UniProtKB - P97742 (CPT1A_MOUSE)
Protein
Carnitine O-palmitoyltransferase 1, liver isoform
Gene
Cpt1a
Organism
Mus musculus (Mouse)
Status
Functioni
Catalyzes the transfer of the acyl group of long-chain fatty acid-CoA conjugates onto carnitine, an essential step for the mitochondrial uptake of long-chain fatty acids and their subsequent beta-oxidation in the mitochondrion (PubMed:16169268, PubMed:17030509). Plays an important role in hepatic triglyceride metabolism (By similarity).By similarity2 Publications
Catalytic activityi
Activity regulationi
Inhibited by malonyl-CoA.1 Publication
: fatty acid beta-oxidation Pathwayi
This protein is involved in the pathway fatty acid beta-oxidation, which is part of Lipid metabolism.View all proteins of this organism that are known to be involved in the pathway fatty acid beta-oxidation and in Lipid metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 473 | Proton acceptorBy similarity | 1 | |
Binding sitei | 589 | CarnitineBy similarity | 1 | |
Binding sitei | 602 | CarnitineBy similarity | 1 |
GO - Molecular functioni
- carnitine O-palmitoyltransferase activity Source: UniProtKB
- identical protein binding Source: MGI
- palmitoleoyltransferase activity Source: UniProtKB
GO - Biological processi
- carnitine metabolic process Source: UniProtKB
- cellular response to fatty acid Source: Ensembl
- eating behavior Source: MGI
- epithelial cell differentiation Source: Ensembl
- fatty acid beta-oxidation Source: UniProtKB-UniPathway
- fatty acid metabolic process Source: GO_Central
- glucose metabolic process Source: MGI
- lipid localization Source: Ensembl
- long-chain fatty acid metabolic process Source: UniProtKB
- positive regulation of fatty acid beta-oxidation Source: MGI
- regulation of fatty acid oxidation Source: MGI
- regulation of insulin secretion Source: MGI
- regulation of lipid storage Source: Ensembl
- response to drug Source: Ensembl
- response to ethanol Source: Ensembl
- response to organic cyclic compound Source: Ensembl
- triglyceride metabolic process Source: MGI
Keywordsi
Molecular function | Acyltransferase, Transferase |
Biological process | Fatty acid metabolism, Lipid metabolism, Transport |
Enzyme and pathway databases
Reactomei | R-MMU-200425, Carnitine metabolism R-MMU-5362517, Signaling by Retinoic Acid |
UniPathwayi | UPA00659 |
Names & Taxonomyi
Protein namesi | Recommended name: Carnitine O-palmitoyltransferase 1, liver isoform (EC:2.3.1.212 Publications)Short name: CPT1-L Alternative name(s): Carnitine O-palmitoyltransferase I, liver isoform Short name: CPT I Short name: CPTI-L Carnitine palmitoyltransferase 1A |
Gene namesi | Name:Cpt1a Synonyms:Cpt-1, Cpt1 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1098296, Cpt1a |
Subcellular locationi
Mitochondrion
- Mitochondrion outer membrane By similarity; Multi-pass membrane protein Sequence analysis
Mitochondrion
- integral component of mitochondrial outer membrane Source: UniProtKB
- mitochondrial inner membrane Source: MGI
- mitochondrial outer membrane Source: UniProtKB
- mitochondrion Source: MGI
Other locations
- integral component of membrane Source: MGI
- intracellular membrane-bounded organelle Source: MGI
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 2 – 47 | CytoplasmicSequence analysisAdd BLAST | 46 | |
Transmembranei | 48 – 73 | HelicalSequence analysisAdd BLAST | 26 | |
Topological domaini | 74 – 102 | Mitochondrial intermembraneSequence analysisAdd BLAST | 29 | |
Transmembranei | 103 – 122 | HelicalSequence analysisAdd BLAST | 20 | |
Topological domaini | 123 – 773 | CytoplasmicSequence analysisAdd BLAST | 651 |
Keywords - Cellular componenti
Membrane, Mitochondrion, Mitochondrion outer membranePathology & Biotechi
Disruption phenotypei
Complete lethality during early embryogenesis. Heterozygous mice are prone to liver steatosis. Compared to wild-type, heterozygotes show minor differences in blood glucose levels and in serum free fatty acid levels after fasting.2 Publications
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedBy similarity | |||
ChainiPRO_0000210160 | 2 – 773 | Carnitine O-palmitoyltransferase 1, liver isoformAdd BLAST | 772 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylalanineBy similarity | 1 | |
Modified residuei | 282 | 3'-nitrotyrosineBy similarity | 1 | |
Modified residuei | 588 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 589 | 3'-nitrotyrosineBy similarity | 1 | |
Modified residuei | 604 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 741 | PhosphoserineBy similarity | 1 | |
Modified residuei | 747 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
Acetylation, Nitration, PhosphoproteinProteomic databases
EPDi | P97742 |
jPOSTi | P97742 |
PaxDbi | P97742 |
PeptideAtlasi | P97742 |
PRIDEi | P97742 |
PTM databases
iPTMneti | P97742 |
PhosphoSitePlusi | P97742 |
SwissPalmi | P97742 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000024900, Expressed in adrenal gland and 347 other tissues |
ExpressionAtlasi | P97742, baseline and differential |
Genevisiblei | P97742, MM |
Interactioni
Subunit structurei
Homohexamer and homotrimer (By similarity).
Identified in a complex that contains at least CPT1A, ACSL1 and VDAC1 (By similarity). Also identified in complexes with ACSL1 and VDAC2 and VDAC3 (By similarity).
By similarityGO - Molecular functioni
- identical protein binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 198863, 4 interactors |
IntActi | P97742, 1 interactor |
STRINGi | 10090.ENSMUSP00000025835 |
Miscellaneous databases
RNActi | P97742, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 555 – 567 | Coenzyme A bindingBy similarityAdd BLAST | 13 |
Domaini
A conformation change in the N-terminal region spanning the first 42 residues plays an important role in the regulation of enzyme activity by malonyl-CoA.By similarity
Sequence similaritiesi
Belongs to the carnitine/choline acetyltransferase family.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG3716, Eukaryota |
GeneTreei | ENSGT00960000186621 |
HOGENOMi | CLU_013513_2_1_1 |
InParanoidi | P97742 |
OMAi | ASHMWEN |
OrthoDBi | 559299at2759 |
PhylomeDBi | P97742 |
TreeFami | TF313836 |
Family and domain databases
Gene3Di | 3.30.559.40, 1 hit 3.30.559.70, 1 hit |
InterProi | View protein in InterPro IPR000542, Carn_acyl_trans IPR039551, Cho/carn_acyl_trans IPR042232, Cho/carn_acyl_trans_1 IPR042231, Cho/carn_acyl_trans_2 IPR032476, CPT_N |
PANTHERi | PTHR22589, PTHR22589, 1 hit |
Pfami | View protein in Pfam PF00755, Carn_acyltransf, 1 hit PF16484, CPT_N, 1 hit |
PROSITEi | View protein in PROSITE PS00439, ACYLTRANSF_C_1, 1 hit PS00440, ACYLTRANSF_C_2, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All
P97742-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAEAHQAVAF QFTVTPDGID LRLSHEALKQ ICLSGLHSWK KKFIRFKNGI
60 70 80 90 100
ITGVFPASPS SWLIVVVGVI SSMHTKVDPS LGMIAKINRT LDTTGRMSSQ
110 120 130 140 150
TKNIVSGVLF GTGLWVAIIM TMRYSLKVLL SYHGWMFAEH GKMSRSTRIW
160 170 180 190 200
MAMVKVFSGR KPMLYSFQTS LPRLPVPAVK DTVSRYLESV RPLMKEGDFQ
210 220 230 240 250
RMTALAQDFA VNLGPKLQWY LKLKSWWATN YVSDWWEEYI YLRGRGPIMV
260 270 280 290 300
NSNYYAMEML YITPTHIQAA RAGNTIHAIL LYRRTVDREE LKPIRLLGST
310 320 330 340 350
IPLCSAQWER LFNTSRIPGE ETDTIQHVKD SRHIVVYHRG RYFKVWLYHD
360 370 380 390 400
GRLLRPRELE QQMQQILDDT SEPQPGEAKL AALTAADRVP WAKCRQTYFA
410 420 430 440 450
RGKNKQSLDA VEKAAFFVTL DESEQGYREE DPEASIDSYA KSLLHGRCFD
460 470 480 490 500
RWFDKSITFV VFKNSKIGIN AEHSWADAPI VGHLWEYVMA TDVFQLGYSE
510 520 530 540 550
DGHCKGDKNP NIPKPTRLQW DIPGECQEVI ETSLSSASFL ANDVDLHSFP
560 570 580 590 600
FDTFGKGLIK KCRTSPDAFI QLALQLAHYK DMGKFCLTYE ASMTRLFREG
610 620 630 640 650
RTETVRSCTT ESCNFVLAMM DPTTTAEQRF KLFKIACEKH QHLYRLAMTG
660 670 680 690 700
AGIDRHLFCL YVVSKYLAVD SPFLKEVLSE PWRLSTSQTP QQQVELFDFE
710 720 730 740 750
KYPDYVSCGG GFGPVADDGY GVSYIIVGEN FIHFHISSKF SSPETDSHRF
760 770
GKHLRQAMMD IITLFGLTAN SKK
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A494BAD3 | A0A494BAD3_MOUSE | Carnitine O-palmitoyltransferase | Cpt1a | 401 | Annotation score: | ||
A0A494BAC7 | A0A494BAC7_MOUSE | Carnitine O-palmitoyltransferase | Cpt1a | 251 | Annotation score: | ||
A0A494BBF2 | A0A494BBF2_MOUSE | Carnitine O-palmitoyltransferase 1,... | Cpt1a | 185 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 145 | R → C in AAH38395 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 145 | R → C in AAH46383 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 398 | Y → C in AAC31641 (PubMed:9680378).Curated | 1 | |
Sequence conflicti | 531 | E → D in BAC36808 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 630 | F → L in AAC31641 (PubMed:9680378).Curated | 1 | |
Sequence conflicti | 732 | I → T in AAC31641 (PubMed:9680378).Curated | 1 | |
Sequence conflicti | 760 | D → A in AAC31641 (PubMed:9680378).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK050213 mRNA Translation: BAC34126.2 AK077454 mRNA Translation: BAC36808.1 BC038395 mRNA Translation: AAH38395.1 BC046383 mRNA Translation: AAH46383.1 AF017175 mRNA Translation: AAC31641.1 S82796 mRNA Translation: AAB39370.1 |
CCDSi | CCDS29395.1 |
RefSeqi | NP_038523.2, NM_013495.2 XP_006531717.1, XM_006531654.2 XP_006531718.1, XM_006531655.2 XP_006531721.1, XM_006531658.3 XP_006531722.1, XM_006531659.2 |
Genome annotation databases
Ensembli | ENSMUST00000025835; ENSMUSP00000025835; ENSMUSG00000024900 |
GeneIDi | 12894 |
KEGGi | mmu:12894 |
UCSCi | uc008fwf.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK050213 mRNA Translation: BAC34126.2 AK077454 mRNA Translation: BAC36808.1 BC038395 mRNA Translation: AAH38395.1 BC046383 mRNA Translation: AAH46383.1 AF017175 mRNA Translation: AAC31641.1 S82796 mRNA Translation: AAB39370.1 |
CCDSi | CCDS29395.1 |
RefSeqi | NP_038523.2, NM_013495.2 XP_006531717.1, XM_006531654.2 XP_006531718.1, XM_006531655.2 XP_006531721.1, XM_006531658.3 XP_006531722.1, XM_006531659.2 |
3D structure databases
SMRi | P97742 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 198863, 4 interactors |
IntActi | P97742, 1 interactor |
STRINGi | 10090.ENSMUSP00000025835 |
PTM databases
iPTMneti | P97742 |
PhosphoSitePlusi | P97742 |
SwissPalmi | P97742 |
Proteomic databases
EPDi | P97742 |
jPOSTi | P97742 |
PaxDbi | P97742 |
PeptideAtlasi | P97742 |
PRIDEi | P97742 |
Protocols and materials databases
Antibodypediai | 1640, 410 antibodies |
DNASUi | 12894 |
Genome annotation databases
Ensembli | ENSMUST00000025835; ENSMUSP00000025835; ENSMUSG00000024900 |
GeneIDi | 12894 |
KEGGi | mmu:12894 |
UCSCi | uc008fwf.2, mouse |
Organism-specific databases
CTDi | 1374 |
MGIi | MGI:1098296, Cpt1a |
Phylogenomic databases
eggNOGi | KOG3716, Eukaryota |
GeneTreei | ENSGT00960000186621 |
HOGENOMi | CLU_013513_2_1_1 |
InParanoidi | P97742 |
OMAi | ASHMWEN |
OrthoDBi | 559299at2759 |
PhylomeDBi | P97742 |
TreeFami | TF313836 |
Enzyme and pathway databases
UniPathwayi | UPA00659 |
Reactomei | R-MMU-200425, Carnitine metabolism R-MMU-5362517, Signaling by Retinoic Acid |
Miscellaneous databases
BioGRID-ORCSi | 12894, 1 hit in 17 CRISPR screens |
ChiTaRSi | Cpt1a, mouse |
PROi | PR:P97742 |
RNActi | P97742, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000024900, Expressed in adrenal gland and 347 other tissues |
ExpressionAtlasi | P97742, baseline and differential |
Genevisiblei | P97742, MM |
Family and domain databases
Gene3Di | 3.30.559.40, 1 hit 3.30.559.70, 1 hit |
InterProi | View protein in InterPro IPR000542, Carn_acyl_trans IPR039551, Cho/carn_acyl_trans IPR042232, Cho/carn_acyl_trans_1 IPR042231, Cho/carn_acyl_trans_2 IPR032476, CPT_N |
PANTHERi | PTHR22589, PTHR22589, 1 hit |
Pfami | View protein in Pfam PF00755, Carn_acyltransf, 1 hit PF16484, CPT_N, 1 hit |
PROSITEi | View protein in PROSITE PS00439, ACYLTRANSF_C_1, 1 hit PS00440, ACYLTRANSF_C_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CPT1A_MOUSE | |
Accessioni | P97742Primary (citable) accession number: P97742 Secondary accession number(s): O35288 Q8C7H8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | July 5, 2004 | |
Last modified: | December 2, 2020 | |
This is version 174 of the entry and version 4 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families