UniProtKB - P97857 (ATS1_MOUSE)
A disintegrin and metalloproteinase with thrombospondin motifs 1
Adamts1
Functioni
Cleaves aggrecan, a cartilage proteoglycan, at the '1691-Glu-|-Leu-1692' site (within the chondroitin sulfate attachment domain), and may be involved in its turnover. Has angiogenic inhibitor activity (By similarity).
Active metalloprotease, which may be associated with various inflammatory processes as well as development of cancer cachexia. May play a critical role in follicular rupture (By similarity).
By similarity2 PublicationsCofactori
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 206 | Zinc; in inhibited formBy similarity | 1 | |
Metal bindingi | 262 | Calcium 1By similarity | 1 | |
Metal bindingi | 262 | Calcium 2By similarity | 1 | |
Metal bindingi | 345 | Calcium 1By similarity | 1 | |
Metal bindingi | 345 | Calcium 2; via carbonyl oxygenBy similarity | 1 | |
Metal bindingi | 352 | Calcium 1By similarity | 1 | |
Metal bindingi | 402 | Zinc; catalyticBy similarity | 1 | |
Active sitei | 403 | 1 Publication | 1 | |
Metal bindingi | 406 | Zinc; catalyticBy similarity | 1 | |
Metal bindingi | 412 | Zinc; catalyticBy similarity | 1 | |
Metal bindingi | 463 | Calcium 1; via carbonyl oxygenBy similarity | 1 | |
Metal bindingi | 466 | Calcium 1By similarity | 1 | |
Metal bindingi | 466 | Calcium 2By similarity | 1 |
GO - Molecular functioni
- heparin binding Source: MGI
- metalloendopeptidase activity Source: MGI
- zinc ion binding Source: InterPro
GO - Biological processi
- extracellular matrix organization Source: GO_Central
- heart trabecula formation Source: MGI
- kidney development Source: MGI
- negative regulation of angiogenesis Source: MGI
- ovulation from ovarian follicle Source: MGI
- positive regulation of G1/S transition of mitotic cell cycle Source: MGI
- positive regulation of neuron projection development Source: MGI
- positive regulation of vascular associated smooth muscle cell migration Source: MGI
- positive regulation of vascular associated smooth muscle cell proliferation Source: MGI
Keywordsi
Molecular function | Heparin-binding, Hydrolase, Metalloprotease, Protease |
Ligand | Calcium, Metal-binding, Zinc |
Enzyme and pathway databases
BRENDAi | 3.4.24.B11, 3474 |
Reactomei | R-MMU-5173214, O-glycosylation of TSR domain-containing proteins |
Protein family/group databases
MEROPSi | M12.222 |
Names & Taxonomyi
Protein namesi | Recommended name: A disintegrin and metalloproteinase with thrombospondin motifs 1 (EC:3.4.24.-)Short name: ADAM-TS 1 Short name: ADAM-TS1 Short name: ADAMTS-1 |
Gene namesi | Name:Adamts1 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:109249, Adamts1 |
VEuPathDBi | HostDB:ENSMUSG00000022893 |
Subcellular locationi
Extracellular region or secreted
Extracellular region or secreted
- extracellular region Source: UniProtKB-KW
Other locations
- basement membrane Source: MGI
- collagen-containing extracellular matrix Source: MGI
- cytoplasmic vesicle Source: MGI
- extracellular matrix Source: MGI
Keywords - Cellular componenti
Extracellular matrix, SecretedPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 403 | E → Q: Loss of activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 48 | Sequence analysisAdd BLAST | 48 | |
PropeptideiPRO_0000029152 | 49 – 253 | Add BLAST | 205 | |
ChainiPRO_0000029153 | 254 – 968 | A disintegrin and metalloproteinase with thrombospondin motifs 1Add BLAST | 715 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 334 ↔ 386 | By similarity | ||
Disulfide bondi | 363 ↔ 368 | By similarity | ||
Disulfide bondi | 380 ↔ 463 | By similarity | ||
Disulfide bondi | 418 ↔ 447 | By similarity | ||
Disulfide bondi | 489 ↔ 512 | By similarity | ||
Disulfide bondi | 500 ↔ 522 | By similarity | ||
Disulfide bondi | 507 ↔ 541 | By similarity | ||
Disulfide bondi | 535 ↔ 546 | By similarity | ||
Glycosylationi | 548 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 572 ↔ 609 | By similarity | ||
Disulfide bondi | 576 ↔ 614 | By similarity | ||
Disulfide bondi | 587 ↔ 599 | By similarity | ||
Glycosylationi | 721 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 765 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 783 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 946 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Post-translational modificationi
Keywords - PTMi
Cleavage on pair of basic residues, Disulfide bond, Glycoprotein, ZymogenProteomic databases
CPTACi | non-CPTAC-3962 |
MaxQBi | P97857 |
PaxDbi | P97857 |
PeptideAtlasi | P97857 |
PRIDEi | P97857 |
ProteomicsDBi | 277135 |
PTM databases
GlyGeni | P97857, 5 sites |
iPTMneti | P97857 |
PhosphoSitePlusi | P97857 |
Expressioni
Developmental stagei
Inductioni
Gene expression databases
Bgeei | ENSMUSG00000022893, Expressed in pancreas and 312 other tissues |
ExpressionAtlasi | P97857, baseline and differential |
Genevisiblei | P97857, MM |
Interactioni
Protein-protein interaction databases
BioGRIDi | 197974, 4 interactors |
STRINGi | 10090.ENSMUSP00000023610 |
Miscellaneous databases
RNActi | P97857, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 259 – 468 | Peptidase M12BPROSITE-ProRule annotationAdd BLAST | 210 | |
Domaini | 477 – 559 | DisintegrinAdd BLAST | 83 | |
Domaini | 560 – 615 | TSP type-1 1PROSITE-ProRule annotationAdd BLAST | 56 | |
Domaini | 855 – 911 | TSP type-1 2PROSITE-ProRule annotationAdd BLAST | 57 | |
Domaini | 912 – 968 | TSP type-1 3PROSITE-ProRule annotationAdd BLAST | 57 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 23 | DisorderedSequence analysisAdd BLAST | 23 | |
Regioni | 177 – 253 | DisorderedSequence analysisAdd BLAST | 77 | |
Regioni | 726 – 850 | SpacerAdd BLAST | 125 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 204 – 211 | Cysteine switchBy similarity | 8 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 218 – 233 | Polar residuesSequence analysisAdd BLAST | 16 |
Domaini
Keywords - Domaini
Repeat, SignalPhylogenomic databases
eggNOGi | KOG3538, Eukaryota |
GeneTreei | ENSGT00940000156815 |
HOGENOMi | CLU_000660_3_0_1 |
InParanoidi | P97857 |
OMAi | KKHFDTP |
OrthoDBi | 125522at2759 |
PhylomeDBi | P97857 |
TreeFami | TF331949 |
Family and domain databases
Gene3Di | 2.20.100.10, 3 hits 3.40.390.10, 1 hit |
InterProi | View protein in InterPro IPR006586, ADAM_Cys-rich IPR013273, ADAMTS/ADAMTS-like IPR041645, ADAMTS_CR_2 IPR045371, ADAMTS_CR_3 IPR010294, ADAMTS_spacer1 IPR024079, MetalloPept_cat_dom_sf IPR013274, Pept_M12B_ADAM-TS1 IPR001590, Peptidase_M12B IPR002870, Peptidase_M12B_N IPR000884, TSP1_rpt IPR036383, TSP1_rpt_sf |
Pfami | View protein in Pfam PF17771, ADAM_CR_2, 1 hit PF19236, ADAM_CR_3, 1 hit PF05986, ADAM_spacer1, 1 hit PF01562, Pep_M12B_propep, 1 hit PF01421, Reprolysin, 1 hit PF00090, TSP_1, 1 hit |
PRINTSi | PR01858, ADAMTS1 PR01857, ADAMTSFAMILY |
SMARTi | View protein in SMART SM00608, ACR, 1 hit SM00209, TSP1, 3 hits |
SUPFAMi | SSF82895, SSF82895, 3 hits |
PROSITEi | View protein in PROSITE PS50215, ADAM_MEPRO, 1 hit PS50092, TSP1, 3 hits PS00142, ZINC_PROTEASE, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
10 20 30 40 50
MQPKVPLGSR KQKPCSDMGD VQRAARSRGS LSAHMLLLLL ASITMLLCAR
60 70 80 90 100
GAHGRPTEED EELVLPSLER APGHDSTTTR LRLDAFGQQL HLKLQPDSGF
110 120 130 140 150
LAPGFTLQTV GRSPGSEAQH LDPTGDLAHC FYSGTVNGDP GSAAALSLCE
160 170 180 190 200
GVRGAFYLQG EEFFIQPAPG VATERLAPAV PEEESSARPQ FHILRRRRRG
210 220 230 240 250
SGGAKCGVMD DETLPTSDSR PESQNTRNQW PVRDPTPQDA GKPSGPGSIR
260 270 280 290 300
KKRFVSSPRY VETMLVADQS MADFHGSGLK HYLLTLFSVA ARFYKHPSIR
310 320 330 340 350
NSISLVVVKI LVIYEEQKGP EVTSNAALTL RNFCSWQKQH NSPSDRDPEH
360 370 380 390 400
YDTAILFTRQ DLCGSHTCDT LGMADVGTVC DPSRSCSVIE DDGLQAAFTT
410 420 430 440 450
AHELGHVFNM PHDDAKHCAS LNGVSGDSHL MASMLSSLDH SQPWSPCSAY
460 470 480 490 500
MVTSFLDNGH GECLMDKPQN PIKLPSDLPG TLYDANRQCQ FTFGEESKHC
510 520 530 540 550
PDAASTCTTL WCTGTSGGLL VCQTKHFPWA DGTSCGEGKW CVSGKCVNKT
560 570 580 590 600
DMKHFATPVH GSWGPWGPWG DCSRTCGGGV QYTMRECDNP VPKNGGKYCE
610 620 630 640 650
GKRVRYRSCN IEDCPDNNGK TFREEQCEAH NEFSKASFGN EPTVEWTPKY
660 670 680 690 700
AGVSPKDRCK LTCEAKGIGY FFVLQPKVVD GTPCSPDSTS VCVQGQCVKA
710 720 730 740 750
GCDRIIDSKK KFDKCGVCGG NGSTCKKMSG IVTSTRPGYH DIVTIPAGAT
760 770 780 790 800
NIEVKHRNQR GSRNNGSFLA IRAADGTYIL NGNFTLSTLE QDLTYKGTVL
810 820 830 840 850
RYSGSSAALE RIRSFSPLKE PLTIQVLMVG HALRPKIKFT YFMKKKTESF
860 870 880 890 900
NAIPTFSEWV IEEWGECSKT CGSGWQRRVV QCRDINGHPA SECAKEVKPA
910 920 930 940 950
STRPCADLPC PHWQVGDWSP CSKTCGKGYK KRTLKCVSHD GGVLSNESCD
960
PLKKPKHYID FCTLTQCS
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketE9PY08 | E9PY08_MOUSE | A disintegrin and metalloproteinase... | Adamts1 | 681 | Annotation score: |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 335 | S → N in BAA24501 (PubMed:9441751).Curated | 1 | |
Sequence conflicti | 335 | S → N in AAH40382 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 335 | S → N in AAH50834 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 425 | S → T in BAA24501 (PubMed:9441751).Curated | 1 | |
Sequence conflicti | 425 | S → T in AAH40382 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 425 | S → T in AAH50834 (PubMed:15489334).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB001735 Genomic DNA Translation: BAA24501.1 Different initiation. D67076 mRNA Translation: BAA11088.1 Frameshift. AC126936 Genomic DNA No translation available. BC040382 mRNA Translation: AAH40382.1 BC050834 mRNA Translation: AAH50834.1 |
CCDSi | CCDS28287.1 |
PIRi | T00017 |
RefSeqi | NP_033751.3, NM_009621.5 |
Genome annotation databases
Ensembli | ENSMUST00000023610; ENSMUSP00000023610; ENSMUSG00000022893 |
GeneIDi | 11504 |
KEGGi | mmu:11504 |
UCSCi | uc012aho.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB001735 Genomic DNA Translation: BAA24501.1 Different initiation. D67076 mRNA Translation: BAA11088.1 Frameshift. AC126936 Genomic DNA No translation available. BC040382 mRNA Translation: AAH40382.1 BC050834 mRNA Translation: AAH50834.1 |
CCDSi | CCDS28287.1 |
PIRi | T00017 |
RefSeqi | NP_033751.3, NM_009621.5 |
3D structure databases
SMRi | P97857 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 197974, 4 interactors |
STRINGi | 10090.ENSMUSP00000023610 |
Protein family/group databases
MEROPSi | M12.222 |
PTM databases
GlyGeni | P97857, 5 sites |
iPTMneti | P97857 |
PhosphoSitePlusi | P97857 |
Proteomic databases
CPTACi | non-CPTAC-3962 |
MaxQBi | P97857 |
PaxDbi | P97857 |
PeptideAtlasi | P97857 |
PRIDEi | P97857 |
ProteomicsDBi | 277135 |
Protocols and materials databases
Antibodypediai | 4291, 409 antibodies from 33 providers |
DNASUi | 11504 |
Genome annotation databases
Ensembli | ENSMUST00000023610; ENSMUSP00000023610; ENSMUSG00000022893 |
GeneIDi | 11504 |
KEGGi | mmu:11504 |
UCSCi | uc012aho.1, mouse |
Organism-specific databases
CTDi | 9510 |
MGIi | MGI:109249, Adamts1 |
VEuPathDBi | HostDB:ENSMUSG00000022893 |
Phylogenomic databases
eggNOGi | KOG3538, Eukaryota |
GeneTreei | ENSGT00940000156815 |
HOGENOMi | CLU_000660_3_0_1 |
InParanoidi | P97857 |
OMAi | KKHFDTP |
OrthoDBi | 125522at2759 |
PhylomeDBi | P97857 |
TreeFami | TF331949 |
Enzyme and pathway databases
BRENDAi | 3.4.24.B11, 3474 |
Reactomei | R-MMU-5173214, O-glycosylation of TSR domain-containing proteins |
Miscellaneous databases
BioGRID-ORCSi | 11504, 0 hits in 65 CRISPR screens |
ChiTaRSi | Adamts1, mouse |
PROi | PR:P97857 |
RNActi | P97857, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000022893, Expressed in pancreas and 312 other tissues |
ExpressionAtlasi | P97857, baseline and differential |
Genevisiblei | P97857, MM |
Family and domain databases
Gene3Di | 2.20.100.10, 3 hits 3.40.390.10, 1 hit |
InterProi | View protein in InterPro IPR006586, ADAM_Cys-rich IPR013273, ADAMTS/ADAMTS-like IPR041645, ADAMTS_CR_2 IPR045371, ADAMTS_CR_3 IPR010294, ADAMTS_spacer1 IPR024079, MetalloPept_cat_dom_sf IPR013274, Pept_M12B_ADAM-TS1 IPR001590, Peptidase_M12B IPR002870, Peptidase_M12B_N IPR000884, TSP1_rpt IPR036383, TSP1_rpt_sf |
Pfami | View protein in Pfam PF17771, ADAM_CR_2, 1 hit PF19236, ADAM_CR_3, 1 hit PF05986, ADAM_spacer1, 1 hit PF01562, Pep_M12B_propep, 1 hit PF01421, Reprolysin, 1 hit PF00090, TSP_1, 1 hit |
PRINTSi | PR01858, ADAMTS1 PR01857, ADAMTSFAMILY |
SMARTi | View protein in SMART SM00608, ACR, 1 hit SM00209, TSP1, 3 hits |
SUPFAMi | SSF82895, SSF82895, 3 hits |
PROSITEi | View protein in PROSITE PS50215, ADAM_MEPRO, 1 hit PS50092, TSP1, 3 hits PS00142, ZINC_PROTEASE, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ATS1_MOUSE | |
Accessioni | P97857Primary (citable) accession number: P97857 Secondary accession number(s): E9QMN9, O54768 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 30, 2000 |
Last sequence update: | July 27, 2011 | |
Last modified: | February 23, 2022 | |
This is version 184 of the entry and version 4 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot