UniProtKB - P97468 (CML1_MOUSE)
Protein
Chemokine-like receptor 1
Gene
Cmklr1
Organism
Mus musculus (Mouse)
Status
Functioni
Receptor for the chemoattractant adipokine chemerin/RARRES2 and for the omega-3 fatty acid derived molecule resolvin E1. Interaction with RARRES2 induces activation of intracellular signaling molecules, such as SKY, MAPK1/3 (ERK1/2), MAPK14/P38MAPK and PI3K leading to multifunctional effects, like reduction of immune responses, enhancing of adipogenesis and angionesis. Resolvin E1 down-regulates cytokine production in macrophages by reducing the activation of MAPK1/3 (ERK1/2) and NF-kappa-B (By similarity). Positively regulates adipogenesis and adipocyte metabolism.By similarity3 Publications
GO - Molecular functioni
- complement receptor activity Source: GO_Central
- G protein-coupled receptor activity Source: GO_Central
GO - Biological processi
- chemotaxis Source: MGI
- complement receptor mediated signaling pathway Source: GO_Central
- G protein-coupled receptor signaling pathway Source: MGI
- inflammatory response Source: GO_Central
- negative regulation of interleukin-12 production Source: UniProtKB
- negative regulation of NF-kappaB transcription factor activity Source: UniProtKB
- phospholipase C-activating G protein-coupled receptor signaling pathway Source: GO_Central
- positive regulation of cold-induced thermogenesis Source: YuBioLab
- positive regulation of cytosolic calcium ion concentration Source: GO_Central
- positive regulation of fat cell differentiation Source: UniProtKB
- positive regulation of macrophage chemotaxis Source: MGI
- regulation of calcium-mediated signaling Source: MGI
Keywordsi
Molecular function | G-protein coupled receptor, Receptor, Transducer |
Enzyme and pathway databases
Reactomei | R-MMU-373076, Class A/1 (Rhodopsin-like receptors) |
Names & Taxonomyi
Protein namesi | Recommended name: Chemokine-like receptor 1Alternative name(s): G-protein coupled receptor DEZ |
Gene namesi | Name:Cmklr1 Synonyms:Dez, Gpcr27 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:109603, Cmklr1 |
Subcellular locationi
Plasma membrane
- Cell membrane 1 Publication; Multi-pass membrane protein 1 Publication
Plasma Membrane
- plasma membrane Source: MGI
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 39 | ExtracellularSequence analysisAdd BLAST | 39 | |
Transmembranei | 40 – 62 | Helical; Name=1Sequence analysisAdd BLAST | 23 | |
Topological domaini | 63 – 73 | CytoplasmicSequence analysisAdd BLAST | 11 | |
Transmembranei | 74 – 95 | Helical; Name=2Sequence analysisAdd BLAST | 22 | |
Topological domaini | 96 – 112 | ExtracellularSequence analysisAdd BLAST | 17 | |
Transmembranei | 113 – 133 | Helical; Name=3Sequence analysisAdd BLAST | 21 | |
Topological domaini | 134 – 152 | CytoplasmicSequence analysisAdd BLAST | 19 | |
Transmembranei | 153 – 174 | Helical; Name=4Sequence analysisAdd BLAST | 22 | |
Topological domaini | 175 – 222 | ExtracellularSequence analysisAdd BLAST | 48 | |
Transmembranei | 223 – 243 | Helical; Name=5Sequence analysisAdd BLAST | 21 | |
Topological domaini | 244 – 259 | CytoplasmicSequence analysisAdd BLAST | 16 | |
Transmembranei | 260 – 280 | Helical; Name=6Sequence analysisAdd BLAST | 21 | |
Topological domaini | 281 – 298 | ExtracellularSequence analysisAdd BLAST | 18 | |
Transmembranei | 299 – 318 | Helical; Name=7Sequence analysisAdd BLAST | 20 | |
Topological domaini | 319 – 371 | CytoplasmicSequence analysisAdd BLAST | 53 |
Keywords - Cellular componenti
Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000069308 | 1 – 371 | Chemokine-like receptor 1Add BLAST | 371 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 7 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 110 ↔ 187 | PROSITE-ProRule annotation | ||
Glycosylationi | 190 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Modified residuei | 337 | PhosphoserineCombined sources | 1 | |
Modified residuei | 340 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 347 | PhosphoserineCombined sources | 1 | |
Modified residuei | 350 | PhosphoserineCombined sources | 1 | |
Modified residuei | 356 | PhosphoserineBy similarity | 1 | |
Modified residuei | 370 | PhosphothreonineCombined sources | 1 |
Keywords - PTMi
Disulfide bond, Glycoprotein, PhosphoproteinProteomic databases
jPOSTi | P97468 |
PaxDbi | P97468 |
PRIDEi | P97468 |
PTM databases
GlyGeni | P97468, 2 sites |
iPTMneti | P97468 |
PhosphoSitePlusi | P97468 |
Expressioni
Tissue specificityi
Expressed in the differentiated adipocytes (at protein level). Ubiquitous. Highly expressed in adipose tissue and immature plasmacytoid dendritic cells (DCs) and at lower levels in myeloid DCs, macrophages, and NK cells. Expressed on macrophages isolated from different tissues, including peritoneal cavities, pleural cavities and spleen.5 Publications
Developmental stagei
Expressed during bone development and in adult parathyroid glands. Expressed at 11 dpc in the caudal part of the tongue and the umbilical cord and the expression in the tongue was maintained throughout adulthood. Expression increases in bone and cartilaginous forming zones of embryo up to stage 14.5 dpc and at 16.5 dpc expression is seen in the lung.1 Publication
Inductioni
Down-regulated by bacterial lipopolysaccharide (LPS), IFN-alpha and TNF on macrophages.1 Publication
Gene expression databases
Bgeei | ENSMUSG00000042190, Expressed in secondary oocyte and 174 other tissues |
Genevisiblei | P97468, MM |
Interactioni
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000036316 |
Miscellaneous databases
RNActi | P97468, protein |
Family & Domainsi
Sequence similaritiesi
Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG3656, Eukaryota |
GeneTreei | ENSGT01010000222468 |
HOGENOMi | CLU_009579_8_0_1 |
InParanoidi | P97468 |
OMAi | NIAYTAM |
OrthoDBi | 910274at2759 |
PhylomeDBi | P97468 |
TreeFami | TF330976 |
Family and domain databases
InterProi | View protein in InterPro IPR002258, Chemokine-like_recpt_1 IPR000826, Formyl_rcpt-rel IPR000276, GPCR_Rhodpsn IPR017452, GPCR_Rhodpsn_7TM |
PANTHERi | PTHR24225, PTHR24225, 1 hit PTHR24225:SF49, PTHR24225:SF49, 1 hit |
Pfami | View protein in Pfam PF00001, 7tm_1, 1 hit |
PRINTSi | PR01126, DEZORPHANR PR00237, GPCRRHODOPSN |
PROSITEi | View protein in PROSITE PS00237, G_PROTEIN_RECEP_F1_1, 1 hit PS50262, G_PROTEIN_RECEP_F1_2, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
P97468-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MEYDAYNDSG IYDDEYSDGF GYFVDLEEAS PWEAKVAPVF LVVIYSLVCF
60 70 80 90 100
LGLLGNGLVI VIATFKMKKT VNTVWFVNLA VADFLFNIFL PMHITYAAMD
110 120 130 140 150
YHWVFGKAMC KISNFLLSHN MYTSVFLLTV ISFDRCISVL LPVWSQNHRS
160 170 180 190 200
IRLAYMTCSA VWVLAFFLSS PSLVFRDTAN IHGKITCFNN FSLAAPESSP
210 220 230 240 250
HPAHSQVVST GYSRHVAVTV TRFLCGFLIP VFIITACYLT IVFKLQRNRL
260 270 280 290 300
AKNKKPFKII ITIIITFFLC WCPYHTLYLL ELHHTAVPSS VFSLGLPLAT
310 320 330 340 350
AVAIANSCMN PILYVFMGHD FRKFKVALFS RLANALSEDT GPSSYPSHRS
360 370
FTKMSSLNEK ASVNEKETST L
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketE9Q8N2 | E9Q8N2_MOUSE | Chemokine-like receptor 1 | Cmklr1 | 301 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U79525 mRNA Translation: AAB53789.1 |
CCDSi | CCDS19551.1 |
PIRi | JC5498 |
RefSeqi | NP_032179.1, NM_008153.3 XP_006530227.1, XM_006530164.3 XP_006530228.1, XM_006530165.2 XP_006530229.1, XM_006530166.2 |
Genome annotation databases
Ensembli | ENSMUST00000047936; ENSMUSP00000036316; ENSMUSG00000042190 |
GeneIDi | 14747 |
KEGGi | mmu:14747 |
UCSCi | uc012ebo.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U79525 mRNA Translation: AAB53789.1 |
CCDSi | CCDS19551.1 |
PIRi | JC5498 |
RefSeqi | NP_032179.1, NM_008153.3 XP_006530227.1, XM_006530164.3 XP_006530228.1, XM_006530165.2 XP_006530229.1, XM_006530166.2 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000036316 |
Protein family/group databases
GPCRDBi | Search... |
PTM databases
GlyGeni | P97468, 2 sites |
iPTMneti | P97468 |
PhosphoSitePlusi | P97468 |
Proteomic databases
jPOSTi | P97468 |
PaxDbi | P97468 |
PRIDEi | P97468 |
Protocols and materials databases
Antibodypediai | 9299, 563 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000047936; ENSMUSP00000036316; ENSMUSG00000042190 |
GeneIDi | 14747 |
KEGGi | mmu:14747 |
UCSCi | uc012ebo.1, mouse |
Organism-specific databases
CTDi | 1240 |
MGIi | MGI:109603, Cmklr1 |
Phylogenomic databases
eggNOGi | KOG3656, Eukaryota |
GeneTreei | ENSGT01010000222468 |
HOGENOMi | CLU_009579_8_0_1 |
InParanoidi | P97468 |
OMAi | NIAYTAM |
OrthoDBi | 910274at2759 |
PhylomeDBi | P97468 |
TreeFami | TF330976 |
Enzyme and pathway databases
Reactomei | R-MMU-373076, Class A/1 (Rhodopsin-like receptors) |
Miscellaneous databases
BioGRID-ORCSi | 14747, 0 hits in 19 CRISPR screens |
ChiTaRSi | Cmklr1, mouse |
PROi | PR:P97468 |
RNActi | P97468, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000042190, Expressed in secondary oocyte and 174 other tissues |
Genevisiblei | P97468, MM |
Family and domain databases
InterProi | View protein in InterPro IPR002258, Chemokine-like_recpt_1 IPR000826, Formyl_rcpt-rel IPR000276, GPCR_Rhodpsn IPR017452, GPCR_Rhodpsn_7TM |
PANTHERi | PTHR24225, PTHR24225, 1 hit PTHR24225:SF49, PTHR24225:SF49, 1 hit |
Pfami | View protein in Pfam PF00001, 7tm_1, 1 hit |
PRINTSi | PR01126, DEZORPHANR PR00237, GPCRRHODOPSN |
PROSITEi | View protein in PROSITE PS00237, G_PROTEIN_RECEP_F1_1, 1 hit PS50262, G_PROTEIN_RECEP_F1_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CML1_MOUSE | |
Accessioni | P97468Primary (citable) accession number: P97468 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 15, 1998 |
Last sequence update: | May 1, 1997 | |
Last modified: | December 2, 2020 | |
This is version 156 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- 7-transmembrane G-linked receptors
List of 7-transmembrane G-linked receptor entries - MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families