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Protein

RISC-loading complex subunit TARBP2

Gene

Tarbp2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Required for formation of the RNA induced silencing complex (RISC). Component of the RISC loading complex (RLC), also known as the micro-RNA (miRNA) loading complex (miRLC), which is composed of DICER1, AGO2 and TARBP2. Within the RLC/miRLC, DICER1 and TARBP2 are required to process precursor miRNAs (pre-miRNAs) to mature miRNAs and then load them onto AGO2. AGO2 bound to the mature miRNA constitutes the minimal RISC and may subsequently dissociate from DICER1 and TARBP2. May also play a role in the production of short interfering RNAs (siRNAs) from double-stranded RNA (dsRNA) by DICER1 (By similarity). Binds in vitro to the PRM1 3'-UTR. Seems to act as a repressor of translation (PubMed:8649414).UniRule annotation1 Publication

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionRNA-binding
Biological processRNA-mediated gene silencing, Translation regulation

Enzyme and pathway databases

ReactomeiR-MMU-203927 MicroRNA (miRNA) biogenesis
R-MMU-426486 Small interfering RNA (siRNA) biogenesis

Names & Taxonomyi

Protein namesi
Recommended name:
RISC-loading complex subunit TARBP2UniRule annotation
Alternative name(s):
Protamine-1 RNA-binding protein
Short name:
PRM-1 RNA-binding protein
TAR RNA-binding protein 2
Gene namesi
Name:Tarbp2
Synonyms:Prbp
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:103027 Tarbp2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000656231 – 365RISC-loading complex subunit TARBP2Add BLAST365

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei151PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiP97473
MaxQBiP97473
PaxDbiP97473
PeptideAtlasiP97473
PRIDEiP97473

PTM databases

iPTMnetiP97473
PhosphoSitePlusiP97473

Expressioni

Gene expression databases

BgeeiENSMUSG00000023051 Expressed in 32 organ(s), highest expression level in testis
CleanExiMM_TARBP2
ExpressionAtlasiP97473 baseline and differential
GenevisibleiP97473 MM

Interactioni

Subunit structurei

Self-associates. Component of the RISC loading complex (RLC), or micro-RNA (miRNA) loading complex (miRLC), which is composed of DICER1, AGO2 and TARBP2. Note that the trimeric RLC/miRLC is also referred to as RISC. Interacts with EIF2AK2/PKR and inhibits its protein kinase activity. Interacts with DHX9 (By similarity). Interacts with DICER1 and PRKRA (PubMed:16142218, PubMed:17452327). Interacts with DICER1, AGO2, MOV10, EIF6 and RPL7A (60S ribosome subunit); they form a large RNA-induced silencing complex (RISC) (By similarity).UniRule annotation2 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi203969, 1 interactor
ComplexPortaliCPX-135 RISC-loading complex, TARBP2 variant
IntActiP97473, 3 interactors
STRINGi10090.ENSMUSP00000023813

Structurei

3D structure databases

ProteinModelPortaliP97473
SMRiP97473
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini30 – 97DRBM 1UniRule annotationAdd BLAST68
Domaini158 – 226DRBM 2UniRule annotationAdd BLAST69
Domaini292 – 360DRBM 3UniRule annotationAdd BLAST69

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni22 – 105Sufficient for interaction with PRKRAUniRule annotationAdd BLAST84
Regioni151 – 233Sufficient for interaction with PRKRAUniRule annotationAdd BLAST83
Regioni227 – 365Sufficient for interaction with DICER1UniRule annotationAdd BLAST139
Regioni286 – 365Sufficient for interaction with PRKRAUniRule annotationAdd BLAST80

Sequence similaritiesi

Belongs to the TARBP2 family.UniRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG3732 Eukaryota
ENOG410XSCK LUCA
GeneTreeiENSGT00900000141030
HOGENOMiHOG000231919
HOVERGENiHBG001700
InParanoidiP97473
KOiK18420
OMAiQPITVCH
OrthoDBiEOG091G0I2L
TreeFamiTF315953

Family and domain databases

CDDicd00048 DSRM, 2 hits
HAMAPiMF_03034 TRBP2, 1 hit
InterProiView protein in InterPro
IPR014720 dsRBD_dom
IPR028605 TRBP2
PANTHERiPTHR10910:SF61 PTHR10910:SF61, 1 hit
PfamiView protein in Pfam
PF00035 dsrm, 2 hits
SMARTiView protein in SMART
SM00358 DSRM, 3 hits
PROSITEiView protein in PROSITE
PS50137 DS_RBD, 3 hits

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 8 potential isoforms that are computationally mapped.Show allAlign All

P97473-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSEEDQGSGT TTGCGLPSIE QMLAANPGKT PISLLQEYGT RIGKTPVYDL
60 70 80 90 100
LKAEGQAHQP NFTFRVTVGD TSCTGQGPSK KAAKHKAAEV ALKHLKGGSM
110 120 130 140 150
LEPALEDSSS FSLLDSSPPE DTPVVAAEAA APVPSAVLTR SPPMEMQPPV
160 170 180 190 200
SPQQSECNPV GALQELVVQK GWRLPEYMVT QESGPAHRKE FTMTCRVERF
210 220 230 240 250
IEIGSGTSKK LAKRNAAAKM LLRVHTVPLD ARDGNEAEPD DDHFSIGVSS
260 270 280 290 300
RLDGLRNRGP GCTWDSLRNS VGEKILSLRS CSVGSLGALG SACCSVLSEL
310 320 330 340 350
SEEQAFHVSY LDIEELSLSG LCQCLVELST QPATVCYGSA TTREAARGDA
360
AHRALQYLRI MAGSK
Length:365
Mass (Da):38,842
Last modified:July 27, 2011 - v2
Checksum:i220A1D10D71CC249
GO

Computationally mapped potential isoform sequencesi

There are 8 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
Q3UNH9Q3UNH9_MOUSE
RISC-loading complex subunit TARBP2
Tarbp2
274Annotation score:
D3YZS5D3YZS5_MOUSE
RISC-loading complex subunit TARBP2
Tarbp2
243Annotation score:
D3Z282D3Z282_MOUSE
RISC-loading complex subunit TARBP2
Tarbp2
225Annotation score:
D6RCY4D6RCY4_MOUSE
RISC-loading complex subunit TARBP2
Tarbp2
58Annotation score:
D6RE49D6RE49_MOUSE
RISC-loading complex subunit TARBP2
Tarbp2
79Annotation score:
D3Z033D3Z033_MOUSE
RISC-loading complex subunit TARBP2
Tarbp2
89Annotation score:
A0A2R8VHA7A0A2R8VHA7_MOUSE
RISC-loading complex subunit TARBP2
Tarbp2
111Annotation score:
A0A2R8VI89A0A2R8VI89_MOUSE
RISC-loading complex subunit TARBP2
Tarbp2
42Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti53A → E in AAB38885 (PubMed:8649414).Curated1
Sequence conflicti76Q → T in AAB38885 (PubMed:8649414).Curated1
Sequence conflicti111F → L in AAB38885 (PubMed:8649414).Curated1
Sequence conflicti258R → H in AAB38885 (PubMed:8649414).Curated1
Sequence conflicti334T → A in AAB38885 (PubMed:8649414).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U79962 mRNA Translation: AAB38885.1
CH466550 Genomic DNA Translation: EDL03967.1
BC002028 mRNA Translation: AAH02028.1
CCDSiCCDS27886.1
RefSeqiNP_033345.2, NM_009319.3
UniGeneiMm.291485

Genome annotation databases

EnsembliENSMUST00000023813; ENSMUSP00000023813; ENSMUSG00000023051
GeneIDi21357
KEGGimmu:21357
UCSCiuc007xwf.3 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U79962 mRNA Translation: AAB38885.1
CH466550 Genomic DNA Translation: EDL03967.1
BC002028 mRNA Translation: AAH02028.1
CCDSiCCDS27886.1
RefSeqiNP_033345.2, NM_009319.3
UniGeneiMm.291485

3D structure databases

ProteinModelPortaliP97473
SMRiP97473
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi203969, 1 interactor
ComplexPortaliCPX-135 RISC-loading complex, TARBP2 variant
IntActiP97473, 3 interactors
STRINGi10090.ENSMUSP00000023813

PTM databases

iPTMnetiP97473
PhosphoSitePlusiP97473

Proteomic databases

EPDiP97473
MaxQBiP97473
PaxDbiP97473
PeptideAtlasiP97473
PRIDEiP97473

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000023813; ENSMUSP00000023813; ENSMUSG00000023051
GeneIDi21357
KEGGimmu:21357
UCSCiuc007xwf.3 mouse

Organism-specific databases

CTDi6895
MGIiMGI:103027 Tarbp2

Phylogenomic databases

eggNOGiKOG3732 Eukaryota
ENOG410XSCK LUCA
GeneTreeiENSGT00900000141030
HOGENOMiHOG000231919
HOVERGENiHBG001700
InParanoidiP97473
KOiK18420
OMAiQPITVCH
OrthoDBiEOG091G0I2L
TreeFamiTF315953

Enzyme and pathway databases

ReactomeiR-MMU-203927 MicroRNA (miRNA) biogenesis
R-MMU-426486 Small interfering RNA (siRNA) biogenesis

Miscellaneous databases

PROiPR:P97473
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000023051 Expressed in 32 organ(s), highest expression level in testis
CleanExiMM_TARBP2
ExpressionAtlasiP97473 baseline and differential
GenevisibleiP97473 MM

Family and domain databases

CDDicd00048 DSRM, 2 hits
HAMAPiMF_03034 TRBP2, 1 hit
InterProiView protein in InterPro
IPR014720 dsRBD_dom
IPR028605 TRBP2
PANTHERiPTHR10910:SF61 PTHR10910:SF61, 1 hit
PfamiView protein in Pfam
PF00035 dsrm, 2 hits
SMARTiView protein in SMART
SM00358 DSRM, 3 hits
PROSITEiView protein in PROSITE
PS50137 DS_RBD, 3 hits
ProtoNetiSearch...

Entry informationi

Entry nameiTRBP2_MOUSE
AccessioniPrimary (citable) accession number: P97473
Secondary accession number(s): Q99M41
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: July 27, 2011
Last modified: November 7, 2018
This is version 142 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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