UniProtKB - P97459 (NPAS1_MOUSE)
Neuronal PAS domain-containing protein 1
Npas1
Functioni
May control regulatory pathways relevant to schizophrenia and to psychotic illness. May play a role in late central nervous system development by modulating EPO expression in response to cellular oxygen level. Forms a heterodimer that binds core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) leading to transcriptional repression on its target gene TH (PubMed:27782878).
3 PublicationsGO - Molecular functioni
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: GO_Central
- protein heterodimerization activity Source: UniProtKB
- RNA polymerase II transcription regulatory region sequence-specific DNA binding Source: GO_Central
GO - Biological processi
- maternal behavior Source: MGI
- negative regulation of transcription, DNA-templated Source: UniProtKB
- negative regulation of transcription by RNA polymerase II Source: MGI
- regulation of transcription by RNA polymerase II Source: GO_Central
- startle response Source: MGI
Keywordsi
Molecular function | DNA-binding |
Biological process | Transcription, Transcription regulation |
Names & Taxonomyi
Protein namesi | Recommended name: Neuronal PAS domain-containing protein 1Short name: Neuronal PAS1 |
Gene namesi | Name:Npas1 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:109205, Npas1 |
VEuPathDBi | HostDB:ENSMUSG00000001988 |
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 249 | F → D: Destabilizes heterodimerization with ARNT. Compromises the transcriptional repression activity of heterodimer ARNT:NPAS1. 1 Publication | 1 | |
Mutagenesisi | 251 | R → A: Destabilizes heterodimerization with ARNT. Compromises the transcriptional repression activity of heterodimer ARNT:NPAS1. 1 Publication | 1 | |
Mutagenesisi | 270 | V → D: Destabilizes heterodimerization with ARNT. Compromises the transcriptional repression activity of heterodimer ARNT:NPAS1. 1 Publication | 1 | |
Mutagenesisi | 272 | H → A: Destabilizes heterodimerization with ARNT. Compromises the transcriptional repression activity of heterodimer ARNT:NPAS1. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000127405 | 1 – 594 | Neuronal PAS domain-containing protein 1Add BLAST | 594 |
Proteomic databases
PaxDbi | P97459 |
PRIDEi | P97459 |
ProteomicsDBi | 253003 |
PTM databases
PhosphoSitePlusi | P97459 |
Expressioni
Tissue specificityi
Developmental stagei
Gene expression databases
Bgeei | ENSMUSG00000001988, Expressed in head and 98 other tissues |
Genevisiblei | P97459, MM |
Interactioni
Subunit structurei
Efficient DNA binding requires dimerization with another bHLH protein.
Interacts with ARNT; forms a heterodimer that binds core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) leading to a transcriptional repressor on its target gene TH.
2 PublicationsGO - Molecular functioni
- protein heterodimerization activity Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 201818, 1 interactor |
STRINGi | 10090.ENSMUSP00000002053 |
Miscellaneous databases
RNActi | P97459, protein |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | P97459 |
SMRi | P97459 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 45 – 98 | bHLHPROSITE-ProRule annotationAdd BLAST | 54 | |
Domaini | 135 – 205 | PAS 1PROSITE-ProRule annotationAdd BLAST | 71 | |
Domaini | 294 – 360 | PAS 2PROSITE-ProRule annotationAdd BLAST | 67 | |
Domaini | 366 – 409 | PACAdd BLAST | 44 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 206 – 237 | DisorderedSequence analysisAdd BLAST | 32 | |
Regioni | 427 – 498 | DisorderedSequence analysisAdd BLAST | 72 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 208 – 229 | Polar residuesSequence analysisAdd BLAST | 22 | |
Compositional biasi | 450 – 491 | Basic and acidic residuesSequence analysisAdd BLAST | 42 |
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG3558, Eukaryota |
GeneTreei | ENSGT00940000161295 |
HOGENOMi | CLU_010044_6_3_1 |
InParanoidi | P97459 |
OMAi | VCQTKNM |
OrthoDBi | 922461at2759 |
PhylomeDBi | P97459 |
TreeFami | TF317772 |
Family and domain databases
CDDi | cd00130, PAS, 2 hits |
Gene3Di | 4.10.280.10, 1 hit |
InterProi | View protein in InterPro IPR011598, bHLH_dom IPR036638, HLH_DNA-bd_sf IPR000014, PAS IPR035965, PAS-like_dom_sf IPR013767, PAS_fold IPR013655, PAS_fold_3 |
Pfami | View protein in Pfam PF00010, HLH, 1 hit PF00989, PAS, 1 hit PF08447, PAS_3, 1 hit |
SMARTi | View protein in SMART SM00353, HLH, 1 hit SM00091, PAS, 2 hits |
SUPFAMi | SSF47459, SSF47459, 1 hit SSF55785, SSF55785, 2 hits |
TIGRFAMsi | TIGR00229, sensory_box, 1 hit |
PROSITEi | View protein in PROSITE PS50888, BHLH, 1 hit PS50112, PAS, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MATPYPRSGG RGEVKCGGGR GAGVPWDFLP GLMVKAPPGP CLQAQRKEKS
60 70 80 90 100
RNAARWRRGK ENLEFFELAK LLPLPGAISS QLDKASIVRL SVTYLRLRRF
110 120 130 140 150
AALGAPPWGL RAVGPPAGLA PGRRGPVALV SEVFEQHLGG HILQSLDGFV
160 170 180 190 200
FALNQEGKFL YISETVSIYL GLSQVELTGS SVFDYIHPGD HSEVLEQLGL
210 220 230 240 250
RAASIGPPTP PSVSSSSSSS SSSLVDTPEI EASPTEASPA FRAQERSFFV
260 270 280 290 300
RMKSTLTKRG LNVKASGYKV IHVTGRLRAR ALGLVALGHT LPPAPLAELP
310 320 330 340 350
LHGHMIVFRL SLGLTILACE SRVSDHMDMG PSELVGRSCY QFVHGQDATR
360 370 380 390 400
IRQSHLDLLD KGQVVTGYYR WLQRAGGFVW LQSVATVAGN GKSTGEHHVL
410 420 430 440 450
WVSHVLSNAE GSQTPLDAFQ LPAIVSQEEP SRPGPEPTEE EPPVDGKQAV
460 470 480 490 500
PADQDKDKDP QARGKRIKVE ASPKEARGSE DSGEEELSDP PAPPRPEFTS
510 520 530 540 550
VIRAGALKHD PVLPWGLTTP GDPSPALLHA GFLPPVVRGL CTPGTIRYGP
560 570 580 590
AELSLMYPHL HRLGAGPSLP EAFYPTLGLP YPGPTGTRVQ RKGD
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U77967 mRNA Translation: AAB47247.1 |
CCDSi | CCDS20850.1 |
RefSeqi | NP_032744.1, NM_008718.2 XP_017177510.1, XM_017322021.1 |
Genome annotation databases
Ensembli | ENSMUST00000002053; ENSMUSP00000002053; ENSMUSG00000001988 ENSMUST00000210748; ENSMUSP00000147412; ENSMUSG00000001988 |
GeneIDi | 18142 |
KEGGi | mmu:18142 |
UCSCi | uc009fhv.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U77967 mRNA Translation: AAB47247.1 |
CCDSi | CCDS20850.1 |
RefSeqi | NP_032744.1, NM_008718.2 XP_017177510.1, XM_017322021.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5SY5 | X-ray | 3.20 | B/D/F | 43-423 | [»] | |
AlphaFoldDBi | P97459 | |||||
SMRi | P97459 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 201818, 1 interactor |
STRINGi | 10090.ENSMUSP00000002053 |
PTM databases
PhosphoSitePlusi | P97459 |
Proteomic databases
PaxDbi | P97459 |
PRIDEi | P97459 |
ProteomicsDBi | 253003 |
Protocols and materials databases
Antibodypediai | 18161, 214 antibodies from 31 providers |
DNASUi | 18142 |
Genome annotation databases
Ensembli | ENSMUST00000002053; ENSMUSP00000002053; ENSMUSG00000001988 ENSMUST00000210748; ENSMUSP00000147412; ENSMUSG00000001988 |
GeneIDi | 18142 |
KEGGi | mmu:18142 |
UCSCi | uc009fhv.2, mouse |
Organism-specific databases
CTDi | 4861 |
MGIi | MGI:109205, Npas1 |
VEuPathDBi | HostDB:ENSMUSG00000001988 |
Phylogenomic databases
eggNOGi | KOG3558, Eukaryota |
GeneTreei | ENSGT00940000161295 |
HOGENOMi | CLU_010044_6_3_1 |
InParanoidi | P97459 |
OMAi | VCQTKNM |
OrthoDBi | 922461at2759 |
PhylomeDBi | P97459 |
TreeFami | TF317772 |
Miscellaneous databases
BioGRID-ORCSi | 18142, 3 hits in 75 CRISPR screens |
PROi | PR:P97459 |
RNActi | P97459, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000001988, Expressed in head and 98 other tissues |
Genevisiblei | P97459, MM |
Family and domain databases
CDDi | cd00130, PAS, 2 hits |
Gene3Di | 4.10.280.10, 1 hit |
InterProi | View protein in InterPro IPR011598, bHLH_dom IPR036638, HLH_DNA-bd_sf IPR000014, PAS IPR035965, PAS-like_dom_sf IPR013767, PAS_fold IPR013655, PAS_fold_3 |
Pfami | View protein in Pfam PF00010, HLH, 1 hit PF00989, PAS, 1 hit PF08447, PAS_3, 1 hit |
SMARTi | View protein in SMART SM00353, HLH, 1 hit SM00091, PAS, 2 hits |
SUPFAMi | SSF47459, SSF47459, 1 hit SSF55785, SSF55785, 2 hits |
TIGRFAMsi | TIGR00229, sensory_box, 1 hit |
PROSITEi | View protein in PROSITE PS50888, BHLH, 1 hit PS50112, PAS, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | NPAS1_MOUSE | |
Accessioni | P97459Primary (citable) accession number: P97459 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 15, 1998 |
Last sequence update: | May 1, 1997 | |
Last modified: | May 25, 2022 | |
This is version 164 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references