UniProtKB - P97443 (SMYD1_MOUSE)
Histone-lysine N-methyltransferase Smyd1
Smyd1
Functioni
Methylates histone H3 at 'Lys-4' (H3K4me). Acts as a transcriptional repressor. Essential for cardiomyocyte differentiation and cardiac morphogenesis.
2 PublicationsCatalytic activityi
- L-lysyl4-[histone H3] + 3 S-adenosyl-L-methionine = 3 H+ + N6,N6,N6-trimethyl-L-lysyl4-[histone H3] + 3 S-adenosyl-L-homocysteine1 PublicationEC:2.1.1.3541 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 52 | Zinc 1PROSITE-ProRule annotation | 1 | |
Metal bindingi | 55 | Zinc 1PROSITE-ProRule annotation | 1 | |
Metal bindingi | 65 | Zinc 2PROSITE-ProRule annotation | 1 | |
Metal bindingi | 68 | Zinc 2PROSITE-ProRule annotation | 1 | |
Metal bindingi | 74 | Zinc 1PROSITE-ProRule annotation | 1 | |
Metal bindingi | 78 | Zinc 1PROSITE-ProRule annotation | 1 | |
Metal bindingi | 86 | Zinc 2PROSITE-ProRule annotation | 1 | |
Metal bindingi | 90 | Zinc 2PROSITE-ProRule annotation | 1 | |
Binding sitei | 135 | S-adenosyl-L-methionine | 1 | |
Metal bindingi | 208 | Zinc | 1 | |
Metal bindingi | 274 | Zinc | 1 | |
Metal bindingi | 276 | Zinc | 1 | |
Metal bindingi | 279 | Zinc | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 52 – 90 | MYND-typePROSITE-ProRule annotationAdd BLAST | 39 |
GO - Molecular functioni
- DNA binding Source: UniProtKB-KW
- histone-lysine N-methyltransferase activity Source: GO_Central
- metal ion binding Source: UniProtKB-KW
- transcription corepressor activity Source: MGI
GO - Biological processi
- chromatin remodeling Source: MGI
- heart development Source: MGI
- histone lysine methylation Source: GO_Central
- negative regulation of transcription, DNA-templated Source: MGI
- positive regulation of myoblast differentiation Source: MGI
- positive regulation of myotube differentiation Source: MGI
- skeletal muscle cell differentiation Source: MGI
Keywordsi
Molecular function | DNA-binding, Methyltransferase, Repressor, Transferase |
Biological process | Transcription, Transcription regulation |
Ligand | Metal-binding, S-adenosyl-L-methionine, Zinc |
Names & Taxonomyi
Protein namesi | Recommended name: Histone-lysine N-methyltransferase Smyd1 (EC:2.1.1.3541 Publication)Alternative name(s): CD8b-opposite SET and MYND domain-containing protein 1 Zinc finger protein BOP Short name: m-BOP |
Gene namesi | Name:Smyd1 Synonyms:Bop |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:104790, Smyd1 |
VEuPathDBi | HostDB:ENSMUSG00000055027 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000218308 | 1 – 490 | Histone-lysine N-methyltransferase Smyd1Add BLAST | 490 |
Proteomic databases
MaxQBi | P97443 |
PaxDbi | P97443 |
PRIDEi | P97443 |
ProteomicsDBi | 261586 [P97443-1] 261587 [P97443-2] 261588 [P97443-3] |
PTM databases
iPTMneti | P97443 |
PhosphoSitePlusi | P97443 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSMUSG00000055027, Expressed in skeletal muscle tissue and 222 other tissues |
ExpressionAtlasi | P97443, baseline and differential |
Genevisiblei | P97443, MM |
Interactioni
Subunit structurei
Interacts with HDAC1, HDAC2 and HDAC3.
Interacts (via MYND-type zinc finger) with NACA isoform skNAC.
2 PublicationsProtein-protein interaction databases
BioGRIDi | 198379, 10 interactors |
ELMi | P97443 |
IntActi | P97443, 1 interactor |
STRINGi | 10090.ENSMUSP00000073911 |
Miscellaneous databases
RNActi | P97443, protein |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | P97443 |
SMRi | P97443 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P97443 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 7 – 253 | SETPROSITE-ProRule annotationAdd BLAST | 247 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 17 – 19 | S-adenosyl-L-methionine binding | 3 | |
Regioni | 205 – 206 | S-adenosyl-L-methionine binding | 2 | |
Regioni | 270 – 272 | S-adenosyl-L-methionine bindingBy similarity | 3 |
Domaini
Sequence similaritiesi
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 52 – 90 | MYND-typePROSITE-ProRule annotationAdd BLAST | 39 |
Keywords - Domaini
Zinc-fingerPhylogenomic databases
eggNOGi | KOG2084, Eukaryota |
GeneTreei | ENSGT00940000156114 |
HOGENOMi | CLU_018406_1_1_1 |
InParanoidi | P97443 |
OMAi | YAHGVFP |
OrthoDBi | 981799at2759 |
PhylomeDBi | P97443 |
TreeFami | TF106487 |
Family and domain databases
CDDi | cd10526, SET_SMYD1, 1 hit |
Gene3Di | 1.25.40.10, 1 hit 2.170.270.10, 1 hit |
InterProi | View protein in InterPro IPR001214, SET_dom IPR046341, SET_dom_sf IPR044418, SMYD1_SET IPR011990, TPR-like_helical_dom_sf IPR002893, Znf_MYND |
Pfami | View protein in Pfam PF00856, SET, 1 hit PF01753, zf-MYND, 1 hit |
SMARTi | View protein in SMART SM00317, SET, 1 hit |
SUPFAMi | SSF82199, SSF82199, 1 hit |
PROSITEi | View protein in PROSITE PS50280, SET, 1 hit PS01360, ZF_MYND_1, 1 hit PS50865, ZF_MYND_2, 1 hit |
s (3+)i Sequence
Sequence statusi: Complete.
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 3 described isoforms and 4 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MTIGSMENVE VFTSEGKGRG LKATKEFWAA DVIFAERAYS AVVFDSLINF
60 70 80 90 100
VCHTCFKRQE KLHRCGQCKF AHYCDRTCQK DAWLNHKNEC AAIKKYGKVP
110 120 130 140 150
NENIRLAARI MWRVEREGTG LTEGCLVSVD DLQNHVEHFG EEEQKELRVD
160 170 180 190 200
VDTFLQYWPP QSQQFSMQYI SHIFGVINCN GFTLSDQRGL QAVGVGIFPN
210 220 230 240 250
LGLVNHDCWP NCTVIFNNGN HEAVKSMFHT QMRIELRALG KISEGEELTV
260 270 280 290 300
SYIDFLHLSE ERRRQLKKQY YFDCSCEHCQ KGLKDDLFLA AKEDPKPSQE
310 320 330 340 350
VVKEMIQFSK DTLEKIDKAR SEGLYHEVVK LCRECLEKQE PVFADTNLYV
360 370 380 390 400
LRLLSIASEV LSYLQAYEEA SHYARRMVDG YMKLYHHNNA QLGMAVMRAG
410 420 430 440 450
LTNWHAGHIE VGHGMICKAY AILLVTHGPS HPITKDLEAM RMQTEMELRM
460 470 480 490
FRQNEFMYHK MREAALNNQP MQVMAEPSNE PAPALFHKKQ
Computationally mapped potential isoform sequencesi
There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketG5E8R7 | G5E8R7_MOUSE | Histone-lysine N-methyltransferase ... | Smyd1 | 456 | Annotation score: | ||
Q8BMV4 | Q8BMV4_MOUSE | Histone-lysine N-methyltransferase ... | Smyd1 | 69 | Annotation score: | ||
G3UZ94 | G3UZ94_MOUSE | Histone-lysine N-methyltransferase ... | Smyd1 | 58 | Annotation score: | ||
G3UXZ0 | G3UXZ0_MOUSE | Histone-lysine N-methyltransferase ... | Smyd1 | 21 | Annotation score: |
Sequence cautioni
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural varianti | 95 | K → R in strain: C57BL/6. 1 Publication | 1 | |
Natural varianti | 160 | P → L in strain: C57BL/6. 1 Publication | 1 | |
Natural varianti | 344 | A → T in strain: C57BL/6. 1 Publication | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_050402 | 6 – 45 | MENVE…AVVFD → MKNGEACGGWQ in isoform 3. 1 PublicationAdd BLAST | 40 | |
Alternative sequenceiVSP_050403 | 220 | N → K in isoform 2. 1 Publication | 1 | |
Alternative sequenceiVSP_050404 | 221 – 233 | Missing in isoform 2. 1 PublicationAdd BLAST | 13 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U76371 mRNA Translation: AAC53020.1 U76373 mRNA Translation: AAC53021.2 Different initiation. U76374 mRNA Translation: AAC53022.2 Different initiation. AK142252 mRNA Translation: BAE24995.1 CH466523 Genomic DNA Translation: EDK98934.1 BC076601 mRNA Translation: AAH76601.1 |
CCDSi | CCDS20227.2 [P97443-2] CCDS51805.1 [P97443-1] |
RefSeqi | NP_001153599.1, NM_001160127.1 [P97443-1] NP_033892.2, NM_009762.2 [P97443-2] |
Genome annotation databases
Ensembli | ENSMUST00000074301; ENSMUSP00000073911; ENSMUSG00000055027 [P97443-1] ENSMUST00000114186; ENSMUSP00000109824; ENSMUSG00000055027 [P97443-2] |
GeneIDi | 12180 |
KEGGi | mmu:12180 |
UCSCi | uc009cgj.2, mouse [P97443-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U76371 mRNA Translation: AAC53020.1 U76373 mRNA Translation: AAC53021.2 Different initiation. U76374 mRNA Translation: AAC53022.2 Different initiation. AK142252 mRNA Translation: BAE24995.1 CH466523 Genomic DNA Translation: EDK98934.1 BC076601 mRNA Translation: AAH76601.1 |
CCDSi | CCDS20227.2 [P97443-2] CCDS51805.1 [P97443-1] |
RefSeqi | NP_001153599.1, NM_001160127.1 [P97443-1] NP_033892.2, NM_009762.2 [P97443-2] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3N71 | X-ray | 2.30 | A | 1-490 | [»] | |
AlphaFoldDBi | P97443 | |||||
SMRi | P97443 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 198379, 10 interactors |
ELMi | P97443 |
IntActi | P97443, 1 interactor |
STRINGi | 10090.ENSMUSP00000073911 |
PTM databases
iPTMneti | P97443 |
PhosphoSitePlusi | P97443 |
Proteomic databases
MaxQBi | P97443 |
PaxDbi | P97443 |
PRIDEi | P97443 |
ProteomicsDBi | 261586 [P97443-1] 261587 [P97443-2] 261588 [P97443-3] |
Protocols and materials databases
Antibodypediai | 47498, 232 antibodies from 27 providers |
DNASUi | 12180 |
Genome annotation databases
Ensembli | ENSMUST00000074301; ENSMUSP00000073911; ENSMUSG00000055027 [P97443-1] ENSMUST00000114186; ENSMUSP00000109824; ENSMUSG00000055027 [P97443-2] |
GeneIDi | 12180 |
KEGGi | mmu:12180 |
UCSCi | uc009cgj.2, mouse [P97443-1] |
Organism-specific databases
CTDi | 150572 |
MGIi | MGI:104790, Smyd1 |
VEuPathDBi | HostDB:ENSMUSG00000055027 |
Phylogenomic databases
eggNOGi | KOG2084, Eukaryota |
GeneTreei | ENSGT00940000156114 |
HOGENOMi | CLU_018406_1_1_1 |
InParanoidi | P97443 |
OMAi | YAHGVFP |
OrthoDBi | 981799at2759 |
PhylomeDBi | P97443 |
TreeFami | TF106487 |
Miscellaneous databases
BioGRID-ORCSi | 12180, 0 hits in 76 CRISPR screens |
ChiTaRSi | Smyd1, mouse |
EvolutionaryTracei | P97443 |
PROi | PR:P97443 |
RNActi | P97443, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000055027, Expressed in skeletal muscle tissue and 222 other tissues |
ExpressionAtlasi | P97443, baseline and differential |
Genevisiblei | P97443, MM |
Family and domain databases
CDDi | cd10526, SET_SMYD1, 1 hit |
Gene3Di | 1.25.40.10, 1 hit 2.170.270.10, 1 hit |
InterProi | View protein in InterPro IPR001214, SET_dom IPR046341, SET_dom_sf IPR044418, SMYD1_SET IPR011990, TPR-like_helical_dom_sf IPR002893, Znf_MYND |
Pfami | View protein in Pfam PF00856, SET, 1 hit PF01753, zf-MYND, 1 hit |
SMARTi | View protein in SMART SM00317, SET, 1 hit |
SUPFAMi | SSF82199, SSF82199, 1 hit |
PROSITEi | View protein in PROSITE PS50280, SET, 1 hit PS01360, ZF_MYND_1, 1 hit PS50865, ZF_MYND_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | SMYD1_MOUSE | |
Accessioni | P97443Primary (citable) accession number: P97443 Secondary accession number(s): P97442, P97444, Q6DFW7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 19, 2003 |
Last sequence update: | May 3, 2011 | |
Last modified: | May 25, 2022 | |
This is version 151 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families