We will be switching to the new UniProt website on Tuesday, June 28. Please explore and share your feedback.
Take me to UniProt BETA
UniProtKB - P97571 (CAN1_RAT)
Protein
Calpain-1 catalytic subunit
Gene
Capn1
Organism
Rattus norvegicus (Rat)
Status
Functioni
Calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. Proteolytically cleaves CTBP1 at 'Asn-364', 'Gly-377' and 'His-399'.
By similarityCatalytic activityi
- Broad endopeptidase specificity.By similarity EC:3.4.22.52
Cofactori
Ca2+By similarityNote: Binds 4 Ca2+ ions.By similarity
Activity regulationi
Activated by micromolar concentrations of calcium and inhibited by calpastatin.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 115 | By similarity | 1 | |
Active sitei | 272 | By similarity | 1 | |
Active sitei | 296 | By similarity | 1 | |
Metal bindingi | 597 | Calcium 1PROSITE-ProRule annotation | 1 | |
Metal bindingi | 599 | Calcium 1PROSITE-ProRule annotation | 1 | |
Metal bindingi | 601 | Calcium 1PROSITE-ProRule annotation | 1 | |
Metal bindingi | 603 | Calcium 1PROSITE-ProRule annotation | 1 | |
Metal bindingi | 608 | Calcium 1PROSITE-ProRule annotation | 1 | |
Metal bindingi | 627 | Calcium 2PROSITE-ProRule annotation | 1 | |
Metal bindingi | 629 | Calcium 2PROSITE-ProRule annotation | 1 | |
Metal bindingi | 631 | Calcium 2PROSITE-ProRule annotation | 1 | |
Metal bindingi | 633 | Calcium 2PROSITE-ProRule annotation | 1 | |
Metal bindingi | 638 | Calcium 2PROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- calcium-dependent cysteine-type endopeptidase activity Source: UniProtKB
- calcium ion binding Source: RGD
- cytoskeletal protein binding Source: RGD
- enzyme binding Source: RGD
- peptidase activity Source: RGD
GO - Biological processi
- aging Source: RGD
- brain development Source: RGD
- cellular response to hydrogen peroxide Source: RGD
- mammary gland involution Source: RGD
- negative regulation of actin filament polymerization Source: RGD
- negative regulation of NIK/NF-kappaB signaling Source: RGD
- positive regulation of cardiac muscle cell apoptotic process Source: RGD
- positive regulation of leukocyte tethering or rolling Source: RGD
- positive regulation of vascular permeability Source: RGD
- protein autoprocessing Source: RGD
- proteolysis Source: UniProtKB
- receptor catabolic process Source: RGD
- regulation of catalytic activity Source: UniProtKB
- response to angiotensin Source: RGD
- response to arsenic-containing substance Source: RGD
- self proteolysis Source: UniProtKB
Keywordsi
Molecular function | Hydrolase, Protease, Thiol protease |
Ligand | Calcium, Metal-binding |
Enzyme and pathway databases
BRENDAi | 3.4.22.52, 5301 |
Reactomei | R-RNO-1474228, Degradation of the extracellular matrix R-RNO-6798695, Neutrophil degranulation |
Protein family/group databases
MEROPSi | C02.001 |
Names & Taxonomyi
Protein namesi | Recommended name: Calpain-1 catalytic subunitCurated (EC:3.4.22.52By similarity)Alternative name(s): Calcium-activated neutral proteinase 1 Short name: CANP 1By similarity Calpain mu-typeBy similarity Calpain-1 large subunitBy similarity Micromolar-calpain Short name: muCANPBy similarity |
Gene namesi | |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 2267, Capn1 |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm By similarity
Plasma membrane
- Cell membrane By similarity
Note: Translocates to the plasma membrane upon Ca2+ binding.By similarity
Cytosol
- cytosol Source: RGD
Lysosome
- lysosome Source: RGD
Mitochondrion
- mitochondrion Source: RGD
Plasma Membrane
- cornified envelope Source: RGD
- plasma membrane Source: UniProtKB
Other locations
Keywords - Cellular componenti
Cell membrane, Cytoplasm, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000207700 | 1 – 713 | Calpain-1 catalytic subunitAdd BLAST | 713 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 354 | PhosphothreonineBy similarity | 1 |
Post-translational modificationi
Undergoes calcium-induced successive autoproteolytic cleavages that generate a membrane-bound 78 kDa active form and an intracellular 75 kDa active form. Calpastatin reduces with high efficiency the transition from 78 kDa to 75 kDa calpain forms (By similarity).By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 15 – 16 | Cleavage; for 78 kDa formBy similarity | 2 | |
Sitei | 27 – 28 | Cleavage; for 75 kDa formBy similarity | 2 |
Keywords - PTMi
Autocatalytic cleavage, PhosphoproteinProteomic databases
jPOSTi | P97571 |
PaxDbi | P97571 |
PTM databases
iPTMneti | P97571 |
PhosphoSitePlusi | P97571 |
Interactioni
Subunit structurei
Forms a heterodimer with a small (regulatory) subunit CAPNS1.
1 PublicationGO - Molecular functioni
- cytoskeletal protein binding Source: RGD
- enzyme binding Source: RGD
Protein-protein interaction databases
BioGRIDi | 247836, 3 interactors |
IntActi | P97571, 1 interactor |
STRINGi | 10116.ENSRNOP00000028431 |
Chemistry databases
BindingDBi | P97571 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
AlphaFoldDBi | P97571 |
SMRi | P97571 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P97571 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 55 – 354 | Calpain catalyticPROSITE-ProRule annotationAdd BLAST | 300 | |
Domaini | 557 – 575 | EF-hand 1PROSITE-ProRule annotationAdd BLAST | 19 | |
Domaini | 584 – 609 | EF-hand 2PROSITE-ProRule annotationAdd BLAST | 26 | |
Domaini | 614 – 649 | EF-hand 3PROSITE-ProRule annotationAdd BLAST | 36 | |
Domaini | 679 – 713 | EF-hand 4PROSITE-ProRule annotationAdd BLAST | 35 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 355 – 525 | Domain IIIAdd BLAST | 171 | |
Regioni | 526 – 541 | LinkerAdd BLAST | 16 | |
Regioni | 542 – 712 | Domain IVAdd BLAST | 171 |
Sequence similaritiesi
Belongs to the peptidase C2 family.Curated
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG0045, Eukaryota |
InParanoidi | P97571 |
PhylomeDBi | P97571 |
Family and domain databases
CDDi | cd00214, Calpain_III, 1 hit cd00044, CysPc, 1 hit |
InterProi | View protein in InterPro IPR033883, C2_III IPR022684, Calpain_cysteine_protease IPR022682, Calpain_domain_III IPR022683, Calpain_III IPR036213, Calpain_III_sf IPR029643, CAPN1 IPR011992, EF-hand-dom_pair IPR018247, EF_Hand_1_Ca_BS IPR002048, EF_hand_dom IPR038765, Papain-like_cys_pep_sf IPR000169, Pept_cys_AS IPR001300, Peptidase_C2_calpain_cat |
PANTHERi | PTHR10183:SF284, PTHR10183:SF284, 1 hit |
Pfami | View protein in Pfam PF01067, Calpain_III, 1 hit PF13833, EF-hand_8, 1 hit PF00648, Peptidase_C2, 1 hit |
PRINTSi | PR00704, CALPAIN |
SMARTi | View protein in SMART SM00720, calpain_III, 1 hit SM00230, CysPc, 1 hit |
SUPFAMi | SSF47473, SSF47473, 1 hit SSF49758, SSF49758, 1 hit SSF54001, SSF54001, 1 hit |
PROSITEi | View protein in PROSITE PS50203, CALPAIN_CAT, 1 hit PS00018, EF_HAND_1, 2 hits PS50222, EF_HAND_2, 4 hits PS00139, THIOL_PROTEASE_CYS, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
P97571-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAEELITPVY CTGVSAQVQK QRDKELGLGR HENAIKYLGQ DYENLRARCL
60 70 80 90 100
QNGVLFQDDA FPPVSHSLGF KELGPNSSKT YGIKWKRPTE LLSNPQFIVD
110 120 130 140 150
GATRTDICQG ALGDCWLLAA IASLTLNETI LHRVVPYGQS FQEGYAGIFH
160 170 180 190 200
FQLWQFGEWV DVVVDDLLPT KDGKLVFVHS AQGNEFWSAL LEKAYAKVNG
210 220 230 240 250
SYEALSGGCT SEAFEDFTGG VTEWYDLQKA PSDLYQIILK ALERGSLLGC
260 270 280 290 300
SINISDIRDL EAITFKNLVR GHAYSVTDAK QVTYQGQRVN LIRMRNPWGE
310 320 330 340 350
VEWKGPWSDN SYEWNKVDPY EREQLRVKME DGEFWMSFRD FIREFTKLEI
360 370 380 390 400
CNLTPDALKS RTLRNWNTTF YEGTWRRGST AGGCRNYPAT FWVNPQFKIR
410 420 430 440 450
LEEVDDADDY DSRESGCSFL LALMQKHRRR ERRFGRDMET IGFAVYQVPR
460 470 480 490 500
ELAGQPVHLK RDFFLANASR AQSEHFINLR EVSNRIRLPP GEYIVVPSTF
510 520 530 540 550
EPNKEGDFLL RFFSEKKAGT QELDDQIQAN LPDEKVLSEE EIDDNFKTLF
560 570 580 590 600
SKLAGDDMEI SVKELQTILN RIISKHKDLR TNGFSLESCR SMVNLMDRDG
610 620 630 640 650
NGKLGLVEFN ILWNRIRNYL TIFRKFDLDK SGSMSAYEMR MAIEAAGFKL
660 670 680 690 700
NKKLHELIIT RYSEPDLAVD FDNFVCCLVR LETMFRFFKI LDTDLDGVVT
710
FDLFKWLQLT MFA
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketF1LS29 | F1LS29_RAT | Calpain-1 catalytic subunit | Capn1 | 723 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U53858 mRNA Translation: AAC53001.1 BC061880 mRNA Translation: AAH61880.1 |
RefSeqi | NP_062025.1, NM_019152.2 |
Genome annotation databases
GeneIDi | 29153 |
KEGGi | rno:29153 |
UCSCi | RGD:2267, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U53858 mRNA Translation: AAC53001.1 BC061880 mRNA Translation: AAH61880.1 |
RefSeqi | NP_062025.1, NM_019152.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1KXR | X-ray | 2.07 | A/B | 27-356 | [»] | |
1QXP | X-ray | 2.80 | A/B | 60-647 | [»] | |
1TL9 | X-ray | 1.80 | A | 27-356 | [»] | |
1TLO | X-ray | 1.90 | A | 27-356 | [»] | |
2G8E | X-ray | 2.25 | A | 27-356 | [»] | |
2G8J | X-ray | 1.61 | A | 27-356 | [»] | |
2NQG | X-ray | 2.04 | A | 27-356 | [»] | |
2NQI | X-ray | 2.04 | A | 27-356 | [»] | |
2R9C | X-ray | 1.80 | A | 27-356 | [»] | |
2R9F | X-ray | 1.60 | A | 27-356 | [»] | |
AlphaFoldDBi | P97571 | |||||
SMRi | P97571 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 247836, 3 interactors |
IntActi | P97571, 1 interactor |
STRINGi | 10116.ENSRNOP00000028431 |
Chemistry databases
BindingDBi | P97571 |
ChEMBLi | CHEMBL3747 |
Protein family/group databases
MEROPSi | C02.001 |
PTM databases
iPTMneti | P97571 |
PhosphoSitePlusi | P97571 |
Proteomic databases
jPOSTi | P97571 |
PaxDbi | P97571 |
Genome annotation databases
GeneIDi | 29153 |
KEGGi | rno:29153 |
UCSCi | RGD:2267, rat |
Organism-specific databases
CTDi | 823 |
RGDi | 2267, Capn1 |
Phylogenomic databases
eggNOGi | KOG0045, Eukaryota |
InParanoidi | P97571 |
PhylomeDBi | P97571 |
Enzyme and pathway databases
BRENDAi | 3.4.22.52, 5301 |
Reactomei | R-RNO-1474228, Degradation of the extracellular matrix R-RNO-6798695, Neutrophil degranulation |
Miscellaneous databases
EvolutionaryTracei | P97571 |
PROi | PR:P97571 |
Family and domain databases
CDDi | cd00214, Calpain_III, 1 hit cd00044, CysPc, 1 hit |
InterProi | View protein in InterPro IPR033883, C2_III IPR022684, Calpain_cysteine_protease IPR022682, Calpain_domain_III IPR022683, Calpain_III IPR036213, Calpain_III_sf IPR029643, CAPN1 IPR011992, EF-hand-dom_pair IPR018247, EF_Hand_1_Ca_BS IPR002048, EF_hand_dom IPR038765, Papain-like_cys_pep_sf IPR000169, Pept_cys_AS IPR001300, Peptidase_C2_calpain_cat |
PANTHERi | PTHR10183:SF284, PTHR10183:SF284, 1 hit |
Pfami | View protein in Pfam PF01067, Calpain_III, 1 hit PF13833, EF-hand_8, 1 hit PF00648, Peptidase_C2, 1 hit |
PRINTSi | PR00704, CALPAIN |
SMARTi | View protein in SMART SM00720, calpain_III, 1 hit SM00230, CysPc, 1 hit |
SUPFAMi | SSF47473, SSF47473, 1 hit SSF49758, SSF49758, 1 hit SSF54001, SSF54001, 1 hit |
PROSITEi | View protein in PROSITE PS50203, CALPAIN_CAT, 1 hit PS00018, EF_HAND_1, 2 hits PS50222, EF_HAND_2, 4 hits PS00139, THIOL_PROTEASE_CYS, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | CAN1_RAT | |
Accessioni | P97571Primary (citable) accession number: P97571 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 18, 2001 |
Last sequence update: | May 1, 1997 | |
Last modified: | May 25, 2022 | |
This is version 181 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Peptidase families
Classification of peptidase families and list of entries - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families