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Protein

Mothers against decapentaplegic homolog 5

Gene

Smad5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD5 is a receptor-regulated SMAD (R-SMAD) (By similarity). Required for angiogenesis.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi65ZincBy similarity1
Metal bindingi110ZincBy similarity1
Metal bindingi122ZincBy similarity1
Metal bindingi127ZincBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDevelopmental protein, DNA-binding
Biological processAngiogenesis, Differentiation, Transcription, Transcription regulation
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-201451 Signaling by BMP

Names & Taxonomyi

Protein namesi
Recommended name:
Mothers against decapentaplegic homolog 5
Short name:
MAD homolog 5
Short name:
Mothers against DPP homolog 5
Alternative name(s):
Dwarfin-C
Short name:
Dwf-C
SMAD family member 5
Short name:
SMAD 5
Short name:
Smad5
Short name:
mSmad5
Gene namesi
Name:Smad5
Synonyms:Madh5, Msmad5
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 13

Organism-specific databases

MGIiMGI:1328787 Smad5

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL3883282

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000908662 – 465Mothers against decapentaplegic homolog 5Add BLAST464

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylthreonineBy similarity1
Modified residuei463PhosphoserinePROSITE-ProRule annotationBy similarity1
Modified residuei465PhosphoserinePROSITE-ProRule annotationBy similarity1

Post-translational modificationi

Phosphorylated on serine by BMP (bone morphogenetic proteins) type 1 receptor kinase.
Ubiquitin-mediated proteolysis by SMAD-specific E3 ubiquitin ligase SMURF1.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP97454
PaxDbiP97454
PeptideAtlasiP97454
PRIDEiP97454

PTM databases

iPTMnetiP97454
PhosphoSitePlusiP97454

Expressioni

Gene expression databases

BgeeiENSMUSG00000021540 Expressed in 313 organ(s), highest expression level in ear vesicle
CleanExiMM_SMAD5
GenevisibleiP97454 MM

Interactioni

Subunit structurei

May form trimers with the co-SMAD SMAD4. Interacts with PEBP2-alpha subunit and SMURF1. Interacts with SUV39H1 and SUV39H2. Interacts (via MH2 domain) with LEMD3 (By similarity). Interacts with WWP1. Interacts with TMEM119 (PubMed:21239498). Interacts with ZNF8 (PubMed:12370310). Interacts with RANBP3L (By similarity).By similarity3 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi201278, 13 interactors
IntActiP97454, 2 interactors
MINTiP97454
STRINGi10090.ENSMUSP00000065798

Structurei

Secondary structure

1465
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP97454
SMRiP97454
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini13 – 137MH1PROSITE-ProRule annotationAdd BLAST125
Domaini271 – 465MH2PROSITE-ProRule annotationAdd BLAST195

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi40 – 46Poly-Lys7

Sequence similaritiesi

Belongs to the dwarfin/SMAD family.Curated

Phylogenomic databases

eggNOGiKOG3701 Eukaryota
ENOG410XQKU LUCA
GeneTreeiENSGT00760000119091
HOGENOMiHOG000286018
HOVERGENiHBG053353
InParanoidiP97454
KOiK16790
OMAiDVQPVEY
OrthoDBiEOG091G082C
PhylomeDBiP97454
TreeFamiTF314923

Family and domain databases

Gene3Di2.60.200.10, 1 hit
3.90.520.10, 1 hit
InterProiView protein in InterPro
IPR013790 Dwarfin
IPR003619 MAD_homology1_Dwarfin-type
IPR013019 MAD_homology_MH1
IPR017855 SMAD-like_dom_sf
IPR001132 SMAD_dom_Dwarfin-type
IPR008984 SMAD_FHA_dom_sf
IPR036578 SMAD_MH1_sf
PANTHERiPTHR13703 PTHR13703, 1 hit
PfamiView protein in Pfam
PF03165 MH1, 1 hit
PF03166 MH2, 1 hit
SMARTiView protein in SMART
SM00523 DWA, 1 hit
SM00524 DWB, 1 hit
SUPFAMiSSF49879 SSF49879, 1 hit
SSF56366 SSF56366, 1 hit
PROSITEiView protein in PROSITE
PS51075 MH1, 1 hit
PS51076 MH2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P97454-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MTSMASLFSF TSPAVKRLLG WKQGDEEEKW AEKAVDALVK KLKKKKGAME
60 70 80 90 100
ELEKALSSPG QPSKCVTIPR SLDGRLQVSH RKGLPHVIYC RVWRWPDLQS
110 120 130 140 150
HHELKPLDIC EFPFGSKQKE VCINPYHYKR VESPVLPPVL VPRHNEFNPQ
160 170 180 190 200
HSLLVQFRNL SHNEPHMPQN ATFPDSFHQP NNAPFPLSPN SPYPPSPASS
210 220 230 240 250
TYPNSPASSG PGSPFQLPAD TPPPAYMPPD DQMAPDNSQP MDTSSNMIPQ
260 270 280 290 300
TMPSISSRDV QPVAYEEPKH WCSIVYYELN NRVGEAFHAS STSVLVDGFT
310 320 330 340 350
DPSNNKSRFC LGLLSNVNRN STIENTRRHI GKGVHLYYVG GEVYAECLSD
360 370 380 390 400
SSIFVQSRNC NFHHGFHPTT VCKIPSSCSL KIFNNQEFAQ LLAQSVNHGF
410 420 430 440 450
EAVYELTKMC TIRMSFVKGW GAEYHRQDVT STPCWIEIHL HGPLQWLDKV
460
LTQMGSPLNP ISSVS
Length:465
Mass (Da):52,172
Last modified:May 4, 2001 - v2
Checksum:iD66B638DA76BC20B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti196S → P in AAB39737 (PubMed:9076683).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U58993 mRNA Translation: AAB07871.1
U77638 mRNA Translation: AAB39737.1
AF063006 mRNA Translation: AAC83580.1
AK082997 mRNA Translation: BAC38724.1
BC050001 mRNA Translation: AAH50001.2
CCDSiCCDS26565.1
RefSeqiNP_001157513.1, NM_001164041.1
NP_001157514.1, NM_001164042.1
NP_032567.1, NM_008541.3
XP_006517180.1, XM_006517117.1
XP_006517181.1, XM_006517118.3
UniGeneiMm.272920

Genome annotation databases

EnsembliENSMUST00000069557; ENSMUSP00000065798; ENSMUSG00000021540
ENSMUST00000109874; ENSMUSP00000105500; ENSMUSG00000021540
ENSMUST00000109876; ENSMUSP00000105502; ENSMUSG00000021540
GeneIDi17129
KEGGimmu:17129
UCSCiuc007qsw.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U58993 mRNA Translation: AAB07871.1
U77638 mRNA Translation: AAB39737.1
AF063006 mRNA Translation: AAC83580.1
AK082997 mRNA Translation: BAC38724.1
BC050001 mRNA Translation: AAH50001.2
CCDSiCCDS26565.1
RefSeqiNP_001157513.1, NM_001164041.1
NP_001157514.1, NM_001164042.1
NP_032567.1, NM_008541.3
XP_006517180.1, XM_006517117.1
XP_006517181.1, XM_006517118.3
UniGeneiMm.272920

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5X6GX-ray3.05A/B1-143[»]
5X6HX-ray3.10B1-143[»]
5X6MX-ray3.20A/B/E/F1-143[»]
ProteinModelPortaliP97454
SMRiP97454
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi201278, 13 interactors
IntActiP97454, 2 interactors
MINTiP97454
STRINGi10090.ENSMUSP00000065798

Chemistry databases

ChEMBLiCHEMBL3883282

PTM databases

iPTMnetiP97454
PhosphoSitePlusiP97454

Proteomic databases

MaxQBiP97454
PaxDbiP97454
PeptideAtlasiP97454
PRIDEiP97454

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000069557; ENSMUSP00000065798; ENSMUSG00000021540
ENSMUST00000109874; ENSMUSP00000105500; ENSMUSG00000021540
ENSMUST00000109876; ENSMUSP00000105502; ENSMUSG00000021540
GeneIDi17129
KEGGimmu:17129
UCSCiuc007qsw.2 mouse

Organism-specific databases

CTDi4090
MGIiMGI:1328787 Smad5

Phylogenomic databases

eggNOGiKOG3701 Eukaryota
ENOG410XQKU LUCA
GeneTreeiENSGT00760000119091
HOGENOMiHOG000286018
HOVERGENiHBG053353
InParanoidiP97454
KOiK16790
OMAiDVQPVEY
OrthoDBiEOG091G082C
PhylomeDBiP97454
TreeFamiTF314923

Enzyme and pathway databases

ReactomeiR-MMU-201451 Signaling by BMP

Miscellaneous databases

PROiPR:P97454
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000021540 Expressed in 313 organ(s), highest expression level in ear vesicle
CleanExiMM_SMAD5
GenevisibleiP97454 MM

Family and domain databases

Gene3Di2.60.200.10, 1 hit
3.90.520.10, 1 hit
InterProiView protein in InterPro
IPR013790 Dwarfin
IPR003619 MAD_homology1_Dwarfin-type
IPR013019 MAD_homology_MH1
IPR017855 SMAD-like_dom_sf
IPR001132 SMAD_dom_Dwarfin-type
IPR008984 SMAD_FHA_dom_sf
IPR036578 SMAD_MH1_sf
PANTHERiPTHR13703 PTHR13703, 1 hit
PfamiView protein in Pfam
PF03165 MH1, 1 hit
PF03166 MH2, 1 hit
SMARTiView protein in SMART
SM00523 DWA, 1 hit
SM00524 DWB, 1 hit
SUPFAMiSSF49879 SSF49879, 1 hit
SSF56366 SSF56366, 1 hit
PROSITEiView protein in PROSITE
PS51075 MH1, 1 hit
PS51076 MH2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSMAD5_MOUSE
AccessioniPrimary (citable) accession number: P97454
Secondary accession number(s): P70341, Q810K0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 4, 2001
Last sequence update: May 4, 2001
Last modified: September 12, 2018
This is version 176 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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