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Protein

Fatty-acid amide hydrolase 1

Gene

Faah

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Degrades bioactive fatty acid amides like oleamide, the endogenous cannabinoid, anandamide and myristic amide to their corresponding acids, thereby serving to terminate the signaling functions of these molecules. Hydrolyzes polyunsaturated substrate anandamide preferentially as compared to monounsaturated substrates (By similarity).By similarity

Catalytic activityi

Anandamide + H2O = arachidonic acid + ethanolamine.
Oleamide + H2O = oleic acid + NH3.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei142Charge relay system1
Binding sitei191Substrate; via carbonyl oxygen1
Active sitei217Charge relay system1
Binding sitei217Substrate1
Active sitei241Acyl-ester intermediate1

GO - Molecular functioni

  • amidase activity Source: RGD
  • anandamide amidohydrolase activity Source: UniProtKB-EC
  • fatty acid amide hydrolase activity Source: UniProtKB
  • hydrolase activity, acting on ester bonds Source: RGD
  • identical protein binding Source: IntAct
  • lipid binding Source: RGD
  • oleamide hydrolase activity Source: UniProtKB-EC
  • phospholipid binding Source: RGD
  • protein homodimerization activity Source: RGD

GO - Biological processi

Keywordsi

Molecular functionHydrolase

Enzyme and pathway databases

BRENDAi3.5.1.4 5301
3.5.1.99 5301
ReactomeiR-RNO-2142753 Arachidonic acid metabolism

Chemistry databases

SwissLipidsiSLP:000001428

Names & Taxonomyi

Protein namesi
Recommended name:
Fatty-acid amide hydrolase 1 (EC:3.5.1.99)
Alternative name(s):
Anandamide amidohydrolase 1
Oleamide hydrolase 1
Gene namesi
Name:Faah
Synonyms:Faah1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 5

Organism-specific databases

RGDi61808 Faah

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei9 – 29HelicalSequence analysisAdd BLAST21
Topological domaini30 – 403CytoplasmicCuratedAdd BLAST374
Intramembranei404 – 433CuratedAdd BLAST30
Topological domaini434 – 579CytoplasmicCuratedAdd BLAST146

Keywords - Cellular componenti

Endoplasmic reticulum, Golgi apparatus, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi142K → A: Lowers activity 40000-fold. Lowers activity 70000-fold; when associated with A-217. 1 Publication1
Mutagenesisi217S → A: Lowers activity 3000-fold. Lowers activity 70000-fold; when associated with A-142. 1 Publication1

Chemistry databases

ChEMBLiCHEMBL3229
GuidetoPHARMACOLOGYi1400

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001052671 – 579Fatty-acid amide hydrolase 1Add BLAST579

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei241PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP97612
PRIDEiP97612

PTM databases

iPTMnetiP97612
PhosphoSitePlusiP97612

Expressioni

Tissue specificityi

Found in neuronal cells throughout the CNS. Expressed in liver and brain, and to a lesser extent in spleen, lung, kidney and testes.1 Publication

Developmental stagei

In the CNS it accumulates progressively between embryonic day 14 and postnatal day 10, remains high until postnatal day 30, then decreases into adulthood.

Gene expression databases

BgeeiENSRNOG00000011019 Expressed in 8 organ(s), highest expression level in liver
GenevisibleiP97612 RN

Interactioni

Subunit structurei

Homodimer.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
itself2EBI-15726093,EBI-15726093

GO - Molecular functioni

Protein-protein interaction databases

DIPiDIP-46284N
STRINGi10116.ENSRNOP00000015961

Chemistry databases

BindingDBiP97612

Structurei

Secondary structure

1579
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP97612
SMRiP97612
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP97612

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni238 – 241Substrate binding4

Sequence similaritiesi

Belongs to the amidase family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1212 Eukaryota
COG0154 LUCA
GeneTreeiENSGT00550000074673
HOGENOMiHOG000016500
HOVERGENiHBG005632
InParanoidiP97612
KOiK15528
OMAiTSMPGQE
OrthoDBiEOG091G05JU

Family and domain databases

Gene3Di3.90.1300.10, 1 hit
InterProiView protein in InterPro
IPR000120 Amidase
IPR020556 Amidase_CS
IPR023631 Amidase_dom
IPR036928 AS_sf
IPR030560 FAAH
PANTHERiPTHR11895 PTHR11895, 1 hit
PTHR11895:SF91 PTHR11895:SF91, 1 hit
PfamiView protein in Pfam
PF01425 Amidase, 1 hit
SUPFAMiSSF75304 SSF75304, 1 hit
PROSITEiView protein in PROSITE
PS00571 AMIDASES, 1 hit

Sequencei

Sequence statusi: Complete.

P97612-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MVLSEVWTTL SGVSGVCLAC SLLSAAVVLR WTGRQKARGA ATRARQKQRA
60 70 80 90 100
SLETMDKAVQ RFRLQNPDLD SEALLTLPLL QLVQKLQSGE LSPEAVFFTY
110 120 130 140 150
LGKAWEVNKG TNCVTSYLTD CETQLSQAPR QGLLYGVPVS LKECFSYKGH
160 170 180 190 200
DSTLGLSLNE GMPSESDCVV VQVLKLQGAV PFVHTNVPQS MLSFDCSNPL
210 220 230 240 250
FGQTMNPWKS SKSPGGSSGG EGALIGSGGS PLGLGTDIGG SIRFPSAFCG
260 270 280 290 300
ICGLKPTGNR LSKSGLKGCV YGQTAVQLSL GPMARDVESL ALCLKALLCE
310 320 330 340 350
HLFTLDPTVP PLPFREEVYR SSRPLRVGYY ETDNYTMPSP AMRRALIETK
360 370 380 390 400
QRLEAAGHTL IPFLPNNIPY ALEVLSAGGL FSDGGRSFLQ NFKGDFVDPC
410 420 430 440 450
LGDLILILRL PSWFKRLLSL LLKPLFPRLA AFLNSMRPRS AEKLWKLQHE
460 470 480 490 500
IEMYRQSVIA QWKAMNLDVL LTPMLGPALD LNTPGRATGA ISYTVLYNCL
510 520 530 540 550
DFPAGVVPVT TVTAEDDAQM ELYKGYFGDI WDIILKKAMK NSVGLPVAVQ
560 570
CVALPWQEEL CLRFMREVEQ LMTPQKQPS
Length:579
Mass (Da):63,357
Last modified:May 1, 1997 - v1
Checksum:i606491C0B033E1AA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U72497 mRNA Translation: AAB26961.1
BC091148 mRNA Translation: AAH91148.1
RefSeqiNP_077046.1, NM_024132.3
XP_003750049.1, XM_003750001.4
XP_008774266.1, XM_008776044.2
UniGeneiRn.89119

Genome annotation databases

EnsembliENSRNOT00000015667; ENSRNOP00000015667; ENSRNOG00000045949
ENSRNOT00000015961; ENSRNOP00000015961; ENSRNOG00000011019
GeneIDi100911581
29347
KEGGirno:100911581
rno:29347
UCSCiRGD:61808 rat

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U72497 mRNA Translation: AAB26961.1
BC091148 mRNA Translation: AAH91148.1
RefSeqiNP_077046.1, NM_024132.3
XP_003750049.1, XM_003750001.4
XP_008774266.1, XM_008776044.2
UniGeneiRn.89119

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1MT5X-ray2.80A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P37-573[»]
2VYAX-ray2.75A/B32-579[»]
2WAPX-ray2.80A/B31-573[»]
2WJ1X-ray1.84A/B30-579[»]
2WJ2X-ray2.55A/B30-579[»]
3K7FX-ray1.95A/B30-579[»]
3K83X-ray2.25A/B30-579[»]
3K84X-ray2.25A/B30-579[»]
3LJ6X-ray2.42A/B30-579[»]
3LJ7X-ray2.30A/B30-579[»]
3OJ8X-ray1.90A/B30-579[»]
3PPMX-ray1.78A/B30-579[»]
3PR0X-ray2.20A/B30-579[»]
3QJ8X-ray2.90A/B32-579[»]
3QJ9X-ray2.30A/B32-579[»]
3QK5X-ray2.20A/B32-579[»]
3QKVX-ray3.10A/B32-579[»]
4DO3X-ray2.25A/B32-575[»]
4HBPX-ray2.91A/B30-579[»]
4J5PX-ray2.30A/B30-579[»]
ProteinModelPortaliP97612
SMRiP97612
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-46284N
STRINGi10116.ENSRNOP00000015961

Chemistry databases

BindingDBiP97612
ChEMBLiCHEMBL3229
GuidetoPHARMACOLOGYi1400
SwissLipidsiSLP:000001428

PTM databases

iPTMnetiP97612
PhosphoSitePlusiP97612

Proteomic databases

PaxDbiP97612
PRIDEiP97612

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000015667; ENSRNOP00000015667; ENSRNOG00000045949
ENSRNOT00000015961; ENSRNOP00000015961; ENSRNOG00000011019
GeneIDi100911581
29347
KEGGirno:100911581
rno:29347
UCSCiRGD:61808 rat

Organism-specific databases

CTDi2166
RGDi61808 Faah

Phylogenomic databases

eggNOGiKOG1212 Eukaryota
COG0154 LUCA
GeneTreeiENSGT00550000074673
HOGENOMiHOG000016500
HOVERGENiHBG005632
InParanoidiP97612
KOiK15528
OMAiTSMPGQE
OrthoDBiEOG091G05JU

Enzyme and pathway databases

BRENDAi3.5.1.4 5301
3.5.1.99 5301
ReactomeiR-RNO-2142753 Arachidonic acid metabolism

Miscellaneous databases

EvolutionaryTraceiP97612
PROiPR:P97612

Gene expression databases

BgeeiENSRNOG00000011019 Expressed in 8 organ(s), highest expression level in liver
GenevisibleiP97612 RN

Family and domain databases

Gene3Di3.90.1300.10, 1 hit
InterProiView protein in InterPro
IPR000120 Amidase
IPR020556 Amidase_CS
IPR023631 Amidase_dom
IPR036928 AS_sf
IPR030560 FAAH
PANTHERiPTHR11895 PTHR11895, 1 hit
PTHR11895:SF91 PTHR11895:SF91, 1 hit
PfamiView protein in Pfam
PF01425 Amidase, 1 hit
SUPFAMiSSF75304 SSF75304, 1 hit
PROSITEiView protein in PROSITE
PS00571 AMIDASES, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiFAAH1_RAT
AccessioniPrimary (citable) accession number: P97612
Secondary accession number(s): Q5BKA3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 1, 1997
Last modified: September 12, 2018
This is version 142 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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Main funding by: National Institutes of Health

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