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Protein

Inositol monophosphatase 1

Gene

Impa1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Responsible for the provision of inositol required for synthesis of phosphatidylinositol and polyphosphoinositides and has been implicated as the pharmacological target for lithium action in brain. Has broad substrate specificity and can use myo-inositol 1,3-diphosphate, myo-inositol 1,4-diphosphate, scyllo-inositol-phosphate, glucose-1-phosphate, glucose-6-phosphate, fructose-1-phosphate, beta-glycerophosphate, and 2'-AMP as substrates (By similarity). Is equally active with myo-inositol monophosphate and D-galactose 1-phosphate.By similarity1 Publication

Catalytic activityi

Myo-inositol phosphate + H2O = myo-inositol + phosphate.1 Publication
Alpha-D-galactose 1-phosphate + H2O = D-galactose + phosphate.1 Publication

Cofactori

Mg2+1 Publication

Enzyme regulationi

Activity with myo-inositol monophosphate and D-galactose 1-phosphate is inhibited by Li+, Ca2+ and Mn2+, but also by Mg2+ at concentrations above 3 mM.1 Publication

Pathwayi: myo-inositol biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes myo-inositol from D-glucose 6-phosphate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Inositol-3-phosphate synthase 1 (Isyna1)
  2. Inositol-1-monophosphatase (Impa1), Inositol monophosphatase 3 (Impad1), Inositol monophosphatase 1 (Impa1), Inositol-1-monophosphatase (Impa2), Inositol monophosphatase 2 (Impa2)
This subpathway is part of the pathway myo-inositol biosynthesis, which is itself part of Polyol metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes myo-inositol from D-glucose 6-phosphate, the pathway myo-inositol biosynthesis and in Polyol metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi70Magnesium 1; catalyticBy similarity1
Binding sitei70SubstrateBy similarity1
Metal bindingi90Magnesium 1; catalyticBy similarity1
Metal bindingi90Magnesium 2By similarity1
Metal bindingi92Magnesium 1; via carbonyl oxygen; catalyticBy similarity1
Metal bindingi93Magnesium 2By similarity1
Binding sitei213SubstrateBy similarity1
Metal bindingi220Magnesium 2By similarity1
Binding sitei220SubstrateBy similarity1

GO - Molecular functioni

  • inositol monophosphate 1-phosphatase activity Source: RGD
  • inositol monophosphate 3-phosphatase activity Source: UniProtKB-EC
  • inositol monophosphate 4-phosphatase activity Source: UniProtKB-EC
  • inositol monophosphate phosphatase activity Source: UniProtKB
  • lithium ion binding Source: UniProtKB
  • magnesium ion binding Source: UniProtKB
  • manganese ion binding Source: UniProtKB

GO - Biological processi

  • inositol biosynthetic process Source: UniProtKB-UniPathway
  • inositol metabolic process Source: GO_Central
  • inositol phosphate dephosphorylation Source: UniProtKB
  • phosphatidylinositol biosynthetic process Source: RGD
  • phosphatidylinositol phosphorylation Source: InterPro
  • response to lithium ion Source: RGD
  • signal transduction Source: GO_Central

Keywordsi

Molecular functionHydrolase
LigandLithium, Magnesium, Metal-binding

Enzyme and pathway databases

BRENDAi3.1.3.25 5301
SABIO-RKiP97697
UniPathwayiUPA00823; UER00788

Names & Taxonomyi

Protein namesi
Recommended name:
Inositol monophosphatase 1 (EC:3.1.3.251 Publication)
Short name:
IMP 1
Short name:
IMPase 1
Alternative name(s):
D-galactose 1-phosphate phosphatase1 Publication (EC:3.1.3.941 Publication)
Inositol-1(or 4)-monophosphatase 1
Lithium-sensitive myo-inositol monophosphatase A1
Gene namesi
Name:Impa1
Synonyms:Imp
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi69254 Impa1

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL1293238

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001425171 – 277Inositol monophosphatase 1Add BLAST277

Proteomic databases

PaxDbiP97697
PRIDEiP97697

PTM databases

iPTMnetiP97697
PhosphoSitePlusiP97697
SwissPalmiP97697

Expressioni

Tissue specificityi

Ubiquitous.1 Publication

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

BioGridi249742, 1 interactor
MINTiP97697
STRINGi10116.ENSRNOP00000014274

Structurei

3D structure databases

ProteinModelPortaliP97697
SMRiP97697
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni92 – 95Substrate bindingBy similarity4
Regioni194 – 196Substrate bindingBy similarity3

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG2951 Eukaryota
COG0483 LUCA
HOGENOMiHOG000282238
HOVERGENiHBG052123
InParanoidiP97697
KOiK01092
PhylomeDBiP97697

Family and domain databases

CDDicd01639 IMPase, 1 hit
InterProiView protein in InterPro
IPR033942 IMPase
IPR020583 Inositol_monoP_metal-BS
IPR020552 Inositol_monoPase_Li-sen
IPR000760 Inositol_monophosphatase-like
IPR020550 Inositol_monophosphatase_CS
PfamiView protein in Pfam
PF00459 Inositol_P, 1 hit
PRINTSiPR00377 IMPHPHTASES
PR00378 LIIMPHPHTASE
PROSITEiView protein in PROSITE
PS00629 IMP_1, 1 hit
PS00630 IMP_2, 1 hit

Sequencei

Sequence statusi: Complete.

P97697-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADPWQECMD YAVILARQAG EMIREALKNK MDVMIKSSPA DLVTVTDQKV
60 70 80 90 100
EKMLMSSIKE KYPYHSFIGE ESVASGEKTV FTEQPTWIID PIDGTTNFVH
110 120 130 140 150
RFPFVAVSIG FVVNKEMEFG VVYSCVEDKM YTGRKGKGAF CNGQKLRVSQ
160 170 180 190 200
QEDITKSLLV TELGSSRKPE TLRIVLSNME RLCSIPIHGI RSVGTAAVNM
210 220 230 240 250
CLVATGGADA YYEMGIHCWD MAGAGIIVIE AGGVLLDVTG GPFDLMSRRI
260 270
IAASNIALAE RIAKELEIIP LQRDDES
Length:277
Mass (Da):30,511
Last modified:March 1, 2004 - v2
Checksum:i01A7FFB795D2AAED
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U84038 mRNA Translation: AAB63338.2
RefSeqiNP_114446.1, NM_032057.1
UniGeneiRn.3975

Genome annotation databases

GeneIDi83523
KEGGirno:83523
UCSCiRGD:69254 rat

Similar proteinsi

Entry informationi

Entry nameiIMPA1_RAT
AccessioniPrimary (citable) accession number: P97697
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: March 1, 2004
Last modified: May 23, 2018
This is version 143 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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