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Protein

Nuclear factor 1 B-type

Gene

Nfib

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi1 – 195CTF/NF-IPROSITE-ProRule annotationAdd BLAST195

GO - Molecular functioni

GO - Biological processi

  • anterior commissure morphogenesis Source: UniProtKB
  • cell differentiation involved in salivary gland development Source: MGI
  • chondrocyte differentiation Source: UniProtKB
  • Clara cell differentiation Source: UniProtKB
  • commissural neuron axon guidance Source: UniProtKB
  • DNA replication Source: UniProtKB-KW
  • forebrain development Source: MGI
  • glial cell differentiation Source: UniProtKB
  • hindbrain development Source: MGI
  • lung ciliated cell differentiation Source: UniProtKB
  • lung development Source: MGI
  • negative regulation of cell proliferation Source: MGI
  • negative regulation of DNA binding Source: MGI
  • negative regulation of epithelial cell proliferation involved in lung morphogenesis Source: UniProtKB
  • negative regulation of mesenchymal cell proliferation involved in lung development Source: UniProtKB
  • negative regulation of pri-miRNA transcription by RNA polymerase II Source: MGI
  • negative regulation of transcription by RNA polymerase II Source: MGI
  • positive regulation of transcription, DNA-templated Source: MGI
  • positive regulation of transcription by RNA polymerase II Source: UniProtKB
  • principal sensory nucleus of trigeminal nerve development Source: UniProtKB
  • salivary gland cavitation Source: MGI
  • Type II pneumocyte differentiation Source: UniProtKB
  • Type I pneumocyte differentiation Source: UniProtKB

Keywordsi

Molecular functionActivator, DNA-binding
Biological processDNA replication, Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Nuclear factor 1 B-type
Short name:
NF1-B
Short name:
Nuclear factor 1/B
Alternative name(s):
CCAAT-box-binding transcription factor
Short name:
CTF
Nuclear factor I/B
Short name:
NF-I/B
Short name:
NFI-B
TGGCA-binding protein
Gene namesi
Name:Nfib
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:103188 Nfib

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001001971 – 570Nuclear factor 1 B-typeAdd BLAST570

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei264PhosphoserineBy similarity1
Modified residuei286PhosphothreonineCombined sources1
Modified residuei292PhosphoserineCombined sources1
Modified residuei295PhosphoserineCombined sources1
Modified residuei312PhosphoserineCombined sources1
Modified residuei328PhosphoserineBy similarity1
Modified residuei333PhosphoserineBy similarity1
Modified residuei335Asymmetric dimethylarginineCombined sources1
Modified residuei388Asymmetric dimethylarginineCombined sources1

Keywords - PTMi

Methylation, Phosphoprotein

Proteomic databases

MaxQBiP97863
PaxDbiP97863
PRIDEiP97863

PTM databases

iPTMnetiP97863
PhosphoSitePlusiP97863

Expressioni

Tissue specificityi

Highest expression in lung, skeletal muscle and heart. Lower levels in liver, kidney and brain. Very low levels in testis and spleen.

Gene expression databases

BgeeiENSMUSG00000008575
CleanExiMM_NFIB
ExpressionAtlasiP97863 baseline and differential
GenevisibleiP97863 MM

Interactioni

Subunit structurei

Binds DNA as a homodimer.

Protein-protein interaction databases

BioGridi201747, 2 interactors
IntActiP97863, 2 interactors
MINTiP97863
STRINGi10090.ENSMUSP00000052863

Structurei

3D structure databases

ProteinModelPortaliP97863
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the CTF/NF-I family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG3663 Eukaryota
ENOG410Y16G LUCA
GeneTreeiENSGT00390000009905
HOGENOMiHOG000013028
HOVERGENiHBG006561
InParanoidiP97863
KOiK09169
OMAiSPSNNEP
OrthoDBiEOG091G0HVY
PhylomeDBiP97863
TreeFamiTF313889

Family and domain databases

InterProiView protein in InterPro
IPR000647 CTF/NFI
IPR020604 CTF/NFI_DNA-bd-dom
IPR019739 CTF/NFI_DNA-bd_CS
IPR019548 CTF/NFI_DNA-bd_N
IPR003619 MAD_homology1_Dwarfin-type
PANTHERiPTHR11492 PTHR11492, 1 hit
PfamiView protein in Pfam
PF00859 CTF_NFI, 1 hit
PF03165 MH1, 1 hit
PF10524 NfI_DNAbd_pre-N, 1 hit
SMARTiView protein in SMART
SM00523 DWA, 1 hit
PROSITEiView protein in PROSITE
PS00349 CTF_NFI_1, 1 hit
PS51080 CTF_NFI_2, 1 hit

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P97863-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MMYSPICLTQ DEFHPFIEAL LPHVRAIAYT WFNLQARKRK YFKKHEKRMS
60 70 80 90 100
KDEERAVKDE LLSEKPEIKQ KWASRLLAKL RKDIRQEYRE DFVLTVTGKK
110 120 130 140 150
HPCCVLSNPD QKGKIRRIDC LRQADKVWRL DLVMVILFKG IPLESTDGER
160 170 180 190 200
LMKSPHCTNP ALCVQPHHIT VSVKELDLFL AYYVQEQDSG QSGSPSHSDP
210 220 230 240 250
AKNPPGYLED SFVKSGVFNV SELVRVSRTP ITQGTGVNFP IGEIPSQPYY
260 270 280 290 300
HDMNSGVNLQ RSLSSPPSSK RPKTISIDEN MEPSPTGDFY PSPNSPAAGS
310 320 330 340 350
RTWHERDQDM SSPTTMKKPE KPLFSSTSPQ DSSPRLSTFP QHHHPGIPGV
360 370 380 390 400
AHSVISTRTP PPPSPLPFPT QAILPPAPSS YFSHPTIRYP PHLNPQDTLK
410 420 430 440 450
NYVPSYDPSS PQTSQPNSSG QVVGKVPGHF TPVLAPSPHP SAVRPVTLTM
460 470 480 490 500
TDTKPITTST EGEAASPTAT TYTASGTSQA NRYVGLSPRD PSFLHQQQLR
510 520 530 540 550
ICDWTMNQNG RHLYPSTSED TLGITWQSPG TWASLVPFQV SNRTPILPAN
560 570
VQNYGLNIIG EPFLQAETSN
Length:570
Mass (Da):63,507
Last modified:June 1, 2001 - v2
Checksum:iD94AB4400A2811CC
GO
Isoform 2 (identifier: P97863-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     416-498: Missing.

Show »
Length:487
Mass (Da):54,908
Checksum:i868043916A36234F
GO
Isoform 3 (identifier: P97863-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     416-420: PNSSG → SWYLG
     421-570: Missing.

Show »
Length:420
Mass (Da):47,472
Checksum:iE26259E1F965146D
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti131D → G in strain: NIH Swiss. 1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_003547416 – 498Missing in isoform 2. CuratedAdd BLAST83
Alternative sequenceiVSP_003548416 – 420PNSSG → SWYLG in isoform 3. 1 Publication5
Alternative sequenceiVSP_003549421 – 570Missing in isoform 3. 1 PublicationAdd BLAST150

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U57634 mRNA Translation: AAB49929.1
Y07685 mRNA Translation: CAA68949.1
Y07686 mRNA Translation: CAA68950.1
Y07687 mRNA Translation: CAA68951.1
BC014290 mRNA Translation: AAH14290.1
AF111264 Genomic DNA Translation: AAD39099.1
CCDSiCCDS38790.1 [P97863-3]
CCDS51213.1 [P97863-1]
RefSeqiNP_001106680.1, NM_001113209.2 [P97863-1]
NP_032713.3, NM_008687.6 [P97863-3]
UniGeneiMm.317947

Genome annotation databases

EnsembliENSMUST00000050872; ENSMUSP00000052863; ENSMUSG00000008575 [P97863-1]
ENSMUST00000064770; ENSMUSP00000067629; ENSMUSG00000008575 [P97863-3]
GeneIDi18028
KEGGimmu:18028
UCSCiuc008tjz.2 mouse [P97863-1]
uc008tkd.3 mouse [P97863-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiNFIB_MOUSE
AccessioniPrimary (citable) accession number: P97863
Secondary accession number(s): P70252
, P70253, P70254, Q9R1G4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: June 1, 2001
Last modified: July 18, 2018
This is version 161 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

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