UniProtKB - P97864 (CASP7_MOUSE)
Protein
Caspase-7
Gene
Casp7
Organism
Mus musculus (Mouse)
Status
Functioni
Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Overexpression promotes programmed cell death (By similarity).By similarity
Catalytic activityi
- Strict requirement for an Asp residue at position P1 and has a preferred cleavage sequence of Asp-Glu-Val-Asp-|-. EC:3.4.22.60
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 144 | By similarity | 1 | |
Active sitei | 186 | By similarity | 1 |
GO - Molecular functioni
- aspartic-type endopeptidase activity Source: MGI
- cysteine-type endopeptidase activity Source: UniProtKB
- cysteine-type endopeptidase activity involved in apoptotic process Source: MGI
- cysteine-type endopeptidase activity involved in execution phase of apoptosis Source: MGI
- cysteine-type peptidase activity Source: MGI
- peptidase activity Source: MGI
GO - Biological processi
- aging Source: Ensembl
- apoptotic process Source: MGI
- cellular response to staurosporine Source: MGI
- execution phase of apoptosis Source: MGI
- heart development Source: MGI
- leukocyte apoptotic process Source: Ensembl
- neuron apoptotic process Source: MGI
- protein processing Source: MGI
- proteolysis Source: MGI
- response to UV Source: MGI
- striated muscle cell differentiation Source: Ensembl
Keywordsi
Molecular function | Hydrolase, Protease, Thiol protease |
Biological process | Apoptosis |
Enzyme and pathway databases
BRENDAi | 3.4.22.60, 3474 |
Reactomei | R-MMU-111459, Activation of caspases through apoptosome-mediated cleavage R-MMU-111463, SMAC (DIABLO) binds to IAPs R-MMU-111464, SMAC(DIABLO)-mediated dissociation of IAP:caspase complexes R-MMU-111465, Apoptotic cleavage of cellular proteins R-MMU-111469, SMAC, XIAP-regulated apoptotic response R-MMU-264870, Caspase-mediated cleavage of cytoskeletal proteins |
Protein family/group databases
MEROPSi | C14.004 |
Names & Taxonomyi
Protein namesi | Recommended name: Caspase-7 (EC:3.4.22.60)Short name: CASP-7 Alternative name(s): Apoptotic protease Mch-3 Cysteine protease LICE2 Cleaved into the following 2 chains: |
Gene namesi | Name:Casp7 Synonyms:Lice2, Mch3 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:109383, Casp7 |
Subcellular locationi
Other locations
Cytosol
- cytosol Source: MGI
Nucleus
- nucleus Source: MGI
Other locations
- cytoplasm Source: GO_Central
- intracellular membrane-bounded organelle Source: MGI
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
PropeptideiPRO_0000004624 | 1 – 23 | By similarityAdd BLAST | 23 | |
ChainiPRO_0000004625 | 24 – 198 | Caspase-7 subunit p20By similarityAdd BLAST | 175 | |
PropeptideiPRO_0000004626 | 199 – 206 | By similarity | 8 | |
ChainiPRO_0000004627 | 207 – 303 | Caspase-7 subunit p11By similarityAdd BLAST | 97 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionineCombined sources | 1 |
Post-translational modificationi
Cleavages by granzyme B or caspase-10 generate the two active subunits. Propeptide domains can also be cleaved efficiently by caspase-3. Active heterodimers between the small subunit of caspase-7 and the large subunit of caspase-3, and vice versa, also occur (By similarity).By similarity
Keywords - PTMi
Acetylation, ZymogenProteomic databases
EPDi | P97864 |
jPOSTi | P97864 |
PaxDbi | P97864 |
PeptideAtlasi | P97864 |
PRIDEi | P97864 |
PTM databases
iPTMneti | P97864 |
PhosphoSitePlusi | P97864 |
Expressioni
Tissue specificityi
Highly expressed in heart, lung, liver and kidney. Low levels in spleen, skeletal muscle and testis. No expression in the brain.
Gene expression databases
Bgeei | ENSMUSG00000025076, Expressed in pineal body and 261 other tissues |
ExpressionAtlasi | P97864, baseline and differential |
Genevisiblei | P97864, MM |
Interactioni
Subunit structurei
Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 20 kDa (p20) and a 11 kDa (p11) subunit.
Interacts with BIRC6/bruce (By similarity).
Interacts with HSPA5 (By similarity).
By similarityBinary interactionsi
Hide detailsProtein-protein interaction databases
BioGRIDi | 198499, 7 interactors |
ComplexPortali | CPX-3947, Caspase-7 complex |
IntActi | P97864, 10 interactors |
STRINGi | 10090.ENSMUSP00000026062 |
Miscellaneous databases
RNActi | P97864, protein |
Family & Domainsi
Sequence similaritiesi
Belongs to the peptidase C14A family.Curated
Phylogenomic databases
eggNOGi | KOG3573, Eukaryota |
GeneTreei | ENSGT00940000153232 |
HOGENOMi | CLU_036904_2_0_1 |
InParanoidi | P97864 |
OMAi | SWRNTTR |
OrthoDBi | 984395at2759 |
PhylomeDBi | P97864 |
TreeFami | TF102023 |
Family and domain databases
CDDi | cd00032, CASc, 1 hit |
InterProi | View protein in InterPro IPR015471, Casp3/7 IPR029030, Caspase-like_dom_sf IPR033139, Caspase_cys_AS IPR016129, Caspase_his_AS IPR002398, Pept_C14 IPR002138, Pept_C14_p10 IPR001309, Pept_C14_p20 IPR015917, Pept_C14A |
PANTHERi | PTHR10454, PTHR10454, 1 hit PTHR10454:SF31, PTHR10454:SF31, 1 hit |
PRINTSi | PR00376, IL1BCENZYME |
SMARTi | View protein in SMART SM00115, CASc, 1 hit |
SUPFAMi | SSF52129, SSF52129, 1 hit |
PROSITEi | View protein in PROSITE PS01122, CASPASE_CYS, 1 hit PS01121, CASPASE_HIS, 1 hit PS50207, CASPASE_P10, 1 hit PS50208, CASPASE_P20, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
P97864-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTDDQDCAAE LEKVDSSSED GVDAKPDRSS IISSILLKKK RNASAGPVRT
60 70 80 90 100
GRDRVPTYLY RMDFQKMGKC IIINNKNFDK ATGMDVRNGT DKDAGALFKC
110 120 130 140 150
FQNLGFEVTV HNDCSCAKMQ DLLRKASEED HSNSACFACV LLSHGEEDLI
160 170 180 190 200
YGKDGVTPIK DLTAHFRGDR CKTLLEKPKL FFIQACRGTE LDDGIQADSG
210 220 230 240 250
PINDIDANPR NKIPVEADFL FAYSTVPGYY SWRNPGKGSW FVQALCSILN
260 270 280 290 300
EHGKDLEIMQ ILTRVNDRVA RHFESQSDDP RFNEKKQIPC MVSMLTKELY
FSR
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A5F8MPP6 | A0A5F8MPP6_MOUSE | Caspase-7 | Casp7 | 237 | Annotation score: |
Sequence cautioni
The sequence AAC53068 differs from that shown. Reason: Erroneous initiation.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 10 – 11 | EL → DW in BAA19730 (PubMed:9125129).Curated | 2 | |
Sequence conflicti | 45 | A → T in BAA19730 (PubMed:9125129).Curated | 1 | |
Sequence conflicti | 48 – 49 | VR → RQ in BAA19730 (PubMed:9125129).Curated | 2 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U67321 mRNA Translation: AAC53068.1 Different initiation. D86353 mRNA Translation: BAA19730.1 Y13088 mRNA Translation: CAA73530.1 BC005428 mRNA Translation: AAH05428.1 |
CCDSi | CCDS29915.1 |
RefSeqi | NP_031637.1, NM_007611.2 XP_006526679.1, XM_006526616.3 |
Genome annotation databases
Ensembli | ENSMUST00000026062; ENSMUSP00000026062; ENSMUSG00000025076 |
GeneIDi | 12369 |
KEGGi | mmu:12369 |
UCSCi | uc008hyw.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U67321 mRNA Translation: AAC53068.1 Different initiation. D86353 mRNA Translation: BAA19730.1 Y13088 mRNA Translation: CAA73530.1 BC005428 mRNA Translation: AAH05428.1 |
CCDSi | CCDS29915.1 |
RefSeqi | NP_031637.1, NM_007611.2 XP_006526679.1, XM_006526616.3 |
3D structure databases
SMRi | P97864 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 198499, 7 interactors |
ComplexPortali | CPX-3947, Caspase-7 complex |
IntActi | P97864, 10 interactors |
STRINGi | 10090.ENSMUSP00000026062 |
Protein family/group databases
MEROPSi | C14.004 |
PTM databases
iPTMneti | P97864 |
PhosphoSitePlusi | P97864 |
Proteomic databases
EPDi | P97864 |
jPOSTi | P97864 |
PaxDbi | P97864 |
PeptideAtlasi | P97864 |
PRIDEi | P97864 |
Protocols and materials databases
Antibodypediai | 18528, 1028 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000026062; ENSMUSP00000026062; ENSMUSG00000025076 |
GeneIDi | 12369 |
KEGGi | mmu:12369 |
UCSCi | uc008hyw.1, mouse |
Organism-specific databases
CTDi | 840 |
MGIi | MGI:109383, Casp7 |
Phylogenomic databases
eggNOGi | KOG3573, Eukaryota |
GeneTreei | ENSGT00940000153232 |
HOGENOMi | CLU_036904_2_0_1 |
InParanoidi | P97864 |
OMAi | SWRNTTR |
OrthoDBi | 984395at2759 |
PhylomeDBi | P97864 |
TreeFami | TF102023 |
Enzyme and pathway databases
BRENDAi | 3.4.22.60, 3474 |
Reactomei | R-MMU-111459, Activation of caspases through apoptosome-mediated cleavage R-MMU-111463, SMAC (DIABLO) binds to IAPs R-MMU-111464, SMAC(DIABLO)-mediated dissociation of IAP:caspase complexes R-MMU-111465, Apoptotic cleavage of cellular proteins R-MMU-111469, SMAC, XIAP-regulated apoptotic response R-MMU-264870, Caspase-mediated cleavage of cytoskeletal proteins |
Miscellaneous databases
BioGRID-ORCSi | 12369, 1 hit in 17 CRISPR screens |
ChiTaRSi | Casp7, mouse |
PROi | PR:P97864 |
RNActi | P97864, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000025076, Expressed in pineal body and 261 other tissues |
ExpressionAtlasi | P97864, baseline and differential |
Genevisiblei | P97864, MM |
Family and domain databases
CDDi | cd00032, CASc, 1 hit |
InterProi | View protein in InterPro IPR015471, Casp3/7 IPR029030, Caspase-like_dom_sf IPR033139, Caspase_cys_AS IPR016129, Caspase_his_AS IPR002398, Pept_C14 IPR002138, Pept_C14_p10 IPR001309, Pept_C14_p20 IPR015917, Pept_C14A |
PANTHERi | PTHR10454, PTHR10454, 1 hit PTHR10454:SF31, PTHR10454:SF31, 1 hit |
PRINTSi | PR00376, IL1BCENZYME |
SMARTi | View protein in SMART SM00115, CASc, 1 hit |
SUPFAMi | SSF52129, SSF52129, 1 hit |
PROSITEi | View protein in PROSITE PS01122, CASPASE_CYS, 1 hit PS01121, CASPASE_HIS, 1 hit PS50207, CASPASE_P10, 1 hit PS50208, CASPASE_P20, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CASP7_MOUSE | |
Accessioni | P97864Primary (citable) accession number: P97864 Secondary accession number(s): O08669 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | November 1, 1997 | |
Last modified: | February 10, 2021 | |
This is version 182 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families - Peptidase families
Classification of peptidase families and list of entries