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Protein

Neuronal PAS domain-containing protein 1

Gene

Npas1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

May control regulatory pathways relevant to schizophrenia and to psychotic illness. May play a role in late central nervous system development by modulating EPO expression in response to cellular oxygen level. Forms a heterodimer that binds core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) leading to transcriptional repression on its target gene TH (PubMed:27782878).3 Publications

GO - Molecular functioni

  • DNA binding Source: UniProtKB-KW
  • DNA-binding transcription factor activity, RNA polymerase II-specific Source: GO_Central
  • protein heterodimerization activity Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionDNA-binding
Biological processTranscription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Neuronal PAS domain-containing protein 1
Short name:
Neuronal PAS1
Gene namesi
Name:Npas1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:109205 Npas1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi249F → D: Destabilizes heterodimerization with ARNT. Compromises the transcriptional repression activity of heterodimer ARNT:NPAS1. 1 Publication1
Mutagenesisi251R → A: Destabilizes heterodimerization with ARNT. Compromises the transcriptional repression activity of heterodimer ARNT:NPAS1. 1 Publication1
Mutagenesisi270V → D: Destabilizes heterodimerization with ARNT. Compromises the transcriptional repression activity of heterodimer ARNT:NPAS1. 1 Publication1
Mutagenesisi272H → A: Destabilizes heterodimerization with ARNT. Compromises the transcriptional repression activity of heterodimer ARNT:NPAS1. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001274051 – 594Neuronal PAS domain-containing protein 1Add BLAST594

Proteomic databases

PaxDbiP97459
PRIDEiP97459

PTM databases

PhosphoSitePlusiP97459

Expressioni

Tissue specificityi

Expressed in brain in inhibitory interneurons. Also found in spinal cord.1 Publication

Developmental stagei

First detected between embryonic day 15 and day 16.

Gene expression databases

BgeeiENSMUSG00000001988 Expressed in 58 organ(s), highest expression level in head
CleanExiMM_NPAS1
ExpressionAtlasiP97459 baseline and differential
GenevisibleiP97459 MM

Interactioni

Subunit structurei

Efficient DNA binding requires dimerization with another bHLH protein. Interacts with ARNT; forms a heterodimer that binds core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) leading to a transcriptional repressor on its target gene TH.2 Publications

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000002053

Structurei

Secondary structure

1594
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP97459
SMRiP97459
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini45 – 98bHLHPROSITE-ProRule annotationAdd BLAST54
Domaini135 – 205PAS 1PROSITE-ProRule annotationAdd BLAST71
Domaini294 – 360PAS 2PROSITE-ProRule annotationAdd BLAST67
Domaini366 – 409PACAdd BLAST44

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi212 – 223Poly-SerAdd BLAST12

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG3558 Eukaryota
ENOG410YK57 LUCA
GeneTreeiENSGT00760000118788
HOGENOMiHOG000236298
HOVERGENiHBG052655
InParanoidiP97459
KOiK09098
OMAiVPWDFLP
OrthoDBiEOG091G02J4
PhylomeDBiP97459
TreeFamiTF317772

Family and domain databases

CDDicd00083 HLH, 1 hit
cd00130 PAS, 2 hits
Gene3Di4.10.280.10, 1 hit
InterProiView protein in InterPro
IPR011598 bHLH_dom
IPR036638 HLH_DNA-bd_sf
IPR000014 PAS
IPR035965 PAS-like_dom_sf
IPR013767 PAS_fold
IPR013655 PAS_fold_3
PfamiView protein in Pfam
PF00010 HLH, 1 hit
PF00989 PAS, 1 hit
PF08447 PAS_3, 1 hit
SMARTiView protein in SMART
SM00353 HLH, 1 hit
SM00091 PAS, 2 hits
SUPFAMiSSF47459 SSF47459, 1 hit
SSF55785 SSF55785, 2 hits
TIGRFAMsiTIGR00229 sensory_box, 1 hit
PROSITEiView protein in PROSITE
PS50888 BHLH, 1 hit
PS50112 PAS, 1 hit

Sequencei

Sequence statusi: Complete.

P97459-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MATPYPRSGG RGEVKCGGGR GAGVPWDFLP GLMVKAPPGP CLQAQRKEKS
60 70 80 90 100
RNAARWRRGK ENLEFFELAK LLPLPGAISS QLDKASIVRL SVTYLRLRRF
110 120 130 140 150
AALGAPPWGL RAVGPPAGLA PGRRGPVALV SEVFEQHLGG HILQSLDGFV
160 170 180 190 200
FALNQEGKFL YISETVSIYL GLSQVELTGS SVFDYIHPGD HSEVLEQLGL
210 220 230 240 250
RAASIGPPTP PSVSSSSSSS SSSLVDTPEI EASPTEASPA FRAQERSFFV
260 270 280 290 300
RMKSTLTKRG LNVKASGYKV IHVTGRLRAR ALGLVALGHT LPPAPLAELP
310 320 330 340 350
LHGHMIVFRL SLGLTILACE SRVSDHMDMG PSELVGRSCY QFVHGQDATR
360 370 380 390 400
IRQSHLDLLD KGQVVTGYYR WLQRAGGFVW LQSVATVAGN GKSTGEHHVL
410 420 430 440 450
WVSHVLSNAE GSQTPLDAFQ LPAIVSQEEP SRPGPEPTEE EPPVDGKQAV
460 470 480 490 500
PADQDKDKDP QARGKRIKVE ASPKEARGSE DSGEEELSDP PAPPRPEFTS
510 520 530 540 550
VIRAGALKHD PVLPWGLTTP GDPSPALLHA GFLPPVVRGL CTPGTIRYGP
560 570 580 590
AELSLMYPHL HRLGAGPSLP EAFYPTLGLP YPGPTGTRVQ RKGD
Length:594
Mass (Da):63,737
Last modified:May 1, 1997 - v1
Checksum:iD6477696DF69A4B3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U77967 mRNA Translation: AAB47247.1
CCDSiCCDS20850.1
RefSeqiNP_032744.1, NM_008718.2
XP_017177510.1, XM_017322021.1
UniGeneiMm.145821

Genome annotation databases

EnsembliENSMUST00000002053; ENSMUSP00000002053; ENSMUSG00000001988
ENSMUST00000210748; ENSMUSP00000147412; ENSMUSG00000001988
GeneIDi18142
KEGGimmu:18142
UCSCiuc009fhv.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U77967 mRNA Translation: AAB47247.1
CCDSiCCDS20850.1
RefSeqiNP_032744.1, NM_008718.2
XP_017177510.1, XM_017322021.1
UniGeneiMm.145821

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5SY5X-ray3.20B/D/F43-423[»]
ProteinModelPortaliP97459
SMRiP97459
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000002053

PTM databases

PhosphoSitePlusiP97459

Proteomic databases

PaxDbiP97459
PRIDEiP97459

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000002053; ENSMUSP00000002053; ENSMUSG00000001988
ENSMUST00000210748; ENSMUSP00000147412; ENSMUSG00000001988
GeneIDi18142
KEGGimmu:18142
UCSCiuc009fhv.2 mouse

Organism-specific databases

CTDi4861
MGIiMGI:109205 Npas1

Phylogenomic databases

eggNOGiKOG3558 Eukaryota
ENOG410YK57 LUCA
GeneTreeiENSGT00760000118788
HOGENOMiHOG000236298
HOVERGENiHBG052655
InParanoidiP97459
KOiK09098
OMAiVPWDFLP
OrthoDBiEOG091G02J4
PhylomeDBiP97459
TreeFamiTF317772

Miscellaneous databases

PROiPR:P97459
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000001988 Expressed in 58 organ(s), highest expression level in head
CleanExiMM_NPAS1
ExpressionAtlasiP97459 baseline and differential
GenevisibleiP97459 MM

Family and domain databases

CDDicd00083 HLH, 1 hit
cd00130 PAS, 2 hits
Gene3Di4.10.280.10, 1 hit
InterProiView protein in InterPro
IPR011598 bHLH_dom
IPR036638 HLH_DNA-bd_sf
IPR000014 PAS
IPR035965 PAS-like_dom_sf
IPR013767 PAS_fold
IPR013655 PAS_fold_3
PfamiView protein in Pfam
PF00010 HLH, 1 hit
PF00989 PAS, 1 hit
PF08447 PAS_3, 1 hit
SMARTiView protein in SMART
SM00353 HLH, 1 hit
SM00091 PAS, 2 hits
SUPFAMiSSF47459 SSF47459, 1 hit
SSF55785 SSF55785, 2 hits
TIGRFAMsiTIGR00229 sensory_box, 1 hit
PROSITEiView protein in PROSITE
PS50888 BHLH, 1 hit
PS50112 PAS, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiNPAS1_MOUSE
AccessioniPrimary (citable) accession number: P97459
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: May 1, 1997
Last modified: November 7, 2018
This is version 145 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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