UniProtKB - P97855 (G3BP1_MOUSE)
Ras GTPase-activating protein-binding protein 1
G3bp1
Functioni
Catalytic activityi
Cofactori
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- DNA binding Source: UniProtKB-KW
- DNA helicase activity Source: UniProtKB-EC
- endonuclease activity Source: UniProtKB-KW
- mRNA binding Source: MGI
- RNA helicase activity Source: UniProtKB-EC
GO - Biological processi
- defense response to virus Source: MGI
- innate immune response Source: UniProtKB-KW
- negative regulation of canonical Wnt signaling pathway Source: MGI
- positive regulation of stress granule assembly Source: MGI
- stress granule assembly Source: MGI
- type I interferon production Source: MGI
Keywordsi
Molecular function | DNA-binding, Endonuclease, Helicase, Hydrolase, Nuclease, RNA-binding |
Biological process | Immunity, Innate immunity, Transport |
Ligand | ATP-binding, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:G3bp1 Synonyms:G3bp |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1351465 G3bp1 |
Subcellular locationi
Nucleus
- Nucleus By similarity
Cytosol
- cytosol 2 Publications
Other locations
- Perikaryon 1 Publication
- Stress granule By similarity
Note: Cytoplasmic in proliferating cells, can be recruited to the plasma membrane in exponentially growing cells. Cytosolic and partially nuclear in resting cells. Recruited to stress granules in response to arsenite treatment. The unphosphorylated form is recruited to stress granules. HRAS signaling contributes to this process by regulating G3BP dephosphorylation.By similarity
Cytosol
- cytosol Source: MGI
Nucleus
- nucleus Source: UniProtKB-SubCell
Other locations
- cytoplasm Source: MGI
- cytoplasmic stress granule Source: ARUK-UCL
- perikaryon Source: UniProtKB-SubCell
- ribonucleoprotein complex Source: GO_Central
Keywords - Cellular componenti
Cytoplasm, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000194799 | 1 – 465 | Ras GTPase-activating protein-binding protein 1Add BLAST | 465 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 143 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 149 | PhosphoserineCombined sources1 Publication | 1 | |
Modified residuei | 231 | PhosphoserineCombined sources1 Publication | 1 | |
Modified residuei | 248 | PhosphoserineBy similarity | 1 | |
Modified residuei | 251 | PhosphoserineBy similarity | 1 | |
Modified residuei | 371 | PhosphoserineBy similarity | 1 | |
Modified residuei | 374 | N6-acetyllysine; alternateBy similarity | 1 | |
Cross-linki | 374 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity | ||
Modified residuei | 427 | Asymmetric dimethylarginineBy similarity | 1 | |
Modified residuei | 433 | Asymmetric dimethylarginine; alternateBy similarity | 1 | |
Modified residuei | 433 | Dimethylated arginine; alternateBy similarity | 1 | |
Modified residuei | 433 | Omega-N-methylarginine; alternateCombined sources | 1 | |
Modified residuei | 445 | Omega-N-methylarginine; alternateCombined sources | 1 | |
Modified residuei | 445 | Omega-N-methylated arginine; alternateBy similarity | 1 | |
Modified residuei | 458 | Dimethylated arginine; alternateBy similarity | 1 | |
Modified residuei | 458 | Omega-N-methylarginine; alternateCombined sources | 1 | |
Modified residuei | 458 | Omega-N-methylated arginine; alternateBy similarity | 1 | |
Modified residuei | 464 | Omega-N-methylarginine; alternateBy similarity | 1 | |
Modified residuei | 464 | Omega-N-methylated arginine; alternateBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Acetylation, Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | P97855 |
jPOSTi | P97855 |
PaxDbi | P97855 |
PeptideAtlasi | P97855 |
PRIDEi | P97855 |
PTM databases
iPTMneti | P97855 |
PhosphoSitePlusi | P97855 |
SwissPalmi | P97855 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSMUSG00000018583 Expressed in 317 organ(s), highest expression level in trachea |
Genevisiblei | P97855 MM |
Interactioni
Subunit structurei
Homodimer and oligomer (By similarity).
Component of a TAU mRNP complex, at least composed of IGF2BP1, ELAVL4 and G3BP1 (PubMed:15086518). Binds to the SH3 domain of Ras GTPase-activating protein (RASA1) in proliferating cells (PubMed:8649363). No interaction in quiescent cells (By similarity).
Interacts (via NTF2-like domain) with USP10.
Interacts with RPTOR and SPAG5; this complex is increased by oxidative stress.
Interacts with ATXN2L.
Interacts with STYXL1.
Interacts with CGAS (via N-terminus); this interaction promotes the DNA-binding and activation of CGAS.
Interacts (via C-terminus) with DDX58.
Interacts (via NTF2-like domain) with CAPRIN1.
Interacts with PABPC1 (By similarity).
By similarity1 PublicationProtein-protein interaction databases
BioGridi | 205102, 10 interactors |
IntActi | P97855, 11 interactors |
MINTi | P97855 |
STRINGi | 10090.ENSMUSP00000018727 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 11 – 133 | NTF2PROSITE-ProRule annotationAdd BLAST | 123 | |
Domaini | 338 – 413 | RRMPROSITE-ProRule annotationAdd BLAST | 76 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 144 – 225 | Glu-richAdd BLAST | 82 | |
Compositional biasi | 428 – 459 | Gly-richAdd BLAST | 32 |
Domaini
Phylogenomic databases
eggNOGi | KOG0116 Eukaryota ENOG410YV57 LUCA |
GeneTreei | ENSGT00390000011365 |
InParanoidi | P97855 |
KOi | K17265 |
OMAi | TNIPPQR |
OrthoDBi | 1526879at2759 |
TreeFami | TF325464 |
Family and domain databases
CDDi | cd00780 NTF2, 1 hit cd12463 RRM_G3BP1, 1 hit |
Gene3Di | 3.30.70.330, 1 hit |
InterProi | View protein in InterPro IPR034375 G3BP1 IPR034374 G3BP1_RRM IPR002075 NTF2 IPR032710 NTF2-like_dom_sf IPR018222 Nuclear_transport_factor_2_euk IPR012677 Nucleotide-bd_a/b_plait_sf IPR039539 Ras_GTPase_bind_prot IPR035979 RBD_domain_sf IPR000504 RRM_dom |
PANTHERi | PTHR10693 PTHR10693, 1 hit PTHR10693:SF21 PTHR10693:SF21, 1 hit |
Pfami | View protein in Pfam PF02136 NTF2, 1 hit PF00076 RRM_1, 1 hit |
SMARTi | View protein in SMART SM00360 RRM, 1 hit |
SUPFAMi | SSF54427 SSF54427, 1 hit SSF54928 SSF54928, 1 hit |
PROSITEi | View protein in PROSITE PS50177 NTF2_DOMAIN, 1 hit PS50102 RRM, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MVMEKPSPLL VGREFVRQYY TLLNQAPDML HRFYGKNSSY AHGGLDSNGK
60 70 80 90 100
PADAVYGQKE IHRKVMSQNF TNCHTKIRHV DAHATLNDGV VVQVMGLLSN
110 120 130 140 150
NNQALRRFMQ TFVLAPEGSV ANKFYVHNDI FRYQDEVFGG FVTEPQEESE
160 170 180 190 200
EEVEEPEERQ QTPEVVPDDS GTFYDQTVSN DLEEHLEEPV VEPEPEPEPE
210 220 230 240 250
PEPEPVSDIQ EDKPEAALEE AAPDDVQKST SPAPADVAPA QEDLRTFSWA
260 270 280 290 300
SVTSKNLPPS GAVPVTGTPP HVVKVPASQP RPESKPDSQI PPQRPQRDQR
310 320 330 340 350
VREQRINIPP QRGPRPIREA GEPGDVEPRR MVRHPDSHQL FIGNLPHEVD
360 370 380 390 400
KSELKDFFQN FGNVVELRIN SGGKLPNFGF VVFDDSEPVQ KVLSNRPIMF
410 420 430 440 450
RGAVRLNVEE KKTRAAREGD RRDNRLRGPG GPRGGPSGGM RGPPRGGMVQ
460
KPGFGVGRGI TTPRQ
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB001927 mRNA Translation: BAA19469.1 BC021156 mRNA Translation: AAH21156.1 |
CCDSi | CCDS24714.1 |
RefSeqi | NP_038744.1, NM_013716.2 |
Genome annotation databases
Ensembli | ENSMUST00000018727; ENSMUSP00000018727; ENSMUSG00000018583 |
GeneIDi | 27041 |
KEGGi | mmu:27041 |
UCSCi | uc007izl.1 mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB001927 mRNA Translation: BAA19469.1 BC021156 mRNA Translation: AAH21156.1 |
CCDSi | CCDS24714.1 |
RefSeqi | NP_038744.1, NM_013716.2 |
3D structure databases
SMRi | P97855 |
ModBasei | Search... |
Protein-protein interaction databases
BioGridi | 205102, 10 interactors |
IntActi | P97855, 11 interactors |
MINTi | P97855 |
STRINGi | 10090.ENSMUSP00000018727 |
PTM databases
iPTMneti | P97855 |
PhosphoSitePlusi | P97855 |
SwissPalmi | P97855 |
Proteomic databases
EPDi | P97855 |
jPOSTi | P97855 |
PaxDbi | P97855 |
PeptideAtlasi | P97855 |
PRIDEi | P97855 |
Genome annotation databases
Ensembli | ENSMUST00000018727; ENSMUSP00000018727; ENSMUSG00000018583 |
GeneIDi | 27041 |
KEGGi | mmu:27041 |
UCSCi | uc007izl.1 mouse |
Organism-specific databases
CTDi | 10146 |
MGIi | MGI:1351465 G3bp1 |
Phylogenomic databases
eggNOGi | KOG0116 Eukaryota ENOG410YV57 LUCA |
GeneTreei | ENSGT00390000011365 |
InParanoidi | P97855 |
KOi | K17265 |
OMAi | TNIPPQR |
OrthoDBi | 1526879at2759 |
TreeFami | TF325464 |
Miscellaneous databases
ChiTaRSi | G3bp1 mouse |
PROi | PR:P97855 |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000018583 Expressed in 317 organ(s), highest expression level in trachea |
Genevisiblei | P97855 MM |
Family and domain databases
CDDi | cd00780 NTF2, 1 hit cd12463 RRM_G3BP1, 1 hit |
Gene3Di | 3.30.70.330, 1 hit |
InterProi | View protein in InterPro IPR034375 G3BP1 IPR034374 G3BP1_RRM IPR002075 NTF2 IPR032710 NTF2-like_dom_sf IPR018222 Nuclear_transport_factor_2_euk IPR012677 Nucleotide-bd_a/b_plait_sf IPR039539 Ras_GTPase_bind_prot IPR035979 RBD_domain_sf IPR000504 RRM_dom |
PANTHERi | PTHR10693 PTHR10693, 1 hit PTHR10693:SF21 PTHR10693:SF21, 1 hit |
Pfami | View protein in Pfam PF02136 NTF2, 1 hit PF00076 RRM_1, 1 hit |
SMARTi | View protein in SMART SM00360 RRM, 1 hit |
SUPFAMi | SSF54427 SSF54427, 1 hit SSF54928 SSF54928, 1 hit |
PROSITEi | View protein in PROSITE PS50177 NTF2_DOMAIN, 1 hit PS50102 RRM, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | G3BP1_MOUSE | |
Accessioni | P97855Primary (citable) accession number: P97855 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 11, 2001 |
Last sequence update: | May 1, 1997 | |
Last modified: | November 13, 2019 | |
This is version 169 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Complete proteome, Direct protein sequencing, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot