UniProtKB - P97822 (AN32E_MOUSE)
Protein
Acidic leucine-rich nuclear phosphoprotein 32 family member E
Gene
Anp32e
Organism
Mus musculus (Mouse)
Status
Functioni
Histone chaperone that specifically mediates the genome-wide removal of histone H2A.Z/H2AZ1 from the nucleosome: removes H2A.Z/H2AZ1 from its normal sites of deposition, especially from enhancer and insulator regions. Not involved in deposition of H2A.Z/H2AZ1 in the nucleosome. May stabilize the evicted H2A.Z/H2AZ1-H2B dimer, thus shifting the equilibrium towards dissociation and the off-chromatin state (PubMed:24463511). Inhibits activity of protein phosphatase 2A (PP2A). Does not inhibit protein phosphatase 1. May play a role in cerebellar development and synaptogenesis.4 Publications
GO - Molecular functioni
- histone binding Source: UniProtKB
- phosphatase inhibitor activity Source: MGI
GO - Biological processi
- histone exchange Source: UniProtKB
- regulation of apoptotic process Source: GO_Central
Keywordsi
Molecular function | Chaperone, Chromatin regulator |
Names & Taxonomyi
Protein namesi | Recommended name: Acidic leucine-rich nuclear phosphoprotein 32 family member EAlternative name(s): Cerebellar postnatal development protein 1 LANP-like protein Short name: LANP-L |
Gene namesi | Name:Anp32e Synonyms:Cpd1 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1913721, Anp32e |
Subcellular locationi
Nucleus
- nucleus Source: MGI
- Swr1 complex Source: UniProtKB
Other locations
- cytoplasm Source: MGI
- cytoplasmic vesicle Source: MGI
Keywords - Cellular componenti
Cytoplasm, NucleusPathology & Biotechi
Disruption phenotypei
No visible phenotype. Mice are viable and fertile (PubMed:20844742, PubMed:21049064, PubMed:23675506). They display a subtle neurological clasping phenotype and mild motor deficits (PubMed:20844742). Motor defects were not confirmed by a subsequent analysis (PubMed:23675506). Deletion in embryonic fibroblasts results in the appearance of a significant number of new H2A.Z/H2AZ1 around the transcription start site as well as at other chromatin regions (PubMed:24463511).4 Publications
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000137600 | 1 – 260 | Acidic leucine-rich nuclear phosphoprotein 32 family member EAdd BLAST | 260 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionineBy similarity | 1 | |
Cross-linki | 68 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity |
Post-translational modificationi
Phosphorylated. The phosphorylation is nuclear localization signal (NLS)-dependent (By similarity).By similarity
Keywords - PTMi
Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | P97822 |
jPOSTi | P97822 |
MaxQBi | P97822 |
PaxDbi | P97822 |
PeptideAtlasi | P97822 |
PRIDEi | P97822 |
PTM databases
iPTMneti | P97822 |
PhosphoSitePlusi | P97822 |
SwissPalmi | P97822 |
Expressioni
Tissue specificityi
Expressed at highest levels in cerebellum and spleen. In the cerebellum, expressed mainly in granule cells and, to a lesser extent, in Purkinje cells.1 Publication
Developmental stagei
Low levels are found at postnatal day 4. Levels increase from postnatal day 7 to postnatal day 17. Levels decrease and remain low in the adult.1 Publication
Gene expression databases
Bgeei | ENSMUSG00000015749, Expressed in embryonic post-anal tail and 312 other tissues |
Genevisiblei | P97822, MM |
Interactioni
Subunit structurei
Component of a SWR1-like complex, composed of EP400, KAT5/TIP60, TRRAP, BRD8, RUVBL1, RUVBL2, ING3 and ANP32E; the complex does not contain SRCAP.
Interacts with H2A.Z/H2AZ1 (By similarity).
Interacts with the importin alpha KPNA1 and KPNA2.
By similarity1 PublicationGO - Molecular functioni
- histone binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 211498, 1 interactor |
IntActi | P97822, 4 interactors |
MINTi | P97822 |
STRINGi | 10090.ENSMUSP00000128483 |
Miscellaneous databases
RNActi | P97822, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 18 – 38 | LRR 1Add BLAST | 21 | |
Repeati | 43 – 64 | LRR 2Add BLAST | 22 | |
Repeati | 65 – 87 | LRR 3Add BLAST | 23 | |
Repeati | 89 – 110 | LRR 4Add BLAST | 22 | |
Domaini | 123 – 161 | LRRCTAdd BLAST | 39 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 207 – 260 | ZID domainBy similarityAdd BLAST | 54 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 151 – 260 | Asp/Glu-rich (highly acidic)Add BLAST | 110 |
Domaini
The H2A.Z-interacting domain (ZID) mediates a direct interaction with H2A.Z/H2AZ1.By similarity
Sequence similaritiesi
Belongs to the ANP32 family.Curated
Keywords - Domaini
Leucine-rich repeat, RepeatPhylogenomic databases
eggNOGi | KOG2739, Eukaryota |
GeneTreei | ENSGT00950000182907 |
InParanoidi | P97822 |
OMAi | HFASVFG |
OrthoDBi | 1622194at2759 |
PhylomeDBi | P97822 |
TreeFami | TF317206 |
Family and domain databases
Gene3Di | 3.80.10.10, 1 hit |
InterProi | View protein in InterPro IPR001611, Leu-rich_rpt IPR032675, LRR_dom_sf |
PROSITEi | View protein in PROSITE PS51450, LRR, 4 hits |
s (3+)i Sequence
Sequence statusi: Complete.
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 3 described isoforms and 6 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: P97822-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MEMKKKINME LKNRAPEEVT ELVLDNCLCV NGEIEGLNDT FKELEFLSMA
60 70 80 90 100
NVELSSLARL PSLNKLRKLE LSDNIISGGL EVLAEKCPNL TYLNLSGNKI
110 120 130 140 150
KDLSTVEALQ NLKNLKSLDL FNCEITNLED YRESIFELLQ QITYLDGFDQ
160 170 180 190 200
EDNEAPDSEE EDDDDEDGDE DEEDEDEDEA GPPEGYEEEE DDDEDEAGSE
210 220 230 240 250
VGEGEEEVGL SYLMKDEIQD EEDDDDYVDE GEEEEEEEEE GLRGEKRKRD
260
AEDDGEEDDD
Computationally mapped potential isoform sequencesi
There are 6 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketE9PZF5 | E9PZF5_MOUSE | Acidic leucine-rich nuclear phospho... | Anp32e | 171 | Annotation score: | ||
E9Q0X5 | E9Q0X5_MOUSE | Acidic leucine-rich nuclear phospho... | Anp32e | 219 | Annotation score: | ||
E9Q2G1 | E9Q2G1_MOUSE | Acidic leucine-rich nuclear phospho... | Anp32e | 109 | Annotation score: | ||
A0A0G2JEK9 | A0A0G2JEK9_MOUSE | Acidic leucine-rich nuclear phospho... | Anp32e | 71 | Annotation score: | ||
E9PVI5 | E9PVI5_MOUSE | Acidic leucine-rich nuclear phospho... | Anp32e | 46 | Annotation score: | ||
E9Q5H2 | E9Q5H2_MOUSE | Acidic leucine-rich nuclear phospho... | Anp32e | 107 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 38 | N → D in BAE35090 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 98 | N → D in BAB28449 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 199 | S → T in BAC33858 (PubMed:16141072).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_059599 | 19 – 141 | Missing in isoform 3. CuratedAdd BLAST | 123 | |
Alternative sequenceiVSP_007373 | 208 – 219 | Missing in isoform 2. 1 PublicationAdd BLAST | 12 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U89345 mRNA Translation: AAB49462.2 AB037685 mRNA Translation: BAB03507.1 AC092855 Genomic DNA No translation available. AK012759 mRNA Translation: BAB28449.1 AK049647 mRNA Translation: BAC33858.1 AK076049 mRNA Translation: BAC36147.1 AK088401 mRNA Translation: BAC40331.1 AK147888 mRNA Translation: BAE28205.1 AK159446 mRNA Translation: BAE35090.1 AK168380 mRNA Translation: BAE40309.1 BC005690 mRNA Translation: AAH05690.1 BC080684 mRNA Translation: AAH80684.1 |
CCDSi | CCDS17626.1 [P97822-1] CCDS57238.1 [P97822-2] CCDS57239.1 [P97822-3] |
RefSeqi | NP_001240686.1, NM_001253757.1 [P97822-2] NP_001240687.1, NM_001253758.1 [P97822-3] NP_075699.3, NM_023210.4 [P97822-1] |
Genome annotation databases
Ensembli | ENSMUST00000015893; ENSMUSP00000015893; ENSMUSG00000015749 [P97822-2] ENSMUST00000165307; ENSMUSP00000128483; ENSMUSG00000015749 [P97822-1] ENSMUST00000171368; ENSMUSP00000130599; ENSMUSG00000015749 [P97822-3] |
GeneIDi | 66471 |
KEGGi | mmu:66471 |
UCSCi | uc008qlu.2, mouse [P97822-1] uc008qlv.2, mouse [P97822-2] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U89345 mRNA Translation: AAB49462.2 AB037685 mRNA Translation: BAB03507.1 AC092855 Genomic DNA No translation available. AK012759 mRNA Translation: BAB28449.1 AK049647 mRNA Translation: BAC33858.1 AK076049 mRNA Translation: BAC36147.1 AK088401 mRNA Translation: BAC40331.1 AK147888 mRNA Translation: BAE28205.1 AK159446 mRNA Translation: BAE35090.1 AK168380 mRNA Translation: BAE40309.1 BC005690 mRNA Translation: AAH05690.1 BC080684 mRNA Translation: AAH80684.1 |
CCDSi | CCDS17626.1 [P97822-1] CCDS57238.1 [P97822-2] CCDS57239.1 [P97822-3] |
RefSeqi | NP_001240686.1, NM_001253757.1 [P97822-2] NP_001240687.1, NM_001253758.1 [P97822-3] NP_075699.3, NM_023210.4 [P97822-1] |
3D structure databases
SMRi | P97822 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 211498, 1 interactor |
IntActi | P97822, 4 interactors |
MINTi | P97822 |
STRINGi | 10090.ENSMUSP00000128483 |
PTM databases
iPTMneti | P97822 |
PhosphoSitePlusi | P97822 |
SwissPalmi | P97822 |
Proteomic databases
EPDi | P97822 |
jPOSTi | P97822 |
MaxQBi | P97822 |
PaxDbi | P97822 |
PeptideAtlasi | P97822 |
PRIDEi | P97822 |
Protocols and materials databases
Antibodypediai | 20276, 133 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000015893; ENSMUSP00000015893; ENSMUSG00000015749 [P97822-2] ENSMUST00000165307; ENSMUSP00000128483; ENSMUSG00000015749 [P97822-1] ENSMUST00000171368; ENSMUSP00000130599; ENSMUSG00000015749 [P97822-3] |
GeneIDi | 66471 |
KEGGi | mmu:66471 |
UCSCi | uc008qlu.2, mouse [P97822-1] uc008qlv.2, mouse [P97822-2] |
Organism-specific databases
CTDi | 81611 |
MGIi | MGI:1913721, Anp32e |
Phylogenomic databases
eggNOGi | KOG2739, Eukaryota |
GeneTreei | ENSGT00950000182907 |
InParanoidi | P97822 |
OMAi | HFASVFG |
OrthoDBi | 1622194at2759 |
PhylomeDBi | P97822 |
TreeFami | TF317206 |
Miscellaneous databases
BioGRID-ORCSi | 66471, 2 hits in 16 CRISPR screens |
ChiTaRSi | Anp32e, mouse |
PROi | PR:P97822 |
RNActi | P97822, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000015749, Expressed in embryonic post-anal tail and 312 other tissues |
Genevisiblei | P97822, MM |
Family and domain databases
Gene3Di | 3.80.10.10, 1 hit |
InterProi | View protein in InterPro IPR001611, Leu-rich_rpt IPR032675, LRR_dom_sf |
PROSITEi | View protein in PROSITE PS51450, LRR, 4 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | AN32E_MOUSE | |
Accessioni | P97822Primary (citable) accession number: P97822 Secondary accession number(s): E9Q5H9 Q9CZD2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 9, 2003 |
Last sequence update: | May 1, 2000 | |
Last modified: | December 2, 2020 | |
This is version 163 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families