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Protein

Four and a half LIM domains protein 1

Gene

Fhl1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

May have an involvement in muscle development or hypertrophy. Isoform 2 binds to RBP-J and plays a negative regulatory role in the RBP-J-mediated transcription in mammalian systems.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri7 – 31C4-typeSequence analysisAdd BLAST25

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDevelopmental protein
Biological processDifferentiation
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Four and a half LIM domains protein 1
Short name:
FHL-1
Alternative name(s):
KyoT
RBP-associated molecule 14-1
Short name:
RAM14-1
Skeletal muscle LIM-protein 1
Short name:
SLIM
Short name:
SLIM-1
Gene namesi
Name:Fhl1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome X

Organism-specific databases

MGIiMGI:1298387 Fhl1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00000757362 – 280Four and a half LIM domains protein 1Add BLAST279

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1
Modified residuei4N6-acetyllysineCombined sources1
Cross-linki86Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Ubl conjugation

Proteomic databases

MaxQBiP97447
PRIDEiP97447

PTM databases

iPTMnetiP97447
PhosphoSitePlusiP97447

Expressioni

Tissue specificityi

Isoform 1 seems to be most abundant in each tissue and isoform 2 much less abundant. Isoform 1 is highly expressed in skeletal muscle and lung, and to a lesser extent in heart, brain and kidney. Isoform 2 was found in brain, lung kidney and genital organs.1 Publication

Gene expression databases

BgeeiENSMUSG00000023092 Expressed in 326 organ(s), highest expression level in muscle of leg
CleanExiMM_FHL1
ExpressionAtlasiP97447 baseline and differential
GenevisibleiP97447 MM

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
RbpjP312664EBI-16082627,EBI-1392666

GO - Molecular functioni

Protein-protein interaction databases

BioGridi199668, 5 interactors
CORUMiP97447
DIPiDIP-42041N
IntActiP97447, 4 interactors
MINTiP97447

Structurei

Secondary structure

1280
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP97447
SMRiP97447
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini40 – 92LIM zinc-binding 1PROSITE-ProRule annotationAdd BLAST53
Domaini101 – 153LIM zinc-binding 2PROSITE-ProRule annotationAdd BLAST53
Domaini162 – 212LIM zinc-binding 3PROSITE-ProRule annotationAdd BLAST51
Domaini221 – 276LIM zinc-binding 4PROSITE-ProRule annotationAdd BLAST56

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri7 – 31C4-typeSequence analysisAdd BLAST25

Keywords - Domaini

LIM domain, Repeat, Zinc-finger

Phylogenomic databases

GeneTreeiENSGT00760000118910
HOGENOMiHOG000231075
HOVERGENiHBG074526
InParanoidiP97447
KOiK14365

Family and domain databases

InterProiView protein in InterPro
IPR001781 Znf_LIM
PfamiView protein in Pfam
PF00412 LIM, 4 hits
SMARTiView protein in SMART
SM00132 LIM, 4 hits
PROSITEiView protein in PROSITE
PS00478 LIM_DOMAIN_1, 4 hits
PS50023 LIM_DOMAIN_2, 4 hits

Sequences (2+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket
Note: Additional isoforms seem to exist.

This entry has 2 described isoforms and 7 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: P97447-1) [UniParc]FASTAAdd to basket
Also known as: KyoT1

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MSEKFDCHYC RDPLQGKKYV QKDGRHCCLK CFDKFCANTC VDCRKPISAD
60 70 80 90 100
AKEVHYKNRY WHDNCFRCAK CLHPLASETF VSKDGKILCN KCATREDSPR
110 120 130 140 150
CKGCFKAIVA GDQNVEYKGT VWHKDCFTCS NCKQVIGTGS FFPKGEDFYC
160 170 180 190 200
VTCHETKFAK HCVKCNKAIT SGGITYQDQP WHAECFVCVT CSKKLAGQRF
210 220 230 240 250
TAVEDQYYCV DCYKNFVAKK CAGCKNPITG FGKGSSVVAY EGQSWHDYCF
260 270 280
HCKKCSVNLA NKRFVFHNEQ VYCPDCAKKL
Length:280
Mass (Da):31,889
Last modified:November 15, 2002 - v3
Checksum:i76C64FB8087BA36D
GO
Isoform 2 (identifier: P97447-2) [UniParc]FASTAAdd to basket
Also known as: KyoT2

The sequence of this isoform differs from the canonical sequence as follows:
     168-194: AITSGGITYQDQPWHAECFVCVTCSKK → GLVKAPVWWPMKDNPGTTTASTAKNAP
     195-280: Missing.

Show »
Length:194
Mass (Da):22,011
Checksum:iDFA2441C2A9673D2
GO

Computationally mapped potential isoform sequencesi

There are 7 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A2AEY2A2AEY2_MOUSE
Four and a half LIM domains 1, isof...
Fhl1 mCG_9696
323Annotation score:
A2AEX6A2AEX6_MOUSE
Four and a half LIM domains protein...
Fhl1
309Annotation score:
A2AEX7A2AEX7_MOUSE
Four and a half LIM domains protein...
Fhl1
210Annotation score:
A2AEX8A2AEX8_MOUSE
Four and a half LIM domains 1, isof...
Fhl1 mCG_9696
296Annotation score:
A2AEY0A2AEY0_MOUSE
Four and a half LIM domains protein...
Fhl1
89Annotation score:
A2AEY1A2AEY1_MOUSE
Four and a half LIM domains protein...
Fhl1
234Annotation score:
A2AEX9A2AEX9_MOUSE
Four and a half LIM domains protein...
Fhl1
72Annotation score:

Sequence cautioni

The sequence AAH29024 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti98S → F in AAC02727 (PubMed:10049693).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_003120168 – 194AITSG…TCSKK → GLVKAPVWWPMKDNPGTTTA STAKNAP in isoform 2. CuratedAdd BLAST27
Alternative sequenceiVSP_003121195 – 280Missing in isoform 2. CuratedAdd BLAST86

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U77039 mRNA Translation: AAC02727.1
U41739 mRNA Translation: AAC02805.1
AF114380 mRNA Translation: AAD53229.1
BC031120 mRNA Translation: AAH31120.1
BC029024 mRNA Translation: AAH29024.1 Different initiation.
CCDSiCCDS30148.1 [P97447-1]
RefSeqiNP_001070830.1, NM_001077362.2
NP_001274729.1, NM_001287800.1 [P97447-1]
NP_034341.2, NM_010211.3 [P97447-1]
XP_006527871.2, XM_006527808.2 [P97447-2]
XP_017173870.1, XM_017318381.1 [P97447-1]
XP_017173871.1, XM_017318382.1 [P97447-2]
UniGeneiMm.3126

Genome annotation databases

EnsembliENSMUST00000023854; ENSMUSP00000023854; ENSMUSG00000023092 [P97447-1]
ENSMUST00000116596; ENSMUSP00000112295; ENSMUSG00000023092 [P97447-1]
GeneIDi14199
KEGGimmu:14199
UCSCiuc009tgm.2 mouse [P97447-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U77039 mRNA Translation: AAC02727.1
U41739 mRNA Translation: AAC02805.1
AF114380 mRNA Translation: AAD53229.1
BC031120 mRNA Translation: AAH31120.1
BC029024 mRNA Translation: AAH29024.1 Different initiation.
CCDSiCCDS30148.1 [P97447-1]
RefSeqiNP_001070830.1, NM_001077362.2
NP_001274729.1, NM_001287800.1 [P97447-1]
NP_034341.2, NM_010211.3 [P97447-1]
XP_006527871.2, XM_006527808.2 [P97447-2]
XP_017173870.1, XM_017318381.1 [P97447-1]
XP_017173871.1, XM_017318382.1 [P97447-2]
UniGeneiMm.3126

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4J2XX-ray2.85B/D168-194[»]
ProteinModelPortaliP97447
SMRiP97447
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi199668, 5 interactors
CORUMiP97447
DIPiDIP-42041N
IntActiP97447, 4 interactors
MINTiP97447

PTM databases

iPTMnetiP97447
PhosphoSitePlusiP97447

Proteomic databases

MaxQBiP97447
PRIDEiP97447

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000023854; ENSMUSP00000023854; ENSMUSG00000023092 [P97447-1]
ENSMUST00000116596; ENSMUSP00000112295; ENSMUSG00000023092 [P97447-1]
GeneIDi14199
KEGGimmu:14199
UCSCiuc009tgm.2 mouse [P97447-1]

Organism-specific databases

CTDi2273
MGIiMGI:1298387 Fhl1

Phylogenomic databases

GeneTreeiENSGT00760000118910
HOGENOMiHOG000231075
HOVERGENiHBG074526
InParanoidiP97447
KOiK14365

Miscellaneous databases

PROiPR:P97447
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000023092 Expressed in 326 organ(s), highest expression level in muscle of leg
CleanExiMM_FHL1
ExpressionAtlasiP97447 baseline and differential
GenevisibleiP97447 MM

Family and domain databases

InterProiView protein in InterPro
IPR001781 Znf_LIM
PfamiView protein in Pfam
PF00412 LIM, 4 hits
SMARTiView protein in SMART
SM00132 LIM, 4 hits
PROSITEiView protein in PROSITE
PS00478 LIM_DOMAIN_1, 4 hits
PS50023 LIM_DOMAIN_2, 4 hits
ProtoNetiSearch...

Entry informationi

Entry nameiFHL1_MOUSE
AccessioniPrimary (citable) accession number: P97447
Secondary accession number(s): O55181, Q8K318
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 15, 2002
Last sequence update: November 15, 2002
Last modified: September 12, 2018
This is version 151 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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Main funding by: National Institutes of Health

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