UniProtKB - P97492 (RGS14_MOUSE)
Regulator of G-protein signaling 14
Rgs14
Functioni
GO - Molecular functioni
- GDP-dissociation inhibitor activity Source: UniProtKB
- G-protein alpha-subunit binding Source: MGI
- GTPase activating protein binding Source: UniProtKB
- GTPase activator activity Source: UniProtKB
- microtubule binding Source: UniProtKB
- protein kinase binding Source: MGI
- signaling receptor complex adaptor activity Source: UniProtKB
GO - Biological processi
- cell division Source: UniProtKB
- chromosome segregation Source: UniProtKB
- G protein-coupled receptor signaling pathway Source: MGI
- intracellular signal transduction Source: InterPro
- learning Source: UniProtKB
- long-term memory Source: UniProtKB
- long-term synaptic potentiation Source: UniProtKB
- mitotic cell cycle Source: MGI
- modulation of chemical synaptic transmission Source: SynGO
- negative regulation of ERK1 and ERK2 cascade Source: UniProtKB
- negative regulation of G protein-coupled receptor signaling pathway Source: UniProtKB
- negative regulation of MAP kinase activity Source: UniProtKB
- negative regulation of synaptic plasticity Source: UniProtKB
- nucleocytoplasmic transport Source: UniProtKB
- platelet-derived growth factor receptor signaling pathway Source: UniProtKB
- positive regulation of GTPase activity Source: UniProtKB
- positive regulation of neurogenesis Source: UniProtKB
- regulation of DNA-templated transcription in response to stress Source: UniProtKB
- regulation of G protein-coupled receptor signaling pathway Source: MGI
- response to oxidative stress Source: UniProtKB
- spindle organization Source: UniProtKB
- visual learning Source: UniProtKB
- zygote asymmetric cell division Source: UniProtKB
Keywordsi
Molecular function | Developmental protein, GTPase activation, Signal transduction inhibitor |
Biological process | Cell cycle, Cell division |
Enzyme and pathway databases
Reactomei | R-MMU-418594, G alpha (i) signalling events |
Names & Taxonomyi
Protein namesi | Recommended name: Regulator of G-protein signaling 14Short name: RGS14 Alternative name(s): RAP1/RAP2-interacting protein Short name: RPIP1 |
Gene namesi | Name:Rgs14 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1859709, Rgs14 |
Subcellular locationi
Cytoskeleton
- spindle
- spindle pole By similarity
- centrosome
Nucleus
Plasma membrane
- Cell membrane By similarity
Other locations
Note: Localizes with spindle poles during metaphase. Shuttles between the nucleus and cytoplasm in a CRM1-dependent manner. Recruited from the cytosol to the plasma membrane by the inactive GDP-bound forms of G(i) alpha subunits GNAI1 and GNAI3. Recruited from the cytosol to membranes by the active GTP-bound form of HRAS. Colocalizes with G(i) alpha subunit GNAI1 and RIC8A at the plasma membrane. Colocalizes with BRAF and RAF1 in both the cytoplasm and membranes (By similarity). Associates with the perinuclear sheaths of microtubules (MTs) surrounding the pronuclei, prior to segregating to the anastral mitotic apparatus and subsequently the barrel- shaped cytoplasmic bridge between the nascent nuclei of the emerging 2-cell embryo. Localizes to a perinuclear compartment near the microtubule-organizing center (MTOC). Expressed in the nucleus during interphase and segregates to the centrosomes and astral MTs during mitosis. Shuttles between the nucleus and cytoplasm in a CRM1-dependent manner. Relocalizes to the nucleus in PML nuclear bodies in respons to heat stress. Colocalizes with RIC8A in CA2 hippocampal neurons.By similarity
Cytoskeleton
- centrosome Source: UniProtKB
- microtubule Source: UniProtKB-KW
- spindle Source: UniProtKB
- spindle pole Source: UniProtKB
Nucleus
- nuclear body Source: UniProtKB
- nucleus Source: UniProtKB
- PML body Source: UniProtKB-SubCell
Plasma Membrane
- plasma membrane Source: UniProtKB
Other locations
- cytoplasm Source: UniProtKB
- dendrite Source: UniProtKB
- dendritic spine Source: UniProtKB
- glutamatergic synapse Source: SynGO
- postsynaptic density Source: UniProtKB
Keywords - Cellular componenti
Cell junction, Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, Membrane, Microtubule, Nucleus, SynapsePathology & Biotechi
Disruption phenotypei
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 92 – 93 | EN → AA: Inhibits GAP activity. Does not inhibit interaction with GNAI1 in the centrosomes. Reduces the down-regulation of G(i)-dependent signaling. Does not affect subcellular location and does not promote gene transcription activation. Inhibits strongly the down-regulation of G(i)-dependent signaling; when associated with F-519. Inhibits the interaction with GNAI1 in the centrosomes; when associated with A-518. 3 Publications | 2 | |
Mutagenesisi | 261 | S → A: Does not affect subcellular location; when associated with A-497. 1 Publication | 1 | |
Mutagenesisi | 336 | R → L: Reduces interaction with RABGEF1 and RAP2A. Strongly reduces interaction with RAP2A; when associated with L-409. 3 Publications | 1 | |
Mutagenesisi | 409 | H → L: Does not reduce interaction with RAP2A. Strongly reduces interaction with RAP2A; when associated with L-336. 2 Publications | 1 | |
Mutagenesisi | 497 | T → A: Does not affect subcellular location; when associated with A-261. 1 Publication | 1 | |
Mutagenesisi | 506 – 507 | LL → AA: Strongly expressed in the nucleus, mainly associated with PML nuclear bodies but not with centrosomes. Promotes gene transcription activation. 1 Publication | 2 | |
Mutagenesisi | 518 | Q → A: Inhibits GDI activity. Does not inhibit interaction with GNAI1 in the centrosomes, does not affect subcellular location and does not promote gene transcription activation. Inhibits interaction with GNAI1 in the centrosomes; when associated with A-92-93-A. 2 Publications | 1 | |
Mutagenesisi | 519 | R → F: Reduces interaction with GNAI1 and GNAI2. Inhibits strongly the down-regulation of G(i)-dependent signaling; when associated with A-92-93-A. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000204218 | 1 – 547 | Regulator of G-protein signaling 14Add BLAST | 547 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 20 | PhosphoserineBy similarity | 1 | |
Modified residuei | 42 | PhosphoserineBy similarity | 1 | |
Modified residuei | 45 | PhosphoserineBy similarity | 1 | |
Modified residuei | 143 | PhosphoserineBy similarity | 1 | |
Modified residuei | 199 | PhosphoserineCombined sources | 1 | |
Modified residuei | 203 | PhosphoserineBy similarity | 1 | |
Modified residuei | 218 | PhosphoserineBy similarity | 1 | |
Modified residuei | 249 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 289 | PhosphoserineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | P97492 |
jPOSTi | P97492 |
MaxQBi | P97492 |
PaxDbi | P97492 |
PeptideAtlasi | P97492 |
PRIDEi | P97492 |
PTM databases
iPTMneti | P97492 |
PhosphoSitePlusi | P97492 |
Expressioni
Tissue specificityi
Developmental stagei
Gene expression databases
Bgeei | ENSMUSG00000052087, Expressed in granulocyte and 214 other tissues |
Genevisiblei | P97492, MM |
Interactioni
Subunit structurei
Interacts with GNAI1 and GNAI2 (PubMed:15112653, PubMed:17635935).
Interacts with GNAI3 (By similarity).
Interacts with GNAO1 (PubMed:10926822).
Interacts (via RGS and GoLoco domains) with GNAI1; the interaction occurs in the centrosomes. Interaction with GNAI1 or GNAI3 (via active GTP- or inactive GDP-bound forms) prevents association of RGS14 with centrosomes or nuclear localization (By similarity).
Interacts with RABGEF1; the interactions is GTP-dependent (PubMed:10926822, PubMed:15112653).
Interacts with RAP2A; the interactions is GTP-dependent and does not alter its function on G(i) alpha subunits either as GAP or as GDI (PubMed:10926822, PubMed:15112653). Associates with microtubules (By similarity).
Found in a complex with at least BRAF, HRAS, MAP2K1, MAPK3 and RGS14.
Interacts with RIC8A (via C-terminus).
Interacts (via RBD 1 domain) with HRAS (active GTP-bound form preferentially).
Interacts (via RBD domains) with BRAF (via N-terminus); the interaction mediates the formation of a ternary complex with RAF1.
Interacts (via RBD domains) with RAF1 (via N-terminus); the interaction mediates the formation of a ternary complex with BRAF.
Interacts with KRAS (active GTP-bound form preferentially), MRAS (active GTP-bound form preferentially), NRAS (active GTP-bound form preferentially) and RRAS (active GTP-bound form preferentially) (By similarity).
By similarity3 PublicationsGO - Molecular functioni
- G-protein alpha-subunit binding Source: MGI
- GTPase activating protein binding Source: UniProtKB
- microtubule binding Source: UniProtKB
- protein kinase binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 206175, 230 interactors |
IntActi | P97492, 2 interactors |
MINTi | P97492 |
STRINGi | 10090.ENSMUSP00000068731 |
Miscellaneous databases
RNActi | P97492, protein |
Structurei
Secondary structure
3D structure databases
SMRi | P97492 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P97492 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 67 – 184 | RGSPROSITE-ProRule annotationAdd BLAST | 118 | |
Domaini | 303 – 374 | RBD 1PROSITE-ProRule annotationAdd BLAST | 72 | |
Domaini | 376 – 446 | RBD 2PROSITE-ProRule annotationAdd BLAST | 71 | |
Domaini | 500 – 522 | GoLocoPROSITE-ProRule annotationAdd BLAST | 23 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 300 – 427 | Necessary for interaction with RABGEF11 PublicationAdd BLAST | 128 |
Domaini
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG3589, Eukaryota |
GeneTreei | ENSGT00940000161364 |
HOGENOMi | CLU_024780_1_1_1 |
InParanoidi | P97492 |
OMAi | SCRANDQ |
OrthoDBi | 275783at2759 |
TreeFami | TF328814 |
Family and domain databases
CDDi | cd08743, RGS_RGS14, 1 hit |
Gene3Di | 1.10.196.10, 1 hit |
InterProi | View protein in InterPro IPR003109, GoLoco_motif IPR003116, RBD_dom IPR016137, RGS IPR030776, RGS14 IPR037881, RGS14_RGS IPR036305, RGS_sf IPR024066, RGS_subdom1/3 IPR029071, Ubiquitin-like_domsf |
PANTHERi | PTHR45945:SF2, PTHR45945:SF2, 1 hit |
Pfami | View protein in Pfam PF02188, GoLoco, 1 hit PF02196, RBD, 2 hits PF00615, RGS, 1 hit |
PRINTSi | PR01301, RGSPROTEIN |
SMARTi | View protein in SMART SM00390, GoLoco, 1 hit SM00455, RBD, 2 hits SM00315, RGS, 1 hit |
SUPFAMi | SSF48097, SSF48097, 1 hit SSF54236, SSF54236, 2 hits |
PROSITEi | View protein in PROSITE PS50877, GOLOCO, 1 hit PS50898, RBD, 2 hits PS50132, RGS, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
10 20 30 40 50
MPGKPKHLGV PNGRMVLAVS DGELTSTAGS QAQGEGRGSS LSIHSLPSGP
60 70 80 90 100
SSPFSTEEQP VASWAQSFER LLQDPRGLAY FTEFLKKEFS AENVTFWKAC
110 120 130 140 150
ERFQQIPASD TKQLAQEAHN IYHEFLSSQA LSPVNIDRQA WLSEEVLAQP
160 170 180 190 200
RPDMFRAQQL QIFNLMKFDS YARFVKSPLY QECLLAEAEG RPLREPGSSH
210 220 230 240 250
LGSPDTARKK PKLKPGKSLP LGVEELGQLP LAEGPCGRPL RKSFRREMTG
260 270 280 290 300
GAMNSALRRE SQGSLNSSAS LDLGFLAFVS SKSESHRKSL GSGESESESR
310 320 330 340 350
PGKYCCVYLP DGTASLALAR PGLTIRDMLA GICEKRGLSL PDIKVYLVGN
360 370 380 390 400
EQKALVLDQD CTVLADQEVR LENRITFQLE LVGLERVVRI SAKPTKRLQE
410 420 430 440 450
ALQPILAKHG LSLDQVVLHR PGEKQPMDLE NPVSSVASQT LVLDTPPDAK
460 470 480 490 500
MSEARSISPC RSQGCLPRTQ TKDSHLPPSS SSLLVEDASS STGNRQTCDI
510 520 530 540
EGLVELLNRV QSSGAHDQRG LLRKEDLVLP EFLQLPSQRP GSREAPP
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketD6RCH4 | D6RCH4_MOUSE | Regulator of G-protein-signaling 14 | Rgs14 | 82 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 209 | Missing in BAB22436 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 431 | N → T in AAB41893 (Ref. 1) Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U85055 mRNA Translation: AAB41893.1 CT009762 Genomic DNA No translation available. BC030321 mRNA Translation: AAH30321.1 AK002891 mRNA Translation: BAB22436.1 |
CCDSi | CCDS36674.1 |
RefSeqi | NP_058038.2, NM_016758.3 |
Genome annotation databases
Ensembli | ENSMUST00000063771; ENSMUSP00000068731; ENSMUSG00000052087 |
GeneIDi | 51791 |
KEGGi | mmu:51791 |
UCSCi | uc007qqq.2, mouse |
Similar proteinsi
Cross-referencesi
Web resourcesi
Protein Spotlight A balanced mind - Issue 132 of October 2011 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U85055 mRNA Translation: AAB41893.1 CT009762 Genomic DNA No translation available. BC030321 mRNA Translation: AAH30321.1 AK002891 mRNA Translation: BAB22436.1 |
CCDSi | CCDS36674.1 |
RefSeqi | NP_058038.2, NM_016758.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1WFY | NMR | - | A | 366-456 | [»] | |
SMRi | P97492 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 206175, 230 interactors |
IntActi | P97492, 2 interactors |
MINTi | P97492 |
STRINGi | 10090.ENSMUSP00000068731 |
PTM databases
iPTMneti | P97492 |
PhosphoSitePlusi | P97492 |
Proteomic databases
EPDi | P97492 |
jPOSTi | P97492 |
MaxQBi | P97492 |
PaxDbi | P97492 |
PeptideAtlasi | P97492 |
PRIDEi | P97492 |
Protocols and materials databases
ABCDi | P97492, 2 sequenced antibodies |
Antibodypediai | 29266, 315 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000063771; ENSMUSP00000068731; ENSMUSG00000052087 |
GeneIDi | 51791 |
KEGGi | mmu:51791 |
UCSCi | uc007qqq.2, mouse |
Organism-specific databases
CTDi | 10636 |
MGIi | MGI:1859709, Rgs14 |
Phylogenomic databases
eggNOGi | KOG3589, Eukaryota |
GeneTreei | ENSGT00940000161364 |
HOGENOMi | CLU_024780_1_1_1 |
InParanoidi | P97492 |
OMAi | SCRANDQ |
OrthoDBi | 275783at2759 |
TreeFami | TF328814 |
Enzyme and pathway databases
Reactomei | R-MMU-418594, G alpha (i) signalling events |
Miscellaneous databases
BioGRID-ORCSi | 51791, 3 hits in 19 CRISPR screens |
ChiTaRSi | Rgs14, mouse |
EvolutionaryTracei | P97492 |
PROi | PR:P97492 |
RNActi | P97492, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000052087, Expressed in granulocyte and 214 other tissues |
Genevisiblei | P97492, MM |
Family and domain databases
CDDi | cd08743, RGS_RGS14, 1 hit |
Gene3Di | 1.10.196.10, 1 hit |
InterProi | View protein in InterPro IPR003109, GoLoco_motif IPR003116, RBD_dom IPR016137, RGS IPR030776, RGS14 IPR037881, RGS14_RGS IPR036305, RGS_sf IPR024066, RGS_subdom1/3 IPR029071, Ubiquitin-like_domsf |
PANTHERi | PTHR45945:SF2, PTHR45945:SF2, 1 hit |
Pfami | View protein in Pfam PF02188, GoLoco, 1 hit PF02196, RBD, 2 hits PF00615, RGS, 1 hit |
PRINTSi | PR01301, RGSPROTEIN |
SMARTi | View protein in SMART SM00390, GoLoco, 1 hit SM00455, RBD, 2 hits SM00315, RGS, 1 hit |
SUPFAMi | SSF48097, SSF48097, 1 hit SSF54236, SSF54236, 2 hits |
PROSITEi | View protein in PROSITE PS50877, GOLOCO, 1 hit PS50898, RBD, 2 hits PS50132, RGS, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RGS14_MOUSE | |
Accessioni | P97492Primary (citable) accession number: P97492 Secondary accession number(s): Q8K2R4, Q9DCD1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 15, 1998 |
Last sequence update: | July 27, 2011 | |
Last modified: | December 2, 2020 | |
This is version 176 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - Protein Spotlight
Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries