UniProtKB - P97694 (CYH1_RAT)
Protein
Cytohesin-1
Gene
Cyth1
Organism
Rattus norvegicus (Rat)
Status
Functioni
Promotes guanine-nucleotide exchange on ARF1, ARF5 and ARF6. Promotes the activation of ARF factors through replacement of GDP with GTP. Plays an important role in membrane trafficking, during junctional remodeling and epithelial polarization, through regulation of ARF6 activity.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 281 | Phosphatidylinositol 3,4,5-trisphosphateBy similarity | 1 | |
Binding sitei | 292 | Phosphatidylinositol 3,4,5-trisphosphateBy similarity | 1 | |
Binding sitei | 302 | Phosphatidylinositol 3,4,5-trisphosphateBy similarity | 1 | |
Binding sitei | 351 | Phosphatidylinositol 3,4,5-trisphosphateBy similarity | 1 |
GO - Molecular functioni
- ARF guanyl-nucleotide exchange factor activity Source: UniProtKB
- lipid binding Source: UniProtKB-KW
GO - Biological processi
- establishment of epithelial cell polarity Source: UniProtKB
- regulation of ARF protein signal transduction Source: InterPro
- regulation of cell adhesion Source: RGD
- regulation of synaptic vesicle exocytosis Source: SynGO
Keywordsi
Molecular function | Guanine-nucleotide releasing factor |
Ligand | Lipid-binding |
Enzyme and pathway databases
Reactomei | R-RNO-6811438, Intra-Golgi traffic |
Names & Taxonomyi
Protein namesi | Recommended name: Cytohesin-1Alternative name(s): PH, SEC7 and coiled-coil domain-containing protein 1 SEC7 homolog A Short name: rSec7-1 |
Gene namesi | Name:Cyth1 Synonyms:Pscd1, Sec7a |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 620397, Cyth1 |
Subcellular locationi
Plasma membrane
- Cell membrane By similarity; Peripheral membrane protein By similarity
Cytosol
- cytosol By similarity
Other locations
- tight junction By similarity
- adherens junction By similarity
Note: Colocalized with TJP1 during epithelial polarization.By similarity
Cytosol
- cytosol Source: UniProtKB
Plasma Membrane
- extrinsic component of cytoplasmic side of plasma membrane Source: UniProtKB
- extrinsic component of presynaptic membrane Source: SynGO
- plasma membrane Source: UniProtKB
Other locations
- adherens junction Source: UniProtKB-SubCell
- bicellular tight junction Source: UniProtKB
- cytoplasm Source: RGD
- neuromuscular junction Source: SynGO
Keywords - Cellular componenti
Cell junction, Cell membrane, Cytoplasm, Membrane, Tight junctionPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 157 | E → K: Loss of ARF translocation. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000120196 | 1 – 398 | Cytohesin-1Add BLAST | 398 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionineBy similarity | 1 |
Post-translational modificationi
Ubiquitinated by SCF(FBXW11) E3 ubiquitin-protein ligase complex. Ubiquitination induces proteasomal degradation.By similarity
Keywords - PTMi
Acetylation, Ubl conjugationProteomic databases
jPOSTi | P97694 |
PaxDbi | P97694 |
PRIDEi | P97694 |
PTM databases
iPTMneti | P97694 |
PhosphoSitePlusi | P97694 |
Expressioni
Tissue specificityi
Present in all tissues tested, with highest protein levels in brain and adrenal.
Developmental stagei
On embryonic days 15 (E15) and E18, expression is seen in the mantle and ventricular germinal zones throughout the neuraxis. On postnatal days 0 (P0) and P7, expression is seen in the cerebral neocortex, olfactory mitral and granule cell layers, hippocampal pyramidal and dentate granule cells and the striatum. A lower expression is seen in gray matter in di-, mes- and met-encephali. In the cerebellum, the expression is evident in the external and internal granule cell layers and Purkinje cell layer. On P14, a decreased expression is seen in the di-, mes- and met-encephali. On P21 and thereafter, expression is seen in the olfactory mitral and granule cells, dentate granule cells and the cerebellar granule cells and Purkinje cells.1 Publication
Interactioni
Subunit structurei
Interacts with TRIM23 and CYTIP (By similarity).
Interacts (via coiled-coil domain) with FRMD4A (via coiled-coil domain) (By similarity).
Interacts with FRMD4B (By similarity).
Found in a complex with PARD3, CYTH1 and FRMD4A (By similarity).
Interacts (via N-terminal domain) with INAVA (via N-terminal domain) (By similarity).
By similarityGO - Molecular functioni
- ARF guanyl-nucleotide exchange factor activity Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 250573, 1 interactor |
STRINGi | 10116.ENSRNOP00000004196 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 73 – 202 | SEC7PROSITE-ProRule annotationAdd BLAST | 130 | |
Domaini | 260 – 377 | PHPROSITE-ProRule annotationAdd BLAST | 118 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 269 – 277 | Phosphatidylinositol 3,4,5-trisphosphate bindingBy similarity | 9 | |
Regioni | 388 – 396 | C-terminal autoinhibitory regionBy similarity | 9 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 10 – 67 | Sequence analysisAdd BLAST | 58 |
Domaini
Binds via its PH domain to the inositol head group of phosphatidylinositol 3,4,5-trisphosphate.By similarity
Autoinhibited by its C-terminal basic region.By similarity
Keywords - Domaini
Coiled coilPhylogenomic databases
eggNOGi | KOG0930, Eukaryota |
InParanoidi | P97694 |
OrthoDBi | 657055at2759 |
PhylomeDBi | P97694 |
Family and domain databases
CDDi | cd00171, Sec7, 1 hit |
Gene3Di | 1.10.1000.11, 1 hit 2.30.29.30, 1 hit |
InterProi | View protein in InterPro IPR011993, PH-like_dom_sf IPR001849, PH_domain IPR023394, Sec7_C_sf IPR000904, Sec7_dom IPR035999, Sec7_dom_sf |
Pfami | View protein in Pfam PF00169, PH, 1 hit PF01369, Sec7, 1 hit |
SMARTi | View protein in SMART SM00233, PH, 1 hit SM00222, Sec7, 1 hit |
SUPFAMi | SSF48425, SSF48425, 1 hit |
PROSITEi | View protein in PROSITE PS50003, PH_DOMAIN, 1 hit PS50190, SEC7, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
P97694-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MEDDDSYVPS DLTAEERQEL ENIRRRKQEL LADIQRLKEE IAEVANEIES
60 70 80 90 100
LGSTEERKNM QRNKQVAMGR KKFNMDPKKG IQFLIENGLL KNTCEDIAQF
110 120 130 140 150
LYKGEGLNKT AIGDYLGERD EFSIQVLHAF VELHEFTDLN LVQALRQFLW
160 170 180 190 200
SFRLPGEAQK IDRMMEAFAQ RYCQCNTGVF QSTDTCYVLS FAIIMLNTSL
210 220 230 240 250
HNPNVKDKPT VERFIAMNRG INDGGDLPEE LLRNLYESIK NEPFKIPEDD
260 270 280 290 300
GNDLTHTFFN PDREGWLLKL GGGRVKTWKR RWFILTDNCL YYFEYTTDKE
310 320 330 340 350
PRGIIPLENL SIREVEDSKK PNCFELYIPD NKDQVIKACK TEADGRVVEG
360 370 380 390
NHTVYRISAP TPEEKEDWIK CIKAAISRDP FYEMLAARKK KVSSTKRH
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketB2GUV0 | B2GUV0_RAT | Cyth1 protein | Cyth1 Usp36 | 397 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U83895 mRNA Translation: AAB41443.1 |
RefSeqi | NP_446362.1, NM_053910.1 |
Genome annotation databases
GeneIDi | 116691 |
KEGGi | rno:116691 |
UCSCi | RGD:620397, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U83895 mRNA Translation: AAB41443.1 |
RefSeqi | NP_446362.1, NM_053910.1 |
3D structure databases
SMRi | P97694 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 250573, 1 interactor |
STRINGi | 10116.ENSRNOP00000004196 |
PTM databases
iPTMneti | P97694 |
PhosphoSitePlusi | P97694 |
Proteomic databases
jPOSTi | P97694 |
PaxDbi | P97694 |
PRIDEi | P97694 |
Genome annotation databases
GeneIDi | 116691 |
KEGGi | rno:116691 |
UCSCi | RGD:620397, rat |
Organism-specific databases
CTDi | 9267 |
RGDi | 620397, Cyth1 |
Phylogenomic databases
eggNOGi | KOG0930, Eukaryota |
InParanoidi | P97694 |
OrthoDBi | 657055at2759 |
PhylomeDBi | P97694 |
Enzyme and pathway databases
Reactomei | R-RNO-6811438, Intra-Golgi traffic |
Miscellaneous databases
PROi | PR:P97694 |
Family and domain databases
CDDi | cd00171, Sec7, 1 hit |
Gene3Di | 1.10.1000.11, 1 hit 2.30.29.30, 1 hit |
InterProi | View protein in InterPro IPR011993, PH-like_dom_sf IPR001849, PH_domain IPR023394, Sec7_C_sf IPR000904, Sec7_dom IPR035999, Sec7_dom_sf |
Pfami | View protein in Pfam PF00169, PH, 1 hit PF01369, Sec7, 1 hit |
SMARTi | View protein in SMART SM00233, PH, 1 hit SM00222, Sec7, 1 hit |
SUPFAMi | SSF48425, SSF48425, 1 hit |
PROSITEi | View protein in PROSITE PS50003, PH_DOMAIN, 1 hit PS50190, SEC7, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CYH1_RAT | |
Accessioni | P97694Primary (citable) accession number: P97694 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 21, 2001 |
Last sequence update: | May 1, 1997 | |
Last modified: | February 10, 2021 | |
This is version 122 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |