Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Smoothened homolog

Gene

Smo

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

G protein-coupled receptor that probably associates with the patched protein (PTCH) to transduce the hedgehog's proteins signal. Binding of sonic hedgehog (SHH) to its receptor patched is thought to prevent normal inhibition by patched of smoothened (SMO). Required for the accumulation of KIF7, GLI2 and GLI3 in the cilia. Interacts with DLG5 at the ciliary base to induce the accumulation of KIF7 and GLI2 at the ciliary tip for GLI2 activation.By similarity

GO - Molecular functioni

GO - Biological processi

  • axon extension involved in axon guidance Source: RGD
  • commissural neuron axon guidance Source: RGD
  • determination of left/right asymmetry in lateral mesoderm Source: UniProtKB
  • facial nerve development Source: RGD
  • forebrain morphogenesis Source: UniProtKB
  • heart looping Source: UniProtKB
  • mesenchymal to epithelial transition involved in metanephric renal vesicle formation Source: UniProtKB
  • midgut development Source: UniProtKB
  • negative regulation of DNA binding Source: UniProtKB
  • negative regulation of gene expression Source: UniProtKB
  • negative regulation of neuron apoptotic process Source: RGD
  • negative regulation of neuron death Source: RGD
  • neuron projection regeneration Source: RGD
  • osteoblast differentiation Source: RGD
  • osteoblast proliferation Source: RGD
  • positive regulation of branching involved in ureteric bud morphogenesis Source: UniProtKB
  • positive regulation of gene expression Source: UniProtKB
  • positive regulation of hh target transcription factor activity Source: UniProtKB
  • positive regulation of neural precursor cell proliferation Source: RGD
  • positive regulation of vascular associated smooth muscle cell migration Source: RGD
  • response to organic substance Source: RGD
  • response to wounding Source: RGD
  • smoothened signaling pathway Source: UniProtKB
  • somite development Source: UniProtKB
  • spermatogenesis Source: RGD

Keywordsi

Molecular functionDevelopmental protein, G-protein coupled receptor, Receptor, Transducer

Names & Taxonomyi

Protein namesi
Recommended name:
Smoothened homolog
Short name:
SMO
Gene namesi
Name:Smo
Synonyms:Smoh
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi3726 Smo

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini31 – 237ExtracellularSequence analysisAdd BLAST207
Transmembranei238 – 258Helical; Name=1Sequence analysisAdd BLAST21
Topological domaini259 – 266CytoplasmicSequence analysis8
Transmembranei267 – 287Helical; Name=2Sequence analysisAdd BLAST21
Topological domaini288 – 318ExtracellularSequence analysisAdd BLAST31
Transmembranei319 – 339Helical; Name=3Sequence analysisAdd BLAST21
Topological domaini340 – 362CytoplasmicSequence analysisAdd BLAST23
Transmembranei363 – 383Helical; Name=4Sequence analysisAdd BLAST21
Topological domaini384 – 406ExtracellularSequence analysisAdd BLAST23
Transmembranei407 – 427Helical; Name=5Sequence analysisAdd BLAST21
Topological domaini428 – 455CytoplasmicSequence analysisAdd BLAST28
Transmembranei456 – 476Helical; Name=6Sequence analysisAdd BLAST21
Topological domaini477 – 528ExtracellularSequence analysisAdd BLAST52
Transmembranei529 – 549Helical; Name=7Sequence analysisAdd BLAST21
Topological domaini550 – 793CytoplasmicSequence analysisAdd BLAST244

Keywords - Cellular componenti

Cell projection, Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL1795175

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 30Sequence analysisAdd BLAST30
ChainiPRO_000001301731 – 793Smoothened homologAdd BLAST763

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi38N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi74 ↔ 138PROSITE-ProRule annotation
Disulfide bondi82 ↔ 131PROSITE-ProRule annotation
Disulfide bondi122 ↔ 158PROSITE-ProRule annotation
Disulfide bondi151 ↔ 173PROSITE-ProRule annotation
Glycosylationi192N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi197 ↔ 217PROSITE-ProRule annotation
Disulfide bondi221 ↔ 299PROSITE-ProRule annotation
Disulfide bondi318 ↔ 394PROSITE-ProRule annotation
Disulfide bondi494 ↔ 511PROSITE-ProRule annotation
Glycosylationi497N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP97698
PRIDEiP97698

PTM databases

iPTMnetiP97698
PhosphoSitePlusiP97698

Expressioni

Tissue specificityi

In embryo, found in the early neural folds and neural tube, pre-somitic mesoderm and somites, developing limb bud, gut, eye, testes, cartilage, muscle, lung, epiglottis, thymus, tongue, jaw, taste buds, teeth, and skin. In adult, found in multiple tissues including heart, brain, liver, lung, skeletal muscle, kidney and testis.

Interactioni

Subunit structurei

Homodimer. Interacts with ARRB2. Interacts with KIF7. Interacts with BBS5 and BBS7; the interactions are indicative for the association of SMO with the BBsome complex to facilitate ciliary localization of SMO. Interacts with DLG5 and SDCBP. Interacts with GAS8/DRC4.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000011356

Structurei

3D structure databases

ProteinModelPortaliP97698
SMRiP97698
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini69 – 185FZPROSITE-ProRule annotationAdd BLAST117

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni542 – 573Interaction with BBS5 and BBS7By similarityAdd BLAST32
Regioni585 – 597Interaction with DLG5By similarityAdd BLAST13

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3577 Eukaryota
ENOG410XRC8 LUCA
HOGENOMiHOG000252964
HOVERGENiHBG000352
InParanoidiP97698
KOiK06226
PhylomeDBiP97698

Family and domain databases

CDDicd15030 7tmF_SMO_homolog, 1 hit
Gene3Di1.10.2000.10, 1 hit
InterProiView protein in InterPro
IPR015526 Frizzled/SFRP
IPR000539 Frizzled/Smoothened_TM
IPR020067 Frizzled_dom
IPR036790 Frizzled_dom_sf
IPR017981 GPCR_2-like
IPR026544 SMO
IPR035683 SMO_7TM
PANTHERiPTHR11309 PTHR11309, 1 hit
PTHR11309:SF35 PTHR11309:SF35, 1 hit
PfamiView protein in Pfam
PF01534 Frizzled, 1 hit
PF01392 Fz, 1 hit
PRINTSiPR00489 FRIZZLED
SMARTiView protein in SMART
SM00063 FRI, 1 hit
SM01330 Frizzled, 1 hit
SUPFAMiSSF63501 SSF63501, 1 hit
PROSITEiView protein in PROSITE
PS50038 FZ, 1 hit
PS50261 G_PROTEIN_RECEP_F2_4, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P97698-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAGRPVRGP ELAPRRLLQL LLLVLLGGRG RGAALSGNVT GPGPRSAGGS
60 70 80 90 100
ARRNAPVTSP PPPLLSHCGR AAHCEPLRYN VCLGSALPYG ATTTLLAGDS
110 120 130 140 150
DSQEEAHSKL VLWSGLRNAP RCWAVIQPLL CAVYMPKCEN DRVELPSRTL
160 170 180 190 200
CQATRGPCAI VERERGWPDF LRCTPDHFPE GCPNEVQNIK FNSSGQCEAP
210 220 230 240 250
LVRTDNPKSW YEDVEGCGIQ CQNPLFTEAE HQDMHSYIAA FGAVTGLCTL
260 270 280 290 300
FTLATFVADW RNSNRYPAVI LFYVNACFFV GSIGWLAQFM DGARREIVCR
310 320 330 340 350
ADGTMRFGEP TSSETLSCVI IFVIVYYALM AGVVWFVVLT YAWHTSFKAL
360 370 380 390 400
GTTYQPLSGK TSYFHLLTWS LPFVLTVAIL AVAQVDGDSV SGICFVGYKN
410 420 430 440 450
YRYRAGFVLA PIGLVLIVGG YFLIRGVMTL FSIKSNHPGL LSEKAASKIN
460 470 480 490 500
ETMLRLGIFG FLAFGFVLIT FSCHFYDFFN QAEWERSFRD YVLCQANVTI
510 520 530 540 550
GLPTKKPIPD CEIKNRPSLL VEKINLFAMF GTGIAMSTWV WTKATLLIWR
560 570 580 590 600
RTWCRLTGHS DDEPKRIKKS KMIAKAFSKR RELLQNPGQE LSFSMHTVSH
610 620 630 640 650
DGPVAGLAFE LNEPSADVSS AWAQHVTKMV ARRGAILPQD VSVTPVATPV
660 670 680 690 700
PPEEQANLWL VEAEISPELE KRLGRKKKRR KRKKEVCPLG PAPELHHSAP
710 720 730 740 750
VPATSAVPRL PQLPRQKCLV AANAWGTGEP CRQGAWTVVS NPFCPEPSPH
760 770 780 790
QDPFLPGASA PRVWAQGRLQ GLGSIHSRTN LMEAELLDAD SDF
Length:793
Mass (Da):87,376
Last modified:May 1, 1997 - v1
Checksum:i27B88178B2565FB9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U84402 mRNA Translation: AAB41789.1
RefSeqiNP_036939.1, NM_012807.1
UniGeneiRn.44429

Genome annotation databases

GeneIDi25273
KEGGirno:25273
UCSCiRGD:3726 rat

Similar proteinsi

Entry informationi

Entry nameiSMO_RAT
AccessioniPrimary (citable) accession number: P97698
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 1, 1997
Last modified: May 23, 2018
This is version 127 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. SIMILARITY comments
    Index of protein domains and families

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health