UniProtKB - P97697 (IMPA1_RAT)
Protein
Inositol monophosphatase 1
Gene
Impa1
Organism
Rattus norvegicus (Rat)
Status
Functioni
Responsible for the provision of inositol required for synthesis of phosphatidylinositol and polyphosphoinositides and has been implicated as the pharmacological target for lithium action in brain. Has broad substrate specificity and can use myo-inositol 1,3-diphosphate, myo-inositol 1,4-diphosphate, scyllo-inositol-phosphate, glucose-1-phosphate, glucose-6-phosphate, fructose-1-phosphate, beta-glycerophosphate, and 2'-AMP as substrates (By similarity). Is equally active with myo-inositol monophosphate and D-galactose 1-phosphate.By similarity1 Publication
Catalytic activityi
Cofactori
Mg2+1 Publication
Activity regulationi
Activity with myo-inositol monophosphate and D-galactose 1-phosphate is inhibited by Li+, Ca2+ and Mn2+, but also by Mg2+ at concentrations above 3 mM.1 Publication
: myo-inositol biosynthesis Pathwayi
This protein is involved in step 2 of the subpathway that synthesizes myo-inositol from D-glucose 6-phosphate.Proteins known to be involved in the 2 steps of the subpathway in this organism are:
- Inositol-3-phosphate synthase 1 (Isyna1)
- Inositol-1-monophosphatase (Impa1), Inositol monophosphatase 2 (Impa2), Inositol monophosphatase 1 (Impa1), Inositol-1-monophosphatase (Impa2)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes myo-inositol from D-glucose 6-phosphate, the pathway myo-inositol biosynthesis and in Polyol metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 70 | Magnesium 1; catalyticBy similarity | 1 | |
Binding sitei | 70 | SubstrateBy similarity | 1 | |
Metal bindingi | 90 | Magnesium 1; catalyticBy similarity | 1 | |
Metal bindingi | 90 | Magnesium 2By similarity | 1 | |
Metal bindingi | 92 | Magnesium 1; via carbonyl oxygen; catalyticBy similarity | 1 | |
Metal bindingi | 93 | Magnesium 2By similarity | 1 | |
Binding sitei | 213 | SubstrateBy similarity | 1 | |
Metal bindingi | 220 | Magnesium 2By similarity | 1 | |
Binding sitei | 220 | SubstrateBy similarity | 1 |
GO - Molecular functioni
- identical protein binding Source: RGD
- inositol monophosphate 1-phosphatase activity Source: RGD
- inositol monophosphate 3-phosphatase activity Source: UniProtKB-EC
- inositol monophosphate 4-phosphatase activity Source: UniProtKB-EC
- inositol monophosphate phosphatase activity Source: UniProtKB
- lithium ion binding Source: UniProtKB
- magnesium ion binding Source: UniProtKB
- manganese ion binding Source: UniProtKB
- protein homodimerization activity Source: RGD
GO - Biological processi
- inositol biosynthetic process Source: UniProtKB-UniPathway
- inositol metabolic process Source: GO_Central
- inositol phosphate dephosphorylation Source: UniProtKB
- phosphate-containing compound metabolic process Source: RGD
- phosphatidylinositol biosynthetic process Source: RGD
- phosphatidylinositol phosphorylation Source: InterPro
- response to lithium ion Source: RGD
- signal transduction Source: RGD
Keywordsi
Molecular function | Hydrolase |
Ligand | Lithium, Magnesium, Metal-binding |
Enzyme and pathway databases
BRENDAi | 3.1.3.25, 5301 |
Reactomei | R-RNO-1855183, Synthesis of IP2, IP, and Ins in the cytosol |
SABIO-RKi | P97697 |
UniPathwayi | UPA00823;UER00788 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:Impa1 Synonyms:Imp |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 69254, Impa1 |
Subcellular locationi
Other locations
- Cytoplasm By similarity
Other locations
- axon Source: BHF-UCL
- cytoplasm Source: RGD
- neuronal cell body Source: BHF-UCL
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000142517 | 1 – 277 | Inositol monophosphatase 1Add BLAST | 277 |
Proteomic databases
jPOSTi | P97697 |
PaxDbi | P97697 |
PRIDEi | P97697 |
PTM databases
iPTMneti | P97697 |
PhosphoSitePlusi | P97697 |
SwissPalmi | P97697 |
Expressioni
Tissue specificityi
Ubiquitous.1 Publication
Interactioni
Subunit structurei
Homodimer.
1 PublicationGO - Molecular functioni
- identical protein binding Source: RGD
- protein homodimerization activity Source: RGD
Protein-protein interaction databases
BioGRIDi | 249742, 1 interactor |
MINTi | P97697 |
STRINGi | 10116.ENSRNOP00000014274 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 92 – 95 | Substrate bindingBy similarity | 4 | |
Regioni | 194 – 196 | Substrate bindingBy similarity | 3 |
Sequence similaritiesi
Belongs to the inositol monophosphatase superfamily.Curated
Phylogenomic databases
eggNOGi | KOG2951, Eukaryota |
InParanoidi | P97697 |
OrthoDBi | 915621at2759 |
PhylomeDBi | P97697 |
Family and domain databases
CDDi | cd01639, IMPase, 1 hit |
InterProi | View protein in InterPro IPR033942, IMPase IPR020583, Inositol_monoP_metal-BS IPR020552, Inositol_monoPase_Li-sen IPR000760, Inositol_monophosphatase-like IPR020550, Inositol_monophosphatase_CS |
Pfami | View protein in Pfam PF00459, Inositol_P, 1 hit |
PRINTSi | PR00377, IMPHPHTASES PR00378, LIIMPHPHTASE |
PROSITEi | View protein in PROSITE PS00629, IMP_1, 1 hit PS00630, IMP_2, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
P97697-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MADPWQECMD YAVILARQAG EMIREALKNK MDVMIKSSPA DLVTVTDQKV
60 70 80 90 100
EKMLMSSIKE KYPYHSFIGE ESVASGEKTV FTEQPTWIID PIDGTTNFVH
110 120 130 140 150
RFPFVAVSIG FVVNKEMEFG VVYSCVEDKM YTGRKGKGAF CNGQKLRVSQ
160 170 180 190 200
QEDITKSLLV TELGSSRKPE TLRIVLSNME RLCSIPIHGI RSVGTAAVNM
210 220 230 240 250
CLVATGGADA YYEMGIHCWD MAGAGIIVIE AGGVLLDVTG GPFDLMSRRI
260 270
IAASNIALAE RIAKELEIIP LQRDDES
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketF1M978 | F1M978_RAT | Inositol-1-monophosphatase | Impa1 | 277 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U84038 mRNA Translation: AAB63338.2 |
RefSeqi | NP_114446.1, NM_032057.1 |
Genome annotation databases
GeneIDi | 83523 |
KEGGi | rno:83523 |
UCSCi | RGD:69254, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U84038 mRNA Translation: AAB63338.2 |
RefSeqi | NP_114446.1, NM_032057.1 |
3D structure databases
SMRi | P97697 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 249742, 1 interactor |
MINTi | P97697 |
STRINGi | 10116.ENSRNOP00000014274 |
Chemistry databases
ChEMBLi | CHEMBL1293238 |
PTM databases
iPTMneti | P97697 |
PhosphoSitePlusi | P97697 |
SwissPalmi | P97697 |
Proteomic databases
jPOSTi | P97697 |
PaxDbi | P97697 |
PRIDEi | P97697 |
Genome annotation databases
GeneIDi | 83523 |
KEGGi | rno:83523 |
UCSCi | RGD:69254, rat |
Organism-specific databases
CTDi | 3612 |
RGDi | 69254, Impa1 |
Phylogenomic databases
eggNOGi | KOG2951, Eukaryota |
InParanoidi | P97697 |
OrthoDBi | 915621at2759 |
PhylomeDBi | P97697 |
Enzyme and pathway databases
UniPathwayi | UPA00823;UER00788 |
BRENDAi | 3.1.3.25, 5301 |
Reactomei | R-RNO-1855183, Synthesis of IP2, IP, and Ins in the cytosol |
SABIO-RKi | P97697 |
Miscellaneous databases
PROi | PR:P97697 |
Family and domain databases
CDDi | cd01639, IMPase, 1 hit |
InterProi | View protein in InterPro IPR033942, IMPase IPR020583, Inositol_monoP_metal-BS IPR020552, Inositol_monoPase_Li-sen IPR000760, Inositol_monophosphatase-like IPR020550, Inositol_monophosphatase_CS |
Pfami | View protein in Pfam PF00459, Inositol_P, 1 hit |
PRINTSi | PR00377, IMPHPHTASES PR00378, LIIMPHPHTASE |
PROSITEi | View protein in PROSITE PS00629, IMP_1, 1 hit PS00630, IMP_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | IMPA1_RAT | |
Accessioni | P97697Primary (citable) accession number: P97697 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 15, 1998 |
Last sequence update: | March 1, 2004 | |
Last modified: | December 2, 2020 | |
This is version 155 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families