UniProtKB - P97879 (GRIP1_RAT)
Glutamate receptor-interacting protein 1
Grip1
Functioni
May play a role as a localized scaffold for the assembly of a multiprotein signaling complex and as mediator of the trafficking of its binding partners at specific subcellular location in neurons (PubMed:9069286).
Through complex formation with NSG1, GRIA2 and STX12 controls the intracellular fate of AMPAR and the endosomal sorting of the GRIA2 subunit toward recycling and membrane targeting (PubMed:16037816).
2 PublicationsGO - Molecular functioni
- glucocorticoid receptor binding Source: RGD
- protein C-terminus binding Source: RGD
- signaling receptor binding Source: RGD
- signaling receptor complex adaptor activity Source: InterPro
GO - Biological processi
- dendrite arborization Source: RGD
- nervous system development Source: RGD
- neurotransmitter receptor transport, endosome to postsynaptic membrane Source: RGD
- positive regulation of neuron projection arborization Source: ARUK-UCL
- protein localization Source: RGD
- protein transport Source: RGD
- vesicle-mediated transport in synapse Source: RGD
Enzyme and pathway databases
Reactomei | R-RNO-416993, Trafficking of GluR2-containing AMPA receptors |
Names & Taxonomyi
Protein namesi | Recommended name: Glutamate receptor-interacting protein 1Short name: GRIP-1 Alternative name(s): AMPA receptor-interacting protein GRIP1 |
Gene namesi | Name:Grip1 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 621667, Grip1 |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum membrane 1 Publication; Peripheral membrane protein Curated
Plasma membrane
- postsynaptic cell membrane 1 Publication
Cytoplasm and Cytosol
- Cytoplasm 1 Publication
Other locations
- Cytoplasmic vesicle 1 Publication
- Perikaryon 1 Publication
- dendrite 1 Publication
- Endomembrane system ; Peripheral membrane protein
- postsynaptic density 2 Publications
Note: Membrane-associated with vesicles, peri-Golgi complexes and endoplasmic reticulum. Enriched in postsynaptic plasma membrane and postsynaptic densities.1 Publication
Endoplasmic reticulum
- endoplasmic reticulum membrane Source: UniProtKB-SubCell
Plasma Membrane
- plasma membrane Source: RGD
- postsynaptic membrane Source: UniProtKB-SubCell
Other locations
- cytoplasmic vesicle Source: UniProtKB-KW
- dendrite Source: UniProtKB-SubCell
- glutamatergic synapse Source: SynGO
- membrane raft Source: RGD
- neuron projection Source: RGD
- neuronal cell body Source: UniProtKB
- perikaryon Source: UniProtKB-SubCell
- postsynapse Source: SynGO
- postsynaptic density Source: SynGO
Keywords - Cellular componenti
Cell junction, Cell membrane, Cell projection, Cytoplasm, Cytoplasmic vesicle, Endoplasmic reticulum, Membrane, Postsynaptic cell membrane, SynapsePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000083851 | 1 – 1112 | Glutamate receptor-interacting protein 1Add BLAST | 1112 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 43 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | P97879 |
PTM databases
iPTMneti | P97879 |
PhosphoSitePlusi | P97879 |
SwissPalmi | P97879 |
Expressioni
Tissue specificityi
Developmental stagei
Interactioni
Subunit structurei
Interacts with EFNB1, EPHA7, EPHB2, EFNB3, KIF5A, KIF5C, KIF5B and the C-terminal tail of PRLHR.
Forms a ternary complex with GRIA2 and CSPG4 (By similarity). Can form homomultimers or heteromultimers with GRIP2.
Interacts with GRIA2, GRIA3, GRIPAP1/GRASP1, PPFIA1, PPFIA4, FRAS1, PLCD4, PTPRF and liprins-alpha.
Interacts with ATAD1 in an ATP-dependent manner. ATAD1-catalyzed ATP hydrolysis disrupts binding to ATAD1 and to GRIA2 and leads to AMPAR complex disassembly.
Interacts with SLC30A9 (By similarity).
Interacts with BUD23 (By similarity).
Forms a complex with NSG1, GRIA2 and STX12; controls the intracellular fate of AMPAR and the endosomal sorting of the GRIA2 subunit toward recycling and membrane targeting (PubMed:16037816).
Interacts with NSG1 (PubMed:16037816).
By similarity1 PublicationBinary interactionsi
P97879
GO - Molecular functioni
- glucocorticoid receptor binding Source: RGD
- protein C-terminus binding Source: RGD
- signaling receptor binding Source: RGD
Protein-protein interaction databases
BioGRIDi | 249882, 11 interactors |
CORUMi | P97879 |
DIPi | DIP-37486N |
ELMi | P97879 |
IntActi | P97879, 34 interactors |
MINTi | P97879 |
STRINGi | 10116.ENSRNOP00000005539 |
Chemistry databases
BindingDBi | P97879 |
Structurei
Secondary structure
3D structure databases
SMRi | P97879 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P97879 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 53 – 136 | PDZ 1PROSITE-ProRule annotationAdd BLAST | 84 | |
Domaini | 150 – 238 | PDZ 2PROSITE-ProRule annotationAdd BLAST | 89 | |
Domaini | 252 – 336 | PDZ 3PROSITE-ProRule annotationAdd BLAST | 85 | |
Domaini | 471 – 560 | PDZ 4PROSITE-ProRule annotationAdd BLAST | 90 | |
Domaini | 572 – 657 | PDZ 5PROSITE-ProRule annotationAdd BLAST | 86 | |
Domaini | 672 – 754 | PDZ 6PROSITE-ProRule annotationAdd BLAST | 83 | |
Domaini | 988 – 1070 | PDZ 7PROSITE-ProRule annotationAdd BLAST | 83 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 752 – 796 | DisorderedSequence analysisAdd BLAST | 45 | |
Regioni | 841 – 886 | DisorderedSequence analysisAdd BLAST | 46 | |
Regioni | 922 – 963 | DisorderedSequence analysisAdd BLAST | 42 | |
Regioni | 1077 – 1112 | DisorderedSequence analysisAdd BLAST | 36 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 752 – 767 | Polar residuesSequence analysisAdd BLAST | 16 | |
Compositional biasi | 848 – 876 | Polar residuesSequence analysisAdd BLAST | 29 | |
Compositional biasi | 922 – 962 | Polar residuesSequence analysisAdd BLAST | 41 |
Domaini
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG3528, Eukaryota |
InParanoidi | P97879 |
OrthoDBi | 65191at2759 |
PhylomeDBi | P97879 |
Family and domain databases
Gene3Di | 2.30.42.10, 7 hits |
InterProi | View protein in InterPro IPR030026, GRIP1 IPR043545, GRIP1/2 IPR001478, PDZ IPR041489, PDZ_6 IPR036034, PDZ_sf |
PANTHERi | PTHR46227, PTHR46227, 1 hit PTHR46227:SF3, PTHR46227:SF3, 1 hit |
Pfami | View protein in Pfam PF00595, PDZ, 6 hits PF17820, PDZ_6, 1 hit |
SMARTi | View protein in SMART SM00228, PDZ, 7 hits |
SUPFAMi | SSF50156, SSF50156, 7 hits |
PROSITEi | View protein in PROSITE PS50106, PDZ, 7 hits |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 4 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MIAVSFKCRC QILRRLTKDE SPYTKSASQT KPPDGALAVR RQSIPEEFKG
60 70 80 90 100
STVVELMKKE GTTLGLTVSG GIDKDGKPRV SNLRQGGIAA RSDQLDVGDY
110 120 130 140 150
IKAVNGINLA KFRHDEIISL LKNVGERVVL EVEYELPPVS IQGSSVMFRT
160 170 180 190 200
VEVTLHKEGN TFGFVIRGGA HDDRNKSRPV VITCVRPGGP ADREGTIKPG
210 220 230 240 250
DRLLSVDGIR LLGTTHAEAM SILKQCGQEA TLLIEYDVSV MDSVATASGP
260 270 280 290 300
LLVEVAKTPG ASLGVALTTS VCCNKQVIVI DKIKSASIAD RCGALHVGDH
310 320 330 340 350
ILSIDGTSME YCTLAEATQF LANTTDQVKL EILPHHQTRL ALKGPDHVKI
360 370 380 390 400
QRSDRQLPWD PWASSQCSVH TNHHHNPHHP DHCRVPALGF PKALTPNSPP
410 420 430 440 450
AMVSSSSPTS MSAYSLSSLN MGTLPRSLYS TSPRGTMMRR RLKKKDFKSS
460 470 480 490 500
LSLASSTVGL AGQVVHTETT EVVLTADPVT GFGIQLQGSV FATETLSSPP
510 520 530 540 550
LISYIEADSP AERCGVLQIG DRVMAINGIP TEDSTFEEAN QLLRDSSITS
560 570 580 590 600
KVTLEIEFDV AESVIPSSGT FHVKLPKKHS VELGITISSP SSRKPGDPLV
610 620 630 640 650
ISDIKKGSVA HRTGTLELGD KLLAIDNIRL DSCSMEDAVQ ILQQCEDLVK
660 670 680 690 700
LKIRKDEDNS DEQESSGAII YTVELKRYGG PLGITISGTE EPFDPIIISS
710 720 730 740 750
LTKGGLAERT GAIHIGDRIL AINSSSLKGK PLSEAIHLLQ MAGETVTLKI
760 770 780 790 800
KKQTDAQPAS SPKKLPIPSH SSDLGDGEED PSPIQRPGKL SDVYPSTVPS
810 820 830 840 850
VDSAVDSWDG SGIDARYGSQ GTTFQTSGYN FNTYDWRSPK KRASLSPVPK
860 870 880 890 900
PRSQTYPDVG LSNEDWDRST ASGFAGASDS ADAEQEENFW SQALEDLETC
910 920 930 940 950
GQSGILRELE ATIMSGSTMS LNHEAPTARS QLGRQASFQE RSNSRPHYSQ
960 970 980 990 1000
TTRSNTLPSD VGRKSVTLRK MKQEIKEIMS PTPVELHKVT LYKDSGMEDF
1010 1020 1030 1040 1050
GFSVADGLLE KGVYVKNIRP AGPGDLGGLK PYDRLLQVNH VRTRDFDCCL
1060 1070 1080 1090 1100
VVPLIAESGN KLDLVISRNP LASQKSIEQP ALPSDWSEQN SAFFQQPSHG
1110
GNLETREPTN TL
The sequence of this isoform differs from the canonical sequence as follows:
1-416: Missing.
417-451: SSLNMGTLPRSLYSTSPRGTMMRRRLKKKDFKSSL → MTPKRTEKEMKKPHNFHHASHPPLRKGQKINAAHV
1101-1112: GNLETREPTNTL → IPGDAVYFWQS
Computationally mapped potential isoform sequencesi
There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketF1LRA4 | F1LRA4_RAT | Glutamate receptor-interacting prot... | Grip1 | 1,094 | Annotation score: | ||
F1LMX8 | F1LMX8_RAT | Glutamate receptor-interacting prot... | Grip1 | 1,096 | Annotation score: | ||
Q30A72 | Q30A72_RAT | Glutamate receptor interacting prot... | Grip1 | 711 | Annotation score: | ||
A0A140TA96 | A0A140TA96_RAT | Glutamate receptor-interacting prot... | Grip1 | 696 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 816 | R → S in AAR08916 (PubMed:15226318).Curated | 1 | |
Sequence conflicti | 841 | K → Q in AAR08916 (PubMed:15226318).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_009751 | 1 – 416 | Missing in isoform 2. 1 PublicationAdd BLAST | 416 | |
Alternative sequenceiVSP_009752 | 417 – 451 | SSLNM…FKSSL → MTPKRTEKEMKKPHNFHHAS HPPLRKGQKINAAHV in isoform 2. 1 PublicationAdd BLAST | 35 | |
Alternative sequenceiVSP_009753 | 1101 – 1112 | GNLET…PTNTL → IPGDAVYFWQS in isoform 2. 1 PublicationAdd BLAST | 12 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U88572 mRNA Translation: AAB51689.1 AY437398 mRNA Translation: AAR08916.1 |
PIRi | T32733 |
RefSeqi | NP_114458.1, NM_032069.1 [P97879-1] |
Genome annotation databases
GeneIDi | 84016 |
KEGGi | rno:84016 |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U88572 mRNA Translation: AAB51689.1 AY437398 mRNA Translation: AAR08916.1 |
PIRi | T32733 |
RefSeqi | NP_114458.1, NM_032069.1 [P97879-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1M5Z | NMR | - | A | 980-1070 | [»] | |
1N7E | X-ray | 1.50 | A | 665-761 | [»] | |
1N7F | X-ray | 1.80 | A/B | 665-761 | [»] | |
1P1D | NMR | - | A | 463-658 | [»] | |
1P1E | NMR | - | A | 463-563 | [»] | |
2QT5 | X-ray | 2.30 | A/B | 48-243 | [»] | |
SMRi | P97879 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 249882, 11 interactors |
CORUMi | P97879 |
DIPi | DIP-37486N |
ELMi | P97879 |
IntActi | P97879, 34 interactors |
MINTi | P97879 |
STRINGi | 10116.ENSRNOP00000005539 |
Chemistry databases
BindingDBi | P97879 |
ChEMBLi | CHEMBL2366484 |
PTM databases
iPTMneti | P97879 |
PhosphoSitePlusi | P97879 |
SwissPalmi | P97879 |
Proteomic databases
PaxDbi | P97879 |
Genome annotation databases
GeneIDi | 84016 |
KEGGi | rno:84016 |
Organism-specific databases
CTDi | 23426 |
RGDi | 621667, Grip1 |
Phylogenomic databases
eggNOGi | KOG3528, Eukaryota |
InParanoidi | P97879 |
OrthoDBi | 65191at2759 |
PhylomeDBi | P97879 |
Enzyme and pathway databases
Reactomei | R-RNO-416993, Trafficking of GluR2-containing AMPA receptors |
Miscellaneous databases
EvolutionaryTracei | P97879 |
PROi | PR:P97879 |
Family and domain databases
Gene3Di | 2.30.42.10, 7 hits |
InterProi | View protein in InterPro IPR030026, GRIP1 IPR043545, GRIP1/2 IPR001478, PDZ IPR041489, PDZ_6 IPR036034, PDZ_sf |
PANTHERi | PTHR46227, PTHR46227, 1 hit PTHR46227:SF3, PTHR46227:SF3, 1 hit |
Pfami | View protein in Pfam PF00595, PDZ, 6 hits PF17820, PDZ_6, 1 hit |
SMARTi | View protein in SMART SM00228, PDZ, 7 hits |
SUPFAMi | SSF50156, SSF50156, 7 hits |
PROSITEi | View protein in PROSITE PS50106, PDZ, 7 hits |
MobiDBi | Search... |
Entry informationi
Entry namei | GRIP1_RAT | |
Accessioni | P97879Primary (citable) accession number: P97879 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 29, 2004 |
Last sequence update: | May 1, 1997 | |
Last modified: | February 23, 2022 | |
This is version 167 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references