UniProtKB - P97831 (TWST2_RAT)
Twist-related protein 2
Twist2
Functioni
Binds to the E-box consensus sequence 5'-CANNTG-3' as a heterodimer and inhibits transcriptional activation by MYOD1, MYOG, MEF2A and MEF2C. Also represses expression of pro-inflammatory cytokines such as TNFA and IL1B. Involved in postnatal glycogen storage and energy metabolism (By similarity).
Inhibits the premature or ectopic differentiation of preosteoblast cells during osteogenesis, possibly by changing the internal signal transduction response of osteoblasts to external growth factors (By similarity).
By similarityGO - Molecular functioni
- chromatin binding Source: RGD
- DNA-binding transcription factor activity Source: RGD
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: GO_Central
- protein dimerization activity Source: InterPro
- protein domain specific binding Source: RGD
- RNA polymerase II transcription regulatory region sequence-specific DNA binding Source: GO_Central
GO - Biological processi
- cornea development in camera-type eye Source: RGD
- developmental process Source: GO_Central
- embryonic cranial skeleton morphogenesis Source: RGD
- face morphogenesis Source: RGD
- negative regulation of apoptotic process Source: RGD
- negative regulation of cell population proliferation Source: RGD
- negative regulation of DNA binding Source: RGD
- negative regulation of molecular function Source: RGD
- negative regulation of myeloid cell differentiation Source: RGD
- negative regulation of osteoblast differentiation Source: UniProtKB
- negative regulation of transcription, DNA-templated Source: UniProtKB
- negative regulation of transcription by RNA polymerase II Source: RGD
- negative regulation of tumor necrosis factor production Source: RGD
- osteoblast differentiation Source: RGD
- positive regulation of cell migration Source: RGD
- positive regulation of keratinocyte proliferation Source: RGD
- regulation of transcription by RNA polymerase II Source: RGD
Keywordsi
Molecular function | Developmental protein, DNA-binding, Repressor |
Biological process | Differentiation, Transcription, Transcription regulation |
Names & Taxonomyi
Protein namesi | Recommended name: Twist-related protein 2 |
Gene namesi | Name:Twist2 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 621286, Twist2 |
Subcellular locationi
Nucleus
- nucleolus Source: Ensembl
- nucleoplasm Source: Ensembl
- nucleus Source: UniProtKB
Other locations
- cytoplasm Source: UniProtKB
- transcription regulator complex Source: RGD
Keywords - Cellular componenti
Cytoplasm, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000127491 | 1 – 160 | Twist-related protein 2Add BLAST | 160 |
Proteomic databases
PaxDbi | P97831 |
PTM databases
PhosphoSitePlusi | P97831 |
Expressioni
Gene expression databases
Bgeei | ENSRNOG00000020355, Expressed in esophagus and 21 other tissues |
Genevisiblei | P97831, RN |
Interactioni
Subunit structurei
Efficient DNA binding requires dimerization with another bHLH protein.
Forms a heterodimer with TCF3/E12.
Also interacts with MEF2C (By similarity).
By similarityGO - Molecular functioni
- protein dimerization activity Source: InterPro
- protein domain specific binding Source: RGD
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000027597 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 66 – 117 | bHLHPROSITE-ProRule annotationAdd BLAST | 52 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 63 | DisorderedSequence analysisAdd BLAST | 63 |
Phylogenomic databases
eggNOGi | KOG4447, Eukaryota |
GeneTreei | ENSGT00940000161996 |
HOGENOMi | CLU_112073_0_1_1 |
InParanoidi | P97831 |
OMAi | SNQSYEE |
OrthoDBi | 1595261at2759 |
PhylomeDBi | P97831 |
TreeFami | TF315153 |
Family and domain databases
Gene3Di | 4.10.280.10, 1 hit |
InterProi | View protein in InterPro IPR011598, bHLH_dom IPR036638, HLH_DNA-bd_sf IPR015789, Twist-related |
PANTHERi | PTHR23349:SF70, PTHR23349:SF70, 1 hit |
Pfami | View protein in Pfam PF00010, HLH, 1 hit |
SMARTi | View protein in SMART SM00353, HLH, 1 hit |
SUPFAMi | SSF47459, SSF47459, 1 hit |
PROSITEi | View protein in PROSITE PS50888, BHLH, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MEEGSSSPVS PVDSLGTSEE ELERQPKRFG RKRRYSKKSS EDGSPTPGKR
60 70 80 90 100
GKKGSPSAQS FEELQSQRIL ANVRERQRTQ SLNEAFAALR KIIPTLPSDK
110 120 130 140 150
LSKIQTLKLA ARYIDFLYQV LQSDEMDNKM TSCSYVAHER LSYAFSVWRM
160
EGAWSMSASH
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Y08139 mRNA Translation: CAA69333.1 |
RefSeqi | NP_067723.1, NM_021691.2 |
Genome annotation databases
Ensembli | ENSRNOT00000027597; ENSRNOP00000027597; ENSRNOG00000020355 |
GeneIDi | 59327 |
KEGGi | rno:59327 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Y08139 mRNA Translation: CAA69333.1 |
RefSeqi | NP_067723.1, NM_021691.2 |
3D structure databases
AlphaFoldDBi | P97831 |
SMRi | P97831 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000027597 |
PTM databases
PhosphoSitePlusi | P97831 |
Proteomic databases
PaxDbi | P97831 |
Genome annotation databases
Ensembli | ENSRNOT00000027597; ENSRNOP00000027597; ENSRNOG00000020355 |
GeneIDi | 59327 |
KEGGi | rno:59327 |
Organism-specific databases
CTDi | 117581 |
RGDi | 621286, Twist2 |
Phylogenomic databases
eggNOGi | KOG4447, Eukaryota |
GeneTreei | ENSGT00940000161996 |
HOGENOMi | CLU_112073_0_1_1 |
InParanoidi | P97831 |
OMAi | SNQSYEE |
OrthoDBi | 1595261at2759 |
PhylomeDBi | P97831 |
TreeFami | TF315153 |
Miscellaneous databases
PROi | PR:P97831 |
Gene expression databases
Bgeei | ENSRNOG00000020355, Expressed in esophagus and 21 other tissues |
Genevisiblei | P97831, RN |
Family and domain databases
Gene3Di | 4.10.280.10, 1 hit |
InterProi | View protein in InterPro IPR011598, bHLH_dom IPR036638, HLH_DNA-bd_sf IPR015789, Twist-related |
PANTHERi | PTHR23349:SF70, PTHR23349:SF70, 1 hit |
Pfami | View protein in Pfam PF00010, HLH, 1 hit |
SMARTi | View protein in SMART SM00353, HLH, 1 hit |
SUPFAMi | SSF47459, SSF47459, 1 hit |
PROSITEi | View protein in PROSITE PS50888, BHLH, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | TWST2_RAT | |
Accessioni | P97831Primary (citable) accession number: P97831 Secondary accession number(s): Q60981 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 11, 2003 |
Last sequence update: | May 1, 1999 | |
Last modified: | May 25, 2022 | |
This is version 150 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |