UniProtKB - P97798 (NEO1_MOUSE)
Protein
Neogenin
Gene
Neo1
Organism
Mus musculus (Mouse)
Status
Functioni
Multi-functional cell surface receptor regulating cell adhesion in many diverse developmental processes, including neural tube and mammary gland formation, myogenesis and angiogenesis. Receptor for members of the BMP, netrin, and repulsive guidance molecule (RGM) families. Netrin-Neogenin interactions result in a chemoattractive axon guidance response and cell-cell adhesion, the interaction between NEO1/Neogenin and RGMa and RGMb induces a chemorepulsive response.1 Publication
GO - Molecular functioni
- BMP receptor binding Source: BHF-UCL
- cadherin binding Source: MGI
- co-receptor binding Source: MGI
- signaling receptor activity Source: MGI
GO - Biological processi
- axon guidance Source: InterPro
- cell adhesion Source: UniProtKB-KW
- iron ion homeostasis Source: MGI
- myoblast fusion Source: MGI
- negative regulation of axon regeneration Source: MGI
- negative regulation of neuron death Source: MGI
- negative regulation of protein secretion Source: MGI
- neuron migration Source: MGI
- positive regulation of BMP signaling pathway Source: MGI
- regulation of axon regeneration Source: MGI
- regulation of neuron migration Source: MGI
- regulation of transcription, DNA-templated Source: MGI
Keywordsi
Biological process | Cell adhesion |
Names & Taxonomyi
Protein namesi | Recommended name: Neogenin |
Gene namesi | Name:Neo1 Synonyms:Ngn |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1097159, Neo1 |
Subcellular locationi
Plasma membrane
Golgi apparatus
- Golgi apparatus Source: MGI
Nucleus
- nucleoplasm Source: MGI
Plasma Membrane
- integral component of plasma membrane Source: InterPro
- plasma membrane Source: MGI
- plasma membrane protein complex Source: MGI
Other locations
- cell surface Source: MGI
- intracellular vesicle Source: MGI
- membrane Source: MGI
- neuronal cell body Source: MGI
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 37 – 1136 | ExtracellularSequence analysisAdd BLAST | 1100 | |
Transmembranei | 1137 – 1157 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 1158 – 1493 | CytoplasmicSequence analysisAdd BLAST | 336 |
Keywords - Cellular componenti
Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 36 | Sequence analysisAdd BLAST | 36 | |
ChainiPRO_0000015044 | 37 – 1493 | NeogeninAdd BLAST | 1457 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 84 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 85 ↔ 140 | PROSITE-ProRule annotation | ||
Disulfide bondi | 184 ↔ 232 | PROSITE-ProRule annotation | ||
Glycosylationi | 221 | N-linked (GlcNAc...) asparagine2 Publications | 1 | |
Disulfide bondi | 281 ↔ 331 | PROSITE-ProRule annotation | ||
Glycosylationi | 337 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 373 ↔ 421 | PROSITE-ProRule annotation | ||
Glycosylationi | 501 | N-linked (GlcNAc...) asparagine2 Publications | 1 | |
Glycosylationi | 520 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 670 | N-linked (GlcNAc...) asparagine1 Publication | 1 | |
Glycosylationi | 746 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 940 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Modified residuei | 1209 | PhosphoserineCombined sources | 1 | |
Modified residuei | 1225 | PhosphoserineCombined sources | 1 | |
Modified residuei | 1229 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 1433 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1436 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 1464 | PhosphoserineCombined sources | 1 | |
Modified residuei | 1466 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1467 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
Disulfide bond, Glycoprotein, PhosphoproteinProteomic databases
jPOSTi | P97798 |
MaxQBi | P97798 |
PaxDbi | P97798 |
PeptideAtlasi | P97798 |
PRIDEi | P97798 |
ProteomicsDBi | 287373 [P97798-1] 287374 [P97798-2] 287375 [P97798-3] 287376 [P97798-4] 287377 [P97798-5] |
PTM databases
GlyConnecti | 2440, 1 N-Linked glycan (1 site) [P97798-4] 2441, 1 N-Linked glycan (1 site) [P97798-5] |
GlyGeni | P97798, 8 sites |
iPTMneti | P97798 |
PhosphoSitePlusi | P97798 |
SwissPalmi | P97798 |
Expressioni
Tissue specificityi
Widely expressed.
Developmental stagei
Expressed ubiquitously throughout the mid to late stages of gestation and in adult tissues. Strong expression is observed in the ventral region of the ventricular zone of the 15.5 dpc mouse neural tube, as well as in the ventricular zones of the mesencephalon and rhombencephalon. Isoform 3 and isoform 4 are expressed at higher level compared to other isoforms between 11.5 dpc and 16.5 dpc.
Interactioni
Subunit structurei
Interacts with BMP2, BMP4, BMP6, and BMP7 (By similarity).
Interacts with RGMA and RGMB.
Interacts with MYO10.
By similarity3 PublicationsBinary interactionsi
Hide detailsP97798
With | #Exp. | IntAct |
---|---|---|
Myo10 [F8VQB6] | 3 | EBI-774991,EBI-6445959 |
RGMB [Q6NW40] from Homo sapiens. | 2 | EBI-774991,EBI-16155464 |
GO - Molecular functioni
- BMP receptor binding Source: BHF-UCL
- cadherin binding Source: MGI
- co-receptor binding Source: MGI
Protein-protein interaction databases
DIPi | DIP-32026N |
IntActi | P97798, 6 interactors |
MINTi | P97798 |
STRINGi | 10090.ENSMUSP00000063656 |
Miscellaneous databases
RNActi | P97798, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
BMRBi | P97798 |
SMRi | P97798 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 63 – 158 | Ig-like C2-type 1Add BLAST | 96 | |
Domaini | 163 – 249 | Ig-like C2-type 2Add BLAST | 87 | |
Domaini | 254 – 347 | Ig-like C2-type 3Add BLAST | 94 | |
Domaini | 352 – 437 | Ig-like C2-type 4Add BLAST | 86 | |
Domaini | 472 – 566 | Fibronectin type-III 1PROSITE-ProRule annotationAdd BLAST | 95 | |
Domaini | 572 – 662 | Fibronectin type-III 2PROSITE-ProRule annotationAdd BLAST | 91 | |
Domaini | 667 – 762 | Fibronectin type-III 3PROSITE-ProRule annotationAdd BLAST | 96 | |
Domaini | 772 – 862 | Fibronectin type-III 4PROSITE-ProRule annotationAdd BLAST | 91 | |
Domaini | 887 – 986 | Fibronectin type-III 5PROSITE-ProRule annotationAdd BLAST | 100 | |
Domaini | 988 – 1085 | Fibronectin type-III 6PROSITE-ProRule annotationAdd BLAST | 98 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 1149 – 1153 | Poly-Val | 5 |
Domaini
The Fibronectin repeats 5 and 6 mediate interaction with RGM family molecules.
Sequence similaritiesi
Keywords - Domaini
Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG4221, Eukaryota |
InParanoidi | P97798 |
PhylomeDBi | P97798 |
Family and domain databases
CDDi | cd00063, FN3, 6 hits |
Gene3Di | 2.60.40.10, 10 hits |
InterProi | View protein in InterPro IPR003961, FN3_dom IPR036116, FN3_sf IPR007110, Ig-like_dom IPR036179, Ig-like_dom_sf IPR013783, Ig-like_fold IPR013098, Ig_I-set IPR003599, Ig_sub IPR003598, Ig_sub2 IPR033024, Neogenin IPR010560, Neogenin_C |
PANTHERi | PTHR44170:SF14, PTHR44170:SF14, 1 hit |
Pfami | View protein in Pfam PF00041, fn3, 6 hits PF07679, I-set, 3 hits PF13895, Ig_2, 1 hit PF06583, Neogenin_C, 1 hit |
SMARTi | View protein in SMART SM00060, FN3, 6 hits SM00409, IG, 4 hits SM00408, IGc2, 4 hits |
SUPFAMi | SSF48726, SSF48726, 4 hits SSF49265, SSF49265, 3 hits |
PROSITEi | View protein in PROSITE PS50853, FN3, 6 hits PS50835, IG_LIKE, 4 hits |
s (5+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 5 produced by isoformsialternative splicing. AlignAdd to basketNote: Additional isoforms seem to exist.
This entry has 5 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: P97798-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAAEREAGRL LCTSSSRRCC PPPPLLLLLP LLLLLGRPAS GAAATKSGPR
60 70 80 90 100
RQSQGASVRT FTPFYFLVEP VDTLSVRGSS VILNCSAYSE PSPNIEWKKD
110 120 130 140 150
GTFLNLESDD RRQLLPDGSL FISNVVHSKH NKPDEGFYQC VATVDNLGTI
160 170 180 190 200
VSRTAKLTVA GLPRFTSQPE PSSVYVGNSA ILNCEVNADL VPFVRWEQNR
210 220 230 240 250
QPLLLDDRIV KLPSGTLVIS NATEGDGGLY RCIVESGGPP KFSDEAELKV
260 270 280 290 300
LQDPEEIVDL VFLMRPSSMM KVTGQSAVLP CVVSGLPAPV VRWMKNEEVL
310 320 330 340 350
DTESSGRLVL LAGGCLEISD VTEDDAGTYF CIADNGNKTV EAQAELTVQV
360 370 380 390 400
PPGFLKQPAN IYAHESMDIV FECEVTGKPT PTVKWVKNGD VVIPSDNFKI
410 420 430 440 450
VKEHNLQVLG LVKSDEGFYQ CIAENDVGNA QAGAQLIILE HDVAIPTLPP
460 470 480 490 500
TSLTSATTDH LAPATTGPLP SAPRDVVASL VSTRFIKLTW RTPASDPHGD
510 520 530 540 550
NLTYSVFYTK EGVDRERVEN TSQPGEMQVT IQNLMPATVY IFKVMAQNKH
560 570 580 590 600
GSGESSAPLR VETQPEVQLP GPAPNIRAYA TSPTSITVTW ETPLSGNGEI
610 620 630 640 650
QNYKLYYMEK GTDKEQDIDV SSHSYTINGL KKYTEYSFRV VAYNKHGPGV
660 670 680 690 700
STQDVAVRTL SDVPSAAPQN LSLEVRNSKS IVIHWQPPSS TTQNGQITGY
710 720 730 740 750
KIRYRKASRK SDVTETLVTG TQLSQLIEGL DRGTEYNFRV AALTVNGTGP
760 770 780 790 800
ATDWLSAETF ESDLDETRVP EVPSSLHVRP LVTSIVVSWT PPENQNIVVR
810 820 830 840 850
GYAIGYGIGS PHAQTIKVDY KQRYYTIENL DPSSHYVITL KAFNNVGEGI
860 870 880 890 900
PLYESAVTRP HTDTSEVDLF VINAPYTPVP DPTPMMPPVG VQASILSHDT
910 920 930 940 950
IRITWADNSL PKHQKITDSR YYTVRWKTNI PANTKYKNAN ATTLSYLVTG
960 970 980 990 1000
LKPNTLYEFS VMVTKGRRSS TWSMTAHGAT FELVPTSPPK DVTVVSKEGK
1010 1020 1030 1040 1050
PRTIIVNWQP PSEANGKITG YIIYYSTDVN AEIHDWVIEP VVGNRLTHQI
1060 1070 1080 1090 1100
QELTLDTPYY FKIQARNSKG MGPMSEAVQF RTPKADSSDK MPNDQALGSA
1110 1120 1130 1140 1150
GKGSRLPDLG SDYKPPMSGS NSPHGSPTSP LDSNMLLVII VSVGVITIVV
1160 1170 1180 1190 1200
VVVIAVFCTR RTTSHQKKKR AACKSVNGSH KYKGNCKDVK PPDLWIHHER
1210 1220 1230 1240 1250
LELKPIDKSP DPNPVMTDTP IPRNSQDITP VDNSMDSNIH QRRNSYRGHE
1260 1270 1280 1290 1300
SEDSMSTLAG RRGMRPKMMM PFDSQPPQPV ISAHPIHSLD NPHHHFHSSS
1310 1320 1330 1340 1350
LASPARSHLY HPSSPWPIGT SMSLSDRANS TESVRNTPST DTMPASSSQT
1360 1370 1380 1390 1400
CCTDHQDPEG ATSSSYLASS QEEDSGQSLP TAHVRPSHPL KSFAVPAIPP
1410 1420 1430 1440 1450
PGPPLYDPAL PSTPLLSQQA LEPSTFHSVK TASIGTLGRS RPPMPVVVPS
1460 1470 1480 1490
APEVQETTRM LEDSESSYEP DELTKEMAHL EGLMKDLNAI TTA
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketE9QK04 | E9QK04_MOUSE | Neogenin | Neo1 | 1,492 | Annotation score: | ||
Q7TQG5 | Q7TQG5_MOUSE | Neogenin | Neo1 | 1,465 | Annotation score: | ||
A0A1L1SRD6 | A0A1L1SRD6_MOUSE | Neogenin | Neo1 | 405 | Annotation score: |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_002594 | 442 – 461 | Missing in isoform 2. CuratedAdd BLAST | 20 | |
Alternative sequenceiVSP_002595 | 863 – 878 | Missing in isoform 3. CuratedAdd BLAST | 16 | |
Alternative sequenceiVSP_002596 | 1086 – 1096 | Missing in isoform 4. CuratedAdd BLAST | 11 | |
Alternative sequenceiVSP_002597 | 1279 – 1331 | Missing in isoform 5. CuratedAdd BLAST | 53 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Y09535 mRNA Translation: CAA70727.1 |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Y09535 mRNA Translation: CAA70727.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4BQ6 | X-ray | 2.30 | A/B | 883-1133 | [»] | |
4BQ7 | X-ray | 6.60 | A/B | 883-1133 | [»] | |
4BQ8 | X-ray | 2.80 | A | 883-1083 | [»] | |
4BQ9 | X-ray | 2.91 | A/B | 883-1083 | [»] | |
4BQB | X-ray | 2.70 | A/B/C/D | 883-1133 | [»] | |
4BQC | X-ray | 3.20 | A/B | 883-1133 | [»] | |
4PLN | X-ray | 3.20 | C/D | 765-980 | [»] | |
4UI2 | X-ray | 3.15 | A | 883-1133 | [»] | |
6Z3M | X-ray | 5.50 | E/F/K/L/Q/R | 883-1134 | [»] | |
BMRBi | P97798 | |||||
SMRi | P97798 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
DIPi | DIP-32026N |
IntActi | P97798, 6 interactors |
MINTi | P97798 |
STRINGi | 10090.ENSMUSP00000063656 |
PTM databases
GlyConnecti | 2440, 1 N-Linked glycan (1 site) [P97798-4] 2441, 1 N-Linked glycan (1 site) [P97798-5] |
GlyGeni | P97798, 8 sites |
iPTMneti | P97798 |
PhosphoSitePlusi | P97798 |
SwissPalmi | P97798 |
Proteomic databases
jPOSTi | P97798 |
MaxQBi | P97798 |
PaxDbi | P97798 |
PeptideAtlasi | P97798 |
PRIDEi | P97798 |
ProteomicsDBi | 287373 [P97798-1] 287374 [P97798-2] 287375 [P97798-3] 287376 [P97798-4] 287377 [P97798-5] |
Organism-specific databases
MGIi | MGI:1097159, Neo1 |
Phylogenomic databases
eggNOGi | KOG4221, Eukaryota |
InParanoidi | P97798 |
PhylomeDBi | P97798 |
Miscellaneous databases
ChiTaRSi | Neo1, mouse |
PROi | PR:P97798 |
RNActi | P97798, protein |
SOURCEi | Search... |
Family and domain databases
CDDi | cd00063, FN3, 6 hits |
Gene3Di | 2.60.40.10, 10 hits |
InterProi | View protein in InterPro IPR003961, FN3_dom IPR036116, FN3_sf IPR007110, Ig-like_dom IPR036179, Ig-like_dom_sf IPR013783, Ig-like_fold IPR013098, Ig_I-set IPR003599, Ig_sub IPR003598, Ig_sub2 IPR033024, Neogenin IPR010560, Neogenin_C |
PANTHERi | PTHR44170:SF14, PTHR44170:SF14, 1 hit |
Pfami | View protein in Pfam PF00041, fn3, 6 hits PF07679, I-set, 3 hits PF13895, Ig_2, 1 hit PF06583, Neogenin_C, 1 hit |
SMARTi | View protein in SMART SM00060, FN3, 6 hits SM00409, IG, 4 hits SM00408, IGc2, 4 hits |
SUPFAMi | SSF48726, SSF48726, 4 hits SSF49265, SSF49265, 3 hits |
PROSITEi | View protein in PROSITE PS50853, FN3, 6 hits PS50835, IG_LIKE, 4 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | NEO1_MOUSE | |
Accessioni | P97798Primary (citable) accession number: P97798 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 1, 2000 |
Last sequence update: | May 1, 1997 | |
Last modified: | April 7, 2021 | |
This is version 178 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families