Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Docking protein 1

Gene

Dok1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK1 appears to be a negative regulator of the insulin signaling pathway. Modulates integrin activation by competing with talin for the same binding site on ITGB3 (By similarity).By similarity

GO - Biological processi

Enzyme and pathway databases

ReactomeiR-MMU-8849469 PTK6 Regulates RTKs and Their Effectors AKT1 and DOK1
R-MMU-8853659 RET signaling

Names & Taxonomyi

Protein namesi
Recommended name:
Docking protein 1
Alternative name(s):
Downstream of tyrosine kinase 1
p62(dok)
Gene namesi
Name:Dok1
Synonyms:Dok
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:893587 Dok1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

No visible phenotype. Mice appear healthy and are fertile.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001872691 – 482Docking protein 1Add BLAST482

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei48PhosphoserineBy similarity1
Modified residuei269PhosphoserineCombined sources1
Modified residuei290PhosphoserineBy similarity1
Modified residuei295PhosphotyrosineCombined sources1
Modified residuei336PhosphotyrosineCombined sources1
Modified residuei340PhosphotyrosineCombined sources1
Modified residuei361PhosphotyrosineCombined sources1
Modified residuei376PhosphotyrosineCombined sources1
Modified residuei397Phosphotyrosine; by INSRBy similarity1
Modified residuei408PhosphotyrosineCombined sources1
Modified residuei415PhosphoserineCombined sources1
Modified residuei450PhosphotyrosineCombined sources1

Post-translational modificationi

Constitutively tyrosine-phosphorylated. Phosphorylated by TEC. Phosphorylated on tyrosine residues by the insulin receptor kinase. Results in the negative regulation of the insulin signaling pathway (By similarity). Phosphorylated by LYN. Phosphorylated on tyrosine residues by SRMS (By similarity).By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiP97465
MaxQBiP97465
PaxDbiP97465
PeptideAtlasiP97465
PRIDEiP97465

PTM databases

iPTMnetiP97465
PhosphoSitePlusiP97465

Expressioni

Tissue specificityi

Expressed in lung, spleen, skeletal muscle and kidney.

Gene expression databases

BgeeiENSMUSG00000068335 Expressed in 183 organ(s), highest expression level in bone marrow macrophage
CleanExiMM_DOK1
ExpressionAtlasiP97465 baseline and differential
GenevisibleiP97465 MM

Interactioni

Subunit structurei

Interacts with RasGAP, INPP5D/SHIP1 and ABL1. Interacts directly with phosphorylated ITGB3 (By similarity). Interacts with SRMS (via the SH2 and SH3 domains) (By similarity).By similarity

Binary interactionsi

Protein-protein interaction databases

BioGridi199267, 28 interactors
CORUMiP97465
IntActiP97465, 13 interactors
MINTiP97465
STRINGi10090.ENSMUSP00000087079

Structurei

Secondary structure

1482
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP97465
SMRiP97465
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP97465

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini4 – 119PHAdd BLAST116
Domaini151 – 259IRS-type PTBPROSITE-ProRule annotationAdd BLAST109

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi280 – 322Pro-richAdd BLAST43
Compositional biasi359 – 430Pro-richAdd BLAST72

Domaini

PTB domain mediates receptor interaction.

Sequence similaritiesi

Belongs to the DOK family. Type A subfamily.Curated

Phylogenomic databases

eggNOGiKOG4047 Eukaryota
ENOG410XS2S LUCA
GeneTreeiENSGT00730000110348
HOGENOMiHOG000112245
HOVERGENiHBG018962
InParanoidiP97465
KOiK14752
OMAiRADSHEG
OrthoDBiEOG091G075T
PhylomeDBiP97465
TreeFamiTF324994

Family and domain databases

CDDicd01203 PTB_DOK1_DOK2_DOK3, 1 hit
Gene3Di2.30.29.30, 2 hits
InterProiView protein in InterPro
IPR037751 Dok1/2/3_PTB
IPR002404 IRS_PTB
IPR011993 PH-like_dom_sf
IPR001849 PH_domain
PfamiView protein in Pfam
PF02174 IRS, 1 hit
PF00169 PH, 1 hit
SMARTiView protein in SMART
SM00233 PH, 1 hit
SM00310 PTBI, 1 hit
PROSITEiView protein in PROSITE
PS51064 IRS_PTB, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All

P97465-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MDGAVMEGPL FLQSQRFGTK RWRKTWAVLY PASPHGVARL EFFDHKGSSS
60 70 80 90 100
RGGRGGSRRL DCKMIRLAEC VSVVPVTVES PPEPGAVAFR LDTAQRSHLL
110 120 130 140 150
AADAVSSTAW VQTLCRTAFP KGGWALAQTE NQPKFSALEM LENSLYSPTW
160 170 180 190 200
EGSQFWVTSQ KTEASERCGL QGSYILRVEA EKLTLLTLGA QSQILEPLLF
210 220 230 240 250
WPYTLLRRYG RDKVMFSFEA GRRCPSGPGT FTFQTSQGND IFQAVEAAIQ
260 270 280 290 300
QQKAQGKVGQ AQDILRTDSH DGETEGKTVP PPVPQDPLGS PPALYAEPLD
310 320 330 340 350
SLRIPPGPSQ DSVYSDPLGS TPAGAGEGVH SKKPLYWDLY GHVQQQLLKT
360 370 380 390 400
KLTDSKEDPI YDEPEGLAPA PPRGLYDLPQ EPRDAWWCQA RLKEEGYELP
410 420 430 440 450
YNPATDDYAV PPPRSPKPAP APKPQGLILP ESGTTRGSGS KGFSSDTALY
460 470 480
SQVQKSGTSG AWDCGLSKVG NDRAGVKSEG ST
Length:482
Mass (Da):52,452
Last modified:January 17, 2003 - v2
Checksum:iC999C9FE0DA58EA3
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A0N4SW29A0A0N4SW29_MOUSE
Docking protein 1
Dok1
51Annotation score:
A0A0N4SW01A0A0N4SW01_MOUSE
Docking protein 1
Dok1
42Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti2D → N in AAH13066 (PubMed:15489334).Curated1
Sequence conflicti87V → A in AAC95339 (PubMed:9927484).Curated1
Sequence conflicti87V → A in AAH13066 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U78818 mRNA Translation: AAB48827.2
AF084363 Genomic DNA Translation: AAC95339.1
BC013066 mRNA Translation: AAH13066.1
CCDSiCCDS20265.1
RefSeqiNP_001278728.1, NM_001291799.1
NP_034200.4, NM_010070.4
UniGeneiMm.156

Genome annotation databases

EnsembliENSMUST00000089651; ENSMUSP00000087079; ENSMUSG00000068335
GeneIDi13448
KEGGimmu:13448
UCSCiuc009clr.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U78818 mRNA Translation: AAB48827.2
AF084363 Genomic DNA Translation: AAC95339.1
BC013066 mRNA Translation: AAH13066.1
CCDSiCCDS20265.1
RefSeqiNP_001278728.1, NM_001291799.1
NP_034200.4, NM_010070.4
UniGeneiMm.156

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1P5TX-ray2.35A/B152-266[»]
1UEFX-ray2.50A/B152-266[»]
ProteinModelPortaliP97465
SMRiP97465
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi199267, 28 interactors
CORUMiP97465
IntActiP97465, 13 interactors
MINTiP97465
STRINGi10090.ENSMUSP00000087079

PTM databases

iPTMnetiP97465
PhosphoSitePlusiP97465

Proteomic databases

EPDiP97465
MaxQBiP97465
PaxDbiP97465
PeptideAtlasiP97465
PRIDEiP97465

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000089651; ENSMUSP00000087079; ENSMUSG00000068335
GeneIDi13448
KEGGimmu:13448
UCSCiuc009clr.2 mouse

Organism-specific databases

CTDi1796
MGIiMGI:893587 Dok1

Phylogenomic databases

eggNOGiKOG4047 Eukaryota
ENOG410XS2S LUCA
GeneTreeiENSGT00730000110348
HOGENOMiHOG000112245
HOVERGENiHBG018962
InParanoidiP97465
KOiK14752
OMAiRADSHEG
OrthoDBiEOG091G075T
PhylomeDBiP97465
TreeFamiTF324994

Enzyme and pathway databases

ReactomeiR-MMU-8849469 PTK6 Regulates RTKs and Their Effectors AKT1 and DOK1
R-MMU-8853659 RET signaling

Miscellaneous databases

ChiTaRSiDok1 mouse
EvolutionaryTraceiP97465
PROiPR:P97465
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000068335 Expressed in 183 organ(s), highest expression level in bone marrow macrophage
CleanExiMM_DOK1
ExpressionAtlasiP97465 baseline and differential
GenevisibleiP97465 MM

Family and domain databases

CDDicd01203 PTB_DOK1_DOK2_DOK3, 1 hit
Gene3Di2.30.29.30, 2 hits
InterProiView protein in InterPro
IPR037751 Dok1/2/3_PTB
IPR002404 IRS_PTB
IPR011993 PH-like_dom_sf
IPR001849 PH_domain
PfamiView protein in Pfam
PF02174 IRS, 1 hit
PF00169 PH, 1 hit
SMARTiView protein in SMART
SM00233 PH, 1 hit
SM00310 PTBI, 1 hit
PROSITEiView protein in PROSITE
PS51064 IRS_PTB, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiDOK1_MOUSE
AccessioniPrimary (citable) accession number: P97465
Secondary accession number(s): Q9R213
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 16, 2001
Last sequence update: January 17, 2003
Last modified: November 7, 2018
This is version 165 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again