UniProtKB - P97829 (CD47_RAT)
Protein
Leukocyte surface antigen CD47
Gene
Cd47
Organism
Rattus norvegicus (Rat)
Status
Functioni
Has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. Receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation. May play a role in membrane transport and/or integrin dependent signal transduction. May prevent premature elimination of red blood cells. May be involved in membrane permeability changes induced following virus infection (By similarity). Plays an important role in memory formation and synaptic plasticity in the hippocampus.By similarity1 Publication
GO - Molecular functioni
- cell-cell adhesion mediator activity Source: RGD
- protein binding involved in heterotypic cell-cell adhesion Source: ARUK-UCL
- thrombospondin receptor activity Source: RGD
GO - Biological processi
- cell migration Source: RGD
- cellular response to interferon-gamma Source: ARUK-UCL
- cellular response to interleukin-1 Source: ARUK-UCL
- cellular response to interleukin-12 Source: RGD
- integrin-mediated signaling pathway Source: UniProtKB-KW
- monocyte extravasation Source: ARUK-UCL
- negative regulation of Fc-gamma receptor signaling pathway involved in phagocytosis Source: ARUK-UCL
- opsonization Source: RGD
- positive regulation of cell-cell adhesion Source: RGD
- positive regulation of cell population proliferation Source: RGD
- positive regulation of inflammatory response Source: RGD
- positive regulation of phagocytosis Source: RGD
- positive regulation of stress fiber assembly Source: ARUK-UCL
- positive regulation of T cell activation Source: RGD
- regulation of interferon-gamma production Source: RGD
- regulation of interleukin-10 production Source: RGD
- regulation of interleukin-12 production Source: RGD
- regulation of interleukin-6 production Source: RGD
- regulation of nitric oxide biosynthetic process Source: RGD
- regulation of tumor necrosis factor production Source: RGD
- response to bacterium Source: RGD
Keywordsi
Molecular function | Integrin |
Biological process | Cell adhesion |
Enzyme and pathway databases
Reactomei | R-RNO-202733, Cell surface interactions at the vascular wall R-RNO-216083, Integrin cell surface interactions R-RNO-391160, Signal regulatory protein family interactions R-RNO-6798695, Neutrophil degranulation |
Names & Taxonomyi
Protein namesi | Recommended name: Leukocyte surface antigen CD47Alternative name(s): Integrin-associated protein Short name: IAP CD_antigen: CD47 |
Gene namesi | Name:Cd47 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 2308, Cd47 |
Subcellular locationi
Plasma membrane
- Cell membrane By similarity; Multi-pass membrane protein By similarity
Extracellular region or secreted
- extracellular exosome Source: RGD
Plasma Membrane
- integral component of plasma membrane Source: RGD
Other locations
- cell surface Source: ARUK-UCL
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 19 – 140 | ExtracellularSequence analysisAdd BLAST | 122 | |
Transmembranei | 141 – 161 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 162 – 173 | CytoplasmicSequence analysisAdd BLAST | 12 | |
Transmembranei | 174 – 194 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 195 – 206 | ExtracellularSequence analysisAdd BLAST | 12 | |
Transmembranei | 207 – 227 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 228 – 238 | CytoplasmicSequence analysisAdd BLAST | 11 | |
Transmembranei | 239 – 259 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 260 – 266 | ExtracellularSequence analysis | 7 | |
Transmembranei | 267 – 287 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 288 – 303 | CytoplasmicSequence analysisAdd BLAST | 16 |
Keywords - Cellular componenti
Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 18 | Sequence analysisAdd BLAST | 18 | |
ChainiPRO_0000042209 | 19 – 303 | Leukocyte surface antigen CD47Add BLAST | 285 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length | |
---|---|---|---|---|---|
Modified residuei | 19 | Pyrrolidone carboxylic acidBy similarityCurated | 1 | ||
Disulfide bondi | 33 ↔ 261 | PROSITE-ProRule annotation | |||
Glycosylationi | 34 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | ||
Disulfide bondi | 41 ↔ 112 | PROSITE-ProRule annotation | |||
Glycosylationi | 61 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | ||
Glycosylationi | 73 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | ||
Glycosylationi | 80 | N-linked (GlcNAc...) asparagineCombined sources | 1 | ||
Modified residuei | 87 | PhosphoserineCombined sources | 1 | ||
Modified residuei | 89 | PhosphoserineCombined sources | 1 | ||
Glycosylationi | 109 | N-linked (GlcNAc...) asparagineCombined sources | 1 | ||
Glycosylationi | 204 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | ||
Isoform 2 (identifier: P97829-2) | |||||
Modified residuei | 311 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
Disulfide bond, Glycoprotein, Phosphoprotein, Pyrrolidone carboxylic acidProteomic databases
PaxDbi | P97829 |
PRIDEi | P97829 |
PTM databases
GlyGeni | P97829, 6 sites, 23 N-linked glycans (3 sites) |
iPTMneti | P97829 |
PhosphoSitePlusi | P97829 |
SwissPalmi | P97829 |
UniCarbKBi | P97829 |
Expressioni
Tissue specificityi
Expressed in hippocampus.1 Publication
Inductioni
By ferric nitrilotriacetate, NMDA and amphetamine.2 Publications
Gene expression databases
Bgeei | ENSRNOG00000001964, Expressed in pancreas and 21 other tissues |
ExpressionAtlasi | P97829, baseline and differential |
Genevisiblei | P97829, RN |
Interactioni
Subunit structurei
Monomer.
Interacts with fibrinogen, PTPNS1, SIRPG, THBS1, UBQLN1 and UBQLN2 (By similarity).
By similarityGO - Molecular functioni
- cell-cell adhesion mediator activity Source: RGD
- protein binding involved in heterotypic cell-cell adhesion Source: ARUK-UCL
Protein-protein interaction databases
BioGRIDi | 248018, 2 interactors |
STRINGi | 10116.ENSRNOP00000050938 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 19 – 125 | Ig-like V-typeAdd BLAST | 107 |
Keywords - Domaini
Immunoglobulin domain, Signal, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | ENOG502RYTQ, Eukaryota |
GeneTreei | ENSGT00390000007697 |
InParanoidi | P97829 |
Family and domain databases
CDDi | cd16090, IgV_CD47, 1 hit |
Gene3Di | 2.60.40.10, 1 hit |
InterProi | View protein in InterPro IPR013147, CD47_TM IPR013270, CD47_Vset IPR007110, Ig-like_dom IPR013783, Ig-like_fold IPR037805, IgV_CD47 |
Pfami | View protein in Pfam PF04549, CD47, 1 hit PF08204, V-set_CD47, 1 hit |
PROSITEi | View protein in PROSITE PS50835, IG_LIKE, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
Isoform 1 (identifier: P97829-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MWPLAAALLL GSCCCGSAQL LLSKVKSVEF TSCNDTVVIP CKVLNVEAQS
60 70 80 90 100
TDEMFVKWKL NKSYIFIYDG NKNSTTREQN FTSAKISVSD LLKGIASLTM
110 120 130 140 150
DTHEAVVGNY TCEVTELSRE GKTVIELKNR PVSWFSTNEK ILIVIFPILA
160 170 180 190 200
ILLFWGKFGI LTLKYKSSHT NKRIILLLVA GLALTLIVVV GAILFIPGEK
210 220 230 240 250
PVKNASGLGL IVISTGILIL LQYNVFMTAF GMTSFTIAIL ITQVLGYVLA
260 270 280 290 300
VVGMCLCIMA CEPVHGPLLI SGLGIIALAE LLGLVYMKFV ASNQRTIQPP
RNN
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A0G2JTH4 | A0A0G2JTH4_RAT | Integrin-associated protein | Cd47 | 359 | Annotation score: |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_015794 | 8 – 11 | LLLG → R in isoform 2. 1 Publication | 4 | |
Alternative sequenceiVSP_015795 | 302 – 303 | NN → KAVEEPLNAFKESKGMMNDE in isoform 2. 1 Publication | 2 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D87659 mRNA Translation: BAA13420.1 AF017437 mRNA Translation: AAB70273.1 BC085740 mRNA Translation: AAH85740.1 |
RefSeqi | NP_062068.1, NM_019195.2 [P97829-1] |
Genome annotation databases
Ensembli | ENSRNOT00000050142; ENSRNOP00000050938; ENSRNOG00000001964 [P97829-1] ENSRNOT00000078434; ENSRNOP00000071430; ENSRNOG00000001964 [P97829-1] |
GeneIDi | 29364 |
KEGGi | rno:29364 |
UCSCi | RGD:2308, rat [P97829-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D87659 mRNA Translation: BAA13420.1 AF017437 mRNA Translation: AAB70273.1 BC085740 mRNA Translation: AAH85740.1 |
RefSeqi | NP_062068.1, NM_019195.2 [P97829-1] |
3D structure databases
SMRi | P97829 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 248018, 2 interactors |
STRINGi | 10116.ENSRNOP00000050938 |
PTM databases
GlyGeni | P97829, 6 sites, 23 N-linked glycans (3 sites) |
iPTMneti | P97829 |
PhosphoSitePlusi | P97829 |
SwissPalmi | P97829 |
UniCarbKBi | P97829 |
Proteomic databases
PaxDbi | P97829 |
PRIDEi | P97829 |
Genome annotation databases
Ensembli | ENSRNOT00000050142; ENSRNOP00000050938; ENSRNOG00000001964 [P97829-1] ENSRNOT00000078434; ENSRNOP00000071430; ENSRNOG00000001964 [P97829-1] |
GeneIDi | 29364 |
KEGGi | rno:29364 |
UCSCi | RGD:2308, rat [P97829-1] |
Organism-specific databases
CTDi | 961 |
RGDi | 2308, Cd47 |
Phylogenomic databases
eggNOGi | ENOG502RYTQ, Eukaryota |
GeneTreei | ENSGT00390000007697 |
InParanoidi | P97829 |
Enzyme and pathway databases
Reactomei | R-RNO-202733, Cell surface interactions at the vascular wall R-RNO-216083, Integrin cell surface interactions R-RNO-391160, Signal regulatory protein family interactions R-RNO-6798695, Neutrophil degranulation |
Miscellaneous databases
PROi | PR:P97829 |
Gene expression databases
Bgeei | ENSRNOG00000001964, Expressed in pancreas and 21 other tissues |
ExpressionAtlasi | P97829, baseline and differential |
Genevisiblei | P97829, RN |
Family and domain databases
CDDi | cd16090, IgV_CD47, 1 hit |
Gene3Di | 2.60.40.10, 1 hit |
InterProi | View protein in InterPro IPR013147, CD47_TM IPR013270, CD47_Vset IPR007110, Ig-like_dom IPR013783, Ig-like_fold IPR037805, IgV_CD47 |
Pfami | View protein in Pfam PF04549, CD47, 1 hit PF08204, V-set_CD47, 1 hit |
PROSITEi | View protein in PROSITE PS50835, IG_LIKE, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CD47_RAT | |
Accessioni | P97829Primary (citable) accession number: P97829 Secondary accession number(s): O35294 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 27, 2005 |
Last sequence update: | May 1, 1997 | |
Last modified: | December 2, 2020 | |
This is version 129 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |