UniProtKB - P97785 (GFRA1_MOUSE)
GDNF family receptor alpha-1
Gfra1
Functioni
Receptor for GDNF. Mediates the GDNF-induced autophosphorylation and activation of the RET receptor (By similarity).
By similarityGO - Molecular functioni
- integrin binding Source: MGI
- neurotrophin receptor activity Source: MGI
- signaling receptor activity Source: GO_Central
GO - Biological processi
- aging Source: Ensembl
- anatomical structure morphogenesis Source: MGI
- cell migration Source: MGI
- kidney development Source: Ensembl
- male gonad development Source: Ensembl
- nervous system development Source: MGI
- neuron differentiation Source: MGI
- neuron projection development Source: MGI
- positive regulation of peptidyl-tyrosine phosphorylation Source: MGI
- transmembrane receptor protein tyrosine kinase signaling pathway Source: MGI
Keywordsi
Molecular function | Receptor |
Enzyme and pathway databases
Reactomei | R-MMU-5673001, RAF/MAP kinase cascade R-MMU-8853659, RET signaling |
Names & Taxonomyi
Protein namesi | Recommended name: GDNF family receptor alpha-1Short name: GDNF receptor alpha-1 Short name: GDNFR-alpha-1 Short name: GFR-alpha-1 Alternative name(s): TGF-beta-related neurotrophic factor receptor 1 |
Gene namesi | Name:Gfra1 Synonyms:Gdnfra, Trnr1 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1100842, Gfra1 |
VEuPathDBi | HostDB:ENSMUSG00000025089 |
Subcellular locationi
Plasma membrane
- Cell membrane By similarity; GPI-anchor By similarity
Golgi apparatus
- trans-Golgi network By similarity
Endosome
- Endosome By similarity
- multivesicular body By similarity
Note: Localizes mainly to the plasma membrane. In the presence of SORL1, shifts to vesicular structures, including trans-Golgi network, endosomes and multivesicular bodies.By similarity
Endosome
- multivesicular body Source: UniProtKB-SubCell
Extracellular region or secreted
- extracellular space Source: MGI
Golgi apparatus
- Golgi apparatus Source: UniProtKB-SubCell
Plasma Membrane
- external side of plasma membrane Source: MGI
- plasma membrane protein complex Source: MGI
Other locations
- anchored component of membrane Source: UniProtKB-KW
- axon Source: MGI
- extrinsic component of membrane Source: MGI
- neuronal cell body Source: MGI
- receptor complex Source: MGI
Keywords - Cellular componenti
Cell membrane, Endosome, Golgi apparatus, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 24 | Sequence analysisAdd BLAST | 24 | |
ChainiPRO_0000010779 | 25 – 430 | GDNF family receptor alpha-1Add BLAST | 406 | |
PropeptideiPRO_0000010780 | 431 – 468 | Removed in mature formSequence analysisAdd BLAST | 38 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 29 ↔ 87 | By similarity | ||
Disulfide bondi | 36 ↔ 42 | By similarity | ||
Disulfide bondi | 52 ↔ 72 | By similarity | ||
Glycosylationi | 59 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 89 ↔ 99 | By similarity | ||
Disulfide bondi | 154 ↔ 214 | By similarity | ||
Disulfide bondi | 161 ↔ 167 | By similarity | ||
Disulfide bondi | 178 ↔ 192 | By similarity | ||
Disulfide bondi | 187 ↔ 233 | By similarity | ||
Disulfide bondi | 216 ↔ 221 | By similarity | ||
Disulfide bondi | 243 ↔ 313 | By similarity | ||
Disulfide bondi | 250 ↔ 256 | By similarity | ||
Disulfide bondi | 267 ↔ 285 | By similarity | ||
Disulfide bondi | 277 ↔ 337 | By similarity | ||
Disulfide bondi | 315 ↔ 325 | By similarity | ||
Glycosylationi | 347 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 406 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Lipidationi | 430 | GPI-anchor amidated serineSequence analysis | 1 |
Keywords - PTMi
Disulfide bond, Glycoprotein, GPI-anchor, LipoproteinProteomic databases
MaxQBi | P97785 |
PaxDbi | P97785 |
PeptideAtlasi | P97785 |
PRIDEi | P97785 |
ProteomicsDBi | 268866 [P97785-1] 268867 [P97785-2] |
PTM databases
GlyGeni | P97785, 3 sites |
iPTMneti | P97785 |
PhosphoSitePlusi | P97785 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSMUSG00000025089, Expressed in vestibular membrane of cochlear duct and 265 other tissues |
ExpressionAtlasi | P97785, baseline and differential |
Genevisiblei | P97785, MM |
Interactioni
Subunit structurei
2 molecules of GDNFR-alpha are thought to form a complex with the disulfide-linked GDNF dimer and with 2 molecules of RET (By similarity).
Interacts with RET (By similarity).
Interacts with SORL1, either alone or in complex with GDNF. Interaction between SORL1 and GFRA1 leads to GFRA1 internalization, but not degradation (By similarity).
By similarityGO - Molecular functioni
- integrin binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 199904, 4 interactors |
CORUMi | P97785 |
IntActi | P97785, 1 interactor |
STRINGi | 10090.ENSMUSP00000026076 |
Miscellaneous databases
RNActi | P97785, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 25 – 113 | 1Add BLAST | 89 | |
Repeati | 150 – 238 | 2Add BLAST | 89 | |
Repeati | 239 – 342 | 3Add BLAST | 104 |
Sequence similaritiesi
Keywords - Domaini
Repeat, SignalPhylogenomic databases
eggNOGi | ENOG502QQA2, Eukaryota |
GeneTreei | ENSGT00940000155560 |
InParanoidi | P97785 |
OMAi | FIPVEHI |
OrthoDBi | 482696at2759 |
PhylomeDBi | P97785 |
TreeFami | TF331647 |
Family and domain databases
InterProi | View protein in InterPro IPR016017, GDNF/GAS1 IPR037193, GDNF_alpha IPR003438, GDNF_rcpt IPR003503, GDNF_rcpt_A1 IPR017372, Glial_neurotroph_fac_rcpt_a1/2 |
PANTHERi | PTHR10269, PTHR10269, 1 hit |
Pfami | View protein in Pfam PF02351, GDNF, 3 hits |
PIRSFi | PIRSF038071, GDNF_family_receptor_alpha, 1 hit |
PRINTSi | PR01317, GDNFRALPHA1 PR01316, GDNFRECEPTOR |
SMARTi | View protein in SMART SM00907, GDNF, 3 hits |
SUPFAMi | SSF110035, SSF110035, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 5 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MFLATLYFVL PLLDLLMSAE VSGGDRLDCV KASDQCLKEQ SCSTKYRTLR
60 70 80 90 100
QCVAGKETNF SLTSGLEAKD ECRSAMEALK QKSLYNCRCK RGMKKEKNCL
110 120 130 140 150
RIYWSMYQSL QGNDLLEDSP YEPVNSRLSD IFRAVPFISD VFQQVEHISK
160 170 180 190 200
GNNCLDAAKA CNLDDTCKKY RSAYITPCTT SMSNEVCNRR KCHKALRQFF
210 220 230 240 250
DKVPAKHSYG MLFCSCRDVA CTERRRQTIV PVCSYEERER PNCLNLQDSC
260 270 280 290 300
KTNYICRSRL ADFFTNCQPE SRSVSNCLKE NYADCLLAYS GLIGTVMTPN
310 320 330 340 350
YIDSSSLSVA PWCDCSNSGN DLEDCLKFLN FFKDNTCLKN AIQAFGNGSD
360 370 380 390 400
VTMWQPAPPV QTTTATTTTA FRIKNKPLGP AGSENEIPTH VLPPCANLQA
410 420 430 440 450
QKLKSNVSGS THLCLSDNDY GKDGLAGASS HITTKSMAAP PSCGLSSLPV
460
MVFTALAALL SVSLAETS
Computationally mapped potential isoform sequencesi
There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketD6RDD4 | D6RDD4_MOUSE | GDNF family receptor alpha-1 | Gfra1 | 419 | Annotation score: | ||
D3YUD5 | D3YUD5_MOUSE | GDNF family receptor alpha-1 | Gfra1 | 54 | Annotation score: | ||
D3Z3C9 | D3Z3C9_MOUSE | GDNF family receptor alpha-1 | Gfra1 | 48 | Annotation score: | ||
D3YWL3 | D3YWL3_MOUSE | GDNF family receptor alpha-1 | Gfra1 | 25 | Annotation score: | ||
A0A1C7ZN00 | A0A1C7ZN00_MOUSE | GDNF family receptor alpha-1 | Gfra1 | 8 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 366 | T → M in BAA19185 (Ref. 2) Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_041630 | 140 – 144 | Missing in isoform 2. 1 Publication | 5 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF014117 mRNA Translation: AAB86599.1 AF015172 mRNA Translation: AAB86600.1 AB000800 mRNA Translation: BAA19185.1 CH466585 Genomic DNA Translation: EDL01795.1 CH466585 Genomic DNA Translation: EDL01796.1 BC054378 mRNA Translation: AAH54378.1 |
CCDSi | CCDS38028.1 [P97785-1] CCDS70967.1 [P97785-2] |
RefSeqi | NP_001272386.1, NM_001285457.2 [P97785-2] NP_034409.1, NM_010279.3 [P97785-1] XP_006526744.1, XM_006526681.1 [P97785-1] XP_006526745.1, XM_006526682.1 [P97785-1] XP_006526746.1, XM_006526683.3 [P97785-1] XP_006526747.1, XM_006526684.1 [P97785-1] |
Genome annotation databases
Ensembli | ENSMUST00000026076; ENSMUSP00000026076; ENSMUSG00000025089 [P97785-1] ENSMUST00000129100; ENSMUSP00000117196; ENSMUSG00000025089 [P97785-2] ENSMUST00000152507; ENSMUSP00000120333; ENSMUSG00000025089 [P97785-1] ENSMUST00000169850; ENSMUSP00000130128; ENSMUSG00000025089 [P97785-1] |
GeneIDi | 14585 |
KEGGi | mmu:14585 |
UCSCi | uc008ial.2, mouse [P97785-2] uc008ian.2, mouse [P97785-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF014117 mRNA Translation: AAB86599.1 AF015172 mRNA Translation: AAB86600.1 AB000800 mRNA Translation: BAA19185.1 CH466585 Genomic DNA Translation: EDL01795.1 CH466585 Genomic DNA Translation: EDL01796.1 BC054378 mRNA Translation: AAH54378.1 |
CCDSi | CCDS38028.1 [P97785-1] CCDS70967.1 [P97785-2] |
RefSeqi | NP_001272386.1, NM_001285457.2 [P97785-2] NP_034409.1, NM_010279.3 [P97785-1] XP_006526744.1, XM_006526681.1 [P97785-1] XP_006526745.1, XM_006526682.1 [P97785-1] XP_006526746.1, XM_006526683.3 [P97785-1] XP_006526747.1, XM_006526684.1 [P97785-1] |
3D structure databases
SMRi | P97785 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 199904, 4 interactors |
CORUMi | P97785 |
IntActi | P97785, 1 interactor |
STRINGi | 10090.ENSMUSP00000026076 |
PTM databases
GlyGeni | P97785, 3 sites |
iPTMneti | P97785 |
PhosphoSitePlusi | P97785 |
Proteomic databases
MaxQBi | P97785 |
PaxDbi | P97785 |
PeptideAtlasi | P97785 |
PRIDEi | P97785 |
ProteomicsDBi | 268866 [P97785-1] 268867 [P97785-2] |
Protocols and materials databases
Antibodypediai | 18697, 460 antibodies from 38 providers |
DNASUi | 14585 |
Genome annotation databases
Ensembli | ENSMUST00000026076; ENSMUSP00000026076; ENSMUSG00000025089 [P97785-1] ENSMUST00000129100; ENSMUSP00000117196; ENSMUSG00000025089 [P97785-2] ENSMUST00000152507; ENSMUSP00000120333; ENSMUSG00000025089 [P97785-1] ENSMUST00000169850; ENSMUSP00000130128; ENSMUSG00000025089 [P97785-1] |
GeneIDi | 14585 |
KEGGi | mmu:14585 |
UCSCi | uc008ial.2, mouse [P97785-2] uc008ian.2, mouse [P97785-1] |
Organism-specific databases
CTDi | 2674 |
MGIi | MGI:1100842, Gfra1 |
VEuPathDBi | HostDB:ENSMUSG00000025089 |
Phylogenomic databases
eggNOGi | ENOG502QQA2, Eukaryota |
GeneTreei | ENSGT00940000155560 |
InParanoidi | P97785 |
OMAi | FIPVEHI |
OrthoDBi | 482696at2759 |
PhylomeDBi | P97785 |
TreeFami | TF331647 |
Enzyme and pathway databases
Reactomei | R-MMU-5673001, RAF/MAP kinase cascade R-MMU-8853659, RET signaling |
Miscellaneous databases
BioGRID-ORCSi | 14585, 1 hit in 63 CRISPR screens |
ChiTaRSi | Gfra1, mouse |
PROi | PR:P97785 |
RNActi | P97785, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000025089, Expressed in vestibular membrane of cochlear duct and 265 other tissues |
ExpressionAtlasi | P97785, baseline and differential |
Genevisiblei | P97785, MM |
Family and domain databases
InterProi | View protein in InterPro IPR016017, GDNF/GAS1 IPR037193, GDNF_alpha IPR003438, GDNF_rcpt IPR003503, GDNF_rcpt_A1 IPR017372, Glial_neurotroph_fac_rcpt_a1/2 |
PANTHERi | PTHR10269, PTHR10269, 1 hit |
Pfami | View protein in Pfam PF02351, GDNF, 3 hits |
PIRSFi | PIRSF038071, GDNF_family_receptor_alpha, 1 hit |
PRINTSi | PR01317, GDNFRALPHA1 PR01316, GDNFRECEPTOR |
SMARTi | View protein in SMART SM00907, GDNF, 3 hits |
SUPFAMi | SSF110035, SSF110035, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | GFRA1_MOUSE | |
Accessioni | P97785Primary (citable) accession number: P97785 Secondary accession number(s): O35246, O35252 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | July 27, 2011 | |
Last modified: | February 23, 2022 | |
This is version 148 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families