UniProtKB - P97477 (AURKA_MOUSE)
Aurora kinase A
Aurka
Functioni
Mitotic serine/threonine kinase that contributes to the regulation of cell cycle progression. Associates with the centrosome and the spindle microtubules during mitosis and plays a critical role in various mitotic events including the establishment of mitotic spindle, centrosome duplication, centrosome separation as well as maturation, chromosomal alignment, spindle assembly checkpoint, and cytokinesis. Required for normal spindle positioning during mitosis and for the localization of NUMA1 and DCTN1 to the cell cortex during metaphase (By similarity).
Required for initial activation of CDK1 at centrosomes. Phosphorylates numerous target proteins, including ARHGEF2, BORA, BRCA1, CDC25B, DLGP5, HDAC6, KIF2A, LATS2, NDEL1, PARD3, PPP1R2, PLK1, RASSF1, TACC3, p53/TP53 and TPX2. Regulates KIF2A tubulin depolymerase activity. Required for normal axon formation. Plays a role in microtubule remodeling during neurite extension. Important for microtubule formation and/or stabilization. Also acts as a key regulatory component of the p53/TP53 pathway, and particularly the checkpoint-response pathways critical for oncogenic transformation of cells, by phosphorylating and destabilizing p53/TP53. Phosphorylates its own inhibitors, the protein phosphatase type 1 (PP1) isoforms, to inhibit their activity. Necessary for proper cilia disassembly prior to mitosis. Regulates protein levels of the anti-apoptosis protein BIRC5 by suppressing the expression of the SCF(FBXL7) E3 ubiquitin-protein ligase substrate adapter FBXL7 through the phosphorylation of the transcription factor FOXP1 (By similarity).
By similarity4 PublicationsMiscellaneous
Catalytic activityi
Activity regulationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 134 | ATP; via amide nitrogenPROSITE-ProRule annotation | 1 | |
Binding sitei | 153 | ATPPROSITE-ProRule annotation | 1 | |
Active sitei | 247 | Proton acceptorPROSITE-ProRule annotation | 1 | |
Binding sitei | 265 | ATPPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 201 – 204 | ATPPROSITE-ProRule annotation | 4 | |
Nucleotide bindingi | 251 – 252 | ATPBy similarity | 2 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- histone serine kinase activity Source: MGI
- protein heterodimerization activity Source: MGI
- protein kinase activity Source: MGI
- protein kinase binding Source: MGI
- protein serine/threonine/tyrosine kinase activity Source: RHEA
- protein serine/threonine kinase activity Source: MGI
- protein serine kinase activity Source: RHEA
- ubiquitin protein ligase binding Source: MGI
GO - Biological processi
- anterior/posterior axis specification Source: MGI
- cell division Source: UniProtKB-KW
- centrosome cycle Source: MGI
- centrosome localization Source: MGI
- liver regeneration Source: MGI
- meiotic cell cycle Source: MGI
- meiotic spindle organization Source: MGI
- microtubule cytoskeleton organization Source: MGI
- mitotic cell cycle Source: MGI
- mitotic centrosome separation Source: MGI
- mitotic spindle organization Source: MGI
- negative regulation of apoptotic process Source: MGI
- negative regulation of gene expression Source: MGI
- negative regulation of protein binding Source: MGI
- neuron projection extension Source: MGI
- peptidyl-serine phosphorylation Source: MGI
- positive regulation of mitochondrial fission Source: MGI
- positive regulation of oocyte maturation Source: MGI
- positive regulation of proteasomal ubiquitin-dependent protein catabolic process Source: MGI
- protein localization to centrosome Source: MGI
- protein phosphorylation Source: MGI
- regulation of cytokinesis Source: GO_Central
- regulation of protein stability Source: MGI
- response to wounding Source: MGI
- spindle assembly involved in female meiosis I Source: MGI
- spindle organization Source: MGI
Keywordsi
Molecular function | Kinase, Serine/threonine-protein kinase, Transferase |
Biological process | Cell cycle, Cell division, Cilium biogenesis/degradation, Mitosis |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-MMU-174178, APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1 R-MMU-2565942, Regulation of PLK1 Activity at G2/M Transition R-MMU-6804114, TP53 Regulates Transcription of Genes Involved in G2 Cell Cycle Arrest R-MMU-6804756, Regulation of TP53 Activity through Phosphorylation R-MMU-8854050, FBXL7 down-regulates AURKA during mitotic entry and in early mitosis R-MMU-8854518, AURKA Activation by TPX2 R-MMU-8854521, Interaction between PHLDA1 and AURKA |
Names & Taxonomyi
Protein namesi | Recommended name: Aurora kinase A (EC:2.7.11.1By similarity)Alternative name(s): Aurora 2 Aurora family kinase 1 Aurora/IPL1-related kinase 1 Short name: ARK-1 Short name: Aurora-related kinase 1 Ipl1- and aurora-related kinase 1 Serine/threonine-protein kinase 6 Serine/threonine-protein kinase Ayk1 Serine/threonine-protein kinase aurora-A |
Gene namesi | Name:Aurka Synonyms:Aik, Airk, Ark1, Aura, Ayk1, Btak, Iak1, Stk15, Stk6 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:894678, Aurka |
VEuPathDBi | HostDB:ENSMUSG00000027496 |
Subcellular locationi
Cytoskeleton
- centrosome 2 Publications
- spindle pole 2 Publications
- cilium basal body 1 Publication
- centriole 1 Publication
Other locations
- neuron projection 1 Publication
Note: Localizes on centrosomes in interphase cells and at each spindle pole in mitosis (PubMed:9245792). Associates with both the pericentriolar material (PCM) and centrioles (By similarity). Colocalized with SIRT2 at centrosome (By similarity). Detected at the neurite hillock in developing neurons (PubMed:19668197). The localization to the spindle poles is regulated by AAAS (By similarity).By similarity2 Publications
Cytoskeleton
- centriole Source: UniProtKB-SubCell
- centrosome Source: MGI
- chromosome passenger complex Source: GO_Central
- meiotic spindle Source: MGI
- microtubule cytoskeleton Source: MGI
- microtubule organizing center Source: MGI
- mitotic spindle Source: MGI
- mitotic spindle pole Source: UniProtKB
- spindle Source: MGI
- spindle microtubule Source: GO_Central
- spindle midzone Source: GO_Central
- spindle pole centrosome Source: MGI
Cytosol
- cytosol Source: MGI
Nucleus
- germinal vesicle Source: MGI
- nucleoplasm Source: MGI
- nucleus Source: MGI
- pronucleus Source: MGI
Other locations
- axon hillock Source: MGI
- cilium Source: UniProtKB-KW
- perinuclear region of cytoplasm Source: MGI
Keywords - Cellular componenti
Cell projection, Cilium, Cytoplasm, Cytoskeleton, MicrotubulePathology & Biotechi
Disruption phenotypei
Keywords - Diseasei
Proto-oncogeneChemistry databases
ChEMBLi | CHEMBL2211 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000086693 | 1 – 395 | Aurora kinase AAdd BLAST | 395 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 40 | PhosphoserineBy similarity | 1 | |
Modified residuei | 50 | PhosphoserineBy similarity | 1 | |
Cross-linki | 249 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 278 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 279 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 333 | Phosphoserine; by PKA and PAKBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | P97477 |
MaxQBi | P97477 |
PaxDbi | P97477 |
PeptideAtlasi | P97477 |
PRIDEi | P97477 |
ProteomicsDBi | 277137 [P97477-1] 277138 [P97477-2] |
PTM databases
iPTMneti | P97477 |
PhosphoSitePlusi | P97477 |
Expressioni
Tissue specificityi
Developmental stagei
Inductioni
Gene expression databases
Bgeei | ENSMUSG00000027496, Expressed in secondary oocyte and 240 other tissues |
ExpressionAtlasi | P97477, baseline and differential |
Genevisiblei | P97477, MM |
Interactioni
Subunit structurei
Interacts with CPEB1, JTB, TACC1, TPX2, PPP2CA, as well as with the protein phosphatase type 1 (PP1) isoforms PPP1CA, PPP1CB and PPP1CC (By similarity).
Interacts also with its substrates ARHGEF2, BORA, KIF2A, PARD3, and p53/TP53 (By similarity). Interaction with BORA promotes phosphorylation of PLK1 (By similarity).
Interacts with GADD45A, competing with its oligomerization (By similarity).
Interacts with FBXL7 and PIFO (PubMed:20643351, PubMed:22306998).
Interacts (via C-terminus) with AUNIP (via C-terminus) (By similarity).
Identified in a complex with AUNIP and NIN (By similarity).
Interacts with SIRT2 (By similarity).
Interacts with FRY; this interaction facilitates AURKA-mediated PLK1 phosphorylation (PubMed:22753416).
Interacts with MYCN; interaction is phospho-independent and triggers AURKA activation; AURKA competes with FBXW7 for binding to unphosphorylated MYCN but not for binding to phosphorylated MYCN (By similarity).
Interacts with HNRNPU (By similarity).
Interacts with AAAS (By similarity).
Interacts with KLHL18 and CUL3 (By similarity).
Interacts with FOXP1 (By similarity).
By similarity5 PublicationsGO - Molecular functioni
- protein heterodimerization activity Source: MGI
- protein kinase binding Source: MGI
- ubiquitin protein ligase binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 203548, 12 interactors |
IntActi | P97477, 1 interactor |
STRINGi | 10090.ENSMUSP00000028997 |
Chemistry databases
BindingDBi | P97477 |
Miscellaneous databases
RNActi | P97477, protein |
Structurei
Secondary structure
3D structure databases
SMRi | P97477 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P97477 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 124 – 374 | Protein kinasePROSITE-ProRule annotationAdd BLAST | 251 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 114 | DisorderedSequence analysisAdd BLAST | 114 | |
Regioni | 271 – 284 | Activation segmentBy similarityAdd BLAST | 14 | |
Regioni | 376 – 395 | DisorderedSequence analysisAdd BLAST | 20 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 27 – 60 | Polar residuesSequence analysisAdd BLAST | 34 | |
Compositional biasi | 82 – 104 | Polar residuesSequence analysisAdd BLAST | 23 | |
Compositional biasi | 379 – 395 | Polar residuesSequence analysisAdd BLAST | 17 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0580, Eukaryota |
GeneTreei | ENSGT00940000154900 |
HOGENOMi | CLU_000288_63_6_1 |
InParanoidi | P97477 |
OMAi | ERMSVND |
PhylomeDBi | P97477 |
TreeFami | TF105331 |
Family and domain databases
InterProi | View protein in InterPro IPR030616, Aur IPR011009, Kinase-like_dom_sf IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR008271, Ser/Thr_kinase_AS |
PANTHERi | PTHR24350, PTHR24350, 1 hit |
Pfami | View protein in Pfam PF00069, Pkinase, 1 hit |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MDRCKENCVS RPVKTTVPFG PKRVLVTEQI PSQNLGSASS GQAQRVLCPS
60 70 80 90 100
NSQRVPSQAQ KLGAGQKPAP KQLPAASVPR PVSRLNNPQK NEQPAASGND
110 120 130 140 150
SEKEQASLQK TEDTKKRQWT LEDFDIGRPL GKGKFGNVYL ARERQSKFIL
160 170 180 190 200
ALKVLFKTQL EKANVEHQLR REVEIQSHLR HPNILRLYGY FHDATRVYLI
210 220 230 240 250
LEYAPLGTVY RELQKLSKFD EQRTATYITE LANALSYCHS KRVIHRDIKP
260 270 280 290 300
ENLLLGSNGE LKIADFGWSV HAPSSRRTTM CGTLDYLPPE MIEGRMHDEK
310 320 330 340 350
VDLWSLGVLC YEFLVGMPPF EAHTYQETYR RISRVEFTFP DFVTEGARDL
360 370 380 390
ISRLLKHNAS QRLTLAEVLE HPWIKANSSK PPTGHTSKEP TSKSS
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 234 | A → T in AAB63205 (PubMed:9245792).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_004871 | 1 | M → MAVEGEPGCCKRIGKAVWRR GDM in isoform 2. 2 Publications | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U80932 mRNA Translation: AAB62982.1 AF007817 mRNA Translation: AAB63205.1 U69106 mRNA Translation: AAC12682.1 AK085861 mRNA Translation: BAC39557.1 Frameshift. BC005425 mRNA Translation: AAH05425.1 BC014711 mRNA Translation: AAH14711.1 |
CCDSi | CCDS17129.1 [P97477-2] CCDS71204.1 [P97477-1] |
PIRi | JC5975 |
RefSeqi | NP_001278114.1, NM_001291185.1 [P97477-1] NP_035627.1, NM_011497.4 [P97477-2] XP_006499138.1, XM_006499075.2 [P97477-1] |
Genome annotation databases
Ensembli | ENSMUST00000028997; ENSMUSP00000028997; ENSMUSG00000027496 [P97477-2] ENSMUST00000109139; ENSMUSP00000104767; ENSMUSG00000027496 [P97477-1] ENSMUST00000109140; ENSMUSP00000104768; ENSMUSG00000027496 [P97477-1] |
GeneIDi | 20878 |
KEGGi | mmu:20878 |
UCSCi | uc008ocn.2, mouse [P97477-2] uc008oco.2, mouse [P97477-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U80932 mRNA Translation: AAB62982.1 AF007817 mRNA Translation: AAB63205.1 U69106 mRNA Translation: AAC12682.1 AK085861 mRNA Translation: BAC39557.1 Frameshift. BC005425 mRNA Translation: AAH05425.1 BC014711 mRNA Translation: AAH14711.1 |
CCDSi | CCDS17129.1 [P97477-2] CCDS71204.1 [P97477-1] |
PIRi | JC5975 |
RefSeqi | NP_001278114.1, NM_001291185.1 [P97477-1] NP_035627.1, NM_011497.4 [P97477-2] XP_006499138.1, XM_006499075.2 [P97477-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3D14 | X-ray | 1.90 | A | 116-381 | [»] | |
3D15 | X-ray | 2.30 | A | 116-381 | [»] | |
3D2I | X-ray | 2.90 | A | 116-381 | [»] | |
3D2K | X-ray | 2.50 | A | 116-381 | [»] | |
3DAJ | X-ray | 2.00 | A | 116-381 | [»] | |
3DJ5 | X-ray | 1.80 | A | 116-381 | [»] | |
3DJ6 | X-ray | 1.70 | A | 116-381 | [»] | |
3DJ7 | X-ray | 2.80 | A | 116-381 | [»] | |
SMRi | P97477 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 203548, 12 interactors |
IntActi | P97477, 1 interactor |
STRINGi | 10090.ENSMUSP00000028997 |
Chemistry databases
BindingDBi | P97477 |
ChEMBLi | CHEMBL2211 |
PTM databases
iPTMneti | P97477 |
PhosphoSitePlusi | P97477 |
Proteomic databases
EPDi | P97477 |
MaxQBi | P97477 |
PaxDbi | P97477 |
PeptideAtlasi | P97477 |
PRIDEi | P97477 |
ProteomicsDBi | 277137 [P97477-1] 277138 [P97477-2] |
Protocols and materials databases
Antibodypediai | 1129, 1065 antibodies from 46 providers |
DNASUi | 20878 |
Genome annotation databases
Ensembli | ENSMUST00000028997; ENSMUSP00000028997; ENSMUSG00000027496 [P97477-2] ENSMUST00000109139; ENSMUSP00000104767; ENSMUSG00000027496 [P97477-1] ENSMUST00000109140; ENSMUSP00000104768; ENSMUSG00000027496 [P97477-1] |
GeneIDi | 20878 |
KEGGi | mmu:20878 |
UCSCi | uc008ocn.2, mouse [P97477-2] uc008oco.2, mouse [P97477-1] |
Organism-specific databases
CTDi | 6790 |
MGIi | MGI:894678, Aurka |
VEuPathDBi | HostDB:ENSMUSG00000027496 |
Phylogenomic databases
eggNOGi | KOG0580, Eukaryota |
GeneTreei | ENSGT00940000154900 |
HOGENOMi | CLU_000288_63_6_1 |
InParanoidi | P97477 |
OMAi | ERMSVND |
PhylomeDBi | P97477 |
TreeFami | TF105331 |
Enzyme and pathway databases
Reactomei | R-MMU-174178, APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1 R-MMU-2565942, Regulation of PLK1 Activity at G2/M Transition R-MMU-6804114, TP53 Regulates Transcription of Genes Involved in G2 Cell Cycle Arrest R-MMU-6804756, Regulation of TP53 Activity through Phosphorylation R-MMU-8854050, FBXL7 down-regulates AURKA during mitotic entry and in early mitosis R-MMU-8854518, AURKA Activation by TPX2 R-MMU-8854521, Interaction between PHLDA1 and AURKA |
Miscellaneous databases
BioGRID-ORCSi | 20878, 21 hits in 68 CRISPR screens |
ChiTaRSi | Aurka, mouse |
EvolutionaryTracei | P97477 |
PROi | PR:P97477 |
RNActi | P97477, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000027496, Expressed in secondary oocyte and 240 other tissues |
ExpressionAtlasi | P97477, baseline and differential |
Genevisiblei | P97477, MM |
Family and domain databases
InterProi | View protein in InterPro IPR030616, Aur IPR011009, Kinase-like_dom_sf IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR008271, Ser/Thr_kinase_AS |
PANTHERi | PTHR24350, PTHR24350, 1 hit |
Pfami | View protein in Pfam PF00069, Pkinase, 1 hit |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | AURKA_MOUSE | |
Accessioni | P97477Primary (citable) accession number: P97477 Secondary accession number(s): O35624, Q8C3H8, Q91YU4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 27, 2003 |
Last sequence update: | May 1, 1997 | |
Last modified: | February 23, 2022 | |
This is version 193 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human and mouse protein kinases
Human and mouse protein kinases: classification and index - MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families