - Molecular functioni
- catalytic activity Source: MGI
- glycerone-phosphate O-acyltransferase activity Source: MGI
- palmitoyl-CoA hydrolase activity Source: MGI
GO - Biological processi
- cerebellum morphogenesis Source: MGI
- ether lipid biosynthetic process Source: MGI
- fatty acid metabolic process Source: GO_Central
- glycerophospholipid metabolic process Source: UniProtKB-UniPathway
- membrane organization Source: MGI
- myelination Source: MGI
- paranodal junction assembly Source: MGI
- phospholipid biosynthetic process Source: GO_Central
- response to drug Source: Ensembl
- response to fatty acid Source: Ensembl
- response to nutrient Source: Ensembl
- response to starvation Source: Ensembl
- synapse assembly Source: MGI
Keywordsi
Molecular function | Acyltransferase, Transferase |
Enzyme and pathway databases
Reactomei | R-MMU-1483166, Synthesis of PA R-MMU-75896, Plasmalogen biosynthesis R-MMU-9033241, Peroxisomal protein import |
UniPathwayi | UPA00940 |
Names & Taxonomyi
Protein namesi | Recommended name: Dihydroxyacetone phosphate acyltransferaseCurated (EC:2.3.1.42)Short name: DAP-AT Short name: DHAP-AT Alternative name(s): Acyl-CoA:dihydroxyacetonephosphateacyltransferase Glycerone-phosphate O-acyltransferase |
Gene namesi | |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1343460, Gnpat |
Subcellular locationi
Peroxisome
- Peroxisome membrane By similarity; Peripheral membrane protein By similarity; Matrix side By similarity
Note: Exclusively localized to the lumenal side of the peroxisomal membrane.By similarity
Mitochondrion
- mitochondrial membrane Source: GO_Central
- mitochondrion Source: MGI
Peroxisome
- peroxisomal matrix Source: MGI
- peroxisomal membrane Source: MGI
- peroxisome Source: MGI
Keywords - Cellular componenti
Membrane, PeroxisomePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000195247 | 1 – 678 | Dihydroxyacetone phosphate acyltransferaseAdd BLAST | 678 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 12 | PhosphoserineCombined sources | 1 | |
Modified residuei | 17 | PhosphoserineBy similarity | 1 | |
Modified residuei | 641 | N6-acetyllysineBy similarity | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
EPDi | P98192 |
jPOSTi | P98192 |
PaxDbi | P98192 |
PeptideAtlasi | P98192 |
PRIDEi | P98192 |
PTM databases
iPTMneti | P98192 |
PhosphoSitePlusi | P98192 |
Expressioni
Tissue specificityi
Highly expressed in liver and testis. Lower levels in heart, brain, lung and kidney. Detected in spleen.
Gene expression databases
Bgeei | ENSMUSG00000031985, Expressed in embryonic post-anal tail and 328 other tissues |
ExpressionAtlasi | P98192, baseline and differential |
Genevisiblei | P98192, MM |
Interactioni
Subunit structurei
May be part of a heterotrimeric complex composed of DAP-AT, ADAP-S and a modified form of DAP-AT.
Protein-protein interaction databases
BioGRIDi | 199996, 1 interactor |
IntActi | P98192, 2 interactors |
MINTi | P98192 |
STRINGi | 10090.ENSMUSP00000034466 |
Miscellaneous databases
RNActi | P98192, protein |
Family & Domainsi
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 161 – 166 | HXXXXD motif | 6 | |
Motifi | 676 – 678 | Microbody targeting signalSequence analysis | 3 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 5 – 9 | Poly-Ser | 5 |
Domaini
The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate.By similarity
Sequence similaritiesi
Belongs to the GPAT/DAPAT family.Curated
Phylogenomic databases
eggNOGi | KOG3730, Eukaryota |
GeneTreei | ENSGT00520000055570 |
InParanoidi | P98192 |
OMAi | MLKNGFA |
OrthoDBi | 198209at2759 |
PhylomeDBi | P98192 |
TreeFami | TF313360 |
Family and domain databases
CDDi | cd07993, LPLAT_DHAPAT-like, 1 hit |
InterProi | View protein in InterPro IPR028353, DHAPAT IPR022284, GPAT/DHAPAT IPR041728, GPAT/DHAPAT_LPLAT IPR002123, Plipid/glycerol_acylTrfase |
PANTHERi | PTHR12563, PTHR12563, 1 hit |
Pfami | View protein in Pfam PF01553, Acyltransferase, 1 hit |
PIRSFi | PIRSF500063, DHAPAT, 1 hit PIRSF000437, GPAT_DHAPAT, 1 hit |
SMARTi | View protein in SMART SM00563, PlsC, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
P98192-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MDVPSSSSSR FSVGSASPSS VLLYAKDLKK WDEFEDLLEE RRHISDFKFA
60 70 80 90 100
MKCYTPPLYR GITPCKPGDI KSIVLSSEEI NYVIKQLSRE SLTGVDVLRE
110 120 130 140 150
EASEILEEMS HKLRIGAIRF FAFVLSKIFK QIFSKVCVNE EGIQKLQRAV
160 170 180 190 200
QEHPVVLLPS HRSYIDFLML SFILYSYDLP VPVIAAGMDF LGMRVVSELL
210 220 230 240 250
RMSGAFFMRR TFGGNKLYWA VFSEYVKTML RCGYAPVEFF LEGTRSRAAK
260 270 280 290 300
TLTPKFGLLN IVMEPFFKRE VFDTYFVPIS ISYDKILEES LYAYEILGVP
310 320 330 340 350
KPKESTTGLL KARRILSENF GSIHVYFGDP VSLRSLAAGR LNRNTYNLVP
360 370 380 390 400
RCIPQKQPED VQAFVTEVAY KMQLLQIENL ALSPWLLVVT ILLQNQLSMD
410 420 430 440 450
FDALVEKTLW LKGVTQVFGG FLLWPDNKLP EEVVQSSILL HSNLASLVKD
460 470 480 490 500
QVVLKMNSGS SQVVNGLVPE HIALLMCSAY RNQLLNIFAR PSLVALALHM
510 520 530 540 550
TPGLRKEDVF SCFSFLRNVF SDEFIFLPGN TLRDFEEGCY LLCKAEAMQM
560 570 580 590 600
AGKDIILTDK GTAVLQFLTS LFKPFVESYQ LLCRYLLHEE DYFGEKEYLV
610 620 630 640 650
AARKFTRQLL DQGSSQCYDA LSSELQKNAL AAFVRLGVVE KKKVDSKYVY
660 670
YVNGPATSKL EEMLGCKKPI GKPATAKL
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketQ05DV2 | Q05DV2_MOUSE | Dihydroxyacetone phosphate acyltran... | Gnpat | 585 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF110769 mRNA Translation: AAD55351.1 AJ132012 mRNA Translation: CAB41975.1 |
CCDSi | CCDS22776.1 |
RefSeqi | NP_034452.3, NM_010322.3 |
Genome annotation databases
Ensembli | ENSMUST00000034466; ENSMUSP00000034466; ENSMUSG00000031985 |
GeneIDi | 14712 |
KEGGi | mmu:14712 |
UCSCi | uc009nxv.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF110769 mRNA Translation: AAD55351.1 AJ132012 mRNA Translation: CAB41975.1 |
CCDSi | CCDS22776.1 |
RefSeqi | NP_034452.3, NM_010322.3 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 199996, 1 interactor |
IntActi | P98192, 2 interactors |
MINTi | P98192 |
STRINGi | 10090.ENSMUSP00000034466 |
PTM databases
iPTMneti | P98192 |
PhosphoSitePlusi | P98192 |
Proteomic databases
EPDi | P98192 |
jPOSTi | P98192 |
PaxDbi | P98192 |
PeptideAtlasi | P98192 |
PRIDEi | P98192 |
Protocols and materials databases
Antibodypediai | 34685, 253 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000034466; ENSMUSP00000034466; ENSMUSG00000031985 |
GeneIDi | 14712 |
KEGGi | mmu:14712 |
UCSCi | uc009nxv.2, mouse |
Organism-specific databases
CTDi | 8443 |
MGIi | MGI:1343460, Gnpat |
Phylogenomic databases
eggNOGi | KOG3730, Eukaryota |
GeneTreei | ENSGT00520000055570 |
InParanoidi | P98192 |
OMAi | MLKNGFA |
OrthoDBi | 198209at2759 |
PhylomeDBi | P98192 |
TreeFami | TF313360 |
Enzyme and pathway databases
UniPathwayi | UPA00940 |
Reactomei | R-MMU-1483166, Synthesis of PA R-MMU-75896, Plasmalogen biosynthesis R-MMU-9033241, Peroxisomal protein import |
Miscellaneous databases
BioGRID-ORCSi | 14712, 1 hit in 17 CRISPR screens |
ChiTaRSi | Gnpat, mouse |
PROi | PR:P98192 |
RNActi | P98192, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000031985, Expressed in embryonic post-anal tail and 328 other tissues |
ExpressionAtlasi | P98192, baseline and differential |
Genevisiblei | P98192, MM |
Family and domain databases
CDDi | cd07993, LPLAT_DHAPAT-like, 1 hit |
InterProi | View protein in InterPro IPR028353, DHAPAT IPR022284, GPAT/DHAPAT IPR041728, GPAT/DHAPAT_LPLAT IPR002123, Plipid/glycerol_acylTrfase |
PANTHERi | PTHR12563, PTHR12563, 1 hit |
Pfami | View protein in Pfam PF01553, Acyltransferase, 1 hit |
PIRSFi | PIRSF500063, DHAPAT, 1 hit PIRSF000437, GPAT_DHAPAT, 1 hit |
SMARTi | View protein in SMART SM00563, PlsC, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | GNPAT_MOUSE | |
Accessioni | P98192Primary (citable) accession number: P98192 Secondary accession number(s): Q9WUT6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 30, 2000 |
Last sequence update: | May 30, 2000 | |
Last modified: | February 10, 2021 | |
This is version 154 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families